Data/hora: 18/06/2024 07:18:33

Biblioteca(s): | Embrapa Arroz e Feijão; Embrapa Meio Ambiente.
Data corrente: | 13/09/2022
Data da última atualização: | 14/09/2022
Autoria: | LOCATELLI, G. O.; PIMENTEL, M. F.; BUENO, L. A.; LOBO JUNIOR, M.; MASCARIN, G. M.; FINKLER, C. L. L.
Afiliação: | GABRIEL OLIVO LOCATELLI, UNIVERSIDADE FEDERAL RURAL DE PERNAMBUCO; MARIA FERNANDA PIMENTEL, UNIVERSIDADE FEDERAL DE PERNAMBUCO; LUCIANO AVALLONE BUENO, UNIVERSIDADE FEDERAL DO ABC; MURILLO LOBO JUNIOR, CNPAF; GABRIEL MOURA MASCARIN, CNPMA; CHRISTINE LAMENHA LUNA FINKLER, UNIVERSIDADE FEDERAL DE PERNAMBUCO.
Título: | Production of microsclerotia by Trichoderma asperellum through submerged liquid fermentation using low-cost nitrogen and carbon sources.
Ano de publicação: | 2022
Fonte/Imprenta: | Biocatalysis and Agricultural Biotechnology, v. 44, article 102455, 2022.
ISSN: | 1878-8181
DOI: | https://doi.org/10.1016/j.bcab.2022.102455
Idioma: | Inglês
Conteúdo: | Abstract: The aim of this work is to optimize the nutritional conditions using low-cost carbon and nitrogen sources for microsclerotia (MS) production of two Brazilian Trichoderma asperellum strains (BRM-29576 and BRM-29104). A fractional factorial design was used to investigate five variables: carbon source, carbon concentration, nitrogen source, C:N ratio and fungal strain. In this study, the optimal nutritional conditions for improving MS yield were achieved using sucrose and autolyzed yeast, carbon concentration at 20 g L-1 and C:N ratio of 10:1 for the BRM-29104 strain, which outperformed the other strain and yielded up to 2.5 (±0.9) × 104 MS mL-1 within 7 days of fermentation. Sucrose and autolyzed yeast are two economically available carbon and nitrogen sources in Brazil that are suitable for MS production by T. asperellum BRM-29104. Vacuum filtration of MS biomass through nylon filter (200 mesh/74 micrometer) increased MS concentration, resulting in up to 1.8 (±0.2) × 105 MS g-1. Our results support a cost-efficient liquid medium to achieve high MS yields by strain BRM-29104 in combination with an easy method to harvest and concentrate MS from culture broth for further formulation development.
Palavras-Chave: | Autolyzed yeast; Biofungicides; C N ratio; Microsclerotia.
Thesagro: | Carbono; Farinha; Fermentação; Fungo Para Controle Biológico; Nitrogênio; Sorgo; Trichoderma.
Thesaurus Nal: | Biological control agents; Liquid state fermentation; Sorghum flour; Trichoderma asperellum.

Categoria do assunto:

H Saúde e Patologia
X Pesquisa, Tecnologia e Engenharia

Registro original: | Embrapa Arroz e Feijão (CNPAF)

Exemplares:

CNPMA - (17231-1) - UPC - AP - DD -  -  -  - DIS
CNPAF - (36504-1) - UPC - AP - DD - 2022 -  - 2022 - DIS