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 | Acesso ao texto completo restrito à biblioteca da Embrapa Pecuária Sudeste. Para informações adicionais entre em contato com cppse.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Pecuária Sudeste. |
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Data corrente: |
21/12/2010 |
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Data da última atualização: |
30/06/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
BERNARDI, A. C. de C.; MONTE, M. B. M.; PAIVA, P. R. P.; MENDONÇA, F. C.; OLIVEIRA, P. P. A.; POLIDORO, J. C.; WERNECK, C. G.; HAIM, P. G.; SOUZA-BARROS, F. |
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Afiliação: |
ALBERTO CARLOS DE CAMPOS BERNARDI, CPPSE; M. B. M. MONTE, CETEM/RIO DE JANEIRO, RJ; P. R. P. PAIVA, CETEM/RIO DE JANEIRO, RJ; FERNANDO C. MENDONÇA, ESALQ/PIRACICABA; PATRICIA PERONDI ANCHAO OLIVEIRA, CPPSE; JOSE CARLOS POLIDORO, CNPS; C. G. WERNECK, UFRRJ/SEROPÉDICA, RJ; P. G. HAIM, UFRRJ/SEROPÉDICA, RJ; F. SOUZA-BARROS, UFRJ/RIO DE JANEIRO, RJ. |
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Título: |
Potential of agricultural use of Brazilian sedimentary zeolite. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
In: INTERNATIONAL CONFERENCE OF THE OCCURENCE, PROPERTIES, AND UTILIZATION OF NATURAL ZEOLITES, 8., 2010, Sofia, Bulgaria. Proceedings... Sofia: INZA, 2010. |
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Páginas: |
p. 47-48 |
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Idioma: |
Português |
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Palavras-Chave: |
Brasilian mineral potetial; Zeolite. |
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Thesaurus Nal: |
agriculture. |
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Categoria do assunto: |
H Saúde e Patologia |
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Marc: |
LEADER 00811nam a2200241 a 4500
001 1870570
005 2023-06-30
008 2010 bl uuuu u00u1 u #d
100 1 $aBERNARDI, A. C. de C.
245 $aPotential of agricultural use of Brazilian sedimentary zeolite.$h[electronic resource]
260 $aIn: INTERNATIONAL CONFERENCE OF THE OCCURENCE, PROPERTIES, AND UTILIZATION OF NATURAL ZEOLITES, 8., 2010, Sofia, Bulgaria. Proceedings... Sofia: INZA$c2010
300 $ap. 47-48
650 $aagriculture
653 $aBrasilian mineral potetial
653 $aZeolite
700 1 $aMONTE, M. B. M.
700 1 $aPAIVA, P. R. P.
700 1 $aMENDONÇA, F. C.
700 1 $aOLIVEIRA, P. P. A.
700 1 $aPOLIDORO, J. C.
700 1 $aWERNECK, C. G.
700 1 $aHAIM, P. G.
700 1 $aSOUZA-BARROS, F.
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Registro original: |
Embrapa Pecuária Sudeste (CPPSE) |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/07/2013 |
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Data da última atualização: |
18/12/2013 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 1 |
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Autoria: |
CALEGARIO, R. F.; LOCALI, E. C.; STACH-MACHADO, D. R.; PERON, L. A.; CASERTA, R.; SALAROLI, R. B.; ASTUA, J. de F.; MACHADO, M. A.; KITAJIMA, E. W. |
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Afiliação: |
RENATA F. CALEGARIO, ESALQ; ELIANE C. LOCALI, APTA; DAGMAR R. STACH-MACHADO; LUÍS ANTÔNIO PERON; RAQUEL CASERTA, APTA; RENATO B. SALAROLI, ESALQ; JULIANA DE FREITAS ASTUA, CNPMF; MARCOS A. MACHADO, APTA; ELLIOT W. KITAJIMA, ESALQ. |
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Título: |
Polyclonal antibodies to the putative coat protein of Citrus leprosis virus C expressed in Escherichia coli: production and use in immunodiagnosis. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
Tropical Plant Pathology, v.38 n.3 Brasília May/June 2013. |
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Idioma: |
Inglês |
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Notas: |
Epub May 14, 2013. |
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Conteúdo: |
This work reports the in vitro expression of Citrus leprosis virus C (CiLV-C) putative coat protein (p29) and the production of a polyclonal antibody to be used as a tool for serological diagnosis of citrus leprosis. The ORF2/RNA1, corresponding to p29, was cloned in pET28a and transformed into Escherichia coli cells (BL21). Expression of p29 was induced in vitro and the protein was purified and used for immunization of rabbits to produce the polyclonal antibody. The anti p29 serum was shown to be highly specific to CiLV-C detection by immunological methods (Western blot, PTA-ELISA, tissue blot and in situ immunolocalization), without cross reaction with healthy citrus plants or other cytoplasmic and nuclear viruses transmitted by Brevipalpus mites. These results demonstrate that the antibody against CiLV-C p29 protein is highly specific for CiLV-C detection. In situ immunogold labeling assays on thin sections of sweet orange leaf cells infected by CiLV-C demonstrated that short, bacilliform particles present within cisternae of the endoplasmic reticulum were specifically labeled, confirming their viral nature. The dense cytoplasmic viroplasm was also heavily labeled indicating that it represents a site of p29 accumulation. |
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Thesagro: |
Fruta citríca. |
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Thesaurus NAL: |
Citrus; Citrus leprosis virus C; Electron microscopy; Immunoassays. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02121naa a2200289 a 4500
001 1961973
005 2013-12-18
008 2013 bl uuuu u00u1 u #d
100 1 $aCALEGARIO, R. F.
245 $aPolyclonal antibodies to the putative coat protein of Citrus leprosis virus C expressed in Escherichia coli$bproduction and use in immunodiagnosis.
260 $c2013
500 $aEpub May 14, 2013.
520 $aThis work reports the in vitro expression of Citrus leprosis virus C (CiLV-C) putative coat protein (p29) and the production of a polyclonal antibody to be used as a tool for serological diagnosis of citrus leprosis. The ORF2/RNA1, corresponding to p29, was cloned in pET28a and transformed into Escherichia coli cells (BL21). Expression of p29 was induced in vitro and the protein was purified and used for immunization of rabbits to produce the polyclonal antibody. The anti p29 serum was shown to be highly specific to CiLV-C detection by immunological methods (Western blot, PTA-ELISA, tissue blot and in situ immunolocalization), without cross reaction with healthy citrus plants or other cytoplasmic and nuclear viruses transmitted by Brevipalpus mites. These results demonstrate that the antibody against CiLV-C p29 protein is highly specific for CiLV-C detection. In situ immunogold labeling assays on thin sections of sweet orange leaf cells infected by CiLV-C demonstrated that short, bacilliform particles present within cisternae of the endoplasmic reticulum were specifically labeled, confirming their viral nature. The dense cytoplasmic viroplasm was also heavily labeled indicating that it represents a site of p29 accumulation.
650 $aCitrus
650 $aCitrus leprosis virus C
650 $aElectron microscopy
650 $aImmunoassays
650 $aFruta citríca
700 1 $aLOCALI, E. C.
700 1 $aSTACH-MACHADO, D. R.
700 1 $aPERON, L. A.
700 1 $aCASERTA, R.
700 1 $aSALAROLI, R. B.
700 1 $aASTUA, J. de F.
700 1 $aMACHADO, M. A.
700 1 $aKITAJIMA, E. W.
773 $tTropical Plant Pathology$gv.38 n.3 Brasília May/June 2013.
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Embrapa Mandioca e Fruticultura (CNPMF) |
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