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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
06/05/2004 |
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Data da última atualização: |
06/05/2004 |
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Autoria: |
TRAVASSOS, C. E.; BENOÎT, C.; VALAS, S.; SILVA, A. G. da; PERRIN, G. |
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Título: |
Caprine arthritis-encephalitis virus in semen of naturally infected bucks. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Small Ruminant Research, v. 32, n. 2, p. 101-106, 1999. |
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Idioma: |
Inglês |
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Conteúdo: |
Semen and peripheral blood mononuclear cells (PBMC) samples of 15 infected and 3 non-infected bucks were evaluated for the presence of caprine arthritis-encephalitis virus (CAEV), by double-nested polymerase chain reaction (d-n-PCR). In order to local the presence of virus, semen samples were separated into cell-free seminal fluids (CFSF), enriched non-spermatozoal cells fraction (NSCF), and spermatozoa (SPZ). All PBMC samples from infected bucks were positive for CAEV-DNA but only 8 out of 15 semen samples were positive for CAEV-DNA. The presence of virus was not necessarily concordant in different fractions of semen from the same buck over time. In addition, the virus was isolated from CFSF in primary goat synovial membrane (GSM) cell culture, in which productive infection was demonstrated by syncytia formation. This is the first report describing the presence of CAEV-DNA and also of replication-competent virus in semen from CAEV seropositive bucks. Such results suggest the possibility of sexual transmission of CAEV. |
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Palavras-Chave: |
Artrite encefalite caprina; CAEV; PCR; Reação de cadeia da polimerase; Transmissão. |
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Thesagro: |
Caprino; Vírus. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01712naa a2200253 a 4500
001 1530454
005 2004-05-06
008 1999 bl uuuu u00u1 u #d
100 1 $aTRAVASSOS, C. E.
245 $aCaprine arthritis-encephalitis virus in semen of naturally infected bucks.
260 $c1999
520 $aSemen and peripheral blood mononuclear cells (PBMC) samples of 15 infected and 3 non-infected bucks were evaluated for the presence of caprine arthritis-encephalitis virus (CAEV), by double-nested polymerase chain reaction (d-n-PCR). In order to local the presence of virus, semen samples were separated into cell-free seminal fluids (CFSF), enriched non-spermatozoal cells fraction (NSCF), and spermatozoa (SPZ). All PBMC samples from infected bucks were positive for CAEV-DNA but only 8 out of 15 semen samples were positive for CAEV-DNA. The presence of virus was not necessarily concordant in different fractions of semen from the same buck over time. In addition, the virus was isolated from CFSF in primary goat synovial membrane (GSM) cell culture, in which productive infection was demonstrated by syncytia formation. This is the first report describing the presence of CAEV-DNA and also of replication-competent virus in semen from CAEV seropositive bucks. Such results suggest the possibility of sexual transmission of CAEV.
650 $aCaprino
650 $aVírus
653 $aArtrite encefalite caprina
653 $aCAEV
653 $aPCR
653 $aReação de cadeia da polimerase
653 $aTransmissão
700 1 $aBENOÎT, C.
700 1 $aVALAS, S.
700 1 $aSILVA, A. G. da
700 1 $aPERRIN, G.
773 $tSmall Ruminant Research$gv. 32, n. 2, p. 101-106, 1999.
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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