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| 23. |  | BASSOI, L. H.; ASSIS, J. S. de. Distribuicao do sistema radicular de videiras irrigadas em latossolo Vermelho Amarelo do Tropico Semi-Arido. In: CONGRESSO LATINO AMERICANO DE CIENCIA DO SOLO, 13.; REUNIAO BRASILEIRA DE BIOLOGIA DO SOLO, 1.; SIMPOSIO BRASILEIRO SOBRE MICROBIOLOGIA DO SOLO, 4., REUNIAO BRASILEIRA SOBRE MICORRIZA, 6., REUNIAO BRASILEIRA DE MANEJO E CONSERVACAO DE SOLO E DA AGUA, 11., 1996. Aguas de Lindoia, SP. O solo nos ciclos da natureza: anais. Piracicaba. ESALQ/USP/CLCS/SBCS/SBM, 1996. 1 CD-ROM.| Biblioteca(s): Embrapa Semiárido. |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
29/03/2018 |
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Data da última atualização: |
29/03/2018 |
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Autoria: |
SANTOS, E. A. A.; LIMA, G. L.; PRAXEDES, E. C. G.; SILVA, A. M.; MAIA, K. M.; OLIVEIRA, M. F.; RODRIGUES, A. P. R.; SILVA, A. R. |
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Afiliação: |
Erica A. A. Santos, Laboratório de Conservação de Germoplasma Animal/Universidade Federal Rural do Semi-Árido - Ufersa; Gabriela L. Lima, Departamento de Medicina Veterinária/Universidade Federal de Roraima; Erica C. G. Praxedes, Laboratório de Conservação de Germoplasma Animal/Universidade Federal Rural do Semi-Árido - Ufersa; Andréia M. Silva, Laboratório de Conservação de Germoplasma Animal/Universidade Federal Rural do Semi-Árido - Ufersa; Keilla M. Maia, Laboratório de Conservação de Germoplasma Animal/Universidade Federal Rural do Semi-Árido - Ufersa; Moacir F. Oliveira, Laboratório de Conservação de Germoplasma Animal/Universidade Federal Rural do Semi-Árido - Ufersa; Ana Paula R. Rodrigues, Laboratório de Manipulação de Oócitos e Folículos Pré-antrais/Universidade Estadual do Ceará; Alexandre R. Silva, Laboratório de Conservação de Germoplasma Animal/Universidade Federal Rural do Semi-Árido - Ufersa. |
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Título: |
Estimation, morphometry and ultrastructure of ovarian preantral follicle population in agouti (Dasyprocta leporina). |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 38, n. 1, p. 175-182, janeiro 2018. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Morfometria, estimativa e ultraestrutura da população folicular ovariana preantral de cutia (Dasyprocta leporina). |
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Conteúdo: |
The aim of this study was to characterize the preantral ovarian follicular population in agoutis (D. leporina) by estimating the number of follicles at each developmental category, and also describe the morphometry and the specific features of the follicle and the oocyte by using light and transmission electron microscopy. The length of each ovary was measured using a caliper rule, longitudinally sectioned into two halves and both were immediately fixed to perform the estimation of follicular population and ultrastructural analysis. The mean (±S.E.M.) population of follicular per pair of ovary was estimated at 4419.8±532.26 and 5397.52±574.91 for right and left ovaries, respectively, but no differences were observed between them. The diameters for follicles, oocyte and nuclei were: 18.62±3.40μm, 12.28±2.37μm and 6.10±0.93μm for primordial, 23.75±5.70μm, 4.22±3.00μm and 6.70±1.24μm for primary and 88.55±17.61μm, 52.85±17.56μm and 22.33±17.61μm for secondary follicles, respectively. The most of the follicles found belonged to the primordial category (86.63%), followed by primary (13.01%) and secondary (0.35%) one. Additionally, polyovular follicles were observed in all the animals and they represented 7.51% of the total follicles counted. The ultrastructural analysis showed that the oocyte presented a central and regular nuclei, displaying a homogenous mass. Among the organelles, the mitochondria were the most abundant and the oocyte Golgi apparatus was rarely observed. In conclusion, this work shows for the first time the characterization of the population of preantral follicles in the ovary of Dasyprocta leporina. Those information will be useful for further development and adaptation of biotechniques such as germplasm cryopreservation and in vitro gametes manipulation. MenosThe aim of this study was to characterize the preantral ovarian follicular population in agoutis (D. leporina) by estimating the number of follicles at each developmental category, and also describe the morphometry and the specific features of the follicle and the oocyte by using light and transmission electron microscopy. The length of each ovary was measured using a caliper rule, longitudinally sectioned into two halves and both were immediately fixed to perform the estimation of follicular population and ultrastructural analysis. The mean (±S.E.M.) population of follicular per pair of ovary was estimated at 4419.8±532.26 and 5397.52±574.91 for right and left ovaries, respectively, but no differences were observed between them. The diameters for follicles, oocyte and nuclei were: 18.62±3.40μm, 12.28±2.37μm and 6.10±0.93μm for primordial, 23.75±5.70μm, 4.22±3.00μm and 6.70±1.24μm for primary and 88.55±17.61μm, 52.85±17.56μm and 22.33±17.61μm for secondary follicles, respectively. The most of the follicles found belonged to the primordial category (86.63%), followed by primary (13.01%) and secondary (0.35%) one. Additionally, polyovular follicles were observed in all the animals and they represented 7.51% of the total follicles counted. The ultrastructural analysis showed that the oocyte presented a central and regular nuclei, displaying a homogenous mass. Among the organelles, the mitochondria were the most abundant and the oocyt... Mostrar Tudo |
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Palavras-Chave: |
Dasyprocta leporine; Folículos; Morfometria; Ovarian tissue; População folicular ovariana preantral; Tecido ovariano; Ultraestrutura. |
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Thesagro: |
Cutia. |
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Thesaurus NAL: |
Agouti; Morphometry; Ovarian follicles; Ultrastructure. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/174763/1/Estimation-morphometry-and-ultrastructure-of-ovarian-peantral.pdf
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Marc: |
LEADER 03064naa a2200361 a 4500
001 2089954
005 2018-03-29
008 2018 bl uuuu u00u1 u #d
100 1 $aSANTOS, E. A. A.
245 $aEstimation, morphometry and ultrastructure of ovarian preantral follicle population in agouti (Dasyprocta leporina).$h[electronic resource]
260 $c2018
500 $aTítulo em português: Morfometria, estimativa e ultraestrutura da população folicular ovariana preantral de cutia (Dasyprocta leporina).
520 $aThe aim of this study was to characterize the preantral ovarian follicular population in agoutis (D. leporina) by estimating the number of follicles at each developmental category, and also describe the morphometry and the specific features of the follicle and the oocyte by using light and transmission electron microscopy. The length of each ovary was measured using a caliper rule, longitudinally sectioned into two halves and both were immediately fixed to perform the estimation of follicular population and ultrastructural analysis. The mean (±S.E.M.) population of follicular per pair of ovary was estimated at 4419.8±532.26 and 5397.52±574.91 for right and left ovaries, respectively, but no differences were observed between them. The diameters for follicles, oocyte and nuclei were: 18.62±3.40μm, 12.28±2.37μm and 6.10±0.93μm for primordial, 23.75±5.70μm, 4.22±3.00μm and 6.70±1.24μm for primary and 88.55±17.61μm, 52.85±17.56μm and 22.33±17.61μm for secondary follicles, respectively. The most of the follicles found belonged to the primordial category (86.63%), followed by primary (13.01%) and secondary (0.35%) one. Additionally, polyovular follicles were observed in all the animals and they represented 7.51% of the total follicles counted. The ultrastructural analysis showed that the oocyte presented a central and regular nuclei, displaying a homogenous mass. Among the organelles, the mitochondria were the most abundant and the oocyte Golgi apparatus was rarely observed. In conclusion, this work shows for the first time the characterization of the population of preantral follicles in the ovary of Dasyprocta leporina. Those information will be useful for further development and adaptation of biotechniques such as germplasm cryopreservation and in vitro gametes manipulation.
650 $aAgouti
650 $aMorphometry
650 $aOvarian follicles
650 $aUltrastructure
650 $aCutia
653 $aDasyprocta leporine
653 $aFolículos
653 $aMorfometria
653 $aOvarian tissue
653 $aPopulação folicular ovariana preantral
653 $aTecido ovariano
653 $aUltraestrutura
700 1 $aLIMA, G. L.
700 1 $aPRAXEDES, E. C. G.
700 1 $aSILVA, A. M.
700 1 $aMAIA, K. M.
700 1 $aOLIVEIRA, M. F.
700 1 $aRODRIGUES, A. P. R.
700 1 $aSILVA, A. R.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 38, n. 1, p. 175-182, janeiro 2018.
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