| |
|
|
| Registros recuperados : 220 | |
| 101. |  | MADRUGA, C. R.; SCHENK, M. A. M.; KESSLER, R. H.; MIGUITA, M. Desenvolvimento de um teste de imunoadsorcao enzimatica (ELISA) para deteccao de anticorpos contra Babesia bigemina. In : SEMINARIO BRASILEIRO DE PARASITOLOGIA VETERINARIA, 10.; SEMINARIO DE PARASITOLOGIA VETERINARIA DO MERCOSUL, 1., 1997, Itajai. Anais... Revista Brasileira de Parasitologia Veterinaria, Sao Paulo, v.6, n.2, p. 304, ago. 1997. Suplemento 1. Resumo A7. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
|   |
| 103. | | ARAUJO, F. R.; MADRUGA, C. R.; LEAL, C. R. B.; MASSUDA, T.; SCHENK, M. A. M. Desenvolvimento de uma prova de imunoadsorcao enzimatica (ELISA) para deteccao de anticorpos contra Trypanosoma vivax. Resultados preliminares. In : SEMINARIO BRASILEIRO DE PARASITOLOGIA VETERINARIA, 10.; SEMINARIO DE PARASITOLOGIA VETERINARIA DO MERCOSUL, 1., 1997, Itajai. Anais... Revista Brasileira de Parasitologia Veterinaria, Sao Paulo, v.6, n.2, p. 350, ago. 1997. Suplemento 1. Resumo P53. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 104. | | MADRUGA, C. R.; KESSLER, R. H.; SCHENK, M. A. M.; GOMES, A.; FIGUEIREDO, G. R. de. Epidemiologia de Anaplasma marginale e Babesia sp. em bezerros das racas Ibage, Nelore e cruzamentos na regiao de Campo Grande, MS. In: CONGRESSO BRASILEIRO DE MEDICINA VETERINARIA, 18.; CONGRESSO INTERNACIONAL DE VETERINARIA EM LINGUA PORTUGUESA, 3.; SEMINARIO BRASILEIRO DE PARASITOLOGIA VETERINARIA, 3, 1982, Balneario Camboriu. Resumos. Florianopolis : SBMV / SOMEVESC, 1982. p.197. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 106. | | MARANA, E. R. M.; VIDOTTO, O.; MADRUGA, C. R.; ANDRADE, G. M.; ALFIERI, A. A. Evaluation of a card agglutination test (CA), IFA, ELISA and cELISA detection of antibodies against Anaplasma marginale. In: CONGRESO PANAMERICANO DE CIENCIAS VETERINARIAS, 16., 1998, Santa Cruz de la Sierra. Memorias. Santa Cruz de la Sierra, Asociacion Panamericana de Ciencias Veterinarias, 1998. p.183. TL. c53. CNPGC. PANVET, 16.| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 107. | | KESSLER, R. H.; MADRUGA, C. R.; JESUS, E. F. de; SEMPREBOM, D. V. Isolamento de cepas puras de Babesia bovis, Babesia bigemina e Anaplasma marginale em area enzootica. Pesquisa Agropecuaria Brasileira, Brasilia, v.22, n.7, p.747-752, jul.1987.| Biblioteca(s): Embrapa Gado de Corte; Embrapa Unidades Centrais. |
|  |
| 110. | | ARAUJO, F. R.; LEAL, C. R. B.; MADRUGA, C. R.; MIGUITA, M.; CARVALHO, E. L. L. Levantamento sorologico para Anaplasma marginale em duas microrregioes do Estado da Bahia. In: SEMINARIO BRASILEIRO DE PRASITOLOGIA VETERINARIA, 9., 1995, Campo Grande. Anais. Revista Brasileira de Parasitologia Veterinaria, Sao Paulo, v.4, n.2, p.188, ago., 1995. Suplemento, 1. CNPGC. Resumo| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 111. | | MENK, J. C. F.; ALMEIDA, M. A. O.; MADRUGA, C. R.; ARAUJO, F. R. Levantamento sorologico da Babesia bovis e Babesia bigemina, no semi-arido baiano, pela tecnica de imunoadsorcao enzimatica (ELISA). In: SEMINARIO BRASILEIRO DE PARASITOLOGIA VETERINARIA, 11., SEMINARIO DE PARASITOLOGIA VETERINARIA DOS PAISES DO MERCOSUL, 2; SIMPOSIO DE CONTROLE INTEGRADO DE PARASITOS DE BOVINOS, 1., 1999, Salvador. Anais... Ilheus: Colegio Brasileiro de Parasitolgia Veterinaria, [1999?]. p.205. CNPGC. Resumo TL-PB-461.| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 112. | | MADRUGA, C. R.; MAJIWA, P.; SILVA, R. A. M.; LEAL, C. R. B.; MORZARIA, J. Genetic characterization of Trypanosoma vivax and Trypanosoma evansi isolated in the Pantanal Region of Brazil. In: CONGRESO PANAMERICANO DE CIENCIAS VETERINARIAS, 16., 1998, Santa Cruz de la Sierra. Memorias. Santa Cruz de la Sierra, Asociacion Panamericana de Ciencias Veterinarias, 1998. p.205. TL. b64. CNPGC. PANVET, 16.| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 113. | | MADRUGA, C. R.; HONER, M. R.; ANDREOTTI, R.; ARAUJO, F. R. de; SANTAREM, V. Simulacao e sorologia no mapeamento da instabilidade enzootica das Babesiose : um estudo nas regioes do Boqueirao e Cariri, Estado da Paraiba. In: SEMINARIO BRASILEIRO DE PARASITOLOGIA VETERINARIA, 8., 1993, Londrina. Anais. [S.l.] : CBPV, [1993?]. P1. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 114. | | GÓES-CAVALCANTE; G.; PFEIFER, I. B.; MADRUGA, C. R.; OLIVEIRA, D. M. C. Seroepidemiology of bovine tripanosamiasis by Trypanosoma vivax in the State of Pará. Revista Brasileira de Parasitologia Veterinária, São Paulo, v.13, p. 233, 2004. Suplemento 1, ref. 107. Edição anais do XIII Congresso Brasileiro de Parasitologia Veterinária e I Simpósio Latino-Americano de Rickettsioses, Ouro Preto, MG, set. 2004. CNPGC.| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 116. | | SANTOS, H. Q.; MADRUGA, C. R.; MARQUES, A. P. C.; QUEIROZ, R. A. Prevalence of Anaplasma marginale in dairy herds of Goiania microregion by indirect fluoroscent antibody technique and enzyme linked immunosorbent assay. In: CONGRESO PANAMERICANO DE CIENCIAS VETERINARIAS, 16., 1998, Santa Cruz de la Sierra. Memorias. Santa Cruz de la Sierra, Asociacion Panamericana de Ciencias Veterinarias, 1998. p.204. TL. d93. CNPGC. PANVET, 16.| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 118. | | ARAUJO, F. R.; MADRUGA, C. R.; MIGUITA, M.; LEAL, C. R. B.; CARVALHO, E. L. L. Prevalencia de anticorpos contra Babesia bovis e Babesia bigemina em bovinos no Estado da Bahia. In: SEMINARIO BRASILEIRO DE PRASITOLOGIA VETERINARIA, 9., 1995, Campo Grande. Anais. Revista Brasileira de Parasitologia Veterinaria, Sao Paulo, v.4, n.2, p.187, ago., 1995. Suplemento, 1. CNPGC. Resumo.| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 119. | | DALMONTE, M. A. S.; RAMOS, J. K. M.; OSORIO, A. L. A. R.; MADRUGA, C. R. Prevalencia da Babesia spp em equinos no Estado de Mato Grosso do Sul e Uruguay por imunofluorescencia indireta. In: SEMINARIO BRASILEIRO DE PARASITOLOGIA VETERINARIA, 11.; SEMINARIO DE PARASITOLOGIA VETERINARIA DOS PAISES DO MERCOSUL, 2.; SIMPOSIO DE CONTROLE INTEGRADO DE PARASITOS DE BOVINOS, 1., 1999, Salvador. Anais... Ilheus: Colegio Brasileiro de Parasitologia Veterinaria, [1999?]. p.205. CNPGC. Resumo-TL-PB-460.| Biblioteca(s): Embrapa Gado de Corte. |
| |
| Registros recuperados : 220 | |
|
|
|
 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Corte. Para informações adicionais entre em contato com cnpgc.biblioteca@embrapa.br. |
Registro Completo
|
Biblioteca(s): |
Embrapa Gado de Corte. |
|
Data corrente: |
25/03/2003 |
|
Data da última atualização: |
25/03/2003 |
|
Autoria: |
MADRUGA, C. R.; LEAL, C. R. B.; FERREIRA, A. M. T.; ARAÚJO, F. R.; BONATO, A. L. V.; KESSLER, R. H.; SCHENK, M. A. M.; SOARES, C. O. |
|
Afiliação: |
Embrapa Gado de Corte (Campo Grande, MS). |
|
Título: |
Genetic and antigenic analysis of Babesia bigemina isolates from five geographical regios of Brazil. |
|
Ano de publicação: |
2002 |
|
Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 22, n. 4, p. 153-160, out./dez. 2002. |
|
Idioma: |
Inglês |
|
Notas: |
CNPGC. |
|
Conteúdo: |
A molecular epidemiological study was performed with Babesia bigemina isolates from five geographical regions of Brazil. The genetic analysis was done with random amplification of polymorphic DNA (RAPD), repetitive extragenic palindromic elements-polymerase chain reaction (REP-PCR) and enterobacterial repetitive intergenic consensus sequences-polymerase chain reaction (ERIC-PCR) that showed genetic polymorphism between these isolates and generated fingerprinting. In RAPD, ILO872 and ILO876 primers were able to detect at least one fingerprinting for each B. bigemina isolate. The amplification of B. bigemina DNA fragments by REP-PCR and ERIC-PCR gave evidence for the presence in this haemoprotozoan of the sequences described previously in microorganisms of the bacterial kingdom. For the first time it was demonstrated that both techniques can be used for genetic analysis of a protozoan parasite, although the ERIC-PCR was more discriminatory than REP-PCR. The dendogram with similarity coefficient among isolates showed two clusters and one subcluster. The Northeastern and Mid-Western isolates showed the greatest genetic diversity, while the Southeastern and Southern isolates were the closest. The antigenic analysis was done through indirect fluorescent antibody technique and Western blotting using a panel of monoclonal antibodies directed against epitopes on the merozoite membrane surface, rhoptries and membrane of infected erythrocytes. As expected, the merozoite variable surface antigens, major surface antigen (MSA)-1 and MSA-2 showed antigenic diversity. However, B cell epitopes on rhoptries and infected erythrocytes were conserved among all isolates studied. In this study it was possible to identify variable and conserved antigens, which had already been described as potential immunogens. Considering that an attenuated Babesia clone used as immunogen selected populations capable of evading the immunity induced by this vaccine, it is necessary to evaluate more deeply the cross-protection conferred by genetically more distant Brazilian B. bigemina isolates and make an evaluation of the polymorphism degree of variable antigens such as MSA-1 and MSA-2. MenosA molecular epidemiological study was performed with Babesia bigemina isolates from five geographical regions of Brazil. The genetic analysis was done with random amplification of polymorphic DNA (RAPD), repetitive extragenic palindromic elements-polymerase chain reaction (REP-PCR) and enterobacterial repetitive intergenic consensus sequences-polymerase chain reaction (ERIC-PCR) that showed genetic polymorphism between these isolates and generated fingerprinting. In RAPD, ILO872 and ILO876 primers were able to detect at least one fingerprinting for each B. bigemina isolate. The amplification of B. bigemina DNA fragments by REP-PCR and ERIC-PCR gave evidence for the presence in this haemoprotozoan of the sequences described previously in microorganisms of the bacterial kingdom. For the first time it was demonstrated that both techniques can be used for genetic analysis of a protozoan parasite, although the ERIC-PCR was more discriminatory than REP-PCR. The dendogram with similarity coefficient among isolates showed two clusters and one subcluster. The Northeastern and Mid-Western isolates showed the greatest genetic diversity, while the Southeastern and Southern isolates were the closest. The antigenic analysis was done through indirect fluorescent antibody technique and Western blotting using a panel of monoclonal antibodies directed against epitopes on the merozoite membrane surface, rhoptries and membrane of infected erythrocytes. As expected, the merozoite variable surfac... Mostrar Tudo |
|
Palavras-Chave: |
Antigenic polymorphism; Brasil; Polimorfismo entigenico. |
|
Thesagro: |
Babesia Bigemina; Polimorfismo Genético. |
|
Thesaurus NAL: |
genetic polymorphism. |
|
Categoria do assunto: |
-- |
|
Marc: |
LEADER 03058naa a2200289 a 4500
001 1325152
005 2003-03-25
008 2002 bl uuuu u00u1 u #d
100 1 $aMADRUGA, C. R.
245 $aGenetic and antigenic analysis of Babesia bigemina isolates from five geographical regios of Brazil.
260 $c2002
500 $aCNPGC.
520 $aA molecular epidemiological study was performed with Babesia bigemina isolates from five geographical regions of Brazil. The genetic analysis was done with random amplification of polymorphic DNA (RAPD), repetitive extragenic palindromic elements-polymerase chain reaction (REP-PCR) and enterobacterial repetitive intergenic consensus sequences-polymerase chain reaction (ERIC-PCR) that showed genetic polymorphism between these isolates and generated fingerprinting. In RAPD, ILO872 and ILO876 primers were able to detect at least one fingerprinting for each B. bigemina isolate. The amplification of B. bigemina DNA fragments by REP-PCR and ERIC-PCR gave evidence for the presence in this haemoprotozoan of the sequences described previously in microorganisms of the bacterial kingdom. For the first time it was demonstrated that both techniques can be used for genetic analysis of a protozoan parasite, although the ERIC-PCR was more discriminatory than REP-PCR. The dendogram with similarity coefficient among isolates showed two clusters and one subcluster. The Northeastern and Mid-Western isolates showed the greatest genetic diversity, while the Southeastern and Southern isolates were the closest. The antigenic analysis was done through indirect fluorescent antibody technique and Western blotting using a panel of monoclonal antibodies directed against epitopes on the merozoite membrane surface, rhoptries and membrane of infected erythrocytes. As expected, the merozoite variable surface antigens, major surface antigen (MSA)-1 and MSA-2 showed antigenic diversity. However, B cell epitopes on rhoptries and infected erythrocytes were conserved among all isolates studied. In this study it was possible to identify variable and conserved antigens, which had already been described as potential immunogens. Considering that an attenuated Babesia clone used as immunogen selected populations capable of evading the immunity induced by this vaccine, it is necessary to evaluate more deeply the cross-protection conferred by genetically more distant Brazilian B. bigemina isolates and make an evaluation of the polymorphism degree of variable antigens such as MSA-1 and MSA-2.
650 $agenetic polymorphism
650 $aBabesia Bigemina
650 $aPolimorfismo Genético
653 $aAntigenic polymorphism
653 $aBrasil
653 $aPolimorfismo entigenico
700 1 $aLEAL, C. R. B.
700 1 $aFERREIRA, A. M. T.
700 1 $aARAÚJO, F. R.
700 1 $aBONATO, A. L. V.
700 1 $aKESSLER, R. H.
700 1 $aSCHENK, M. A. M.
700 1 $aSOARES, C. O.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 22, n. 4, p. 153-160, out./dez. 2002.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Corte (CNPGC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
