Data/hora: 01/06/2024 05:37:37

Biblioteca(s): | Embrapa Recursos Genéticos e Biotecnologia; Embrapa Semiárido.
Data corrente: | 18/09/2020
Data da última atualização: | 17/11/2020
Autoria: | MELO, B. P. de; LOURENCO-TESSUTTI, I. T.; MORGANTE, C. V.; SANTOS, N. C.; PINHEIRO, L. B.; LINS, C. B. de J.; SILVA, M. C. M.; MACEDO, L. L. P.; FONTES, E. P. B.; GROSSI-DE-SA, M. F.
Afiliação: | BRUNO PAES DE MELO, UFV; ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen; CAROLINA VIANNA MORGANTE, CPATSA; NAIARA CORDEIRO SANTOS; LUANNA BEZERRA PINHEIRO, UCB; CAMILA BARROZO DE JESUS LINS; MARIA CRISTINA MATTAR DA SILVA, Cenargen; LEONARDO LIMA PEPINO DE MACEDO, Cenargen; ELIZABETH PACHECO BATISTA FONTES, UFV; MARIA FATIMA GROSSI DE SA, Cenargen.
Título: | Soybean embryonic axis transformation: combining biolistic and Agrobacterium-Mediated Protocols to overcome typical complications of in vitro plant regeneration.
Ano de publicação: | 2020
Fonte/Imprenta: | Frontiers in Plant Science, v. 11, article 1228, 2020.
DOI: | https://doi.org/10.3389/fpls.2020.01228
Idioma: | Inglês
Conteúdo: | The first successful attempt to generate genetically modified plants expressing a transgene was preformed via T-DNA-based gene transfer employing Agrobacterium tumefaciens-mediated genetic transformation. Limitations over infectivity and in vitro tissue culture led to the development of other DNA delivery systems, such as the biolistic method. Herein, we developed a new one-step protocol for transgenic soybean recovery by combining the two different transformation methods. This protocol comprises the following steps: agrobacterial preparation, seed sterilization, soybean embryo excision, shoot-cell injury by tungsten-microparticle bombardment, A. tumefaciens-mediated transformation, embryo co-cultivation in vitro, and selection of transgenic plants. This protocol can be completed in approximately 30?40 weeks. The average efficiency of producing transgenic soybean germlines using this protocol was 9.84%, similar to other previously described protocols. However, we introduced a more cost-effective, more straightforward and shorter methodology for transgenic plant recovery, which allows co-cultivation and plant regeneration in a single step, decreasing the chances of contamination and making the manipulation easier. Finally, as a hallmark, our protocol does not generate plant chimeras, in contrast to traditional plant regeneration protocols applied in other Agrobacterium-mediated transformation methods. Therefore, this new approach of plant transformation is applicable for studies of gene function and the production of transgenic cultivars carrying different traits for precision-breeding programs.
Palavras-Chave: | Agrobacterium-mediated transformation; Embryonic axis; High-efficiency plant transformation; Particle bombardment; Planta geneticamente modificada; Recuperação trangênica de soja.
Thesagro: | Cultura de Tecido; Glycine Max; Soja.
Thesaurus Nal: | Agrobacterium; Embryonic structures; Genetic transformation; Plant genetics.

Categoria do assunto:

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G Melhoramento Genético

URL: | http://ainfo.cnptia.embrapa.br/digital/bitstream/item/216085/1/fpls-11-01228.pdf
Registro original: | Embrapa Recursos Genéticos e Biotecnologia (CENARGEN)

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