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Registros recuperados : 225 | |
41. | | MOLINARI, M. D. C.; BARBOSA, D. A.; MARIN, S. R. R.; ANDREATTA, E. C.; FUGANTI-PAGLIARINI, R.; MERTZ-HENNING, L. M.; HARMON, F.; NEPOMUCENO, A. L. Análise in sílico de splicing alternativo em Arabidopsis thaliana DBR1 mutante. In: CONGRESO DE LA SOJA DEL MERCOSUR, 7.; A TODA SOJA, 2019, Rosario. Reformular la Soja para Impulsar una Cadena de Conocimiento: [anais]. Rosario: ACSOJA, 2019. MERCOSOJA, 2019. Biblioteca(s): Embrapa Soja. |
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42. | | MARIN, D. R.; SOUZA, E. M. G.; MOLINARI, M. D. C.; MARIN, S. R. R.; JOAQUIM, P. I. L.; NEPOMUCENO, A. L.; MERTZ-HENNING, L. M. Análise in silico de transportadores de aminoácidos diferencialmente expressos em sementes de cultivares de soja com variação na coloração do tegumento. In: JORNADA ACADÊMICA DA EMBRAPA SOJA, 15., 2020, Londrina. Resumos expandidos... Londrina: Embrapa Soja, 2020. 244 p. (Embrapa Soja. Documentos, 429). p. 119-126 (Embrapa Soja. Documentos, 429). Artigo de acesso aberto. Biblioteca(s): Embrapa Soja. |
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43. | | MARTINS, P. K.; JORDÃO, B. Q.; BRETON, M. C.; PEDROSO, J. C.; MARIN, S. R. R.; BINNECK, E.; OYA, T.; NEPOMUCENO, A. L. Avaliação dos efeitos do déficit hídrico na expressão gênica em raízes de soja [Glycine max (l.) Merrill] In: CONGRESSO NACIONAL DE GENÉTICA, 48., 2002, Águas de Lindóia. A genética na inclusão social: resumos. Águas de Lindóia: SBG, 2002. 1 CD-ROM. Resumo Área MG pdf.091. Biblioteca(s): Embrapa Soja. |
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44. | | ZIOBER, I. L.; PAIÃO, F. G.; MARIN, S. R. R.; MARCHI, D. F.; BINNECK, E.; NEPOMUCENO, A. L.; COUTINHO, L. L.; SHIMOKOMAKI, M. Molecular cloning of ?RYR hotspot region 1 from broiler chicken. Brazilian Archives of Biology and Technology, Curitiba, v. 52, Special number, p. 225-231, nov. 2009. Biblioteca(s): Embrapa Soja. |
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45. | | BROGIN, R. L.; ARIAS, C. A. A.; VELLO, N. A.; TOLEDO, J. F. F. de; PÍPOLO, A. E.; CATELLI, L. L.; MARIN, S. R. R. Molecular mapping of a gene conferring resistance to soybean rust. In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. p. 318. (Embrapa Soja. Documentos, 228). Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. Biblioteca(s): Embrapa Soja. |
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46. | | ROLLA, A. A. P.; BENEVENTI, M. A.; FUGANTI, R.; MARIN, S. R. R.; FARIAS, J. R. B.; ABDELNOOR, R. V.; NEPOMUCENO, A. L.; MARCELINO, F. C. Method validation for quantification of transgene copy number in soybean genome using qPCR relative quantification by 2 -^^ Ct or 2 ^ Ct formule. In: WORLD SOYBEAN RESEARCH CONFERENCE, 8., 2009, Beijing. Developing a global soy blueprint for a safe secure and sustainable supply: abstracts. Beijing: Chinese Academy of Agricultural Sciences: Institute of Crop Science, 2009. p. 233, ref. P581. WSRC 2009. Editado por Lijuan Qiu, Rongxia Guan, Jian Jin, Qijan Song, Shuntang Guo, Wenbin Li, Yuanchao Wang, Tianfu Han, Xiaobing Liu, Deyue Yu, Lianzhou Jiang, Deliang Peng. Biblioteca(s): Embrapa Soja. |
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47. | | MARINHO, J. P.; COUTINHO, I. D.; LAMEIRO, R. F.; MARIN, S. R. R.; COLNAGO, L. A.; NAKASHIMA, K.; YAMAGUCH-SHINOZAKI, K.; NEPOMUCENO, A. L.; MERTZ-HENNING, L. M. Metabolic alterations in conventional and genetically modified soybean plants with GmDREB2A;2 FL and GmDREB2A;2 CA transcription factors during water deficit. Plant Physiology and Biochemistry v. 140, 2019. 122-135 Biblioteca(s): Embrapa Instrumentação; Embrapa Soja. |
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48. | | FUGANTI, R.; BENEVENTI, M. A.; SILVA, J. F. V.; ARIAS, C. A. A.; MARIN, S. R. R.; BINNECK, E.; NEPOMUCENO, A. L. Microsatellite insertion in a heat-shock protein-promoter region (Gmhsp 17.6-L) in soybean plants resistant and susceptible to Meloidogyne javanica. In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. p. 321. (Embrapa Soja. Documentos, 228). Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. Biblioteca(s): Embrapa Soja. |
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49. | | BENEVENTI, M. A.; FUGANTI, R.; SILVA, J. F. V.; ARIAS, C. A. A.; MARIN, S. R. R.; BINNECK, E.; LIMA, C. G.; NEPOMUCENO, A. L. Microsatellite molecular markers associated with selection process of soybean resistant genotypes to Meloidogyne javanica and Heterodera glycines. In: WORLD SOYBEAN RESEARCH CONFERENCE, 7.; INTERNATIONAL SOYBEAN PROCESSING AND UTILIZATION CONFERENCE, 4.; CONGRESSO BRASILEIRO DE SOJA, 3., 2004, Foz do Iguassu. Abstracts of contributed papers and posters. Londrina: Embrapa Soybean, 2004. p. 321-322. (Embrapa Soja. Documentos, 228). Editado por Flávio Moscardi, Clara Beatriz Hoffmann-Campo, Odilon Ferreira Saraiva, Paulo Roberto Galerani, Francisco Carlos Krzyzanowski, Mercedes Concordia Carrão-Panizzi. Biblioteca(s): Embrapa Soja. |
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50. | | ALMEIDA, A. M. R.; BINNECK, E.; PIUGA, F. F.; MARIN, S. R. R.; VALLE, P. R. Z. R. do; SILVEIRA, C. A. Characterization of powdery mildews strains from soybean, bean, sunflower, and weeds in Brazil using rDNA-ITS sequences. Tropical Plant Pathology, Brasília, DF, v. 33, n. 1, p. 20-26. jan./fev. 2008. Biblioteca(s): Embrapa Soja. |
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51. | | ARIAS, C. A. A.; BONATO, A. L. V.; MARIN, S. R. R.; MOREIRA, M. A.; ALMEIDA, L. A. de; CATTELI, L. L. Caracterização do germoplasma ativo de soja com marcadores moleculares tipo AFLP e Microssatélites (04.2000.322-03). In: HOFFMANN-CAMPO, C. B.; SARAIVA, O. F. (Org.). Resultados de pesquisa da Embrapa Soja - 2002: ecofisiologia e biologia molecular. Londrina: Embrapa Soja, 2003. p. 27-31. (Embrapa Soja. Documentos, 217). Biblioteca(s): Embrapa Soja. |
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52. | | ARIAS, C. A. A.; BONATO, A. L. V.; MARIN, S. R. R.; MOREIRA, M. A.; ALMEIDA, L. A. de; CATTELI, L. L. Caracterização do germoplasma ativo de soja com marcadores moleculares tipo AFLP e microssatélites. In: HOFFMANN-CAMPO, C. B.; SARAIVA, O. F. (Org.). Resultados de pesquisa da Embrapa Soja - 2001: ecofisiologia e biologia molecular. Londrina: Embrapa Soja, 2002. p. 36-37. (Embrapa Soja. Documentos, 198). Biblioteca(s): Embrapa Soja. |
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53. | | BARBOSA, D. de A.; MOLINARI, M. D. C.; FUGANTI-PAGLIARINI, R.; KAFER, J. M.; MARIN, D. C.; MARIN, S. R. R.; MERTZ-HENNING, L. M.; NEPOMUCENO, A. L. Caracterização in silico de genes drip em Glycine max e Glycine soja. In: CONGRESSO BRASILEIRO DE SOJA, 9., 2022, Foz do iguaçu, PR. Desafios para a produtividade sustentável no Mercosul: resumos. Brasília, DF: Embrapa, 2022. Regina Maria Villas Bôas de Campos Leite, Adeney de Freitas Bueno, editores técnicos. resumo 144. p. 164. Biblioteca(s): Embrapa Soja. |
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54. | | CARANHATO, A. L. H.; BARBOSA, D. de A.; FUHRMANN, M. B.; MARINHO, J. P.; MARIN, S. R. R.; MELO, C. L. P. de; NEPOMUCENO, A. L. Avaliação da segregação de híbridos de soja com uma linhagem geneticamente modificada tolerante à seca. In: CONGRESSO BRASILEIRO DE MELHORAMENTO DE PLANTAS, 9., 2017, Foz do Iguaçu. Melhoramento de plantas: projetando o futuro: anais. Maringá: SBMP, 2017. v. 1. E-book. p. 160. Biblioteca(s): Embrapa Soja. |
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55. | | LEITE, J. P.; BARBOSA, E. G. G; MARIN, S. R. R.; MARINHO, J. P.; FUGANTI-PAGLIARINI, R.; YAMAGUCHI-SHINOZAKI, K.; NEPOMUCENO, A. L.; DESIDÉRIO, J. A. Caracterização de plantas transgênicas de soja obtidas com a forma constitutiva do fator de transcrição AREB1. In: CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá. Soja: integração nacional e desenvolvimento sustentável: resumos. Brasília, DF: Embrapa, 2012. p. 14, res. 6. Biblioteca(s): Embrapa Soja. |
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56. | | LEITE, J. P.; BARBOSA, E. G. G; MARIN, S. R. R.; MARINHO, J. P.; FUGANTI-PAGLIARINI, R.; YAMAGUCHI-SHINOZAKI, K.; NEPOMUCENO, A. L.; DESIDÉRIO, J. A. Caracterização de plantas transgênicas de soja obtidas com a forma constitutiva do fator de transcrição AREB1. In: CONGRESSO BRASILEIRO DE SOJA, 6., 2012, Cuiabá. Soja: integração nacional e desenvolvimento sustentável: anais. Brasília, DF: Embrapa, 2012. 5 p. 1 CD-ROM. Biblioteca(s): Embrapa Soja. |
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57. | | ALMEIDA, A. M. R.; VLIET, H. van der; KITAJIMA, E. W.; PIUGA, F. F.; MARIN, S. R. R.; BINNECK, E.; NEPOMUCENO, A. L. Necrose da haste: nova virose da soja no Brasil. In: CONGRESSO BRASILEIRO DE SOJA, 2.; MERCOSOJA 2002, 2002, Foz do Iguaçu. Perspectivas do agronegócio da soja: resumos. Londrina: Embrapa Soja, 2002. p. 42. (Embrapa Soja. Documentos, 181). Organizado por Odilon Ferreira Saraiva, Clara Beatriz Hoffmann-Campo. Biblioteca(s): Embrapa Soja. |
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58. | | ALMEIDA, A. M. R.; VLIET, H. van der; KITAJIMA, E. W.; PIUGA, F. F.; MARIN, S. R. R.; VALENTININ, N.; BINNECK, E.; NEPOMUCENO, A. L.; BENATO, L. C. Necrose da haste da soja associada a um carlavirus. Fitopatologia Brasileira, Brasília, v. 27, p. S199, ago. 2002. Suplemento. Resumo 666. Edição do XXXV Congresso Brasileiro de Fitopatologia, Recife, PE, ago. 2002. Biblioteca(s): Embrapa Soja. |
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59. | | ALMEIDA, A. M. R.; MARIN, S. R. R.; VALENTIM, N.; BITTNECK, E.; NEPOMUCENO, A. L.; BENATO, L. C.; VLIET, H. V. D.; KITAJIMA, E. W.; PIUGA, F. F. Necrose da haste: uma nova virose da soja no Brasil. Londrina: Embrapa Soja, 2002. 11 p. (Embrapa Soja. Circular Técnica, 36). Biblioteca(s): Embrapa Rondônia. |
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60. | | ALMEIDA, A. M. R.; MARIN, S. R. R.; VALENTIN, N.; BITTNECK, E.; NEPOMUCENO, A. L.; BENATO, L. C.; VAN DER VLET, H.; KITAJIMA, E. W.; PIUGA, F. F. Necrose da haste: uma nova virose da soja no Brasil. Londrina: Embrapa Soja, 2002. 11 p. (Embrapa Soja. Circular Técnica, 36) Biblioteca(s): Embrapa Agropecuária Oeste. |
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Registros recuperados : 225 | |
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Registro Completo
Biblioteca(s): |
Embrapa Soja. |
Data corrente: |
25/03/2013 |
Data da última atualização: |
26/03/2013 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
GIROTTO, L.; SOLDERA, M. C. A.; MARIN, S. R. R.; KANAMORI, N.; FUJITA, Y.; YOSHIDA, T.; MARCELINO-GUIMARÃES, F. C.; YAMAGUCHI-SHINOZAKI; NEPOMUCENO, A. L. |
Afiliação: |
SILVANA REGINA ROCKENBACH MARIN, CNPSO; FRANCISMAR CORREA MARCELINO, CNPSO; ALEXANDRE LIMA NEPOMUCENO, SRI. |
Título: |
Agrobacterium-mediated transformation of soybean variated, BR16, gene 35S:AREB1, analysis by southern blotting. |
Ano de publicação: |
2012 |
Fonte/Imprenta: |
In: INTERNATIONAL CROP SCIENCE CONGRESS, 6., 2012, Bento Gonçalves. [Proceedings...]. [S.l.]: International Crop Science Society, 2012. Poster, 117. 1 CD-ROM. |
Idioma: |
Inglês |
Conteúdo: |
Soybean is the most important source of vegetable oil and protein for human and animal diet in the world. The frequency of occurrence of drought has increased significantly over the last decade, probably associated with climate change occurred due to global warming. Researchers are looking
for a long time to improve and optimize the characteristics of the culture. Several strategies can be used to reduce losses caused by drought, ranging from adequate soil management and agriculture, to the use of irrigation. Another possibility is the development of cultivars adapted to drought
conditions and in this respect, Biotechnology has become an important tool in the development of varieties adapted to different conditions of biotic and abiotic stress. The genetically modified plants can be transformed by direct gene transfer using particle bombardment (biolistics), which inserts a
high number of copies of the transgene into the host genome, resulting many times in deactivation of the transgene by gene silencing. Moreover, the transformed plants via Agrobacterium tumefaciens generally have a low number of copies of the transgene. The use of this method allowed a stable expression and insertion of the interest gene in transgenic plants. The AREB is an important transcription factor signaled by the ABA, which acts in response to regulate the expression of other genes that are involved in tolerance to drought and high salinity stress. This study aimed to analyze the T1 plants transformed Agrobacterium by Southern blotting technique to detect the number of copies of the gene 35S:AREB1. The experiment was conducted at the Laboratory of Plant Biotechnology at Embrapa Soja, Londrina - PR. The process was performed according Kanamori et al., 2011. The Southern blotting was performed on 22 plants T1, AREB positive from two independent events. Of the 22 samples, 20 T1 plants had only one copy number and two plants had two copy numbers. Considering the complexity of the mechanism response to water deficit caused by drought, which can vary depending on the intensity and duration of stress, analysis on the molecular level, physiological and agronomic are needed later to assess the efficiency of the presence of the gene in transformed plants. MenosSoybean is the most important source of vegetable oil and protein for human and animal diet in the world. The frequency of occurrence of drought has increased significantly over the last decade, probably associated with climate change occurred due to global warming. Researchers are looking
for a long time to improve and optimize the characteristics of the culture. Several strategies can be used to reduce losses caused by drought, ranging from adequate soil management and agriculture, to the use of irrigation. Another possibility is the development of cultivars adapted to drought
conditions and in this respect, Biotechnology has become an important tool in the development of varieties adapted to different conditions of biotic and abiotic stress. The genetically modified plants can be transformed by direct gene transfer using particle bombardment (biolistics), which inserts a
high number of copies of the transgene into the host genome, resulting many times in deactivation of the transgene by gene silencing. Moreover, the transformed plants via Agrobacterium tumefaciens generally have a low number of copies of the transgene. The use of this method allowed a stable expression and insertion of the interest gene in transgenic plants. The AREB is an important transcription factor signaled by the ABA, which acts in response to regulate the expression of other genes that are involved in tolerance to drought and high salinity stress. This study aimed to analyze the T1 plants transform... Mostrar Tudo |
Thesagro: |
Biotecnologia; Soja. |
Thesaurus NAL: |
Biotechnology; Soybeans. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 03123naa a2200265 a 4500 001 1954079 005 2013-03-26 008 2012 bl uuuu u00u1 u #d 100 1 $aGIROTTO, L. 245 $aAgrobacterium-mediated transformation of soybean variated, BR16, gene 35S$bAREB1, analysis by southern blotting. 260 $c2012 520 $aSoybean is the most important source of vegetable oil and protein for human and animal diet in the world. The frequency of occurrence of drought has increased significantly over the last decade, probably associated with climate change occurred due to global warming. Researchers are looking for a long time to improve and optimize the characteristics of the culture. Several strategies can be used to reduce losses caused by drought, ranging from adequate soil management and agriculture, to the use of irrigation. Another possibility is the development of cultivars adapted to drought conditions and in this respect, Biotechnology has become an important tool in the development of varieties adapted to different conditions of biotic and abiotic stress. The genetically modified plants can be transformed by direct gene transfer using particle bombardment (biolistics), which inserts a high number of copies of the transgene into the host genome, resulting many times in deactivation of the transgene by gene silencing. Moreover, the transformed plants via Agrobacterium tumefaciens generally have a low number of copies of the transgene. The use of this method allowed a stable expression and insertion of the interest gene in transgenic plants. The AREB is an important transcription factor signaled by the ABA, which acts in response to regulate the expression of other genes that are involved in tolerance to drought and high salinity stress. This study aimed to analyze the T1 plants transformed Agrobacterium by Southern blotting technique to detect the number of copies of the gene 35S:AREB1. The experiment was conducted at the Laboratory of Plant Biotechnology at Embrapa Soja, Londrina - PR. The process was performed according Kanamori et al., 2011. The Southern blotting was performed on 22 plants T1, AREB positive from two independent events. Of the 22 samples, 20 T1 plants had only one copy number and two plants had two copy numbers. Considering the complexity of the mechanism response to water deficit caused by drought, which can vary depending on the intensity and duration of stress, analysis on the molecular level, physiological and agronomic are needed later to assess the efficiency of the presence of the gene in transformed plants. 650 $aBiotechnology 650 $aSoybeans 650 $aBiotecnologia 650 $aSoja 700 1 $aSOLDERA, M. C. A. 700 1 $aMARIN, S. R. R. 700 1 $aKANAMORI, N. 700 1 $aFUJITA, Y. 700 1 $aYOSHIDA, T. 700 1 $aMARCELINO-GUIMARÃES, F. C. 700 1 $aYAMAGUCHI-SHINOZAKI 700 1 $aNEPOMUCENO, A. L. 773 $tIn: INTERNATIONAL CROP SCIENCE CONGRESS, 6., 2012, Bento Gonçalves. [Proceedings...]. [S.l.]: International Crop Science Society, 2012. Poster, 117. 1 CD-ROM.
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