Data/hora: 19/06/2024 11:48:12

Biblioteca(s): | Embrapa Arroz e Feijão.
Data corrente: | 27/01/2023
Data da última atualização: | 24/03/2023
Autoria: | MACHADO-ROSA, T. A.; BARBOSA, E. T.; CÔRTES, M. V. de C. B.; LOBO JUNIOR, M.
Afiliação: | TARIANE A. MACHADO-ROSA, UNIVERSIDADE FEDERAL DE GOIÁS; ELDER TADEU BARBOSA, CNPAF; MARCIO VINICIUS DE C BARROS CORTES, CNPAF; MURILLO LOBO JUNIOR, CNPAF.
Título: | Microtiter method for quantitative assay of IAA from fungal isolates, demonstrated with Trichoderma.
Ano de publicação: | 2023
Fonte/Imprenta: | Rhizosphere, v. 25, 100666, 2023.
ISSN: | 2452-2198
DOI: | https://doi.org/10.1016/j.rhisph.2023.100666
Idioma: | Inglês
Conteúdo: | A quantitative and accurate method is needed to estimate indole-3-acetic acid (IAA) production by Trichoderma to compare strains and allow the repeatability of experiments in plant-growth promotion studies. Suspensions of 1.0 × 106 conidia. mL-1 of 98 Brazilian strains were grown in potato + dextrose broth in the absence or presence of L-tryptophan (100 mg L-1) in 1 mL microtubes. After 12,000 rpm spinning for 15 min, 150 &#956;L of the super-natant were added to 100 &#956;L of Salkowski reagent with 30 min incubation at room temperature in the dark, with IAA yield spectrophotometrically estimated at 540 nm. Isolates BRM-29474 and BRM-29574 that outperformed in the IAA production increased tomato root and shoot mass and number of root tips in a greenhouse test, endorsing their potential for plant growth promotion. This adjustment allows a quantitative method to estimate an IAA production by Trichoderma spp., which is more informative than the standard qualitative method.
Palavras-Chave: | Biocontrol; Plant growth promotion; Stepwise screening.
Thesagro: | Estimulante de Crescimento Vegetal; Fungo; Trichoderma.
Thesaurus Nal: | Auxins; Plant growth; Root exudates.
Categoria do assunto: | X Pesquisa, Tecnologia e Engenharia
Registro original: | Embrapa Arroz e Feijão (CNPAF)
Exemplares: | CNPAF - (36628-1) - UPC - AP - DD - 2023 -  - 2023 - DIS