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Registro Completo |
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Biblioteca(s): |
Embrapa Territorial. |
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Data corrente: |
15/05/2015 |
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Data da última atualização: |
15/05/2015 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
TEIXEIRA, A. H. de C.; SCHERER-WARREN, M.; HERNANDEZ, F. B. T.; ANDRADE, R. G. |
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Afiliação: |
ANTONIO HERIBERTO DE C TEIXEIRA, CNPM; MORRIS SCHERER-WARREN, ANA; FERNANDO BRAZ TANGERINO HERNANDEZ, UNESP; RICARDO GUIMARAES ANDRADE, CNPM. |
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Título: |
Balanço de energia em larga escala no município de Juazeiro - BA, Brasil. |
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Ano de publicação: |
2013 |
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Fonte/Imprenta: |
In: CONGRESSO BRASILEIRO DE AGROMETEOROLOGIA, 18., 2013, Belém, PA. Belém, PA: Sociedade Brasileira de Agrometeorologia, 2013. |
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Páginas: |
6 p. |
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Idioma: |
Português |
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Conteúdo: |
No município de Juazeiro, Brasil, a vegetação natural vem sendo substituída pela agricultura irrigada, destacando importância para a quantificação dos efeitos da mudança de uso da terra no balanço de energia em larga escala. Imagens MODIS foram usadas para modelagem dos componentes deste balanço na escala municipal, através do algoritmo SAFER. O saldo diário de radiação (Rn) foi obtido através da equação de Slob, possibilitando a quantificação dos fluxos de calor sensível (H) e latente (λE) nesta escala de tempo. As frações médias de Rn fracionadas como H e λE variaram de acordo com as condições termo-hidrológicas ao longo do ano, sendo respectivamente 82 e 18%. Entretanto, maiores valores de λE e menores para H foram verificados nas áreas irrigadas, com alguns casos de advecção de calor detectados, provenientes das vizinhanças das culturas agrícolas. Os modelos aplicados na atual pesquisa podem subsidiar o monitoramento das mudanças climáticas e de uso da terra na escala municipal, sendo valiosas ferramentas para a sustentabilidade dos recursos hídricos no futuro. |
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Palavras-Chave: |
Fluxo de calor latente; Fluxo de calor sensível. |
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Thesagro: |
Sensoriamento Remoto. |
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Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/123944/1/4492.pdf
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Marc: |
LEADER 01806nam a2200193 a 4500
001 2015496
005 2015-05-15
008 2013 bl uuuu u00u1 u #d
100 1 $aTEIXEIRA, A. H. de C.
245 $aBalanço de energia em larga escala no município de Juazeiro - BA, Brasil.$h[electronic resource]
260 $aIn: CONGRESSO BRASILEIRO DE AGROMETEOROLOGIA, 18., 2013, Belém, PA. Belém, PA: Sociedade Brasileira de Agrometeorologia$c2013
300 $a6 p.
520 $aNo município de Juazeiro, Brasil, a vegetação natural vem sendo substituída pela agricultura irrigada, destacando importância para a quantificação dos efeitos da mudança de uso da terra no balanço de energia em larga escala. Imagens MODIS foram usadas para modelagem dos componentes deste balanço na escala municipal, através do algoritmo SAFER. O saldo diário de radiação (Rn) foi obtido através da equação de Slob, possibilitando a quantificação dos fluxos de calor sensível (H) e latente (λE) nesta escala de tempo. As frações médias de Rn fracionadas como H e λE variaram de acordo com as condições termo-hidrológicas ao longo do ano, sendo respectivamente 82 e 18%. Entretanto, maiores valores de λE e menores para H foram verificados nas áreas irrigadas, com alguns casos de advecção de calor detectados, provenientes das vizinhanças das culturas agrícolas. Os modelos aplicados na atual pesquisa podem subsidiar o monitoramento das mudanças climáticas e de uso da terra na escala municipal, sendo valiosas ferramentas para a sustentabilidade dos recursos hídricos no futuro.
650 $aSensoriamento Remoto
653 $aFluxo de calor latente
653 $aFluxo de calor sensível
700 1 $aSCHERER-WARREN, M.
700 1 $aHERNANDEZ, F. B. T.
700 1 $aANDRADE, R. G.
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Registro original: |
Embrapa Territorial (CNPM) |
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Registro Completo
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
03/03/2017 |
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Data da última atualização: |
08/03/2017 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SANTOS, R. C. dos; SILVA, C. R. C. da; SOARES, T. da C; CAVALCANTI, J. J. V.; LIMA, L. M. de; MARTINS, E. S.; PONTES, R. G. M. S. de. |
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Afiliação: |
ROSEANE CAVALCANTI DOS SANTOS, CNPA; CARLIANE REBECA COELHO da SILVA, UFRPE; TAÍZA da CUNHA SOARES, UFRPE; JOSE JAIME VASCONCELOS CAVALCANTI, CNPA; LIZIANE MARIA DE LIMA, CNPA; ÉRICA SOARES MARTINS, Cenargen; ROSE GOMES MONNERAT SOLON DE PONTES, Cenargen. |
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Título: |
Relative expression of CRy10 in GM cotton lines resistant to boll weevil. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: ENCONTRO DE GENÉTICA DO NORDESTE, 21., 2016, Recife. Anais... Ribeirão Preto: SBG; Recife: UFPE: UFRPE: UPE, 2016. p. 87. |
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Idioma: |
Inglês |
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Conteúdo: |
Boll weevil (Anthonomus grandis) is a serious pest of cotton crop in several parts of world. The control is done by synthetic pesticides that increase the management costs. Embrapa coordinates an important research involving development of GM cotton resistant to boll weevil, by genetic transformation. Seven line (T0-8H) containing a Btcry 10 have been identified by feeding bioassays and ELISA, which showed reasonable toxicity against adult boll weevils. In this work, these lines were used in order to estimate the presence and relative expression of cry 10 by PCR and RT-qPCR, respectively, from young buds of 50d-plants. RT-qPCR assays were performed, by using Syber Green Rox Plus Master Mix 2X (LGC). Three endogenous cotton gene (GhACT, GhUBQ14 and GhPP2A) were used as a standard control. At first, 95°C/15 min and 40 cycles of 95°C/ 20 sec, 60°C/ 20 sec and 72°C/ 20 sec. Then, a curve of denaturation (melting curve) was performed after conclusion of amplification, at 95°C/15 sec, 60°C/15 sec, rising 2°/min until reaching 95°C. All reactions were carried out with experimental and biological replications. The threshold cycle (Ct) and PCR efficiency was estimated by Real-time PCR Miner program. The analyses were performed by using qBASEPlus program. Graphics, Cqs and Melt curve were automatically generated, based on the normalization method with a reference gene, ??Cq. Varied levels of expression were found in GM lines, too low in 8H-269 and 8H-357, mid in 8H-282 and 8H-346 and high in 8H- 336 (14x). These data agreed with previous results obtained by ELISA assays. Eleven lines derivate from 8H-336 (T1) were analyzed by PCR assays with genomic DNA, using 2 primer combinations. More than 50% showed amplicons confirming the presence of gene in selected lines. Taking in account that a reasonable level of resistance should overcoming 2 ug of protein/g tissue, we suggested that 8H-366 is the best genotype for control the cotton boll weevil. This material will be further advanced for entomological assays with larvae and adults of boll weevil. MenosBoll weevil (Anthonomus grandis) is a serious pest of cotton crop in several parts of world. The control is done by synthetic pesticides that increase the management costs. Embrapa coordinates an important research involving development of GM cotton resistant to boll weevil, by genetic transformation. Seven line (T0-8H) containing a Btcry 10 have been identified by feeding bioassays and ELISA, which showed reasonable toxicity against adult boll weevils. In this work, these lines were used in order to estimate the presence and relative expression of cry 10 by PCR and RT-qPCR, respectively, from young buds of 50d-plants. RT-qPCR assays were performed, by using Syber Green Rox Plus Master Mix 2X (LGC). Three endogenous cotton gene (GhACT, GhUBQ14 and GhPP2A) were used as a standard control. At first, 95°C/15 min and 40 cycles of 95°C/ 20 sec, 60°C/ 20 sec and 72°C/ 20 sec. Then, a curve of denaturation (melting curve) was performed after conclusion of amplification, at 95°C/15 sec, 60°C/15 sec, rising 2°/min until reaching 95°C. All reactions were carried out with experimental and biological replications. The threshold cycle (Ct) and PCR efficiency was estimated by Real-time PCR Miner program. The analyses were performed by using qBASEPlus program. Graphics, Cqs and Melt curve were automatically generated, based on the normalization method with a reference gene, ??Cq. Varied levels of expression were found in GM lines, too low in 8H-269 and 8H-357, mid in 8H-282 and 8H-346 and ... Mostrar Tudo |
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Palavras-Chave: |
Boll weevil. |
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Thesagro: |
Algodão; Anthonomus Grandis; Bicudo. |
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Thesaurus NAL: |
Cotton. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/157044/1/Relative-expression-of-Cry10.pdf
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Marc: |
LEADER 02851nam a2200241 a 4500
001 2066096
005 2017-03-08
008 2016 bl uuuu u00u1 u #d
100 1 $aSANTOS, R. C. dos
245 $aRelative expression of CRy10 in GM cotton lines resistant to boll weevil.$h[electronic resource]
260 $aIn: ENCONTRO DE GENÉTICA DO NORDESTE, 21., 2016, Recife. Anais... Ribeirão Preto: SBG; Recife: UFPE: UFRPE: UPE, 2016. p. 87.$c2016
520 $aBoll weevil (Anthonomus grandis) is a serious pest of cotton crop in several parts of world. The control is done by synthetic pesticides that increase the management costs. Embrapa coordinates an important research involving development of GM cotton resistant to boll weevil, by genetic transformation. Seven line (T0-8H) containing a Btcry 10 have been identified by feeding bioassays and ELISA, which showed reasonable toxicity against adult boll weevils. In this work, these lines were used in order to estimate the presence and relative expression of cry 10 by PCR and RT-qPCR, respectively, from young buds of 50d-plants. RT-qPCR assays were performed, by using Syber Green Rox Plus Master Mix 2X (LGC). Three endogenous cotton gene (GhACT, GhUBQ14 and GhPP2A) were used as a standard control. At first, 95°C/15 min and 40 cycles of 95°C/ 20 sec, 60°C/ 20 sec and 72°C/ 20 sec. Then, a curve of denaturation (melting curve) was performed after conclusion of amplification, at 95°C/15 sec, 60°C/15 sec, rising 2°/min until reaching 95°C. All reactions were carried out with experimental and biological replications. The threshold cycle (Ct) and PCR efficiency was estimated by Real-time PCR Miner program. The analyses were performed by using qBASEPlus program. Graphics, Cqs and Melt curve were automatically generated, based on the normalization method with a reference gene, ??Cq. Varied levels of expression were found in GM lines, too low in 8H-269 and 8H-357, mid in 8H-282 and 8H-346 and high in 8H- 336 (14x). These data agreed with previous results obtained by ELISA assays. Eleven lines derivate from 8H-336 (T1) were analyzed by PCR assays with genomic DNA, using 2 primer combinations. More than 50% showed amplicons confirming the presence of gene in selected lines. Taking in account that a reasonable level of resistance should overcoming 2 ug of protein/g tissue, we suggested that 8H-366 is the best genotype for control the cotton boll weevil. This material will be further advanced for entomological assays with larvae and adults of boll weevil.
650 $aCotton
650 $aAlgodão
650 $aAnthonomus Grandis
650 $aBicudo
653 $aBoll weevil
700 1 $aSILVA, C. R. C. da
700 1 $aSOARES, T. da C
700 1 $aCAVALCANTI, J. J. V.
700 1 $aLIMA, L. M. de
700 1 $aMARTINS, E. S.
700 1 $aPONTES, R. G. M. S. de
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Registro original: |
Embrapa Algodão (CNPA) |
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