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9. | | PASSAIA, G.; SBEGHEN, F.; MARGIS-PINHEIRO, M.; REVERS, L. F. Avaliação da expressão e identificação de genes diferencialmente regulados durante o desenvolvimento do fruto das cultivares de uva Isabel e Isabel Precoce. In: ENCONTRO DE INICIAÇÃO CIENTÍFICA DA EMBRAPA UVA E VINHO, 5., ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 1., 2007, Bento Gonçalves. Resumos. Bento Gonçalves: Embrapa Uva e Vinho, 2007. p. 47. (Embrapa Uva e Vinho. Documentos, 63). Resumo. Biblioteca(s): Embrapa Uva e Vinho. |
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13. | | FALAVIGNA, V. S.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. Análise funcional e potencial biotecnológico do gene DHN11 de macieira (Malus x domestica Borkh.) In: ENCONTRO DE INICIAÇÃO CIENTÍFICA, 14. ENCONTRO DE PÓS-GRADUANDOS DA EMBRAPA UVA E VINHO, 10., 2016, Bento Gonçalves. Resumos...Bento Gonçalves, RS: Embrapa uva e Vinho, 2016. p. 82. (Embrapa Uva e Vinho. Documentos, 99) Biblioteca(s): Embrapa Uva e Vinho. |
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15. | | PASSAIA, G.; MARGIS-PINHEIRO, M.; FIALHO, F. B.; SBEGHEN, F.; PORTO, D. D.; REVERS, L. F. Characterization of an early berry development grapevine somatic variant (Vitis labrusca L. cv. Isabel Precoce). American Journal of Plant Sciences, v. 5, n. 26, p. 3848-3865, Dec. 2014. Biblioteca(s): Embrapa Uva e Vinho. |
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19. | | TURCHETTO ZOLET, A. C.; CRUZ, F.; VENDRAMIN, G. G.; SIMON, M. F.; SALGUEIRO, F.; MARGIS PINHEIRO, M.; MARGIS, R. Large-scale phylogeography of the disjunct Neotropical tree species Schizolobium parahyba (Fabaceae-Caesalpinioideae). Molecular Phylogenetics and Evolution, v. 65, p. 174-182, 2012. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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20. | | WEBER, R. L. M.; MARGIS-PINHEIRO, M.; GROSSI DE SÁ, M. F.; PASSAGLIA, L.; BODANESE ZANETTINI, M. H. Introdução, via biobalística, de um gene que codifica uma osmotina de Solanum nigrum visando a resistência a moléstias fúngicas em cultivares de soja [Glycine max (L.) MERRIL]. In: CONGRESSO NACIONAL DE BOTÂNICA, 57.; ENCONTRO ESTADUAL DE BOTÂNICOS, 13.; ENCONTRO ESTADUAL DE HERBÁRIOS, 5., 2006, Gramado, RS. [Anais...]. Porto Alegre, RS: Sociedade Botânica do Brasil, 2006. Não paginado. Biblioteca(s): Embrapa Recursos Genéticos e Biotecnologia. |
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Registro Completo
Biblioteca(s): |
Embrapa Uva e Vinho. |
Data corrente: |
27/08/2014 |
Data da última atualização: |
02/04/2019 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
FALAVIGNA, V. da S.; PORTO, D. D.; BUFFON, V.; MARGIS-PINHEIRO, M.; PASQUALI, G.; REVERS, L. F. |
Afiliação: |
Vítor da Silveira Falavigna; Diogo Denardi Porto, CNPUV; Vanessa Buffon, CNPUV; Márcia Margis-Pinheiro; Giancarlo Pasquali; LUIS FERNANDO REVERS, CNPUV. |
Título: |
Differential transcriptional profiles of dormancy-related genes in apple buds |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Plant Molecular Biology Reporter, v. 32, p. 796-813, 2014. |
Idioma: |
Inglês |
Notas: |
DOI 10.1007/s11105-013-0690-0 |
Conteúdo: |
The production of temperate fruit crops depends on plant developmental processes, primarily the shift from the juvenile phase to the reproductive phase, dormancy transitions and flowering. Apple tree (Malus ×domestica Borkh.) development is regulated by chilling temperatures, which are required for bud dormancy progression. The apple cultivar Castel Gala is a spontaneous mutation of "Gala Standard". "Castel Gala" is characterized by a 50 % decrease in the chilling requirement (CR) for dormancy release, which results in an earlier budbreak. This work explores the contrasting phenotypes of these cultivars using suppression subtractive hybridization (SSH). From 1,019 unigenes identified by SSH, we selected 28 candidate genes putatively associated with dormancy cycling. Reverse transcription-quantitative polymerase chain reaction was used to validate the differential expression profiles and to transcriptionally characterize these genes in three distinct apple cultivars ("Castel Gala", "Royal Gala" and "Fuji Standard") during a cycle comprising growth to dormancy. Of the 28 candidate genes analyzed, 17 confirmed the differences in expression predicted by SSH. Seasonal transcript accumulation during the winter was observed for several genes, with higher steady-state mRNA levels maintained longer in cultivars with a high CR. The transcription profiles suggest that these genes may be associated with dormancy establishment and maintenance. Of the 17 candidate genes, transcripts coding for dormancy-associated MADS-box (DAM), dehydrins, GAST1, LTI65, NAC, HTA8, HTA12 and RAP2.12-like proteins displayed major differences in gene expression between cultivars through the winter. These genes were therefore considered good candidates for key roles in the dormancy process in apple trees. MenosThe production of temperate fruit crops depends on plant developmental processes, primarily the shift from the juvenile phase to the reproductive phase, dormancy transitions and flowering. Apple tree (Malus ×domestica Borkh.) development is regulated by chilling temperatures, which are required for bud dormancy progression. The apple cultivar Castel Gala is a spontaneous mutation of "Gala Standard". "Castel Gala" is characterized by a 50 % decrease in the chilling requirement (CR) for dormancy release, which results in an earlier budbreak. This work explores the contrasting phenotypes of these cultivars using suppression subtractive hybridization (SSH). From 1,019 unigenes identified by SSH, we selected 28 candidate genes putatively associated with dormancy cycling. Reverse transcription-quantitative polymerase chain reaction was used to validate the differential expression profiles and to transcriptionally characterize these genes in three distinct apple cultivars ("Castel Gala", "Royal Gala" and "Fuji Standard") during a cycle comprising growth to dormancy. Of the 28 candidate genes analyzed, 17 confirmed the differences in expression predicted by SSH. Seasonal transcript accumulation during the winter was observed for several genes, with higher steady-state mRNA levels maintained longer in cultivars with a high CR. The transcription profiles suggest that these genes may be associated with dormancy establishment and maintenance. Of the 17 candidate genes, transcripts codin... Mostrar Tudo |
Palavras-Chave: |
Apple; Castel Gala; Fuji Standard; Royal Gala. |
Thesagro: |
Dormencia; Genetica vegetal; Maçã; Temperatura. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/107373/1/Falavigna2014-Genes-Apple-Buds.pdf
|
Marc: |
LEADER 02604naa a2200289 a 4500 001 1993551 005 2019-04-02 008 2014 bl uuuu u00u1 u #d 100 1 $aFALAVIGNA, V. da S. 245 $aDifferential transcriptional profiles of dormancy-related genes in apple buds$h[electronic resource] 260 $c2014 500 $aDOI 10.1007/s11105-013-0690-0 520 $aThe production of temperate fruit crops depends on plant developmental processes, primarily the shift from the juvenile phase to the reproductive phase, dormancy transitions and flowering. Apple tree (Malus ×domestica Borkh.) development is regulated by chilling temperatures, which are required for bud dormancy progression. The apple cultivar Castel Gala is a spontaneous mutation of "Gala Standard". "Castel Gala" is characterized by a 50 % decrease in the chilling requirement (CR) for dormancy release, which results in an earlier budbreak. This work explores the contrasting phenotypes of these cultivars using suppression subtractive hybridization (SSH). From 1,019 unigenes identified by SSH, we selected 28 candidate genes putatively associated with dormancy cycling. Reverse transcription-quantitative polymerase chain reaction was used to validate the differential expression profiles and to transcriptionally characterize these genes in three distinct apple cultivars ("Castel Gala", "Royal Gala" and "Fuji Standard") during a cycle comprising growth to dormancy. Of the 28 candidate genes analyzed, 17 confirmed the differences in expression predicted by SSH. Seasonal transcript accumulation during the winter was observed for several genes, with higher steady-state mRNA levels maintained longer in cultivars with a high CR. The transcription profiles suggest that these genes may be associated with dormancy establishment and maintenance. Of the 17 candidate genes, transcripts coding for dormancy-associated MADS-box (DAM), dehydrins, GAST1, LTI65, NAC, HTA8, HTA12 and RAP2.12-like proteins displayed major differences in gene expression between cultivars through the winter. These genes were therefore considered good candidates for key roles in the dormancy process in apple trees. 650 $aDormencia 650 $aGenetica vegetal 650 $aMaçã 650 $aTemperatura 653 $aApple 653 $aCastel Gala 653 $aFuji Standard 653 $aRoyal Gala 700 1 $aPORTO, D. D. 700 1 $aBUFFON, V. 700 1 $aMARGIS-PINHEIRO, M. 700 1 $aPASQUALI, G. 700 1 $aREVERS, L. F. 773 $tPlant Molecular Biology Reporter$gv. 32, p. 796-813, 2014.
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