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| Registros recuperados : 16 | |
| 5. |  | BORRÉ, J. G.; RAMALHO, I. O.; HOMEM, B. G. C.; PAES, L N.; MACEDO, R. O.; URQUIAGA, S. Variação na estrutura do dossel de pastagens fertilizadas com N ou consorciadas comMacrotyloma axillare cv. Java ao longo das estações do ano em Seropédica/RJ. In: SEMANA CIENTÍFICA JOHANNA DÖBEREINER, 22., 2022, Seropédica. Bicentenário da Independência: 200 anos de ciência, tecnologia e inovação no Brasil: caderno de resumos... Seropédica: Embrapa Agrobiologia, 2022.| Biblioteca(s): Embrapa Agrobiologia. |
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| 6. | | DEPABLOS, L.; HOMEM, B. G. C.; FERREIRA, I. M.; BERNARDES, T. F.; BODDEY, R. M.; LARA, M. A. S.; CASAGRANDE, D. R. Nitrogen cycling in tropical grass-legume pastures managed under canopy light interception. Nutrient Cycling in Agroecosystems, V. 121,p. 51-67, 2021.| Biblioteca(s): Embrapa Agrobiologia. |
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| 7. | | GOMES, F. K.; OLIVEIRA, M. D. B.; HOMEM, B. G. C.; BODDEY, R. M.; BERNARDES, T. F.; GIONGELLI, M. P.; LARA, M. A. S.; CASAGRANDE, D. R. Effects of grazing management in brachiaria grass-forage peanut pastures on canopy structure and forage intake. Journal of Animal Science, , v. 96, p. 3837-3849, 2018.| Biblioteca(s): Embrapa Agrobiologia. |
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| 9. | | REZENDE, C. de P.; PEREIRA, J. M.; MAGALHÃES, A. B.; HOMEM, B. G. C.; BODDEY, R. M.; ALVES, B. J. R. Desempenho de novilhas em pastagens formadas pelos capins tifton 85, xaraés e mombaça, sob lotação rotativa. Agrotrópica, v. 33, n. 3, p. 197-204, 2021.| Biblioteca(s): Embrapa Agrobiologia. |
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| 11. | | HOMEM, B. G. C.; LIMA, I. B. G. de; SPASIANI, P. P.; FERREIRA, I. G.; BODDEY, R. M.; BERNARDES, T. F.; DUBEUX JUNIOR, J. C. B.; CASAGRANDE, D. R. Palisadegrass pastures with or without nitrogen or mixed with forage peanut grazed to a similar target canopy height. 1. Effects on herbage mass, canopy structure and forage nutritive value. Grass and Forage Science, First published: 06 April 2021.| Biblioteca(s): Embrapa Agrobiologia. |
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| 12. | | HOMEM, B. G. C.; LIMA, I. B. G. de; SPASIANI, P. P.; BORGES, L. P. C.; BODDEY, R. M.; DUBEUX JUNIOR, J. C. B.; BERNARDES, T. F.; CASAGRANDE, D. R. Palisadegrass pastures with or without nitrogen or mixed with forage peanut grazed to a similar target canopy height. 2. Effects on animal performance, forage intake and digestion, and nitrogen metabolism. Grass and Forage Science, v. 76, n. 3, p. 413-426, 2021.| Biblioteca(s): Embrapa Agrobiologia. |
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| 13. | | HOMEM, B. G. C.; LIMA, I. B. G. de; SPASIANI, P. P.; GUIMARÃES, B. C.; GUIMARÃES, G. D.; BERNARDES, T. F.; REZENDE, C. de P.; BODDEY, R. M.; CASAGRANDE, D. R. N-fertiliser application or legume integration enhances N cycling in tropical pastures. Nutrient Cycling in Agroecosystems, Published 02 September 2021.| Biblioteca(s): Embrapa Agrobiologia. |
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| 14. | | GUIMARÃES, B. C.; GOMES, F. de K.; HOMEM, B. G. C.; LIMA, I. B. G. de; SPASIANI, P. S.; BODDEY, R. M.; ALVES, B. J. R.; CASAGRANDE, D. R. Emissions of N2O and NH3 from cattle excreta in grass pastures fertilized with N or mixed with a forage legume. Nutrient Cycling in Agroecosystems, v. 122, p. 325?346, 2022.| Biblioteca(s): Embrapa Agrobiologia. |
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| 15. | | SANTOS, C. A. dos; MONTEIRO, R. C.; HOMEM, B. G. C.; SALGADO, L. S.; CASAGRANDE, D. R.; PEREIRA, J. M.; REZENDE, C. de P; ALVES, B. J. R.; BODDEY, R. M. Productivity of beef cattle grazing Brachiaria brizantha cv. Marandu with and without nitrogen fertilizer application or mixed pastures with the legume Desmodium ovalifolium. Grass and Forage Science, v. 78, n. 1, p. 147-160, 2023.| Biblioteca(s): Embrapa Agrobiologia. |
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| 16. | | SPASIANI, P. P.; HOMEM, B. G. C.; LIMA, I. B. G. de; GUIMARAES, B. C.; MEDEIROS, E. S. de; MUIR, J. P.; OLIVEIRA, M. S. de; BODDEY, R. M.; CASAGRANDEM, D. R. Light competition is the key factor determining spatio-temporal variability in legume proportion within Marandu palisadegrass-forage peanut mixed pastures. Crop & Pasture Science, Published online: 21 March 2023.| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 16 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Recursos Genéticos e Biotecnologia. Para informações adicionais entre em contato com cenargen.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido. |
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Data corrente: |
28/04/2020 |
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Data da última atualização: |
19/11/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
BASSO, M. F.; LOURENCO-TESSUTTI, I. T.; BUSANELLO, C.; PINTO, C. E. M.; FREITAS, E. de O.; RIBEIRO, T. P.; ENGLER, J. de A.; OLIVEIRA, A. C. de; MORGANTE, C. V.; ALVES-FERREIRA, M.; GROSSI-DE-SA, M. F. |
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Afiliação: |
MARCOS FERNANDO BASSO; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; CARLOS BUSANELLO, UFPEL; CLIDIA EDUARDA MOREIRA PINTO, UNB; ELINEA DE OLIVEIRA FREITAS, UNB; JANICE DE ALMEIDA ENGLER, INRA/CNRS/UNS, FRANCE; ANTONIO COSTA DE OLIVEIRA, UFPEL; CAROLINA VIANNA MORGANTE, CPATSA; MARCIO ALVES-FERREIRA, UFRJ; MARIA FATIMA GROSSI DE SA, Cenargen. |
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Título: |
Insights obtained using different modules of the cotton uceA1.7 promoter. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Planta, v. 251, n. 2, 2020. |
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DOI: |
https://doi.org/10.1007/s00425-020-03348-8 |
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Idioma: |
Inglês |
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Conteúdo: |
Transcriptional promoters are among the primary genetic engineering elements used to control genes of interest (GOIs) associated with agronomic traits. Cotton uceA1.7 was previously characterized as a constitutive promoter with activity higher than that of the constitutive promoter from the Cauliflower mosaic virus (CaMV) 35S gene in various plant tissues. In this study, we generated Arabidopsis thaliana homozygous events stably overexpressing the gfp reporter gene driven by different modules of the uceA1.7 promoter. The expression level of the reporter gene in different plant tissues and the transcriptional stability of these modules was determined compared to its full-length promoter and the 35S promoter. The full-length uceA1.7 promoter exhibited higher activity in different plant tissues compared to the 35S promoter. Two modules of the promoter produced a low and unstable transcription level compared to the other promoters. The other two modules rich in cis-regulatory elements showed similar activity levels to full-length uceA1.7 and 35S promoters but were less stable. This result suggests the location of a minimal portion of the promoter that is required to initiate transcription properly (the core promoter). Additionally, the full-length uceA1.7 promoter containing the 5?-untranslated region (UTR) is essential for higher transcriptional stability in various plant tissues. These findings confirm the potential use of the full-length uceA1.7 promoter for the development of new biotechnological tools (NBTs) to achieve higher expression levels of GOIs in, for example, the root or flower bud for the efficient control of phytonematodes and pest-insects, respectively, in important crops. MenosTranscriptional promoters are among the primary genetic engineering elements used to control genes of interest (GOIs) associated with agronomic traits. Cotton uceA1.7 was previously characterized as a constitutive promoter with activity higher than that of the constitutive promoter from the Cauliflower mosaic virus (CaMV) 35S gene in various plant tissues. In this study, we generated Arabidopsis thaliana homozygous events stably overexpressing the gfp reporter gene driven by different modules of the uceA1.7 promoter. The expression level of the reporter gene in different plant tissues and the transcriptional stability of these modules was determined compared to its full-length promoter and the 35S promoter. The full-length uceA1.7 promoter exhibited higher activity in different plant tissues compared to the 35S promoter. Two modules of the promoter produced a low and unstable transcription level compared to the other promoters. The other two modules rich in cis-regulatory elements showed similar activity levels to full-length uceA1.7 and 35S promoters but were less stable. This result suggests the location of a minimal portion of the promoter that is required to initiate transcription properly (the core promoter). Additionally, the full-length uceA1.7 promoter containing the 5?-untranslated region (UTR) is essential for higher transcriptional stability in various plant tissues. These findings confirm the potential use of the full-length uceA1.7 promoter for the development o... Mostrar Tudo |
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Palavras-Chave: |
Cotton constitutive promoter; New biotechnological tools; Transcriptional core promoter; Transgenic crops. |
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Thesagro: |
Algodão; Gene Marcador; Genética. |
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Thesaurus NAL: |
Gene expression. |
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Categoria do assunto: |
--
G Melhoramento Genético |
|
Marc: |
LEADER 02743naa a2200349 a 4500
001 2123510
005 2020-11-19
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s00425-020-03348-8$2DOI
100 1 $aBASSO, M. F.
245 $aInsights obtained using different modules of the cotton uceA1.7 promoter.$h[electronic resource]
260 $c2020
520 $aTranscriptional promoters are among the primary genetic engineering elements used to control genes of interest (GOIs) associated with agronomic traits. Cotton uceA1.7 was previously characterized as a constitutive promoter with activity higher than that of the constitutive promoter from the Cauliflower mosaic virus (CaMV) 35S gene in various plant tissues. In this study, we generated Arabidopsis thaliana homozygous events stably overexpressing the gfp reporter gene driven by different modules of the uceA1.7 promoter. The expression level of the reporter gene in different plant tissues and the transcriptional stability of these modules was determined compared to its full-length promoter and the 35S promoter. The full-length uceA1.7 promoter exhibited higher activity in different plant tissues compared to the 35S promoter. Two modules of the promoter produced a low and unstable transcription level compared to the other promoters. The other two modules rich in cis-regulatory elements showed similar activity levels to full-length uceA1.7 and 35S promoters but were less stable. This result suggests the location of a minimal portion of the promoter that is required to initiate transcription properly (the core promoter). Additionally, the full-length uceA1.7 promoter containing the 5?-untranslated region (UTR) is essential for higher transcriptional stability in various plant tissues. These findings confirm the potential use of the full-length uceA1.7 promoter for the development of new biotechnological tools (NBTs) to achieve higher expression levels of GOIs in, for example, the root or flower bud for the efficient control of phytonematodes and pest-insects, respectively, in important crops.
650 $aGene expression
650 $aAlgodão
650 $aGene Marcador
650 $aGenética
653 $aCotton constitutive promoter
653 $aNew biotechnological tools
653 $aTranscriptional core promoter
653 $aTransgenic crops
700 1 $aLOURENCO-TESSUTTI, I. T.
700 1 $aBUSANELLO, C.
700 1 $aPINTO, C. E. M.
700 1 $aFREITAS, E. de O.
700 1 $aRIBEIRO, T. P.
700 1 $aENGLER, J. de A.
700 1 $aOLIVEIRA, A. C. de
700 1 $aMORGANTE, C. V.
700 1 $aALVES-FERREIRA, M.
700 1 $aGROSSI-DE-SA, M. F.
773 $tPlanta$gv. 251, n. 2, 2020.
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