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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos; Embrapa Meio-Norte. |
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Data corrente: |
16/12/2019 |
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Data da última atualização: |
16/12/2019 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NEGREIROS, A. B.; SILVA, G. R.; OLIVEIRA, F. A. S.; RESENDE, H. C.; FERNANDES-SALOMÃO, T. M.; MAGGIONI, R.; PEREIRA, F. de M.; SOUZA, B. de A.; LOPES, M. T. do R.; DINIZ, F. M. |
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Afiliação: |
ALINE B. NEGREIROS, UFPI; GEICE R. SILVA, UFPI; FRANCISCA A. S. OLIVEIRA, UFC; HELDER C. RESENDE, UFV; TÂNIA M. FERNANDES-SALOMÃO, UFV; RODRIGO MAGGIONI, UFC; FABIA DE MELLO PEREIRA, CPAMN; BRUNO DE ALMEIDA SOUZA, CPAMN; MARIA TERESA DO REGO LOPES, CPAMN; FABIO MENDONCA DINIZ, CNPC. |
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Título: |
Microsatellite marker discovery in the stingless bee Uruçu-Amarela (Melipona rufiventris Group, Hymenoptera, Meliponini) for population genetic analysis. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Insects, v. 10, n. 12, article 450, Dec. 2019. |
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DOI: |
https://doi.org/10.3390/insects10120450 |
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Idioma: |
Inglês |
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Conteúdo: |
The species Melipona rufiventris Lepeletier, 1836 is a Brazilian native stingless bee that is part of a species complex known as the 'rufiventris group', making it diffcult to distinguish between the different species. Populations in this group are facing a severe decline, leading to the risk of local extinction, and therefore, their conservation should be treated as a major concern. |
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Palavras-Chave: |
Diferenciação genética; Genetic differentiation; Genética populacional; NGS technology. |
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Thesagro: |
Conservação; Marcador Molecular. |
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Thesaurus Nal: |
Population genetics. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/207193/1/Microsatellite-Marker-Discovery-in-the-Stingless-Bee-Melipona-rufiventris-1.pdf
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Marc: |
LEADER 01446naa a2200325 a 4500
001 2117005
005 2019-12-16
008 2019 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.3390/insects10120450$2DOI
100 1 $aNEGREIROS, A. B.
245 $aMicrosatellite marker discovery in the stingless bee Uruçu-Amarela (Melipona rufiventris Group, Hymenoptera, Meliponini) for population genetic analysis.$h[electronic resource]
260 $c2019
520 $aThe species Melipona rufiventris Lepeletier, 1836 is a Brazilian native stingless bee that is part of a species complex known as the 'rufiventris group', making it diffcult to distinguish between the different species. Populations in this group are facing a severe decline, leading to the risk of local extinction, and therefore, their conservation should be treated as a major concern.
650 $aPopulation genetics
650 $aConservação
650 $aMarcador Molecular
653 $aDiferenciação genética
653 $aGenetic differentiation
653 $aGenética populacional
653 $aNGS technology
700 1 $aSILVA, G. R.
700 1 $aOLIVEIRA, F. A. S.
700 1 $aRESENDE, H. C.
700 1 $aFERNANDES-SALOMÃO, T. M.
700 1 $aMAGGIONI, R.
700 1 $aPEREIRA, F. de M.
700 1 $aSOUZA, B. de A.
700 1 $aLOPES, M. T. do R.
700 1 $aDINIZ, F. M.
773 $tInsects$gv. 10, n. 12, article 450, Dec. 2019.
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Registro original: |
Embrapa Meio-Norte (CPAMN) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Instrumentação. Para informações adicionais entre em contato com cnpdia.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Instrumentação. |
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Data corrente: |
23/03/2022 |
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Data da última atualização: |
24/01/2024 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
COUTINHO, T. C.; MALAFATTI, J. O. D.; PARIS, E. C.; TARDIOLI, P. W.; FARINAS, C. S. |
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Afiliação: |
ELAINE CRISTINA PARIS, CNPDIA; CRISTIANE SANCHEZ FARINAS, CNPDIA. |
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Título: |
Hydroxyapatite-CoFe2O4 magnetic nanoparticle composites for industrial enzyme immobilization, use, and recovery. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
ACS Applied Nano Materials, v. 3, 2020. |
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Páginas: |
12334–12345 |
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DOI: |
https://dx.doi.org/10.1021/acsanm.0c02811 |
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Idioma: |
Inglês |
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Conteúdo: |
Hydroxyapatite (HA) is an inorganic material with high ability to interact with proteins and has been recently explored as a support for enzymeimmobilization. However, there are still some drawbacks concerning the recovery of these biocatalysts, which could be overcome using magnetic supports. Cobalt ferrite(CoFe2O4) is a purely magnetic material, which offers excellent chemical stability, ease of synthesis, and mechanical hardness, being a promising candidate to form composites with HA and produce magnetic HA nanoparticles. Therefore, investigation of synthesis procedures and applications of HA/CoFe2O4 composites to facilitate enzyme recovery by means of a magnetic field could find interest in a broad spectrum of biotechnological processes. Here, the co-precipitation method was used to synthesize HA/CoFe2O4 composites with different HA/CoFe2O4 mass ratios, for application as supports for enzyme immobilization. The enzymes β-glucosidase, phytase, and xylanase were selected for proof of concept due to their wide range of industrial applications. The composite with the highest cobalt ferrite content (2:1 mass ratio) was highly effective for immobilization of the three different enzymes, with immobilization yields (IYs) of 70−100% and recovered activities of 78−100%. The biocatalysts could be easily recovered from the reaction media by both centrifugation and application of an external magnetic field,demonstrating their potential for use in industrial processes. The materials exhibited good reusability, especially in the case of the βglucosidase biocatalyst, which could be reused 10 times, maintaining MenosHydroxyapatite (HA) is an inorganic material with high ability to interact with proteins and has been recently explored as a support for enzymeimmobilization. However, there are still some drawbacks concerning the recovery of these biocatalysts, which could be overcome using magnetic supports. Cobalt ferrite(CoFe2O4) is a purely magnetic material, which offers excellent chemical stability, ease of synthesis, and mechanical hardness, being a promising candidate to form composites with HA and produce magnetic HA nanoparticles. Therefore, investigation of synthesis procedures and applications of HA/CoFe2O4 composites to facilitate enzyme recovery by means of a magnetic field could find interest in a broad spectrum of biotechnological processes. Here, the co-precipitation method was used to synthesize HA/CoFe2O4 composites with different HA/CoFe2O4 mass ratios, for application as supports for enzyme immobilization. The enzymes β-glucosidase, phytase, and xylanase were selected for proof of concept due to their wide range of industrial applications. The composite with the highest cobalt ferrite content (2:1 mass ratio) was highly effective for immobilization of the three different enzymes, with immobilization yields (IYs) of 70−100% and recovered activities of 78−100%. The biocatalysts could be easily recovered from the reaction media by both centrifugation and application of an external magnetic field,demonstrating their potential for use in industrial processes... Mostrar Tudo |
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Palavras-Chave: |
Cobalt ferrite; Immobilization; Magnetic nanoparticles. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02369naa a2200229 a 4500
001 2141192
005 2024-01-24
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://dx.doi.org/10.1021/acsanm.0c02811$2DOI
100 1 $aCOUTINHO, T. C.
245 $aHydroxyapatite-CoFe2O4 magnetic nanoparticle composites for industrial enzyme immobilization, use, and recovery.$h[electronic resource]
260 $c2022
300 $a12334–12345
520 $aHydroxyapatite (HA) is an inorganic material with high ability to interact with proteins and has been recently explored as a support for enzymeimmobilization. However, there are still some drawbacks concerning the recovery of these biocatalysts, which could be overcome using magnetic supports. Cobalt ferrite(CoFe2O4) is a purely magnetic material, which offers excellent chemical stability, ease of synthesis, and mechanical hardness, being a promising candidate to form composites with HA and produce magnetic HA nanoparticles. Therefore, investigation of synthesis procedures and applications of HA/CoFe2O4 composites to facilitate enzyme recovery by means of a magnetic field could find interest in a broad spectrum of biotechnological processes. Here, the co-precipitation method was used to synthesize HA/CoFe2O4 composites with different HA/CoFe2O4 mass ratios, for application as supports for enzyme immobilization. The enzymes β-glucosidase, phytase, and xylanase were selected for proof of concept due to their wide range of industrial applications. The composite with the highest cobalt ferrite content (2:1 mass ratio) was highly effective for immobilization of the three different enzymes, with immobilization yields (IYs) of 70−100% and recovered activities of 78−100%. The biocatalysts could be easily recovered from the reaction media by both centrifugation and application of an external magnetic field,demonstrating their potential for use in industrial processes. The materials exhibited good reusability, especially in the case of the βglucosidase biocatalyst, which could be reused 10 times, maintaining
653 $aCobalt ferrite
653 $aImmobilization
653 $aMagnetic nanoparticles
700 1 $aMALAFATTI, J. O. D.
700 1 $aPARIS, E. C.
700 1 $aTARDIOLI, P. W.
700 1 $aFARINAS, C. S.
773 $tACS Applied Nano Materials$gv. 3, 2020.
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Embrapa Instrumentação (CNPDIA) |
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