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Registro Completo |
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Biblioteca(s): |
Embrapa Instrumentação; Embrapa Mandioca e Fruticultura. |
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Data corrente: |
12/01/2011 |
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Data da última atualização: |
18/06/2013 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
RUSSO, M. S. T.; CARDINALI, M.; MILORI, D. M. B. P.; PEREIRA, F. M. V.; MARTINS, P. K.; FREITAS-ASTÚA, J. |
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Afiliação: |
DEBORA MARCONDES BASTOS P MILORI, CNPDIA. |
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Título: |
Study of laser-induced fluorescence imaging in asymptomatic leaves from citrus plants inoculated with candidatus liberibacter. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Citrus research & technology, Cordeirópolis, v. 31, suplemento, p. 122, 2010. Proceedings of the 18 conference international organization citrus virologists, 2010, Campinas. |
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Idioma: |
Inglês |
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Conteúdo: |
Citrus greening or huanglongbing (HLB) is a devastating disease that has been causing serious problems to citrus cultivation. Some analytical methods have been reported for diagnosis of this disease, mainly DNA-based techniques of Candidatus Liberibacter species, which are the causal agents. In general, most of these developed methods involve PCR (polymerase chain reaction). However, methods based on PCR cannot be used in large-scale to build disease distribution maps. The visual inspection, usually adopted, can lead to misleading diagnoses, since the symptoms are easily mistaken with others problems and the disease has a long asymptomatic phase. Aiming the development of a new method for early diagnosis of this malady, leaves from inoculated and healthy (control group) plants were assessed using laser- induced fluorescence imaging (LIFI). The plants were measured monthly and the evolution of the bacteria on inoculated plants was monitored by RT-qPCR (real-time quantitative PCR) amplification of CLas sequences. A laser emitting at 470 nm was used to excite the fluorescence of chlorophyll and other metabolites present on the leaf. Each image was associated to histograms of colours (colourgrams) and a matrix. For the investigations of data, principal components analysis were made for the categories of the colourgram (scales of red, blue and green, luminosity, relative red, blue and green, hue, saturation and intensity). Preliminary results showed that there are differences in the images obtained from healthy and inoculated plants, indicating a great potential of the technique to perform early diagnoses in large scale that are economically viable. MenosCitrus greening or huanglongbing (HLB) is a devastating disease that has been causing serious problems to citrus cultivation. Some analytical methods have been reported for diagnosis of this disease, mainly DNA-based techniques of Candidatus Liberibacter species, which are the causal agents. In general, most of these developed methods involve PCR (polymerase chain reaction). However, methods based on PCR cannot be used in large-scale to build disease distribution maps. The visual inspection, usually adopted, can lead to misleading diagnoses, since the symptoms are easily mistaken with others problems and the disease has a long asymptomatic phase. Aiming the development of a new method for early diagnosis of this malady, leaves from inoculated and healthy (control group) plants were assessed using laser- induced fluorescence imaging (LIFI). The plants were measured monthly and the evolution of the bacteria on inoculated plants was monitored by RT-qPCR (real-time quantitative PCR) amplification of CLas sequences. A laser emitting at 470 nm was used to excite the fluorescence of chlorophyll and other metabolites present on the leaf. Each image was associated to histograms of colours (colourgrams) and a matrix. For the investigations of data, principal components analysis were made for the categories of the colourgram (scales of red, blue and green, luminosity, relative red, blue and green, hue, saturation and intensity). Preliminary results showed that there are differences in ... Mostrar Tudo |
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Palavras-Chave: |
Evento; Huanglongbing; Plant disease. |
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Thesagro: |
Bactéria; Doença de Planta; Fruta Cítrica; Greening. |
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Thesaurus Nal: |
Candidatus Liberibacter; Citrus. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/84535/1/Proci-10.00257.pdf
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Marc: |
LEADER 02611nam a2200277 a 4500
001 1881236
005 2013-06-18
008 2010 bl uuuu u00u1 u #d
100 1 $aRUSSO, M. S. T.
245 $aStudy of laser-induced fluorescence imaging in asymptomatic leaves from citrus plants inoculated with candidatus liberibacter.
260 $aCitrus research & technology, Cordeirópolis, v. 31, suplemento, p. 122, 2010. Proceedings of the 18 conference international organization citrus virologists, 2010, Campinas.$c2010
520 $aCitrus greening or huanglongbing (HLB) is a devastating disease that has been causing serious problems to citrus cultivation. Some analytical methods have been reported for diagnosis of this disease, mainly DNA-based techniques of Candidatus Liberibacter species, which are the causal agents. In general, most of these developed methods involve PCR (polymerase chain reaction). However, methods based on PCR cannot be used in large-scale to build disease distribution maps. The visual inspection, usually adopted, can lead to misleading diagnoses, since the symptoms are easily mistaken with others problems and the disease has a long asymptomatic phase. Aiming the development of a new method for early diagnosis of this malady, leaves from inoculated and healthy (control group) plants were assessed using laser- induced fluorescence imaging (LIFI). The plants were measured monthly and the evolution of the bacteria on inoculated plants was monitored by RT-qPCR (real-time quantitative PCR) amplification of CLas sequences. A laser emitting at 470 nm was used to excite the fluorescence of chlorophyll and other metabolites present on the leaf. Each image was associated to histograms of colours (colourgrams) and a matrix. For the investigations of data, principal components analysis were made for the categories of the colourgram (scales of red, blue and green, luminosity, relative red, blue and green, hue, saturation and intensity). Preliminary results showed that there are differences in the images obtained from healthy and inoculated plants, indicating a great potential of the technique to perform early diagnoses in large scale that are economically viable.
650 $aCandidatus Liberibacter
650 $aCitrus
650 $aBactéria
650 $aDoença de Planta
650 $aFruta Cítrica
650 $aGreening
653 $aEvento
653 $aHuanglongbing
653 $aPlant disease
700 1 $aCARDINALI, M.
700 1 $aMILORI, D. M. B. P.
700 1 $aPEREIRA, F. M. V.
700 1 $aMARTINS, P. K.
700 1 $aFREITAS-ASTÚA, J.
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Registro original: |
Embrapa Instrumentação (CNPDIA) |
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| 38. |  | LIMA, G. J. M. M. de; LOPES, L. dos S. Importance of evaluating piglet daily weight gain during the first week after weaning. In: JOINT ANNUAL MEETING, 2011, New Orleans. Abstracts. New Orleans: ADSA/ASA, 2011. p. 581. Publicado no Journal Animal of Science, v. 89, e-suppl. 1 e Journal Dairy Science, v. 94, e-suppl. 1, 2011. Projeto: 11.11.11.111.| Tipo: Resumo em Anais de Congresso |
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