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Biblioteca(s): |
Embrapa Rondônia. |
Data corrente: |
09/11/2017 |
Data da última atualização: |
09/11/2017 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
AZÊVEDO, H. F.; BENVINDO, F. D.; CAVALVCANTE, L. N.; HAVERROTH, M.; WADT, L. H. de O.; CAMPOS, T. de. |
Afiliação: |
LUCIA HELENA DE OLIVEIRA WADT, CPAF-Rondonia. |
Título: |
Transferability of heterologous microsatellite loci between species of Euterpe genus. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Genetics and Molecular Research, Ribeirão Preto v. 16, n. 4, p. 1-7, 2017. |
ISSN: |
1676-5680 |
Idioma: |
Português |
Conteúdo: |
Euterpe precatoria, popularly known as "açaí-do-amazonas", has agronomic, technological and economic potential and has been gaining prominence with the commercialization of its fruits. The production of this açaí-do-amazonas is based on extractivism and management practices have been recommended for sustainable collection. To recommend sustainable rates of fruit collection, it is essential to have information on the genetic variability of natural populations to monitor the recruitment and population dynamics. The objective of this study was to evaluate the transferability of microsatellite loci of E. edulis species to E. precatoria to confirm the applicability of these markers in genetic studies. Eighteen microsatellite loci developed for E. edulis were analyzed using 20 individuals from two natural populations (Formoso and Novo Segredo) of E. precatoria collected at Feijó, State of Acre, Brazil. All loci (100%) were amplified, and of these, nine (50%) were polymorphic. A total of 29 alleles were found in the Novo Segredo population, and 27 alleles were found in the Formoso population, ranging from two to five alleles per locus, with a mean of three alleles per locus. The expected heterozygosity in the Formoso population varied from 0.100 to 0.668, with an average of 0.421. In the Novo Segredo population, a variation between 0.100 and 0.710, with a mean of 0.418, was found. The observed heterozygosity values ranged from 0.100 to 0.800, with averages of 0.333 and 0.267 for Formoso and Novo Segredo, respectively. This set of markers will support further studies on the molecular characterization of the natural populations of E. precatoria and assist with the recommendation of sustainable management practices and strategies for the conservation and genetic improvement of this species. MenosEuterpe precatoria, popularly known as "açaí-do-amazonas", has agronomic, technological and economic potential and has been gaining prominence with the commercialization of its fruits. The production of this açaí-do-amazonas is based on extractivism and management practices have been recommended for sustainable collection. To recommend sustainable rates of fruit collection, it is essential to have information on the genetic variability of natural populations to monitor the recruitment and population dynamics. The objective of this study was to evaluate the transferability of microsatellite loci of E. edulis species to E. precatoria to confirm the applicability of these markers in genetic studies. Eighteen microsatellite loci developed for E. edulis were analyzed using 20 individuals from two natural populations (Formoso and Novo Segredo) of E. precatoria collected at Feijó, State of Acre, Brazil. All loci (100%) were amplified, and of these, nine (50%) were polymorphic. A total of 29 alleles were found in the Novo Segredo population, and 27 alleles were found in the Formoso population, ranging from two to five alleles per locus, with a mean of three alleles per locus. The expected heterozygosity in the Formoso population varied from 0.100 to 0.668, with an average of 0.421. In the Novo Segredo population, a variation between 0.100 and 0.710, with a mean of 0.418, was found. The observed heterozygosity values ranged from 0.100 to 0.800, with averages of 0.333 and 0.267 for Fo... Mostrar Tudo |
Palavras-Chave: |
Acre; Amazônia Ocidental; Dinámica poblacional; Ensayos de variedades; Feijó (AC); Fitomejoramiento; Formoso; Marcador microssatélite; Novo Segredo; Repeticiones de microsatélite; Transferability; Transferibilidad; Transferibilidade; Variación genética; Western Amazon. |
Thesagro: |
Açaí; Dinâmica populacional; Marcador molecular; Melhoramento genético vegetal; Variação Genética; Variedade. |
Thesaurus Nal: |
Euterpe precatoria; genetic variation; loci; microsatellite repeats; plant breeding; population dynamics; variety trials. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/166466/1/26407.pdf
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Marc: |
LEADER 03349naa a2200529 a 4500 001 2079402 005 2017-11-09 008 2017 bl --- 0-- u #d 022 $a1676-5680 100 1 $aAZÊVEDO, H. F. 245 $aTransferability of heterologous microsatellite loci between species of Euterpe genus.$h[electronic resource] 260 $c2017 520 $aEuterpe precatoria, popularly known as "açaí-do-amazonas", has agronomic, technological and economic potential and has been gaining prominence with the commercialization of its fruits. The production of this açaí-do-amazonas is based on extractivism and management practices have been recommended for sustainable collection. To recommend sustainable rates of fruit collection, it is essential to have information on the genetic variability of natural populations to monitor the recruitment and population dynamics. The objective of this study was to evaluate the transferability of microsatellite loci of E. edulis species to E. precatoria to confirm the applicability of these markers in genetic studies. Eighteen microsatellite loci developed for E. edulis were analyzed using 20 individuals from two natural populations (Formoso and Novo Segredo) of E. precatoria collected at Feijó, State of Acre, Brazil. All loci (100%) were amplified, and of these, nine (50%) were polymorphic. A total of 29 alleles were found in the Novo Segredo population, and 27 alleles were found in the Formoso population, ranging from two to five alleles per locus, with a mean of three alleles per locus. The expected heterozygosity in the Formoso population varied from 0.100 to 0.668, with an average of 0.421. In the Novo Segredo population, a variation between 0.100 and 0.710, with a mean of 0.418, was found. The observed heterozygosity values ranged from 0.100 to 0.800, with averages of 0.333 and 0.267 for Formoso and Novo Segredo, respectively. This set of markers will support further studies on the molecular characterization of the natural populations of E. precatoria and assist with the recommendation of sustainable management practices and strategies for the conservation and genetic improvement of this species. 650 $aEuterpe precatoria 650 $agenetic variation 650 $aloci 650 $amicrosatellite repeats 650 $aplant breeding 650 $apopulation dynamics 650 $avariety trials 650 $aAçaí 650 $aDinâmica populacional 650 $aMarcador molecular 650 $aMelhoramento genético vegetal 650 $aVariação Genética 650 $aVariedade 653 $aAcre 653 $aAmazônia Ocidental 653 $aDinámica poblacional 653 $aEnsayos de variedades 653 $aFeijó (AC) 653 $aFitomejoramiento 653 $aFormoso 653 $aMarcador microssatélite 653 $aNovo Segredo 653 $aRepeticiones de microsatélite 653 $aTransferability 653 $aTransferibilidad 653 $aTransferibilidade 653 $aVariación genética 653 $aWestern Amazon 700 1 $aBENVINDO, F. D. 700 1 $aCAVALVCANTE, L. N. 700 1 $aHAVERROTH, M. 700 1 $aWADT, L. H. de O. 700 1 $aCAMPOS, T. de 773 $tGenetics and Molecular Research, Ribeirão Preto$gv. 16, n. 4, p. 1-7, 2017.
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Embrapa Rondônia (CPAF-RO) |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
10/08/2021 |
Data da última atualização: |
10/08/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
SGUIZZATO, A. L. L.; MARCONDES, M. I.; VALADARES FILHO, S. C.; CATON, J.; NEVILLE, T. L.; MACHADO, F. S.; PACHECO, M. V. C.; ROTTA, P. P. |
Afiliação: |
ANNA L. L. SGUIZZATO, Universidade Federal de Viçosa; MARCOS I. MARCONDES, Universidade Federal de Viçosa; SEBASTIÃO C. VALADARES FILHO, Universidade Federal de Viçosa; JOEL CATON, North Dakota State University; TAMMI L. NEVILLE, North Dakota State University; FERNANDA SAMARINI MACHADO, CNPGL; MARCOS V. C. PACHECO, Universidade Federal de Viçosa; POLYANA P. ROTTA, Universidade Federal de Viçosa. |
Título: |
Body composition changes of crossbred Holstein × Gyr cows and conceptus during pregnancy. |
Ano de publicação: |
2020 |
Fonte/Imprenta: |
Journal of Dairy Science, v. 103, n. 3, p. 2773-2783, 2020. |
DOI: |
https://doi.org/10.3168/jds.2019-17490 |
Idioma: |
Inglês |
Conteúdo: |
This study aimed to evaluate the effects of plane of nutrition and advancing days of pregnancy (DP) on maternal body composition and fetal development. Differing planes of nutrition were established by 2 feeding regimens (FR): ad libitum (AL) or maintenance (MA). Sixty-two nonlactating multiparous Holstein × Gyr cows with average body weight of 480 ± 10.1 kg and an age of 5 ± 0.5 yr were used. Cows were divided into 3 groups: pregnant (n = 44), nonpregnant (n = 12), and baseline reference cows (n = 6). The 56 pregnant and nonpregnant cows were randomly allocated into 2 different FR: AL or MA. Cows fed at MA received 1.15% of their body weight on a dry matter (DM) basis, receiving corn silage and a concentrate-based diet at a ratio of 93:7 on a DM basis. Reference group cows were slaughtered at the beginning of the experimental period to estimate body composition and empty body weight. To evaluate the effects of DP, pregnant and nonpregnant animals were slaughtered at d 140, 200, 240, and 270 of gestation. Feeding regimen affected maternal tissue composition. Days of pregnancy affected fresh weight (FW), DM, and energy content, but no differences were observed for crude protein (CP) and ether extract (EE) because of DP. Feeding regimen affected mammary gland components (CP, EE, and energy content), but not fresh or dry weights. Days of pregnancy influenced almost all mammary gland components except energy content. Regarding the uterus, FR affected only fresh and dry weights; however, DP affected every uterus component measured. The only interaction between FR and DP in this study was observed for placental FW. Cows fed AL on d 270 presented the same placental FW as cows at MA and AL on d 200 and 240. Further, pregnant cows fed at MA on d 270 had greater placental FW than cows fed AL at this day. Days of pregnancy, but not FR, influenced the composition of fetal fluids in pregnant cows. Finally, cows fed at MA had greater FW for the fetus than cows fed AL; however, fetus composition changed over DP. The FW, DM, EE, and energy content increased until d 270, but CP decreased. In conclusion, the novelty of our data presents how changes due to FR and DP occur in maternal tissues and the conceptus. MenosThis study aimed to evaluate the effects of plane of nutrition and advancing days of pregnancy (DP) on maternal body composition and fetal development. Differing planes of nutrition were established by 2 feeding regimens (FR): ad libitum (AL) or maintenance (MA). Sixty-two nonlactating multiparous Holstein × Gyr cows with average body weight of 480 ± 10.1 kg and an age of 5 ± 0.5 yr were used. Cows were divided into 3 groups: pregnant (n = 44), nonpregnant (n = 12), and baseline reference cows (n = 6). The 56 pregnant and nonpregnant cows were randomly allocated into 2 different FR: AL or MA. Cows fed at MA received 1.15% of their body weight on a dry matter (DM) basis, receiving corn silage and a concentrate-based diet at a ratio of 93:7 on a DM basis. Reference group cows were slaughtered at the beginning of the experimental period to estimate body composition and empty body weight. To evaluate the effects of DP, pregnant and nonpregnant animals were slaughtered at d 140, 200, 240, and 270 of gestation. Feeding regimen affected maternal tissue composition. Days of pregnancy affected fresh weight (FW), DM, and energy content, but no differences were observed for crude protein (CP) and ether extract (EE) because of DP. Feeding regimen affected mammary gland components (CP, EE, and energy content), but not fresh or dry weights. Days of pregnancy influenced almost all mammary gland components except energy content. Regarding the uterus, FR affected only fresh and dry weights; ... Mostrar Tudo |
Palavras-Chave: |
Composição corporal; Tecido materno. |
Thesagro: |
Bovino; Gado Gir; Gado Holandês; Reprodução Animal; Útero. |
Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
Marc: |
LEADER 03125naa a2200301 a 4500 001 2133414 005 2021-08-10 008 2020 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.3168/jds.2019-17490$2DOI 100 1 $aSGUIZZATO, A. L. L. 245 $aBody composition changes of crossbred Holstein × Gyr cows and conceptus during pregnancy.$h[electronic resource] 260 $c2020 520 $aThis study aimed to evaluate the effects of plane of nutrition and advancing days of pregnancy (DP) on maternal body composition and fetal development. Differing planes of nutrition were established by 2 feeding regimens (FR): ad libitum (AL) or maintenance (MA). Sixty-two nonlactating multiparous Holstein × Gyr cows with average body weight of 480 ± 10.1 kg and an age of 5 ± 0.5 yr were used. Cows were divided into 3 groups: pregnant (n = 44), nonpregnant (n = 12), and baseline reference cows (n = 6). The 56 pregnant and nonpregnant cows were randomly allocated into 2 different FR: AL or MA. Cows fed at MA received 1.15% of their body weight on a dry matter (DM) basis, receiving corn silage and a concentrate-based diet at a ratio of 93:7 on a DM basis. Reference group cows were slaughtered at the beginning of the experimental period to estimate body composition and empty body weight. To evaluate the effects of DP, pregnant and nonpregnant animals were slaughtered at d 140, 200, 240, and 270 of gestation. Feeding regimen affected maternal tissue composition. Days of pregnancy affected fresh weight (FW), DM, and energy content, but no differences were observed for crude protein (CP) and ether extract (EE) because of DP. Feeding regimen affected mammary gland components (CP, EE, and energy content), but not fresh or dry weights. Days of pregnancy influenced almost all mammary gland components except energy content. Regarding the uterus, FR affected only fresh and dry weights; however, DP affected every uterus component measured. The only interaction between FR and DP in this study was observed for placental FW. Cows fed AL on d 270 presented the same placental FW as cows at MA and AL on d 200 and 240. Further, pregnant cows fed at MA on d 270 had greater placental FW than cows fed AL at this day. Days of pregnancy, but not FR, influenced the composition of fetal fluids in pregnant cows. Finally, cows fed at MA had greater FW for the fetus than cows fed AL; however, fetus composition changed over DP. The FW, DM, EE, and energy content increased until d 270, but CP decreased. In conclusion, the novelty of our data presents how changes due to FR and DP occur in maternal tissues and the conceptus. 650 $aBovino 650 $aGado Gir 650 $aGado Holandês 650 $aReprodução Animal 650 $aÚtero 653 $aComposição corporal 653 $aTecido materno 700 1 $aMARCONDES, M. I. 700 1 $aVALADARES FILHO, S. C. 700 1 $aCATON, J. 700 1 $aNEVILLE, T. L. 700 1 $aMACHADO, F. S. 700 1 $aPACHECO, M. V. C. 700 1 $aROTTA, P. P. 773 $tJournal of Dairy Science$gv. 103, n. 3, p. 2773-2783, 2020.
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