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Registro Completo |
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Biblioteca(s): |
Embrapa Cerrados; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
11/12/2020 |
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Data da última atualização: |
11/12/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
MARQUES, T. C.; SANTOS, E. C. da S.; DIESEL, T. O.; MARTINS, C. F.; CUMPA, H. C. B.; LEME, L. de O.; DODE, M. A. N.; ALVES, B. G.; COSTA, F. P. H.; OLIVEIRA, E. B.; GAMBARINI, M. L. |
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Afiliação: |
CARLOS FREDERICO MARTINS, CPAC; MARGOT ALVES NUNES DODE, Cenargen. |
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Título: |
Blastocoel fluid removal and melatonin supplementation in the culture medium improve the viability of vitrified bovine embryos. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Theriogenology, Vv. 160, p. 134-141, 2021. |
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Páginas: |
p. 134-141 |
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ISSN: |
0093-691X |
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Idioma: |
Português |
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Conteúdo: |
In this study, we investigated the effects of melatonin supplementation in the culture medium and blastocoel fluid removal (BFR) before vitrification on the quality and viability of in vitro-derived bovine embryos. After fertilization, presumptive zygotes were assigned to one of the following treatments: control, in vitro standard culture (IVC) medium; IVC þ M109 , IVC medium supplemented 109 M melatonin; or IVC þ M109 BFR, IVC medium supplemented with 109 M melatonin plus BFR on day 7 (D7) of culture. D7 blastocysts were vitrified by the Cryotop method and, after 5 mo of storage, were warmed and incubated for an additional 72 h. The re-expansion rate was evaluated after 2 and 24 h, and the hatching rate was evaluated after 24, 48, and 72 h. At 72 h, the total number of cells (TNC); number of apoptotic cells (NAC); and expression of genes related to oxidative stress (HSPA5), cell metabolism (SLC2A3), cell repair (MSH6), placentation (KRT8 and PLAC8), and implantation (FOSL1) were assessed in the blastocysts. Less than 30% of the control blastocysts re-expanded until 2 h, whereas more than 85% of the IVC þ M109 and IVC þ M109 BFR blastocysts re-expanded (P < 0.05). The hatching rate of IVC þ M109 BFR blastocysts increased at all time points (P < 0.05), reaching 66.8% at 72 h of incubation. The TNC was similar among treatments (P > 0.05), regardless of vitrification/warming and re-cultivation. The NAC:TNC was smaller for melatonin-treated blastocysts (P < 0.05). BFR increased HSPA5 (P ¼ 0.0118) expression and did not affect SLC2A3, MSH6, KRT8, and FOSL1 expression (P > 0.05). In conclusion, melatonin (109 M) supplementation in the culture medium and BFR on D7 of culture increased the hatching rate 24, 48, and 72 h after warming of the vitrified embryos, indicating an improvement in cryotolerance. MenosIn this study, we investigated the effects of melatonin supplementation in the culture medium and blastocoel fluid removal (BFR) before vitrification on the quality and viability of in vitro-derived bovine embryos. After fertilization, presumptive zygotes were assigned to one of the following treatments: control, in vitro standard culture (IVC) medium; IVC þ M109 , IVC medium supplemented 109 M melatonin; or IVC þ M109 BFR, IVC medium supplemented with 109 M melatonin plus BFR on day 7 (D7) of culture. D7 blastocysts were vitrified by the Cryotop method and, after 5 mo of storage, were warmed and incubated for an additional 72 h. The re-expansion rate was evaluated after 2 and 24 h, and the hatching rate was evaluated after 24, 48, and 72 h. At 72 h, the total number of cells (TNC); number of apoptotic cells (NAC); and expression of genes related to oxidative stress (HSPA5), cell metabolism (SLC2A3), cell repair (MSH6), placentation (KRT8 and PLAC8), and implantation (FOSL1) were assessed in the blastocysts. Less than 30% of the control blastocysts re-expanded until 2 h, whereas more than 85% of the IVC þ M109 and IVC þ M109 BFR blastocysts re-expanded (P < 0.05). The hatching rate of IVC þ M109 BFR blastocysts increased at all time points (P < 0.05), reaching 66.8% at 72 h of incubation. The TNC was similar among treatments (P > 0.05), regardless of vitrification/warming and re-cultivation. The NAC:TNC was smaller for melatonin-treated blastocysts (P < 0.05). BFR increased ... Mostrar Tudo |
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Palavras-Chave: |
Apoptose celular; Criotolerância; Fertilização; Melatonina; Vitrificação. |
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Thesagro: |
Cultura de Embrião; Embrião. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02843naa a2200349 a 4500
001 2127986
005 2020-12-11
008 2021 bl uuuu u00u1 u #d
022 $a0093-691X
100 1 $aMARQUES, T. C.
245 $aBlastocoel fluid removal and melatonin supplementation in the culture medium improve the viability of vitrified bovine embryos.$h[electronic resource]
260 $c2021
300 $ap. 134-141
520 $aIn this study, we investigated the effects of melatonin supplementation in the culture medium and blastocoel fluid removal (BFR) before vitrification on the quality and viability of in vitro-derived bovine embryos. After fertilization, presumptive zygotes were assigned to one of the following treatments: control, in vitro standard culture (IVC) medium; IVC þ M109 , IVC medium supplemented 109 M melatonin; or IVC þ M109 BFR, IVC medium supplemented with 109 M melatonin plus BFR on day 7 (D7) of culture. D7 blastocysts were vitrified by the Cryotop method and, after 5 mo of storage, were warmed and incubated for an additional 72 h. The re-expansion rate was evaluated after 2 and 24 h, and the hatching rate was evaluated after 24, 48, and 72 h. At 72 h, the total number of cells (TNC); number of apoptotic cells (NAC); and expression of genes related to oxidative stress (HSPA5), cell metabolism (SLC2A3), cell repair (MSH6), placentation (KRT8 and PLAC8), and implantation (FOSL1) were assessed in the blastocysts. Less than 30% of the control blastocysts re-expanded until 2 h, whereas more than 85% of the IVC þ M109 and IVC þ M109 BFR blastocysts re-expanded (P < 0.05). The hatching rate of IVC þ M109 BFR blastocysts increased at all time points (P < 0.05), reaching 66.8% at 72 h of incubation. The TNC was similar among treatments (P > 0.05), regardless of vitrification/warming and re-cultivation. The NAC:TNC was smaller for melatonin-treated blastocysts (P < 0.05). BFR increased HSPA5 (P ¼ 0.0118) expression and did not affect SLC2A3, MSH6, KRT8, and FOSL1 expression (P > 0.05). In conclusion, melatonin (109 M) supplementation in the culture medium and BFR on D7 of culture increased the hatching rate 24, 48, and 72 h after warming of the vitrified embryos, indicating an improvement in cryotolerance.
650 $aCultura de Embrião
650 $aEmbrião
653 $aApoptose celular
653 $aCriotolerância
653 $aFertilização
653 $aMelatonina
653 $aVitrificação
700 1 $aSANTOS, E. C. da S.
700 1 $aDIESEL, T. O.
700 1 $aMARTINS, C. F.
700 1 $aCUMPA, H. C. B.
700 1 $aLEME, L. de O.
700 1 $aDODE, M. A. N.
700 1 $aALVES, B. G.
700 1 $aCOSTA, F. P. H.
700 1 $aOLIVEIRA, E. B.
700 1 $aGAMBARINI, M. L.
773 $tTheriogenology, Vv. 160, p. 134-141, 2021.
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Embrapa Cerrados (CPAC) |
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Biblioteca(s): |
Embrapa Suínos e Aves. |
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Data corrente: |
01/03/2012 |
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Data da última atualização: |
01/03/2012 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
MANZKE, N. E.; ATHAYDE, N. B.; DALLA COSTA, O. A.; LIMA, G. J. M. M. de. |
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Afiliação: |
NAIANA EINHARDT MANZKE, UFPEL; NATALIA BORTOLETO ATHAYDE, UNESP; OSMAR ANTONIO DALLA COSTA, CNPSA; GUSTAVO JULIO MELLO M DE LIMA, CNPSA. |
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Título: |
Novos desenvolvimentos na nutrição dos leitões nas fases de crescimento e terminação. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: SIMPÓSIO INTERNACIONAL DE SUINOCULTURA, 6., Porto Alegre, RS. Produção, reprodução e sanidade suína: anais Porto Alegre: UFRGS, 2011. p. 43-62. |
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Idioma: |
Português |
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Notas: |
Projeto/Plano de Ação: 03.07.05.019. |
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Thesagro: |
Crescimento; Leitão; Nutrição. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/54918/1/novos-desenvolvimentos-na-nutricao.pdf
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Marc: |
LEADER 00695nam a2200181 a 4500
001 1917212
005 2012-03-01
008 2011 bl uuuu u00u1 u #d
100 1 $aMANZKE, N. E.
245 $aNovos desenvolvimentos na nutrição dos leitões nas fases de crescimento e terminação.$h[electronic resource]
260 $aIn: SIMPÓSIO INTERNACIONAL DE SUINOCULTURA, 6., Porto Alegre, RS. Produção, reprodução e sanidade suína: anais Porto Alegre: UFRGS, 2011. p. 43-62.$c2011
500 $aProjeto/Plano de Ação: 03.07.05.019.
650 $aCrescimento
650 $aLeitão
650 $aNutrição
700 1 $aATHAYDE, N. B.
700 1 $aDALLA COSTA, O. A.
700 1 $aLIMA, G. J. M. M. de
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