| |
|
|
|
Registro Completo |
|
Biblioteca(s): |
Embrapa Meio Ambiente. |
|
Data corrente: |
11/02/2016 |
|
Data da última atualização: |
12/02/2016 |
|
Tipo da produção científica: |
Resumo em Anais de Congresso |
|
Autoria: |
MENDES, L. W.; MENDES, R.; TSAI, S. M. |
|
Afiliação: |
L. W. MENDES, ESALQ/USP; RODRIGO MENDES, CNPMA; S. M. TSAI, ESALQ/USP. |
|
Título: |
Metagenomic analysis of the rhizosphere microbiome of the common bean resistant to Fusarium oxysporum. |
|
Ano de publicação: |
2015 |
|
Fonte/Imprenta: |
In: RHIZOSPHERE, 4., 2015, Maastricht. Stretching the interface of life: abstracts... Maastricht: Wageningen University & Research Centre and the Netherlands Institute of Ecology, 2015. Ref. 56. |
|
Páginas: |
34-35 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The rhizosphere microbiome plays a key role in the functioning of the host plant, influencing its physiology and development. It has been suggested that plants use mechanisms present in the rhizosphere microbiome to fend off infections, such as fungal diseases. This work aimed to assess the microbial community inhabiting the common bean rhizosphere in order to identify potential groups related to the suppression of the soil-borne pathogen Fusarium oxysporum. Therefore, using shotgun metagenomic sequencing (Illumina Miseq), we investigated the phylogenetic and potential functional diversity of microbial communities colonizing the rhizosphere of four cultivars of common bean with different levels of resistance to the fungus, ranging from high susceptibility to resistant. Quantitative PCR of total bacteria in rhizosphere samples showed in increase of 16S rRNA copy number with the increase of resistance to the fungus. Mesocosms experiments, including four common bean cultivars cultivated in Amazonian Dark Earth and three replicates, were conducted in greenhouse conditions and we obtained over than 12 million metagenomic sequences. The overall microbial diversity did not present significant variations across common bean cultivars. From the classified sequences, 97,4% were affiliated to Bacteria and 1,48% to Archaea. Proteobacteria represented the most abundant phyla (41,7%), followed by Actinobacteria (29,4%), Firmicutes (5,9%) and Acidobacteria (4,1%). The microbial communities structure were different between bulk soil and rhizosphere samples. Comparing all bean cultivars, the resistant one showed an overrepresention of the phyla Spirochaetes, Nitrospirae and Euryarchaeota. The resistant bean cultivar presented high number of sequences affiliated to the genus Bacillus. Interestingly, the resistant and moderately resistant cultivars, presented high proportion of sequences related to bacteriocin, a narrow spectrum antibiotics. Preliminary analysis showed that different common bean cultivars could select differential microbial groups in the rhizosphere environment. Further analysis will search for bacterial groups potentially related to the fungal antagonism. MenosThe rhizosphere microbiome plays a key role in the functioning of the host plant, influencing its physiology and development. It has been suggested that plants use mechanisms present in the rhizosphere microbiome to fend off infections, such as fungal diseases. This work aimed to assess the microbial community inhabiting the common bean rhizosphere in order to identify potential groups related to the suppression of the soil-borne pathogen Fusarium oxysporum. Therefore, using shotgun metagenomic sequencing (Illumina Miseq), we investigated the phylogenetic and potential functional diversity of microbial communities colonizing the rhizosphere of four cultivars of common bean with different levels of resistance to the fungus, ranging from high susceptibility to resistant. Quantitative PCR of total bacteria in rhizosphere samples showed in increase of 16S rRNA copy number with the increase of resistance to the fungus. Mesocosms experiments, including four common bean cultivars cultivated in Amazonian Dark Earth and three replicates, were conducted in greenhouse conditions and we obtained over than 12 million metagenomic sequences. The overall microbial diversity did not present significant variations across common bean cultivars. From the classified sequences, 97,4% were affiliated to Bacteria and 1,48% to Archaea. Proteobacteria represented the most abundant phyla (41,7%), followed by Actinobacteria (29,4%), Firmicutes (5,9%) and Acidobacteria (4,1%). The microbial communities ... Mostrar Tudo |
|
Palavras-Chave: |
Metagenômica. |
|
Thesagro: |
Feijão; Fusarium Oxysporum. |
|
Thesaurus Nal: |
Metagenomics. |
|
Categoria do assunto: |
S Ciências Biológicas |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/138807/1/2015RA-067.pdf
|
|
Marc: |
LEADER 02914nam a2200193 a 4500
001 2036658
005 2016-02-12
008 2015 bl uuuu u00u1 u #d
100 1 $aMENDES, L. W.
245 $aMetagenomic analysis of the rhizosphere microbiome of the common bean resistant to Fusarium oxysporum.$h[electronic resource]
260 $aIn: RHIZOSPHERE, 4., 2015, Maastricht. Stretching the interface of life: abstracts... Maastricht: Wageningen University & Research Centre and the Netherlands Institute of Ecology, 2015. Ref. 56.$c2015
300 $a34-35
520 $aThe rhizosphere microbiome plays a key role in the functioning of the host plant, influencing its physiology and development. It has been suggested that plants use mechanisms present in the rhizosphere microbiome to fend off infections, such as fungal diseases. This work aimed to assess the microbial community inhabiting the common bean rhizosphere in order to identify potential groups related to the suppression of the soil-borne pathogen Fusarium oxysporum. Therefore, using shotgun metagenomic sequencing (Illumina Miseq), we investigated the phylogenetic and potential functional diversity of microbial communities colonizing the rhizosphere of four cultivars of common bean with different levels of resistance to the fungus, ranging from high susceptibility to resistant. Quantitative PCR of total bacteria in rhizosphere samples showed in increase of 16S rRNA copy number with the increase of resistance to the fungus. Mesocosms experiments, including four common bean cultivars cultivated in Amazonian Dark Earth and three replicates, were conducted in greenhouse conditions and we obtained over than 12 million metagenomic sequences. The overall microbial diversity did not present significant variations across common bean cultivars. From the classified sequences, 97,4% were affiliated to Bacteria and 1,48% to Archaea. Proteobacteria represented the most abundant phyla (41,7%), followed by Actinobacteria (29,4%), Firmicutes (5,9%) and Acidobacteria (4,1%). The microbial communities structure were different between bulk soil and rhizosphere samples. Comparing all bean cultivars, the resistant one showed an overrepresention of the phyla Spirochaetes, Nitrospirae and Euryarchaeota. The resistant bean cultivar presented high number of sequences affiliated to the genus Bacillus. Interestingly, the resistant and moderately resistant cultivars, presented high proportion of sequences related to bacteriocin, a narrow spectrum antibiotics. Preliminary analysis showed that different common bean cultivars could select differential microbial groups in the rhizosphere environment. Further analysis will search for bacterial groups potentially related to the fungal antagonism.
650 $aMetagenomics
650 $aFeijão
650 $aFusarium Oxysporum
653 $aMetagenômica
700 1 $aMENDES, R.
700 1 $aTSAI, S. M.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Meio Ambiente (CNPMA) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Territorial. |
|
Data corrente: |
23/09/2003 |
|
Data da última atualização: |
30/07/2014 |
|
Tipo da produção científica: |
Artigo em Anais de Congresso |
|
Autoria: |
BATISTELLA, M.; LU, D. |
|
Afiliação: |
Embrapa Monitoramento por Satélite; Indiana University-CIPEC. |
|
Título: |
Integrating field data and remote sensing to identify secondary succession stages in the Amazon. |
|
Ano de publicação: |
2002 |
|
Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM ON REMOTE SENSING OF ENVIRONMENT, 29., 2002, Buenos Aires-Argentina. Anais... Buenos Aires: ISPRS, 2002. 4p. |
|
Descrição Física: |
folhas avulsas, il. |
|
Idioma: |
Inglês |
|
Conteúdo: |
Secondary succession (SS) of tropical forest ecosystems follows disturbances such as deforestation. Sharp distinctions between SS stages are often artificial, but useful in Land-Use/Land-Cover (LULC) classifications. In this paper, results for vegetation structure in Rondônia, Brazilian Amazon, are presented as a basis for discussing the reflectance of SS stages when using Landsat TM imagery. Vegetation structure data were collected through 32 surveys encompassing initial SS (SS1), intermediate SS (SS2), advanced SS (SS3), and forest. The results informed the classification of a TM image acquired in 1998. Statistical analyses were performed. SS1, SS2, SS3, and forest were well separated when using solely the data for vegetation structure (p<0.001). However, analyses of reflectance on selected TM bands allowed the separation of only three of these classes (SS1 and SS2 mixed together, SS3, and forest). These findings contribute to the spatialtemporal monitoring of Amazonian landscapes and their LULC dynamics. |
|
Palavras-Chave: |
Amazonas. |
|
Thesagro: |
Desmatamento; Floresta Tropical Úmida; Sensoriamento Remoto. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/105670/1/849.pdf
|
|
Marc: |
LEADER 01658nam a2200181 a 4500
001 1016844
005 2014-07-30
008 2002 bl uuuu u00u1 u #d
100 1 $aBATISTELLA, M.
245 $aIntegrating field data and remote sensing to identify secondary succession stages in the Amazon.
260 $aIn: INTERNATIONAL SYMPOSIUM ON REMOTE SENSING OF ENVIRONMENT, 29., 2002, Buenos Aires-Argentina. Anais... Buenos Aires: ISPRS, 2002. 4p.$c2002
300 $cfolhas avulsas, il.
520 $aSecondary succession (SS) of tropical forest ecosystems follows disturbances such as deforestation. Sharp distinctions between SS stages are often artificial, but useful in Land-Use/Land-Cover (LULC) classifications. In this paper, results for vegetation structure in Rondônia, Brazilian Amazon, are presented as a basis for discussing the reflectance of SS stages when using Landsat TM imagery. Vegetation structure data were collected through 32 surveys encompassing initial SS (SS1), intermediate SS (SS2), advanced SS (SS3), and forest. The results informed the classification of a TM image acquired in 1998. Statistical analyses were performed. SS1, SS2, SS3, and forest were well separated when using solely the data for vegetation structure (p<0.001). However, analyses of reflectance on selected TM bands allowed the separation of only three of these classes (SS1 and SS2 mixed together, SS3, and forest). These findings contribute to the spatialtemporal monitoring of Amazonian landscapes and their LULC dynamics.
650 $aDesmatamento
650 $aFloresta Tropical Úmida
650 $aSensoriamento Remoto
653 $aAmazonas
700 1 $aLU, D.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Territorial (CNPM) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
