Data/hora: 08/06/2024 01:33:31

Biblioteca(s): 	Embrapa Mandioca e Fruticultura.	
Data corrente: 	25/03/2011	
Data da última atualização: 	30/03/2012	
Autoria: 	KUBO, K. S.; NOVELLI, V. M.; BASTIANEL, M.; LOCALI-FABRIS, E. C.; ANTONIOLI-LUIZON, R.; MACHADO, M. A.; FREITAS-ASTUA, J.	
Afiliação: 	Karen Sumire Kubo, IAC; Valdenice Moreira Novelli, IAC; Marinês Bastianel, APTA; Eliane Cristina Locali-Fabris, IAC; Renata Antonioli-Luizon, IAC; Marcos Antônio Machado, IAC; JULIANA DE FREITAS ASTUA, CNPMF.	
Título: 	Detection of Brevipalpus-transmitted viruses in their mite vectors by RT-PCR.	
Ano de publicação: 	2011	
Fonte/Imprenta: 	Experimental Applied Acarology, Dordrechet, v. 54, n. 1, jan. 2011.	
ISBN: 	ISSN 1572-9702 (Online)	
DOI: 	10.1007/s10493-011-9425-9	
Idioma: 	Inglês	
Conteúdo: 	The diagnosis of plant diseases caused by Brevipalpus-transmitted viruses (BrTVs) has been done through the analyses of symptoms, transmission electron microscopy, and RT-PCR of infected plant tissues. Here, we report the detection of Citrus leprosis virus C, Orchid fleck virus, Clerodendrum chlorotic spot virus and Solanum violaefolium ringspot virus in their viruliferous vectors Brevipalpus spp. using specific primer pairs for each of the viruses. The efficiency of virus transmission by Brevipalpus mites is low, so the detection of these pathogens in their vectors could constitute an important tool for studies involving virus-vector relationships, transmission, and monitoring the pathogen prior to the appearance of symptoms in the field.	
Palavras-Chave: 	BrTV; Leprosis; RT-PCR; Virus detection.	
Thesaurus Nal: 	Citrus; Tenuipalpidae.	
Categoria do assunto: 	X Pesquisa, Tecnologia e Engenharia	
Registro original: 	Embrapa Mandioca e Fruticultura (CNPMF)	
Exemplares: 	CNPMF - (27593-1) - UPC - SP - PP - S12947 -  - 12947 - PCT