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Registro Completo |
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Biblioteca(s): |
Embrapa Agricultura Digital. |
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Data corrente: |
09/01/2020 |
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Data da última atualização: |
04/02/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
ZANELLA, L.; DEGENHARDT-GOLDBACH, J.; GERHARDT, I. R.; BESPALHOK FILHO, J. C. |
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Afiliação: |
LAUDIANE ZANELLA, UFPR; JULIANA DEGENHARDT GOLDBACH, CNPF; ISABEL RODRIGUES GERHARDT, CNPTIA; JOÃO CARLOS BESPALHOK FILHO, UFPR. |
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Título: |
RNA interference supression of DOF transcription factor from Populus tremula x Populus alba. |
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Ano de publicação: |
2019 |
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Fonte/Imprenta: |
Pesquisa Florestal Brasileira, v. 39, e201902043, p. 322, 2019. |
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Idioma: |
Inglês |
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Notas: |
Na publicação: Isabel Gerhardt. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba. |
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Conteúdo: |
From the total biomass produced by plants, 70% is represented by cell wall, in which carbon is mainly accumulated in the secondary wall. Therefore, the understanding of the mechanisms that regulate cell wall synthesis and its modification through genetic engineering may lead to the increase of lignocellulosic biomass. Among several functions already attributed to the proteins of the Dof transcription factors, its relation with cell wall formation is one of the most studied. However, the mechanisms involved in this process are not fully elucidated. Populus is the most used model plant for studies with forest species, including functional genomics. The objective of this work was to silence one gene of the Dof family by RNAi in plants of Populus tremula x P. alba, and investigate its function, aiming at the future production of Eucalyptus and Pinus plants with higher biomass production. For this, a silencing vector was constructed with specific primers of a Dof family gene from Populus. To obtain the transgenic plants via Agrobacterium tumefaciens, root segments were used as initial explants. Fifteen events were obtained, and in twelve the Dof expression was suppressed at different levels, according to qPCR analyzes. Of these, on five the expression was highly suppressed, indicating that the gene was successfully silenced. In morphological evaluations after 90 days the events did not present phenotypic differences in relation to non transformed plants. Physiological and histochemical analyzes are underway to evaluate lignin and cellulose contents and structural changes in transformed plants. MenosFrom the total biomass produced by plants, 70% is represented by cell wall, in which carbon is mainly accumulated in the secondary wall. Therefore, the understanding of the mechanisms that regulate cell wall synthesis and its modification through genetic engineering may lead to the increase of lignocellulosic biomass. Among several functions already attributed to the proteins of the Dof transcription factors, its relation with cell wall formation is one of the most studied. However, the mechanisms involved in this process are not fully elucidated. Populus is the most used model plant for studies with forest species, including functional genomics. The objective of this work was to silence one gene of the Dof family by RNAi in plants of Populus tremula x P. alba, and investigate its function, aiming at the future production of Eucalyptus and Pinus plants with higher biomass production. For this, a silencing vector was constructed with specific primers of a Dof family gene from Populus. To obtain the transgenic plants via Agrobacterium tumefaciens, root segments were used as initial explants. Fifteen events were obtained, and in twelve the Dof expression was suppressed at different levels, according to qPCR analyzes. Of these, on five the expression was highly suppressed, indicating that the gene was successfully silenced. In morphological evaluations after 90 days the events did not present phenotypic differences in relation to non transformed plants. Physiological and histoch... Mostrar Tudo |
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Thesaurus Nal: |
Populus alba; Populus tremula. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02291nam a2200181 a 4500
001 2118433
005 2020-02-04
008 2019 bl uuuu u01u1 u #d
100 1 $aZANELLA, L.
245 $aRNA interference supression of DOF transcription factor from Populus tremula x Populus alba.$h[electronic resource]
260 $aPesquisa Florestal Brasileira, v. 39, e201902043, p. 322$c2019
500 $aNa publicação: Isabel Gerhardt. Edição especial dos resumos do IUFRO World Congress, 25., 2019, Curitiba.
520 $aFrom the total biomass produced by plants, 70% is represented by cell wall, in which carbon is mainly accumulated in the secondary wall. Therefore, the understanding of the mechanisms that regulate cell wall synthesis and its modification through genetic engineering may lead to the increase of lignocellulosic biomass. Among several functions already attributed to the proteins of the Dof transcription factors, its relation with cell wall formation is one of the most studied. However, the mechanisms involved in this process are not fully elucidated. Populus is the most used model plant for studies with forest species, including functional genomics. The objective of this work was to silence one gene of the Dof family by RNAi in plants of Populus tremula x P. alba, and investigate its function, aiming at the future production of Eucalyptus and Pinus plants with higher biomass production. For this, a silencing vector was constructed with specific primers of a Dof family gene from Populus. To obtain the transgenic plants via Agrobacterium tumefaciens, root segments were used as initial explants. Fifteen events were obtained, and in twelve the Dof expression was suppressed at different levels, according to qPCR analyzes. Of these, on five the expression was highly suppressed, indicating that the gene was successfully silenced. In morphological evaluations after 90 days the events did not present phenotypic differences in relation to non transformed plants. Physiological and histochemical analyzes are underway to evaluate lignin and cellulose contents and structural changes in transformed plants.
650 $aPopulus alba
650 $aPopulus tremula
700 1 $aDEGENHARDT-GOLDBACH, J.
700 1 $aGERHARDT, I. R.
700 1 $aBESPALHOK FILHO, J. C.
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Registro original: |
Embrapa Agricultura Digital (CNPTIA) |
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Biblioteca |
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Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
06/01/2017 |
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Data da última atualização: |
01/06/2020 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
OLIVEIRA, J. E. de M.; LOPES, F. S. C.; FERNANDES, M. H. de A. |
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Afiliação: |
JOSE EUDES DE MORAIS OLIVEIRA, CPATSA; FABIANA SOARES CARIRI LOPES, Universidade Federal Rural de Pernambuco; MARIA HERLÂNDIA DE A. FERNANDES, UNIVASF. |
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Título: |
Vine pest in Northeast of Brazil: a challenge for the production? |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM ON TROPICAL WINES, 5., 2016, Petrolina. Book of abstracts... Petrolina: Embrapa Semiárido, 2016. |
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Páginas: |
p. 21. |
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Idioma: |
Inglês |
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Conteúdo: |
The vines producing areas in the São Francisco Valley, located in northeastern Brazil, is the second national pole of the grape production of wines and juices and the main producer of table grapes for in natura consumption. With the increase of cultivated areas in the region, it is the expansion of the geographical distribution of insect pests by dispersing and/or involuntary transport of infested materials, serious problems of the phytosanitary order occur frequently. Thus, this factor is considered a major obstacle to the development of culture in this region affecting productivity and product quality. The insect pests can occur throughout the year attacking various parts of culture, especially in bunches. In Brazil, listed about 40 species of arthropods attacking the growing of vines. However, depending on the region, few species cause significant damage to production (Oliveira et al., 2010). Among the pests that attack the vine in the São Francisco Valley, stands the two mites species Polyphagotarsonemus latus (Banks, 1904) (Acari: Tarsonemidae) and Tetranychus urticae (Koch, 1836) (Acari : Tetranychidae); three thrips species Retithrips syriacus (Mayet, 1890), Selenothrips rubrocinctus (Giard, 1901) and Frankliniella sp. (Thysanoptera: Thripidae); any species of lepidopterous Cryptoblabes gnidiella (Millière, 1864) (Lepidoptera: Pyralidae), Spodoptera frugiperda (Smith, 1797), Spodoptera eridania (Stoll, 1782), Spodoptera cosmioides (Walker, 1858), (Lepidoptera: Noctuidae), Helicoverpa armigera (Hübner, 1805) (Lepidoptera: Noctuidae); cochineal Eurhizococcus brasiliensis (Hempel, 1922) (Hemiptera: Margarodidae) and mealybugs Planococcus citri (Risso, 1813), Dysmicoccus brevipes (Cockerell, 1893) e Phenacoccus solenopsis Tinsley, 1898 (Hemiptera: Pseudococcidae). Recently, it was found the presence of pink hibiscus mealybug, Maconelicoccus hirsutus (Green, 1908) (Hemiptera: Pseudococcidae), becoming a factor to be studied due to the damage that can lead to the growing of vines, as reported in other countries (Vitullo, 2009). Based on the above, is necessary knowledge of the dynamics, habits, damage and time of occurrence of each species, they are fundamental importance for effective control measures to used safely and rationally. MenosThe vines producing areas in the São Francisco Valley, located in northeastern Brazil, is the second national pole of the grape production of wines and juices and the main producer of table grapes for in natura consumption. With the increase of cultivated areas in the region, it is the expansion of the geographical distribution of insect pests by dispersing and/or involuntary transport of infested materials, serious problems of the phytosanitary order occur frequently. Thus, this factor is considered a major obstacle to the development of culture in this region affecting productivity and product quality. The insect pests can occur throughout the year attacking various parts of culture, especially in bunches. In Brazil, listed about 40 species of arthropods attacking the growing of vines. However, depending on the region, few species cause significant damage to production (Oliveira et al., 2010). Among the pests that attack the vine in the São Francisco Valley, stands the two mites species Polyphagotarsonemus latus (Banks, 1904) (Acari: Tarsonemidae) and Tetranychus urticae (Koch, 1836) (Acari : Tetranychidae); three thrips species Retithrips syriacus (Mayet, 1890), Selenothrips rubrocinctus (Giard, 1901) and Frankliniella sp. (Thysanoptera: Thripidae); any species of lepidopterous Cryptoblabes gnidiella (Millière, 1864) (Lepidoptera: Pyralidae), Spodoptera frugiperda (Smith, 1797), Spodoptera eridania (Stoll, 1782), Spodoptera cosmioides (Walker, 1858), (Lepidoptera: Noctuid... Mostrar Tudo |
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Palavras-Chave: |
Vale do São Francisco. |
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Thesagro: |
Doença; Inseto; Polyphagotarsonemus Latus; Praga; Tetranychus Urticae; Uva. |
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Thesaurus NAL: |
Grapes. |
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Categoria do assunto: |
O Insetos e Entomologia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/156637/1/Eudes.pdf
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Marc: |
LEADER 03023nam a2200241 a 4500
001 2060140
005 2020-06-01
008 2016 bl uuuu u00u1 u #d
100 1 $aOLIVEIRA, J. E. de M.
245 $aVine pest in Northeast of Brazil$ba challenge for the production?$h[electronic resource]
260 $aIn: INTERNATIONAL SYMPOSIUM ON TROPICAL WINES, 5., 2016, Petrolina. Book of abstracts... Petrolina: Embrapa Semiárido$c2016
300 $ap. 21.
520 $aThe vines producing areas in the São Francisco Valley, located in northeastern Brazil, is the second national pole of the grape production of wines and juices and the main producer of table grapes for in natura consumption. With the increase of cultivated areas in the region, it is the expansion of the geographical distribution of insect pests by dispersing and/or involuntary transport of infested materials, serious problems of the phytosanitary order occur frequently. Thus, this factor is considered a major obstacle to the development of culture in this region affecting productivity and product quality. The insect pests can occur throughout the year attacking various parts of culture, especially in bunches. In Brazil, listed about 40 species of arthropods attacking the growing of vines. However, depending on the region, few species cause significant damage to production (Oliveira et al., 2010). Among the pests that attack the vine in the São Francisco Valley, stands the two mites species Polyphagotarsonemus latus (Banks, 1904) (Acari: Tarsonemidae) and Tetranychus urticae (Koch, 1836) (Acari : Tetranychidae); three thrips species Retithrips syriacus (Mayet, 1890), Selenothrips rubrocinctus (Giard, 1901) and Frankliniella sp. (Thysanoptera: Thripidae); any species of lepidopterous Cryptoblabes gnidiella (Millière, 1864) (Lepidoptera: Pyralidae), Spodoptera frugiperda (Smith, 1797), Spodoptera eridania (Stoll, 1782), Spodoptera cosmioides (Walker, 1858), (Lepidoptera: Noctuidae), Helicoverpa armigera (Hübner, 1805) (Lepidoptera: Noctuidae); cochineal Eurhizococcus brasiliensis (Hempel, 1922) (Hemiptera: Margarodidae) and mealybugs Planococcus citri (Risso, 1813), Dysmicoccus brevipes (Cockerell, 1893) e Phenacoccus solenopsis Tinsley, 1898 (Hemiptera: Pseudococcidae). Recently, it was found the presence of pink hibiscus mealybug, Maconelicoccus hirsutus (Green, 1908) (Hemiptera: Pseudococcidae), becoming a factor to be studied due to the damage that can lead to the growing of vines, as reported in other countries (Vitullo, 2009). Based on the above, is necessary knowledge of the dynamics, habits, damage and time of occurrence of each species, they are fundamental importance for effective control measures to used safely and rationally.
650 $aGrapes
650 $aDoença
650 $aInseto
650 $aPolyphagotarsonemus Latus
650 $aPraga
650 $aTetranychus Urticae
650 $aUva
653 $aVale do São Francisco
700 1 $aLOPES, F. S. C.
700 1 $aFERNANDES, M. H. de A.
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Embrapa Semiárido (CPATSA) |
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