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 | Acesso ao texto completo restrito à biblioteca da Embrapa Agrobiologia. Para informações adicionais entre em contato com cnpab.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Agrobiologia. |
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Data corrente: |
22/04/2008 |
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Data da última atualização: |
16/03/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ROUWS, L. F. M.; SIMÕES ARAÚJO, J. L.; HEMERLY, A. S.; BALDANI, J. I. |
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Afiliação: |
Luc F. M. Rouws, UFRRJ; Jean Luis Simões Araújo, Embrapa Agrobiologia; Adriana S. Hemerly, Instituto de Pesquisas do Jardim Botânico do RJ; José Ivo Baladani, Embrapa Agrobiologia. |
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Título: |
Validation of a Tn5 transposon mutagenesis system for Gluconacetobacter diazotrophicus through characterization of a flagellar mutant. |
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Ano de publicação: |
2008 |
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Fonte/Imprenta: |
Archives of Microbiology, New York, v. 189, n. 4, p. 397-405, apr. 2008. |
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Idioma: |
Inglês |
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Notas: |
Parceria: UFRRJ; Instituto de Pesquisas do Jardim Botânico do Rio de Janeiro. |
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Conteúdo: |
Gluconacetobacter diazotrophicus is a nitrogen-fixing bacterium, which was originally isolated from the interior of sugarcane plants. The genome of strain PAL5 of G. diazotrophicus has been completely sequenced and a next step is the functional characterization of its genes. The aim of this study was to establish an efficient mutagenesis method, using the commercial Tn5 transposon EZ::Tn5?Tnp Transposome? (Epicentre). Up to 1 × 106 mutants per microgram of transposome were generated in a single electroporation experiment. Insertion-site flanking sequences were amplified by inverse PCR and sequenced for 31 mutants. For ten of these mutants, both insertion flanks could be identified, confirming the 9 bp duplication that is typical for Tn5 transposition. Insertions occurred in a random fashion and were genetically stable for at least 50 generations. One mutant had an insertion in a homolog of the flagellar gene flgA, and was therefore predicted to be affected in flagella-dependent traits and used to validate the applied mutagenesis methodology. This mutant lacked flagella and was non-motile on soft agar. Interestingly, it was also strongly affected in the ability to form biofilm on glass wool. |
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Palavras-Chave: |
Bactéria endofítica. |
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Thesaurus Nal: |
Gluconacetobacter diazotrophicus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01936naa a2200193 a 4500
001 1626792
005 2015-03-16
008 2008 bl uuuu u00u1 u #d
100 1 $aROUWS, L. F. M.
245 $aValidation of a Tn5 transposon mutagenesis system for Gluconacetobacter diazotrophicus through characterization of a flagellar mutant.
260 $c2008
500 $aParceria: UFRRJ; Instituto de Pesquisas do Jardim Botânico do Rio de Janeiro.
520 $aGluconacetobacter diazotrophicus is a nitrogen-fixing bacterium, which was originally isolated from the interior of sugarcane plants. The genome of strain PAL5 of G. diazotrophicus has been completely sequenced and a next step is the functional characterization of its genes. The aim of this study was to establish an efficient mutagenesis method, using the commercial Tn5 transposon EZ::Tn5?<KAN-2>Tnp Transposome? (Epicentre). Up to 1 × 106 mutants per microgram of transposome were generated in a single electroporation experiment. Insertion-site flanking sequences were amplified by inverse PCR and sequenced for 31 mutants. For ten of these mutants, both insertion flanks could be identified, confirming the 9 bp duplication that is typical for Tn5 transposition. Insertions occurred in a random fashion and were genetically stable for at least 50 generations. One mutant had an insertion in a homolog of the flagellar gene flgA, and was therefore predicted to be affected in flagella-dependent traits and used to validate the applied mutagenesis methodology. This mutant lacked flagella and was non-motile on soft agar. Interestingly, it was also strongly affected in the ability to form biofilm on glass wool.
650 $aGluconacetobacter diazotrophicus
653 $aBactéria endofítica
700 1 $aSIMÕES ARAÚJO, J. L.
700 1 $aHEMERLY, A. S.
700 1 $aBALDANI, J. I.
773 $tArchives of Microbiology, New York$gv. 189, n. 4, p. 397-405, apr. 2008.
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Registro original: |
Embrapa Agrobiologia (CNPAB) |
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Registro Completo
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
13/09/2005 |
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Data da última atualização: |
13/09/2005 |
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Autoria: |
MARTIN, A. R.; TORIZ, G. G.; MANOLACHE, S.; DENES, F.; MATTOSO, L. H. C. |
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Título: |
AFM characterization of sisal fibers modified by cold plasma chemistry. |
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Ano de publicação: |
2001 |
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Fonte/Imprenta: |
Acta Microscopica, Maracaibo, v. 10, p. 68-73. abr 2001. Supplement 1. Proceedings of The First Latin American Symposium on Scanning Microscopy, São Pedro, SP, april, 2001. |
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Idioma: |
Inglês |
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Conteúdo: |
Atomic Force Microscopy (AFM) can be successfully used to quantify the surface roughness of sisal fibers treated under dichlorosilane (DS) cold plasma conditions. |
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Palavras-Chave: |
Fibras de Sisal; Microscopia de força atômica; Plasma frio. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00856naa a2200205 a 4500
001 1029076
005 2005-09-13
008 2001 bl --- 0-- u #d
100 1 $aMARTIN, A. R.
245 $aAFM characterization of sisal fibers modified by cold plasma chemistry.
260 $c2001
520 $aAtomic Force Microscopy (AFM) can be successfully used to quantify the surface roughness of sisal fibers treated under dichlorosilane (DS) cold plasma conditions.
653 $aFibras de Sisal
653 $aMicroscopia de força atômica
653 $aPlasma frio
700 1 $aTORIZ, G. G.
700 1 $aMANOLACHE, S.
700 1 $aDENES, F.
700 1 $aMATTOSO, L. H. C.
773 $tActa Microscopica, Maracaibo$gv. 10, p. 68-73. abr 2001. Supplement 1. Proceedings of The First Latin American Symposium on Scanning Microscopy, São Pedro, SP, april, 2001.
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Embrapa Instrumentação (CNPDIA) |
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