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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
15/04/2004 |
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Data da última atualização: |
15/04/2004 |
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Autoria: |
LEGRIS, G.; POTRYKUS, I.; PUONTI-KAERLAS, J. |
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Título: |
Optimising conditions for biolistic transformation of cassava. |
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Ano de publicação: |
1998 |
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Fonte/Imprenta: |
Revista Brasileira de Mandioca, Cruz das Almas, v. 17, p. 31, nov., 1998. Suplemento. |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: Particle bombardment offers a tissue- and genotype-independente transformation technique that can be used to circumvent host strain restrictions sometimes encountered when using Agrobacterium-based transformation methods. We have previously developed an Agrobacterium-based transformation system for cassava. Now we present a novel protocol for Particle Inflow Gun (PIG) - mediated transformation, developed for use in combination with the organogenesis-based shoot regeneration system from somatic cotyledons. The parameters for transformation conditions have been partially optimised using transient GUS assays. The in vitro performance of different cassava cultivars after particle bombardment has been evaluated, and the frequencies of plant regeneration after bombardment and selection in cassava have been significantly improved by optimising the protocols further. The parameters partially optimised so far include the amount of DNA used, the flying distance of the microprojectiles, the duration of pre-and postplasmolysis treatments and the composition of the regeneration medium used after bombardment. |
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Categoria do assunto: |
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Marc: |
LEADER 01554naa a2200145 a 4500
001 1651901
005 2004-04-15
008 1998 bl uuuu u00u1 u #d
100 1 $aLEGRIS, G.
245 $aOptimising conditions for biolistic transformation of cassava.
260 $c1998
520 $aAbstract: Particle bombardment offers a tissue- and genotype-independente transformation technique that can be used to circumvent host strain restrictions sometimes encountered when using Agrobacterium-based transformation methods. We have previously developed an Agrobacterium-based transformation system for cassava. Now we present a novel protocol for Particle Inflow Gun (PIG) - mediated transformation, developed for use in combination with the organogenesis-based shoot regeneration system from somatic cotyledons. The parameters for transformation conditions have been partially optimised using transient GUS assays. The in vitro performance of different cassava cultivars after particle bombardment has been evaluated, and the frequencies of plant regeneration after bombardment and selection in cassava have been significantly improved by optimising the protocols further. The parameters partially optimised so far include the amount of DNA used, the flying distance of the microprojectiles, the duration of pre-and postplasmolysis treatments and the composition of the regeneration medium used after bombardment.
700 1 $aPOTRYKUS, I.
700 1 $aPUONTI-KAERLAS, J.
773 $tRevista Brasileira de Mandioca, Cruz das Almas$gv. 17, p. 31, nov., 1998. Suplemento.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
14/04/2008 |
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Data da última atualização: |
14/04/2008 |
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Autoria: |
MARTIN-NETO, L.; BAYER, C. |
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Título: |
Sequestro de carbono pelo solo e o plantio direto. |
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Ano de publicação: |
2000 |
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Fonte/Imprenta: |
Boletim Informativo da Sociedade Brasileira de Ciência do Solo, Viçosa- MG, v. 25, n. 2, p. 11-14 , 2000. |
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Idioma: |
Português |
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Categoria do assunto: |
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Marc: |
LEADER 00392naa a2200121 a 4500
001 1030903
005 2008-04-14
008 2000 bl --- 0-- u #d
100 1 $aMARTIN-NETO, L.
245 $aSequestro de carbono pelo solo e o plantio direto.
260 $c2000
700 1 $aBAYER, C.
773 $tBoletim Informativo da Sociedade Brasileira de Ciência do Solo, Viçosa- MG$gv. 25, n. 2, p. 11-14 , 2000.
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Embrapa Instrumentação (CNPDIA) |
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