| |
|
|
| Registros recuperados : 11 | |
| 3. |  | ANDREOTTI, R.; GUERRERO, F. D.; SOARES, M. A.; BARROS, J. C.; MILLER, R. J.; PÉREZ DE LEÓN, A. Acaricide resistance of Rhipicephalus (Boophilus) microplus in State of Mato Grosso do Sul, Brazil. Revista Brasileira de Parasitologia Veterinária, Jaboticabal, v. 20, n. 2, p. 127-133, Apr.-Jun. 2011.| Biblioteca(s): Embrapa Gado de Corte. |
|    |
| 5. | | CUNHA, R. C.; PÉREZ DE LEÓN, A. A.; LEITE, F. P. L.; GUERRERO, F. D.; PINTO, L. S.; ANDREOTTI, R. Assessment of bovine immunoprotection against Rhipicephalus microplus using Bm86-CG antigen expressed in Pichia pastoris. In: AMERICAN ASSOCIATION OF VETERINARY PARASITOLOGISTS ANNUAL MEETING, 56.; LIVESTOCK INSECT WORKERS CONFERENCE, 55.; INTERNATIONAL SYMPOSIUM ON ECTOPARASITES OF PETS, 11.,2011,ST. LOUIS, MO. Sustainable parasite and vector control: proceedings. St. Louis,MO: American Association of Veterinary Parasitologists, 2011. p.95| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 6. | | ANDREOTTI, R.; PEREZ DE LEON, A; GUERRERO, F. D.; BENDELE, K. E.; SCOLES, G. A.; DOWD, S. E. Symbiotic bacteria in the cattle tick rhipicephalus microplus. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., 2010, Campo Grande, MS. [Anais...]. Campo Grande, MS: Colégio Brasileiro de Parasitologia Veterinária, 2010. 1 CD-ROM. 1 p.| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 8. | | OLIVEIRA FILHO, J. G. de; FERREIRA, L. L.; SARRIA, A. L. F.; PICKETT, J. A.; MASCARIN, G. M.; PÉREZ DE LEÓN, A. A.; BORGES, L. M. F. Brown dog tick, Rhipicephalus sanguineus sensu lato, infestation of susceptible dog hosts is reduced by slow release of semiochemicals from a less susceptible host. Ticks and Tick-borne Diseases, v. 8, n. 1, p. 139-145, Jan. 2017.| Biblioteca(s): Embrapa Arroz e Feijão. |
|  |
| 9. | | PÉREZ DE LEÓN, A. A.; RODRÍGUEZ VIVAS, I. R.; ROMERO SALAS, D.; ANDREOTTI, R.; ROSARIO CRUZ, R.; CHAPARRO, J.; KLAFKE, G. M.; VILLAR, D.; COSTA-JUNIOR, L.; SOLTERO, F.; TEMEYER, K.; URDAZ, J.; LI, A. Acaricide resistance and strategies to mitigate economic impact of the southern cattle fever tick (Rhipicephalus microplus) on livestock production systems in the Americas. In: INTERNATIONAL CONGRESS OF PARASITOLOGY, 13., 2014, Mexico. Conference abstracts... Mexico City, Mexico: Academic Committee ICOPA Congress, 2014.| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 10. | | BRITO, L. G.; OLIVEIRA, M. C. de S.; FOIL, L. D.; GUERRERO, F. D.; PEREZ DE LEON, A.; BARBIERI, F. da S.; CHAGAS, A. C. de S.; BARROS, A. T. M. de. Characterization of pesticide in horn fly populations as a risk factor for fixation of resistance alleles in cattle tick populations. In: SIMPÓSIO EMBRAPA LABEX EUA DE SANIDADE ANIMAL, 2., Brasília, 2012. Proceedings... Brasília: Embrapa Estudos e Capacitação, 2012. p. 129| Biblioteca(s): Embrapa Pantanal. |
| |
| 11. | | BRITO, L. G.; OLIVEIRA, M. C. de S.; FOIL, l. D.; PEREZ DE LEON, A.; BARBIERI, F. da S.; CHAGAS, A. C. de S.; BARROS, A. T. M. de. Characterization of pesticide resistance in horn fly populations as a risk fator for fixation of resistance alleles in cattle tick populations. In: SIMPÓSIO EMBRAPA LABEX EUA DE SANIDADE ANIMAL, 2., Brasília, 2012. Proceedings... Brasília: Embrapa Estudos e Capacitação, 2012. p. 129.| Biblioteca(s): Embrapa Rondônia. |
| |
| Registros recuperados : 11 | |
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Meio-Norte. |
|
Data corrente: |
10/03/2008 |
|
Data da última atualização: |
14/09/2023 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
Internacional - A |
|
Autoria: |
DINIZ, F. M.; IYENGAR, A.; LIMA, P. S. da C.; MACLEAN, N.; BENTZEN, P. |
|
Afiliação: |
FABIO MENDONCA DINIZ, CPAMN; ARATI IYENGAR; PAULO SARMANHO DA COSTA LIMA, CPAMN; NORMAN MACLEAN; PAUL BENTZEN. |
|
Título: |
Application of a double-enrichment procedure for microsatellite isolation and the use tailed primers for high throughput genotyping. |
|
Ano de publicação: |
2007 |
|
Fonte/Imprenta: |
Genetics and Molecular Biology, São Paulo, v. 30, n. 2, p. 380-384, mar. 2007. |
|
ISSN: |
1415-4757 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The number of microsatellite loci and their allelic diversity contribute to increase accuracy and informativity of genetic estimates, however, the isolation of microsatellite loci is not only laborious but also quite expensive. We used (GATA)n and (GACA)n tetranucleotide probes and single- and double-enrichment hybridization to construct and screen a genomic library with an increased proportion of DNA fragments containing repeat motifs. Repeats were found using both types of hybridization but the double-enrichment procedure recovered sequences of which 100% contained (GATA)n and (GACA)n motifs. Microsatellite loci primers were then designed with an M13R-tail or CAG-tag to produce scorable PCR products with minimal stutter. The approach used in this study suggests that double-enrichment is a worthwhile strategy when isolating repeat motifs from eukaryotic genomes. Moreover, the use of tailed microsatellite primers provides increased resolution for compound microsatellite loci, with a significant decrease in costs. |
|
Palavras-Chave: |
Banda polimórfica; double-enrichment; double-hybridization; Enriquecimento duplo; Hibridação dupla; Microssatélite; stutter bands; tailed microsatellite primer. |
|
Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/65872/1/Application-21449.pdf
|
|
Marc: |
LEADER 01914naa a2200277 a 4500
001 1065872
005 2023-09-14
008 2007 bl uuuu u00u1 u #d
022 $a1415-4757
100 1 $aDINIZ, F. M.
245 $aApplication of a double-enrichment procedure for microsatellite isolation and the use tailed primers for high throughput genotyping.$h[electronic resource]
260 $c2007
520 $aThe number of microsatellite loci and their allelic diversity contribute to increase accuracy and informativity of genetic estimates, however, the isolation of microsatellite loci is not only laborious but also quite expensive. We used (GATA)n and (GACA)n tetranucleotide probes and single- and double-enrichment hybridization to construct and screen a genomic library with an increased proportion of DNA fragments containing repeat motifs. Repeats were found using both types of hybridization but the double-enrichment procedure recovered sequences of which 100% contained (GATA)n and (GACA)n motifs. Microsatellite loci primers were then designed with an M13R-tail or CAG-tag to produce scorable PCR products with minimal stutter. The approach used in this study suggests that double-enrichment is a worthwhile strategy when isolating repeat motifs from eukaryotic genomes. Moreover, the use of tailed microsatellite primers provides increased resolution for compound microsatellite loci, with a significant decrease in costs.
653 $aBanda polimórfica
653 $adouble-enrichment
653 $adouble-hybridization
653 $aEnriquecimento duplo
653 $aHibridação dupla
653 $aMicrossatélite
653 $astutter bands
653 $atailed microsatellite primer
700 1 $aIYENGAR, A.
700 1 $aLIMA, P. S. da C.
700 1 $aMACLEAN, N.
700 1 $aBENTZEN, P.
773 $tGenetics and Molecular Biology, São Paulo$gv. 30, n. 2, p. 380-384, mar. 2007.
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Meio-Norte (CPAMN) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
