Data/hora: 04/06/2024 18:22:49

Biblioteca(s): 	Embrapa Caprinos e Ovinos; Embrapa Pecuária Sul; Embrapa Recursos Genéticos e Biotecnologia.	
Data corrente: 	07/12/2012	
Data da última atualização: 	22/01/2016	
Autoria: 	LOBO, A. M. B. O.; GUIMARÃES, S. E. F.; PAIVA, S. R.; CARDOSO, F. F.; SILVA, F. F.; FERNANDES JÚNIOR, G. A.; LOBO, R. N. B.	
Afiliação: 	ANA MARIA BEZERRA OLIVEIRA LOBO, CNPC; SIMONE ELIZA FACCIONI GUIMARÃES, UFV; SAMUEL REZENDE PAIVA, CENARGEN; FERNANDO FLORES CARDOSO, CPPSUL; FABYANO FONSECA SILVA, UFV; GERARDO ALVES FERNANDES JÚNIOR, UNESP; RAIMUNDO NONATO BRAGA LOBO, CNPC.	
Título: 	Differentially transcribed genes in skeletal muscle of lambs	
Ano de publicação: 	2012	
Fonte/Imprenta: 	Livestock Science, Amsterdam, v. 150, n. 1/3, p. 31-41, Dec. 2012.	
Idioma: 	Inglês
Português	
Conteúdo: 	Abstract: The objective of this study was to compare gene transcription profiles in Longissimus dorsi muscle of the following four hair sheep genetic groups, Morada Nova (MO), Brazilian Somali (SO), Santa Inês (SI) and ½Dorper×½Morada Nova (F1). These groups all display different postnatal muscle growth. The transcriptomes of the skeletal muscle of the lambs (at 200 days of age) were profiled by using oligonucleotide microarrays and reverse transcription-quantitative real-time PCR (RT-qPCR). The microarray experiment identified 262 transcripts that were differentially expressed when transcription levels were compared between the different breeds. A total of 23 transcripts among which those involved in skeletal muscle development (MyoD1 and IGFBP4), lipogenesis and adipogenesis (C/EBP?, PPAR? and PGDS) were differentially expressed in at least in one comparison. Clustering analysis showed that there is greater similarity in gene expression between the MO and SI breeds and between F1 and SO genetic groups. The SO breed has the most distinct expression pattern. The RT-qPCR results confirmed the findings from the microarray study. A positive correlation was observed between the expression of MyoD1 and the cold carcass yield. The negative correlations between the weight and yield of cold carcass with the expression of C/EBP? mean that the selection for adipogenesis could lead to a lower carcass weight. The GLUT3 and PYGL gene transcripts were negatively correlated with fat thickness, but ATP5G1 was positively correlated with this trait. Interestingly, many genes negatively correlated with PUFA were positively correlated with cold carcass yield. In conclusion, the present work demonstrated that there are breed-specific expression patterns in Brazilian hair sheep genetic groups. The differences in gene expression among genetic groups were consistent with their phenotypic differences. The positive correlation of the MyoD1 expression with the cold carcass yield suggests that this gene is important for tissue growth in sheep. The positive correlation of the C/EBP? expression with PUFA provides an opportunity to select for lipid deposition in meat animals.	
Palavras-Chave: 	Cluster; Cluster sampling; Differential expression; IGFBP4; MyoD1.	
Thesagro: 	Genética animal; Ovino; Ovis Aries.	
Thesaurus Nal: 	Animal genetics; cluster analysis; Gene expression; Sheep.	
Categoria do assunto: 	--
G Melhoramento Genético	
Registro original: 	Embrapa Caprinos e Ovinos (CNPC)	
Exemplares: 	CENARGEN - (34185-1) - UPC - AP - DD - SP 20330 -  - SP 20330 - DIS
CPPSUL - (12773-1) - UPC - AP - DD -  -  -  - DIS
CNPC - (29845-1) - UPC - AP - DD -  -  -  - DIS