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| 2. |  | SILVA-NETA, L. C.; PINHO, E. V.; VEIGA, A. D.; PINHO, R. G.; GUIMARÃES, R. M.; CAIXETA, F.; SANTOS, H. O.; MARQUES, T. L. Expression of genes related to tolerance to low temperature for maize seed germination. Genetics and Molecular Research, v. 14, n, 1, p. 2674-2690, 2015.| Biblioteca(s): Embrapa Cerrados. |
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| Registros recuperados : 2 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Cerrados. Para informações adicionais entre em contato com cpac.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Cerrados. |
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Data corrente: |
11/02/2016 |
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Data da última atualização: |
12/02/2016 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
SILVA-NETA, L. C.; PINHO, E. V.; VEIGA, A. D.; PINHO, R. G.; GUIMARÃES, R. M.; CAIXETA, F.; SANTOS, H. O.; MARQUES, T. L. |
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Afiliação: |
ADRIANO DELLY VEIGA, CPAC. |
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Título: |
Expression of genes related to tolerance to low temperature for maize seed germination. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Genetics and Molecular Research, v. 14, n, 1, p. 2674-2690, 2015. |
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DOI: |
http://dx.doi.org/10.4238/2015.March.30.28 |
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Idioma: |
Português |
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Conteúdo: |
ABSTRACT. The aim of this study was to characterize maize lines tolerant to cold temperatures during the germination process. Seeds from lines with different levels of tolerance to low temperatures were used; 3 lines were classified as tolerant and 3 as susceptible to low germination temperatures. A field was set up to multiply seeds from selected lines. After the seeds were harvested and classified, we conducted physiological tests and analyzed fatty acid content of palmitic, stearic, oleic, linoleic, linolenic, and eicosenoic acids. In proteomic analysis, the expression of heat-resistant proteins, including catalase, peroxidase, esterase, superoxide dismutase, and α-amylase, were evaluated. Transcript analysis was used to measure the expression of the genes AOX1, AOX2, ZmMPK-17, and ZmAN-13. The material showing the highest susceptibility to low germination temperatures contained high saturated fatty acid content. Expression of α-amylase in seeds soaked for 72 h at a temperature of 10°C was lower than expression of α-amylase when soaked at 25°C for the same amount of time. We observed variation in the expression of heat-resistant proteins |
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Palavras-Chave: |
Ácidos graxos; Estresse abiótico. |
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Thesagro: |
Germinação; Semente; Zea Mays. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01973naa a2200277 a 4500
001 2036485
005 2016-02-12
008 2015 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.4238/2015.March.30.28$2DOI
100 1 $aSILVA-NETA, L. C.
245 $aExpression of genes related to tolerance to low temperature for maize seed germination.
260 $c2015
520 $aABSTRACT. The aim of this study was to characterize maize lines tolerant to cold temperatures during the germination process. Seeds from lines with different levels of tolerance to low temperatures were used; 3 lines were classified as tolerant and 3 as susceptible to low germination temperatures. A field was set up to multiply seeds from selected lines. After the seeds were harvested and classified, we conducted physiological tests and analyzed fatty acid content of palmitic, stearic, oleic, linoleic, linolenic, and eicosenoic acids. In proteomic analysis, the expression of heat-resistant proteins, including catalase, peroxidase, esterase, superoxide dismutase, and α-amylase, were evaluated. Transcript analysis was used to measure the expression of the genes AOX1, AOX2, ZmMPK-17, and ZmAN-13. The material showing the highest susceptibility to low germination temperatures contained high saturated fatty acid content. Expression of α-amylase in seeds soaked for 72 h at a temperature of 10°C was lower than expression of α-amylase when soaked at 25°C for the same amount of time. We observed variation in the expression of heat-resistant proteins
650 $aGerminação
650 $aSemente
650 $aZea Mays
653 $aÁcidos graxos
653 $aEstresse abiótico
700 1 $aPINHO, E. V.
700 1 $aVEIGA, A. D.
700 1 $aPINHO, R. G.
700 1 $aGUIMARÃES, R. M.
700 1 $aCAIXETA, F.
700 1 $aSANTOS, H. O.
700 1 $aMARQUES, T. L.
773 $tGenetics and Molecular Research$gv. 14, n, 1, p. 2674-2690, 2015.
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Embrapa Cerrados (CPAC) |
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