Data/hora: 10/06/2024 07:58:40

Biblioteca(s): | Embrapa Gado de Corte.
Tipo da produção científica: | Artigo em Periódico Indexado
Circulação/Nível: | B - 3
Data corrente: | 23/10/2009
Data da última atualização: | 01/03/2010
Autoria: | ANDREOTTI, R.; MATOS, M. de F. C.; GONÇALVES, K. N.; OSHIRO, L. M.; LIMA-JUNIOR, M. S. da C.; PAIVA, F.; LEITE, F. L.
Afiliação: | RENATO ANDREOTTI E SILVA, CNPGC; MARIA DE FÁTIMA CEPA MATOS, UFMS; KELLY NODA GONÇALVES, UFMS; LEANDRA MARLA OSHIRO, UFMS; MANOEL SEBASTIÃO DA COSTA LIMA-JUNIOR, UFMS; FERNANDO PAIVA, UFMS; FÁBIO LEIVAS LEITE, UFPel.
Título: | Comparison of indirect ELISA based on recombinant protein NcSRS2 and IFAT for detection of Neospora caninum antibodies in sheep.
Ano de publicação: | 2009
Fonte/Imprenta: | Revista Brasileira de Parasitologia Veterinária, São Paulo, v.18, n.2, p.20-24, abr-jun. 2009.
Idioma: | Inglês

Conteúdo:

Neospora caninum, an Apicomplexan parasite that can causes abortion, is responsible for considerable economic and  reproductive losses in livestock. The purpose of the present study was to determine whether recombinant NcSRS2 is a suitable indirect ELISA antigen for determining specific immune response to N. caninum in sheep. A total of 441 serum samples were subjected to IFAT and rNcSRS2 based-ELISA, with both tests performing similarly. The sensitivity and specificity of indirect ELISA were 98.6 and 98.3%, respectively. The kappa index shows 0.98 concordance between the two tests, which is considered excellent. Seroprevalences of 30.8 and 32.0% were detected by IFAT and indirect ELISA, respectively, showing these tests did not differ significantly on this measure (p > 0.05). Serological analysis showed that HisG
tag was detected by Western Blotting recognizing rNcSRS2 protein. The potential value of rNcSRS2-based ELISA as a highly specific and sensitive tool for serological diagnosis is also supported by the strong agreement found between IFAT and ELISA. The results support the potential use of recombinant protein NcSRS2 as an antigen in indirect ELISA in sheep.

Palavras-Chave: | NcSRS2; Proteína recombinante; Teste.
Thesagro: | Diagnostico; Elisa; Neospora Caninum; Neosporose; Ovino; Sanidade Animal.
Categoria do assunto: | H Saúde e Patologia
Registro original: | Embrapa Gado de Corte (CNPGC)
Exemplares: | CNPGC - (12891-1) - UPC - SP - PP -  -  - 463/18 - DIS