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 | Acesso ao texto completo restrito à biblioteca da Embrapa Suínos e Aves. Para informações adicionais entre em contato com cnpsa.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
22/11/2019 |
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Data da última atualização: |
10/03/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
VIANA, C.; SERENO, M. J.; BERSOT, L. dos S.; KICH, J. D.; NERO, L. A. |
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Afiliação: |
CIBELI VIANA, UFV; MALLU JAGNOW SERENO, UFPR; LUCIANO DOS SANTOS BERSOT, UFPR; JALUSA DEON KICH, CNPSA; LUIS AUGUSTO NERO, UFV. |
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Título: |
Comparison of meat juice serology and bacteriology for surveillance of salmonella in the Brazilian pork production chain. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Foodborne Pathogens and Disease, v. 17, n. 3, p. 194-201, 2020. |
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DOI: |
10.1089/fpd.2019.2712 |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract: This study assessed an enzyme-linked immunosorbent assay (ELISA) based assay to detect Salmonella in swine as a potential tool to predict the presence of Salmonella in swine carcasses. The following samples were collected from 10 swine batches: blood (n = 100); environment (barn floor, n = 10, and lairage floor, n = 10); meat juice (n = 100, obtained after defrosting of diaphragm); tonsils (n = 100); mesenteric lymph nodes (MLNs) (n = 100); and carcasses after bleeding (n = 100), after singeing (n = 100), after evisceration (n = 100), and after final rinsing (n = 100). Blood and meat juice were subjected to ELISA to detect antibodies against Salmonella, and other samples were subjected to Salmonella detection by ISO 6579. Salmonella was detected in 3 samples from barn floors, 7 lairage floors, 45 tonsils, 43 MLNs and in 3 carcasses. Based on ELISA, Salmonella positive samples were: 86 and 46 blood serum (20% and 40% cut-offs) and 68 and 46 meat juice (20% and 40% cut-offs). Optical density readings from blood serum and meat juice presented a high and significant correlation (r = 0.93, p < 0.001), and a substantial agreement for Salmonella detection (K = 0.69, ELISA 40% cut-off). The agreement between ELISA and microbiological analysis for Salmonella detection in pig carcasses were absent or poor, with the exception of results obtained by ELISA 40% cut-off from blood serum and meat juice with MLNs (K = 0.49 and 0.50, respectively) and tonsils (K = 0.29 and 0.30, respectively). Based on the obtained results, meat juice can be considered an alternative to blood serum as a matrix for ELISA for preliminary detection of Salmonella, allowing the identification of potential sources of contamination during slaughtering. MenosAbstract: This study assessed an enzyme-linked immunosorbent assay (ELISA) based assay to detect Salmonella in swine as a potential tool to predict the presence of Salmonella in swine carcasses. The following samples were collected from 10 swine batches: blood (n = 100); environment (barn floor, n = 10, and lairage floor, n = 10); meat juice (n = 100, obtained after defrosting of diaphragm); tonsils (n = 100); mesenteric lymph nodes (MLNs) (n = 100); and carcasses after bleeding (n = 100), after singeing (n = 100), after evisceration (n = 100), and after final rinsing (n = 100). Blood and meat juice were subjected to ELISA to detect antibodies against Salmonella, and other samples were subjected to Salmonella detection by ISO 6579. Salmonella was detected in 3 samples from barn floors, 7 lairage floors, 45 tonsils, 43 MLNs and in 3 carcasses. Based on ELISA, Salmonella positive samples were: 86 and 46 blood serum (20% and 40% cut-offs) and 68 and 46 meat juice (20% and 40% cut-offs). Optical density readings from blood serum and meat juice presented a high and significant correlation (r = 0.93, p < 0.001), and a substantial agreement for Salmonella detection (K = 0.69, ELISA 40% cut-off). The agreement between ELISA and microbiological analysis for Salmonella detection in pig carcasses were absent or poor, with the except... Mostrar Tudo |
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Thesagro: |
Bacteriologia; Cadeia Produtiva; Carne; Elisa; Enzima; Salmonella; Suíno; Técnica Imunoenzimática. |
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Thesaurus Nal: |
Bacteriology; Disease surveillance; Enzyme-linked immunosorbent assay; Isolation techniques; Pork. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02918naa a2200337 a 4500
001 2114901
005 2020-03-10
008 2020 bl uuuu u00u1 u #d
024 7 $a10.1089/fpd.2019.2712$2DOI
100 1 $aVIANA, C.
245 $aComparison of meat juice serology and bacteriology for surveillance of salmonella in the Brazilian pork production chain.$h[electronic resource]
260 $c2020
520 $aAbstract: This study assessed an enzyme-linked immunosorbent assay (ELISA) based assay to detect Salmonella in swine as a potential tool to predict the presence of Salmonella in swine carcasses. The following samples were collected from 10 swine batches: blood (n = 100); environment (barn floor, n = 10, and lairage floor, n = 10); meat juice (n = 100, obtained after defrosting of diaphragm); tonsils (n = 100); mesenteric lymph nodes (MLNs) (n = 100); and carcasses after bleeding (n = 100), after singeing (n = 100), after evisceration (n = 100), and after final rinsing (n = 100). Blood and meat juice were subjected to ELISA to detect antibodies against Salmonella, and other samples were subjected to Salmonella detection by ISO 6579. Salmonella was detected in 3 samples from barn floors, 7 lairage floors, 45 tonsils, 43 MLNs and in 3 carcasses. Based on ELISA, Salmonella positive samples were: 86 and 46 blood serum (20% and 40% cut-offs) and 68 and 46 meat juice (20% and 40% cut-offs). Optical density readings from blood serum and meat juice presented a high and significant correlation (r = 0.93, p < 0.001), and a substantial agreement for Salmonella detection (K = 0.69, ELISA 40% cut-off). The agreement between ELISA and microbiological analysis for Salmonella detection in pig carcasses were absent or poor, with the exception of results obtained by ELISA 40% cut-off from blood serum and meat juice with MLNs (K = 0.49 and 0.50, respectively) and tonsils (K = 0.29 and 0.30, respectively). Based on the obtained results, meat juice can be considered an alternative to blood serum as a matrix for ELISA for preliminary detection of Salmonella, allowing the identification of potential sources of contamination during slaughtering.
650 $aBacteriology
650 $aDisease surveillance
650 $aEnzyme-linked immunosorbent assay
650 $aIsolation techniques
650 $aPork
650 $aBacteriologia
650 $aCadeia Produtiva
650 $aCarne
650 $aElisa
650 $aEnzima
650 $aSalmonella
650 $aSuíno
650 $aTécnica Imunoenzimática
700 1 $aSERENO, M. J.
700 1 $aBERSOT, L. dos S.
700 1 $aKICH, J. D.
700 1 $aNERO, L. A.
773 $tFoodborne Pathogens and Disease$gv. 17, n. 3, p. 194-201, 2020.
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Registro original: |
Embrapa Suínos e Aves (CNPSA) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Agropecuária Oeste; Embrapa Arroz e Feijão; Embrapa Milho e Sorgo; Embrapa Tabuleiros Costeiros. |
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Data corrente: |
27/08/2012 |
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Data da última atualização: |
27/08/2012 |
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Tipo da produção científica: |
Comunicado Técnico/Recomendações Técnicas |
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Autoria: |
MELO, L. C.; COSTA, J. G. C. da; PEREIRA, H. S.; DEL PELOSO, M. J.; WENDLAND, A.; FARIA, L. C. de; NASCENTE, A. S.; CABRERA DIAZ, J. L.; CARVALHO, H. W. L. de; COSTA, A. F. da; ALMEIDA, V. M. de; MELO, C. L. P. de; MAGALDI, M. C. de S.; ABREU, A. de F. B.; MOREIRA, J. A. A.; PEREIRA FILHO, I. A.; CARGNIN, A.; POSSE, S. C. P.; SOUZA FILHO, B. F. de; MOURA NETO, F. P.; SOUZA, J. F.; GUIMARÃES, C. M.; BRAZ, A. J. B. P.; FERREIRA, S. B.; MARANGON, M. A.; SOUZA, N. P. de; OLIVEIRA, J. P. de; FARIA, J. C. de; BASSINELLO, P. Z.; VIANELLO, R. P. |
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Afiliação: |
LEONARDO CUNHA MELO, CNPAF; JOAQUIM GERALDO CAPRIO DA COSTA, CNPAF; HELTON SANTOS PEREIRA, CNPAF; MARIA JOSE DEL PELOSO, CNPAF; ADRIANE WENDLAND FERREIRA, CNPAF; LUIS CLAUDIO DE FARIA, CNPAF; ADRIANO STEPHAN NASCENTE, CNPAF; JOSE LUIS CABRERA DIAZ, CNPAF; HELIO WILSON LEMOS DE CARVALHO, CPATC; ANTÔNIO FÉLIX DA COSTA, IPA; VALTER MARTINS DE ALMEIDA, EMPAER-MT; CARLOS LASARO PEREIRA DE MELO, CPAO; MARIANA CRUZICK DE S MAGALDI, CNPAF; ANGELA DE FATIMA BARBOSA ABREU, CNPAF; JOSE ALOISIO ALVES MOREIRA, CNPMS; ISRAEL ALEXANDRE PEREIRA FILHO, CNPMS; ADELIANO CARGNIN, CNPT; SHEILA CRISTINA PRUCOLI POSSE, INCAPER; BENEDITO FERNANDES DE SOUZA FILHO, PESAGRO; FRANCISCO PEREIRA MOURA NETO, CNPAF; JUAREZ FERNANDES SOUZA, FEPAGRO; CLEBER MORAIS GUIMARAES, CNPAF; ANTÔNIO JOAQUIM BRAGA PEREIRA BRAZ, FESURV; SIMONE BORGES FERREIRA, EMATER-GO; MARCOS AURELIO MARANGON, SNTEEN Canoinhas; NILDA PESSOA DE SOUZA, CNPAF; JAISON PEREIRA DE OLIVEIRA, CNPAF; JOSIAS CORREA DE FARIA, CNPAF; PRISCILA ZACZUK BASSINELLO, CNPAF; ROSANA PEREIRA VIANELLO, CNPAF. |
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Título: |
BRS Ametista: cultivar de feijoeiro comum carioca com grãos graúdos e resistência a doenças. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Santo Antônio de Goiás: Embrapa Arroz e Feijão, 2012. |
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Páginas: |
6 p. |
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Série: |
(Embrapa Arroz e Feijão. Comunicado técnico, 206). |
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Idioma: |
Português |
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Conteúdo: |
A BRS Ametista originou-se da hibridação entre as linhagens PR9115957 / LR720982CP realizada na Embrapa Arroz e Feijão, no ano de 1993. Após a obtenção dos cruzamentos, a geração F1 foi semeada em telado para multiplicação também nesse ano. |
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Thesagro: |
Feijão; Melhoramento genético vegetal; Phaseolus vulgaris; Variedade. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/65046/1/comunicadotecnico-206.pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/66566/1/BRS-Ametista.pdf
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Marc: |
LEADER 01793nam a2200529 a 4500
001 1932291
005 2012-08-27
008 2012 bl uuuu u0uu1 u #d
100 1 $aMELO, L. C.
245 $aBRS Ametista$bcultivar de feijoeiro comum carioca com grãos graúdos e resistência a doenças.
260 $aSanto Antônio de Goiás: Embrapa Arroz e Feijão$c2012
300 $a6 p.
490 $a(Embrapa Arroz e Feijão. Comunicado técnico, 206).
520 $aA BRS Ametista originou-se da hibridação entre as linhagens PR9115957 / LR720982CP realizada na Embrapa Arroz e Feijão, no ano de 1993. Após a obtenção dos cruzamentos, a geração F1 foi semeada em telado para multiplicação também nesse ano.
650 $aFeijão
650 $aMelhoramento genético vegetal
650 $aPhaseolus vulgaris
650 $aVariedade
700 1 $aCOSTA, J. G. C. da
700 1 $aPEREIRA, H. S.
700 1 $aDEL PELOSO, M. J.
700 1 $aWENDLAND, A.
700 1 $aFARIA, L. C. de
700 1 $aNASCENTE, A. S.
700 1 $aCABRERA DIAZ, J. L.
700 1 $aCARVALHO, H. W. L. de
700 1 $aCOSTA, A. F. da
700 1 $aALMEIDA, V. M. de
700 1 $aMELO, C. L. P. de
700 1 $aMAGALDI, M. C. de S.
700 1 $aABREU, A. de F. B.
700 1 $aMOREIRA, J. A. A.
700 1 $aPEREIRA FILHO, I. A.
700 1 $aCARGNIN, A.
700 1 $aPOSSE, S. C. P.
700 1 $aSOUZA FILHO, B. F. de
700 1 $aMOURA NETO, F. P.
700 1 $aSOUZA, J. F.
700 1 $aGUIMARÃES, C. M.
700 1 $aBRAZ, A. J. B. P.
700 1 $aFERREIRA, S. B.
700 1 $aMARANGON, M. A.
700 1 $aSOUZA, N. P. de
700 1 $aOLIVEIRA, J. P. de
700 1 $aFARIA, J. C. de
700 1 $aBASSINELLO, P. Z.
700 1 $aVIANELLO, R. P.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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