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Registro Completo |
Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
Data corrente: |
22/09/2017 |
Data da última atualização: |
10/01/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
LIMA, C. C. V.; AYRES, M. C. C.; PINHEIRO, R. R.; COSTA, J. N.; ANDRIOLI, A.; SOUZA, T. S.; AZEVEDO, D. A. A.; SANTOS, V. W. S.; ARAÚJO, J. F.; SOUSA, A. L. M.; PEIXOTO, R. M.; DAMASCENO, E. M.; COSTA NETO, A. O. |
Afiliação: |
Universidade Federal da Bahia (UFBA) - Salvador, BA, Brasil; UFBA - Salvador, BA, Brasil; RAYMUNDO RIZALDO PINHEIRO, CNPC; Universidade Federal do Recôncavo da Bahia (UFRB) - Cruz das Almas, BA Brasil; ALICE ANDRIOLI, CNPC; UFRB - Cruz das Almas, BA Brasil; Universidade Estadual do Ceará (UFC) - Fortaleza, CE, Brasil; Universidade Federal Rural do Semiárido (UFERSA) - Mossoró, RN, Brasil; Universidade Estadual do Vale do Acaraú (UVA) - Sobral, CE, Brasil; UVA - Sobral, CE, Brasil; UFC - Fortaleza, CE, Brasil; UVA - Sobral, CE, Brasil; Universidade Estadual de Santana (UEFS) - Feira de Santana, BA, Brasil. |
Título: |
Caprine lentivirus in sheep milk and semen. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 69, n. 2, mar./apr. 2017. |
DOI: |
http://dx.doi.org/10.1590/1678-4162-8974 |
Idioma: |
Português |
Notas: |
[ALICE ANDRIOLI PINHEIRO]. |
Conteúdo: |
Abstract: With the objective of detecting the presence of caprine lentivirus (CLV) in ewe milk and in ram semen, ten matrixes and four reproducers experimentally infected with CLV were used. Samples of ewe milk were collected during the four months of lactation, five collections per animal, totaling 50 samples. Regarding the rams, eight semen collections were made per animal, during one year of experimentation, totaling 32 samples. The milk and semen samples were submitted to DNA extraction and the nested polymerase chain reaction test (nPCR) to detect CLV proviral DNA. Eight (16%) of the milk samples were positive in nPCR originating from two ewes. Only one (3.12%) semen sample was positive. The amplification products were sequenced, and were confirmed to be a CLV genomic sequence. Thus, the presence of CLV proviral DNA in sheep milk and semen was demonstrated, confirming the feasibility of infection between species, and alerting to the risk of spreading infections. [Lentivírus caprino em leite e sêmen de ovinos]. Resumo: Com o objetivo de detectar a presença do lentivírus caprino (LVC) no leite de ovelhas e no sêmen de carneiros, utilizaram-se 10 matrizes e quatro reprodutores infectados experimentalmente com o LVC. Foram coletadas amostras de leite das ovelhas durante os quatro meses de lactação, ocorrendo cinco coletas por animal, totalizando 50 amostras. Quanto aos carneiros, realizaram-se oito coletas de sêmen por animal, durante um ano de experimentação, totalizando 32 amostras. As amostras de leite e de sêmen foram submetidas à extração de DNA e à prova de reação em cadeia da polimerase do tipo nested (nPCR) visando à detecção de DNA proviral do LVC. Oito (16%) amostras de leite foram positivas na nPCR oriundas de duas ovelhas. Apenas uma (3,12%) amostra de sêmen apresentou positividade. Produtos da amplificação foram sequenciados, confirmando-se tratar de sequência genômica do LVC. Dessa forma, demonstrou-se a presença do DNA proviral do LVC em leite e sêmen de ovinos, confirmando a viabilidade da infecção entre espécies e, assim, alertando sobre o risco de que a infecção seja disseminada. MenosAbstract: With the objective of detecting the presence of caprine lentivirus (CLV) in ewe milk and in ram semen, ten matrixes and four reproducers experimentally infected with CLV were used. Samples of ewe milk were collected during the four months of lactation, five collections per animal, totaling 50 samples. Regarding the rams, eight semen collections were made per animal, during one year of experimentation, totaling 32 samples. The milk and semen samples were submitted to DNA extraction and the nested polymerase chain reaction test (nPCR) to detect CLV proviral DNA. Eight (16%) of the milk samples were positive in nPCR originating from two ewes. Only one (3.12%) semen sample was positive. The amplification products were sequenced, and were confirmed to be a CLV genomic sequence. Thus, the presence of CLV proviral DNA in sheep milk and semen was demonstrated, confirming the feasibility of infection between species, and alerting to the risk of spreading infections. [Lentivírus caprino em leite e sêmen de ovinos]. Resumo: Com o objetivo de detectar a presença do lentivírus caprino (LVC) no leite de ovelhas e no sêmen de carneiros, utilizaram-se 10 matrizes e quatro reprodutores infectados experimentalmente com o LVC. Foram coletadas amostras de leite das ovelhas durante os quatro meses de lactação, ocorrendo cinco coletas por animal, totalizando 50 amostras. Quanto aos carneiros, realizaram-se oito coletas de sêmen por animal, durante um ano de experimentação, totalizando 3... Mostrar Tudo |
Palavras-Chave: |
Artrite-encefalite Caprina; CAE; Caprine arthritis encephalit virus; Infecção cruzada; Leite de ovelha; Lentivirose; Maedi-Visna; SRLV. |
Thesagro: |
Doença animal; Ovino; Transmissão de doença. |
Thesaurus Nal: |
Animal diseases; Cross infection; Disease transmission; Ewe milk; Lentivirus; Sheep; Visna maedi virus. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/164193/1/CNPC-2017-Caprine.pdf
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Marc: |
LEADER 03603naa a2200505 a 4500 001 2076127 005 2019-01-10 008 2017 bl uuuu u00u1 u #d 024 7 $ahttp://dx.doi.org/10.1590/1678-4162-8974$2DOI 100 1 $aLIMA, C. C. V. 245 $aCaprine lentivirus in sheep milk and semen.$h[electronic resource] 260 $c2017 500 $a[ALICE ANDRIOLI PINHEIRO]. 520 $aAbstract: With the objective of detecting the presence of caprine lentivirus (CLV) in ewe milk and in ram semen, ten matrixes and four reproducers experimentally infected with CLV were used. Samples of ewe milk were collected during the four months of lactation, five collections per animal, totaling 50 samples. Regarding the rams, eight semen collections were made per animal, during one year of experimentation, totaling 32 samples. The milk and semen samples were submitted to DNA extraction and the nested polymerase chain reaction test (nPCR) to detect CLV proviral DNA. Eight (16%) of the milk samples were positive in nPCR originating from two ewes. Only one (3.12%) semen sample was positive. The amplification products were sequenced, and were confirmed to be a CLV genomic sequence. Thus, the presence of CLV proviral DNA in sheep milk and semen was demonstrated, confirming the feasibility of infection between species, and alerting to the risk of spreading infections. [Lentivírus caprino em leite e sêmen de ovinos]. Resumo: Com o objetivo de detectar a presença do lentivírus caprino (LVC) no leite de ovelhas e no sêmen de carneiros, utilizaram-se 10 matrizes e quatro reprodutores infectados experimentalmente com o LVC. Foram coletadas amostras de leite das ovelhas durante os quatro meses de lactação, ocorrendo cinco coletas por animal, totalizando 50 amostras. Quanto aos carneiros, realizaram-se oito coletas de sêmen por animal, durante um ano de experimentação, totalizando 32 amostras. As amostras de leite e de sêmen foram submetidas à extração de DNA e à prova de reação em cadeia da polimerase do tipo nested (nPCR) visando à detecção de DNA proviral do LVC. Oito (16%) amostras de leite foram positivas na nPCR oriundas de duas ovelhas. Apenas uma (3,12%) amostra de sêmen apresentou positividade. Produtos da amplificação foram sequenciados, confirmando-se tratar de sequência genômica do LVC. Dessa forma, demonstrou-se a presença do DNA proviral do LVC em leite e sêmen de ovinos, confirmando a viabilidade da infecção entre espécies e, assim, alertando sobre o risco de que a infecção seja disseminada. 650 $aAnimal diseases 650 $aCross infection 650 $aDisease transmission 650 $aEwe milk 650 $aLentivirus 650 $aSheep 650 $aVisna maedi virus 650 $aDoença animal 650 $aOvino 650 $aTransmissão de doença 653 $aArtrite-encefalite Caprina 653 $aCAE 653 $aCaprine arthritis encephalit virus 653 $aInfecção cruzada 653 $aLeite de ovelha 653 $aLentivirose 653 $aMaedi-Visna 653 $aSRLV 700 1 $aAYRES, M. C. C. 700 1 $aPINHEIRO, R. R. 700 1 $aCOSTA, J. N. 700 1 $aANDRIOLI, A. 700 1 $aSOUZA, T. S. 700 1 $aAZEVEDO, D. A. A. 700 1 $aSANTOS, V. W. S. 700 1 $aARAÚJO, J. F. 700 1 $aSOUSA, A. L. M. 700 1 $aPEIXOTO, R. M. 700 1 $aDAMASCENO, E. M. 700 1 $aCOSTA NETO, A. O. 773 $tArquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte$gv. 69, n. 2, mar./apr. 2017.
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Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
Biblioteca(s): |
Embrapa Instrumentação. |
Data corrente: |
26/09/2023 |
Data da última atualização: |
26/09/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
BIELUCZYK, W.; ASSELTA, F. O.; NAVROSKI, D.; GONTIJO, J. B.; VENTURINI, A. M.; MENDES, L. W.; SIMON, C. P.; CAMARGO, P. B. de; TADINI, A. M.; MARTIN NETO, L.; BENDASSOLLI, J. A.; RODRIGUES, R. R.; van der PUTTEN, H. W.; TSAI, S. M. |
Afiliação: |
Laboratory of Nematology, Wageningen University; Laboratory of Nematology, Wageningen University; University of Sao Paulo, Center for Nuclear Energy in Agriculture, Cell and Molecular Biology Laboratory; University of Sao ˜ Paulo, Center for Nuclear Energy in Agriculture, Cell and Molecular Biology Laboratory; Princeton Institute for International and Regional Studies; University of Sao Paulo, Center for Nuclear Energy in Agriculture, Cell and Molecular Biology Laboratory; University of Sao Paulo, Center for Nuclear Energy in Agriculture, Isotopic Ecology Laboratory; University of Sao Paulo, Center for Nuclear Energy in Agriculture, Isotopic Ecology Laboratory; Brazilian Agricultural Research Corporation, Embrapa Instrumentation; LADISLAU MARTIN NETO, CNPDIA; University of Sao Paulo, Center for Nuclear Energy in Agriculture, Stable Isotope Laboratory; University of Sao Paulo, “Luiz de Queiroz” College of Agriculture, Laboratory of Ecology and Forest Restoration; Laboratory of Nematology, Wageningen University; University of Sao Paulo, Center for Nuclear Energy in Agriculture, Cell and Molecular Biology Laboratory. |
Título: |
Linking above and belowground carbon sequestration, soil organic matter properties, and soil health in Brazilian Atlantic Forest restoration. |
Ano de publicação: |
2023 |
Fonte/Imprenta: |
Journal of Environmental Management, v. 344, 118573, 2023. |
Páginas: |
15 p. |
ISSN: |
0301-4797 |
DOI: |
https://doi.org/10.1016/j.jenvman.2023.118573 |
Idioma: |
Inglês |
Conteúdo: |
Forest restoration mitigates climate change by removing CO2 and storing C in terrestrial ecosystems. However, incomplete information on C storage in restored tropical forests often fails to capture the ecosystem?s holistic C dynamics. This study provides an integrated assessment of C storage in above to belowground subsystems, its consequences for greenhouse gas (GHG) fluxes, and the quantity, quality, and origin of soil organic matter (SOM) in restored Atlantic forests in Brazil. Relations between SOM properties and soil health indicators were also explored. We examined two restorations using tree planting (?active restoration?): an 8-year-old forest with green manure and native trees planted in two rounds, and a 15-year-old forest with native-planted trees in one round without green manure. Restorations were compared to reformed pasture and primary forest sites. We measured C storage in soil layers (0?10, 10?20, and 20?30 cm), litter, and plants. GHG emissions were assessed using CH4 and CO2 fluxes. SOM quantity was evaluated using C and N, quality using humification index (HLIFS), and origin using δ13C and δ15N. Nine soil health indicators were interrelated with SOM attributes. The primary forest presented the highest C stocks (107.7 Mg C ha− 1 ), followed by 15- and 8-year-old restorations and pasture with 69.8, 55.5, and 41.8 Mg C ha− 1 , respectively. Soil C stocks from restorations and pasture were 20% lower than primary forest. However, 8- and 15-year-old restorations stored 12.3 and 28.3 Mg ha− 1 more aboveground C than pasture. The younger forest had δ13C and δ15N values of 2.1 and 1.7?, respectively, lower than the 15-year-old forest, indicating more C derived from C3 plants and biological N fixation. Both restorations and pasture had at least 34% higher HLIFS in deeper soil layers (10?30 cm) than primary forest, indicating a lack of labile SOM. Native and 15-year-old forests exhibited higher soil methane influx (141.1 and 61.9 μg m− 2 h− 1 ). Forests outperformed pasture in most soil health indicators, with 69% of their variance explained by SOM properties. However, SOM quantity and quality regeneration in both restorations approached the pristine forest state only in the top 10 cm layer, while deeper soil retained agricultural degradation legacies. In conclusion, active restoration of the Atlantic Forest is a superior approach compared to pasture reform for GHG mitigation. Nonetheless, the development of restoration techniques to facilitate labile C input into deeper soil layers (>10 cm) is needed to further improve soil multifunctionality and long-term C storage. MenosForest restoration mitigates climate change by removing CO2 and storing C in terrestrial ecosystems. However, incomplete information on C storage in restored tropical forests often fails to capture the ecosystem?s holistic C dynamics. This study provides an integrated assessment of C storage in above to belowground subsystems, its consequences for greenhouse gas (GHG) fluxes, and the quantity, quality, and origin of soil organic matter (SOM) in restored Atlantic forests in Brazil. Relations between SOM properties and soil health indicators were also explored. We examined two restorations using tree planting (?active restoration?): an 8-year-old forest with green manure and native trees planted in two rounds, and a 15-year-old forest with native-planted trees in one round without green manure. Restorations were compared to reformed pasture and primary forest sites. We measured C storage in soil layers (0?10, 10?20, and 20?30 cm), litter, and plants. GHG emissions were assessed using CH4 and CO2 fluxes. SOM quantity was evaluated using C and N, quality using humification index (HLIFS), and origin using δ13C and δ15N. Nine soil health indicators were interrelated with SOM attributes. The primary forest presented the highest C stocks (107.7 Mg C ha− 1 ), followed by 15- and 8-year-old restorations and pasture with 69.8, 55.5, and 41.8 Mg C ha− 1 , respectively. Soil C stocks from restorations and pasture were 20% lower than primary forest. However, 8- and 1... Mostrar Tudo |
Palavras-Chave: |
Ecosystem restoration; Greenhouse gas; Soil health; SOM humification index. |
Categoria do assunto: |
-- |
Marc: |
LEADER 03790naa a2200361 a 4500 001 2156894 005 2023-09-26 008 2023 bl uuuu u00u1 u #d 022 $a0301-4797 024 7 $ahttps://doi.org/10.1016/j.jenvman.2023.118573$2DOI 100 1 $aBIELUCZYK, W. 245 $aLinking above and belowground carbon sequestration, soil organic matter properties, and soil health in Brazilian Atlantic Forest restoration.$h[electronic resource] 260 $c2023 300 $a15 p. 520 $aForest restoration mitigates climate change by removing CO2 and storing C in terrestrial ecosystems. However, incomplete information on C storage in restored tropical forests often fails to capture the ecosystem?s holistic C dynamics. This study provides an integrated assessment of C storage in above to belowground subsystems, its consequences for greenhouse gas (GHG) fluxes, and the quantity, quality, and origin of soil organic matter (SOM) in restored Atlantic forests in Brazil. Relations between SOM properties and soil health indicators were also explored. We examined two restorations using tree planting (?active restoration?): an 8-year-old forest with green manure and native trees planted in two rounds, and a 15-year-old forest with native-planted trees in one round without green manure. Restorations were compared to reformed pasture and primary forest sites. We measured C storage in soil layers (0?10, 10?20, and 20?30 cm), litter, and plants. GHG emissions were assessed using CH4 and CO2 fluxes. SOM quantity was evaluated using C and N, quality using humification index (HLIFS), and origin using δ13C and δ15N. Nine soil health indicators were interrelated with SOM attributes. The primary forest presented the highest C stocks (107.7 Mg C ha− 1 ), followed by 15- and 8-year-old restorations and pasture with 69.8, 55.5, and 41.8 Mg C ha− 1 , respectively. Soil C stocks from restorations and pasture were 20% lower than primary forest. However, 8- and 15-year-old restorations stored 12.3 and 28.3 Mg ha− 1 more aboveground C than pasture. The younger forest had δ13C and δ15N values of 2.1 and 1.7?, respectively, lower than the 15-year-old forest, indicating more C derived from C3 plants and biological N fixation. Both restorations and pasture had at least 34% higher HLIFS in deeper soil layers (10?30 cm) than primary forest, indicating a lack of labile SOM. Native and 15-year-old forests exhibited higher soil methane influx (141.1 and 61.9 μg m− 2 h− 1 ). Forests outperformed pasture in most soil health indicators, with 69% of their variance explained by SOM properties. However, SOM quantity and quality regeneration in both restorations approached the pristine forest state only in the top 10 cm layer, while deeper soil retained agricultural degradation legacies. In conclusion, active restoration of the Atlantic Forest is a superior approach compared to pasture reform for GHG mitigation. Nonetheless, the development of restoration techniques to facilitate labile C input into deeper soil layers (>10 cm) is needed to further improve soil multifunctionality and long-term C storage. 653 $aEcosystem restoration 653 $aGreenhouse gas 653 $aSoil health 653 $aSOM humification index 700 1 $aASSELTA, F. O. 700 1 $aNAVROSKI, D. 700 1 $aGONTIJO, J. B. 700 1 $aVENTURINI, A. M. 700 1 $aMENDES, L. W. 700 1 $aSIMON, C. P. 700 1 $aCAMARGO, P. B. de 700 1 $aTADINI, A. M. 700 1 $aMARTIN NETO, L. 700 1 $aBENDASSOLLI, J. A. 700 1 $aRODRIGUES, R. R. 700 1 $avan der PUTTEN, H. W. 700 1 $aTSAI, S. M. 773 $tJournal of Environmental Management$gv. 344, 118573, 2023.
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