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Registro Completo |
Biblioteca(s): |
Embrapa Gado de Leite. |
Data corrente: |
17/04/2019 |
Data da última atualização: |
24/01/2023 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SABINO, Y. N. V.; FOCHAT, R. C.; LIMA, J. C. F.; RIBEIRO, M. T.; ARCURI, P. B.; CARNEIRO, J. da C.; MACHADO, M. A.; REIS, D. R. de L.; MACHADO, A. B. F.; HÚNGARO, H. M.; RIBEIRO, J. B.; PAIVA, A. D. |
Afiliação: |
Yasmin Neves Vieira Sabino, UFJF; Romário Costa Fochat, UFJF; JUNIOR CESAR FERNANDES LIMA, CNPGL; MARLICE TEIXEIRA RIBEIRO, CNPGL; PEDRO BRAGA ARCURI, CNPGL; JAILTON DA COSTA CARNEIRO, CNPGL; MARCO ANTONIO MACHADO, CNPGL; DANIELE RIBEIRO DE LIMA REIS FAZA, CNPGL; Alessandra Barbosa Ferreira Machado, UFTM, Uberaba; Humberto Moreira Húngaro, UFJF; JOAO BATISTA RIBEIRO, CNPGL; Aline Dias Paiva, UFTM, Uberaba. |
Título: |
Antibacterial activity and lantibiotic post-translational modification genes in Streptococcus spp. isolated from ruminal fluid. |
Ano de publicação: |
2019 |
Fonte/Imprenta: |
Annals of Microbiology, v. 69, p. 131-138, 2019. |
DOI: |
10.1007/s13213-018-1407-2 |
Idioma: |
Inglês |
Conteúdo: |
Abstract The production of bacteriocins is frequently described in high microbial diversity environments. The aims of this study were to screen Streptococcus spp. isolated from rumen for their antibacterial potential and to determine the presence of post-translational modification genes for lantibiotic class of bacteriocins. The isolates were tested for production of antibacterial compounds by the spot-on-lawn assay. Presence of interfering factors and the sensitivity to proteinase K were evaluated. The ruminal bacteria were identified by 16S rRNA gene sequencing and the subspecific discrimination of the isolates belonging to the same specie was performed by PFGE. The presence of lantibiotic post-translational modification genes (lanB, lanC, and lanM) into bacterial genomes was performed by PCR. The bacteriocin-like inhibitory substances showed broad inhibitory activity and the producer cells were identified as S. equinus, S. lutetiensis, and S. gallolyticus. According to PFGE, the isolates identified as S. equinus belong to different strains. Three ruminal isolates showed at least one of the lantibiotic post-translational modification genes, and lanC was more frequently detected (75%). The production of broad-spectrum bacteriocin-like inhibitory substances by rumen strains suggests that antimicrobial peptides may play an important role in competition in the complex ruminal ecosystem. |
Palavras-Chave: |
Antibacterial activity; Bacteriocin; LanC; Lantibiotic; Streptococcus sp. |
Thesagro: |
Rúmen. |
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
Marc: |
LEADER 02399naa a2200337 a 4500 001 2108297 005 2023-01-24 008 2019 bl uuuu u00u1 u #d 024 7 $a10.1007/s13213-018-1407-2$2DOI 100 1 $aSABINO, Y. N. V. 245 $aAntibacterial activity and lantibiotic post-translational modification genes in Streptococcus spp. isolated from ruminal fluid.$h[electronic resource] 260 $c2019 520 $aAbstract The production of bacteriocins is frequently described in high microbial diversity environments. The aims of this study were to screen Streptococcus spp. isolated from rumen for their antibacterial potential and to determine the presence of post-translational modification genes for lantibiotic class of bacteriocins. The isolates were tested for production of antibacterial compounds by the spot-on-lawn assay. Presence of interfering factors and the sensitivity to proteinase K were evaluated. The ruminal bacteria were identified by 16S rRNA gene sequencing and the subspecific discrimination of the isolates belonging to the same specie was performed by PFGE. The presence of lantibiotic post-translational modification genes (lanB, lanC, and lanM) into bacterial genomes was performed by PCR. The bacteriocin-like inhibitory substances showed broad inhibitory activity and the producer cells were identified as S. equinus, S. lutetiensis, and S. gallolyticus. According to PFGE, the isolates identified as S. equinus belong to different strains. Three ruminal isolates showed at least one of the lantibiotic post-translational modification genes, and lanC was more frequently detected (75%). The production of broad-spectrum bacteriocin-like inhibitory substances by rumen strains suggests that antimicrobial peptides may play an important role in competition in the complex ruminal ecosystem. 650 $aRúmen 653 $aAntibacterial activity 653 $aBacteriocin 653 $aLanC 653 $aLantibiotic 653 $aStreptococcus sp 700 1 $aFOCHAT, R. C. 700 1 $aLIMA, J. C. F. 700 1 $aRIBEIRO, M. T. 700 1 $aARCURI, P. B. 700 1 $aCARNEIRO, J. da C. 700 1 $aMACHADO, M. A. 700 1 $aREIS, D. R. de L. 700 1 $aMACHADO, A. B. F. 700 1 $aHÚNGARO, H. M. 700 1 $aRIBEIRO, J. B. 700 1 $aPAIVA, A. D. 773 $tAnnals of Microbiology$gv. 69, p. 131-138, 2019.
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Embrapa Gado de Leite (CNPGL) |
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
Data corrente: |
02/06/2023 |
Data da última atualização: |
18/09/2023 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
ROCHA, L. B. da; FREIRE, G. da S.; MACHADO, C. de A.; SILVA, A. V. C. da; LEDO, A. da S. |
Afiliação: |
LETICIA BISPO DA ROCHA, UFS; GILMARA DA SILVA FREIRE, UFS; CAROLINE DE ARAUJO MACHADO, SEDUC; ANA VERUSKA CRUZ DA SILVA MUNIZ, CPATC; ANA DA SILVA LEDO, CPATC. |
Título: |
Viabilidade de grãos de pólen de mangabeira à temperatura ambiente. |
Ano de publicação: |
2022 |
Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 7., 2022. Multifuncionalidade e Qualidade de Vida: [anais...]. Brasília, DF: Sociedade Brasileira de Recursos Genéticos, 2022. |
Idioma: |
Português |
Palavras-Chave: |
Erosão genética. |
Thesagro: |
Fruta Tropical; Hancornia Speciosa; Mangaba; Pólen. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1154193/1/Viabilidade-de-graos-de-polen.2022.pdf
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Marc: |
LEADER 00737nam a2200205 a 4500 001 2154193 005 2023-09-18 008 2022 bl uuuu u00u1 u #d 100 1 $aROCHA, L. B. da 245 $aViabilidade de grãos de pólen de mangabeira à temperatura ambiente.$h[electronic resource] 260 $aIn: CONGRESSO BRASILEIRO DE RECURSOS GENÉTICOS, 7., 2022. Multifuncionalidade e Qualidade de Vida: [anais...]. Brasília, DF: Sociedade Brasileira de Recursos Genéticos$c2022 650 $aFruta Tropical 650 $aHancornia Speciosa 650 $aMangaba 650 $aPólen 653 $aErosão genética 700 1 $aFREIRE, G. da S. 700 1 $aMACHADO, C. de A. 700 1 $aSILVA, A. V. C. da 700 1 $aLEDO, A. da S.
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