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| Registros recuperados : 39 | |
| 17. |  | BACCA, T.; LIMA, E. R.; PICANÇO, M. C.; GUEDES, R. N. C.; VIANA, J. H. M. Optimum spacing of pheromone traps for monitoring the coffee leaf miner Leucoptera coffeella. Entomologia Experimentalis et Applicata, Dordrecht, v. 119, n. 1, p. 39-45, 2006.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 18. | | BACCA, T.; LIMA, E. R.; PICANÇO, M. C.; GUEDES, R. N. C.; VIANA, J. H. M. Sampling plan for the coffe leaf miner Leucoptera coffeella with sex pheromone traps. Journal of Apllied Entomology, Hamburg, v. 132, n. 6, p. 430-438, 2008.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 19. | | SILVA, R. B.; ZANUNCIO, J. C.; SERRÃO, J. E.; LIMA, E. R.; FIGUEIREDO, M. L. C.; CRUZ, I. Suitability of different artificial diets for development and survival of stages of the predaceous ladybird beetle Eriopis connexa. Phytoparasitica, Bet Dagan, v. 37, p. 115-123, 2009.| Biblioteca(s): Embrapa Milho e Sorgo. |
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| 20. | | MOURA, J. I. L.; CIVIDANES, F. J.; SANTOS, L. P. dos; PIRES, J. L.; LIMA, E. R. Influência das fragrâncias florais do híbrido (caiaué x dendezeiro) na visitação dos polinizadores. Agrotrópica, Ilhéus, v. 25, n. 2, p. 97-102, 2013.| Biblioteca(s): Embrapa Amazônia Ocidental. |
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| Registros recuperados : 39 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Pesca e Aquicultura. |
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Data corrente: |
13/01/2023 |
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Data da última atualização: |
13/02/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
B - 2 |
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Autoria: |
LIMA, E. R.; FREIRE, R. P.; SUZUKI, M. F.; OLIVEIRA, J. E.; YOSIDAKI, V. L.; PERONI, C. N.; SEVILHANO, T.; ZORZETO, M.; TORATI, L. S.; SOARES, C. R. J.; LIMA, I. D. de M.; KRONENBERGER, T.; MALTAROLLO, V. G.; BARTOLINI, P. |
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Afiliação: |
ELIANA ROSA LIMA, IPEN-CNEN; RENAN PASSOS FREIRE, IPEN-CNEN; MIRIAM FUSSAE SUZUKI, IPEN-CNEN; JOÃO EZEQUIEL OLIVEIRA, IPEN-CNEN; VANESSA LUNA YOSIDAKI, IPEN-CNEN; CIBELE NUNES PERONI, IPEN-CNEN; THAÍS SEVILHANO, IPEN-CNEN; MOISÉS ZORZETO, PISCICULTURA RAÇA, MT; LUCAS SIMON TORATI, CNPASA; CARLOS ROBERTO JORGE SOARES, IPEN-CNEN; IGOR DANIEL DE MIRANDA LIMA, UNIVERSIDADE FEDERAL DE MINAS GERAIS; THALES KRONENBERGER, Eberhard Karls University Tübingen, Germany; VINICIUS GONÇALVES MALTAROLLO, UNIVERSIDADE FEDERAL DE MINAS GERAIS; PAOLO BARTOLINI, IPEN-CNEN. |
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Título: |
Isolation and characterization of the Arapaima gigas growth hormone (ag-GH) cDNA and three-dimensional modeling of this hormone in comparison with the human hormone (hGH). |
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Ano de publicação: |
2023 |
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Fonte/Imprenta: |
Biomolecules, v. 13, n. 1, art. 158, 2023. |
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ISSN: |
2218-273X |
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DOI: |
https://doi.org/10.3390/biom13010158 |
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Idioma: |
Inglês |
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Conteúdo: |
In a previous work, the common gonadotrophic hormone a-subunit (ag-GTHa), the ag-FSH b-; and ag-LH b-subunit cDNAs, were isolated and characterized by our research group from A. gigas pituitaries, while a preliminary synthesis of ag-FSH was also carried out in human embryonic kidney 293 (HEK293) cells. In the present work, the cDNA sequence encoding the ag-growth hormone (ag-GH) has also been isolated from the same giant Arapaimidae Amazonian fish. The ag-GH consists of 208 amino acids with a putative 23 amino acid signal peptide and a 185 amino acid mature peptide. The highest identity, based on the amino acid sequences, was found with the Elopiformes (82.0%), followed by Anguilliformes (79.7%) and Acipenseriformes (74.5%). The identity with the corresponding human GH (hGH) amino acid sequence is remarkable (44.8%), and the two disulfide bonds present in both sequences were perfectly conserved. Three-dimensional (3D) models of ag-GH, in comparison with hGH, were generated using the threading modeling method followed by molecular dynamics. Our simulations suggest that the two proteins have similar structural properties without major conformational changes under the simulated conditions, even though they are separated from each other by a >100 Myr evolutionary period (1 Myr = 1 million years). The sequence found will be used for the biotechnological synthesis of ag-GH while the ag-GH cDNA obtained will be utilized for preliminary Gene Therapy studies. |
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Palavras-Chave: |
Growth hormone; In silico sequencing. |
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Thesagro: |
Hormônio Animal; Peixe; Pirarucu. |
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Thesaurus NAL: |
Arapaima gigas; Hormones; Molecular models. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1150987/1/biomolecules-2023.pdf
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Marc: |
LEADER 02661naa a2200397 a 4500
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022 $a2218-273X
024 7 $ahttps://doi.org/10.3390/biom13010158$2DOI
100 1 $aLIMA, E. R.
245 $aIsolation and characterization of the Arapaima gigas growth hormone (ag-GH) cDNA and three-dimensional modeling of this hormone in comparison with the human hormone (hGH).$h[electronic resource]
260 $c2023
520 $aIn a previous work, the common gonadotrophic hormone a-subunit (ag-GTHa), the ag-FSH b-; and ag-LH b-subunit cDNAs, were isolated and characterized by our research group from A. gigas pituitaries, while a preliminary synthesis of ag-FSH was also carried out in human embryonic kidney 293 (HEK293) cells. In the present work, the cDNA sequence encoding the ag-growth hormone (ag-GH) has also been isolated from the same giant Arapaimidae Amazonian fish. The ag-GH consists of 208 amino acids with a putative 23 amino acid signal peptide and a 185 amino acid mature peptide. The highest identity, based on the amino acid sequences, was found with the Elopiformes (82.0%), followed by Anguilliformes (79.7%) and Acipenseriformes (74.5%). The identity with the corresponding human GH (hGH) amino acid sequence is remarkable (44.8%), and the two disulfide bonds present in both sequences were perfectly conserved. Three-dimensional (3D) models of ag-GH, in comparison with hGH, were generated using the threading modeling method followed by molecular dynamics. Our simulations suggest that the two proteins have similar structural properties without major conformational changes under the simulated conditions, even though they are separated from each other by a >100 Myr evolutionary period (1 Myr = 1 million years). The sequence found will be used for the biotechnological synthesis of ag-GH while the ag-GH cDNA obtained will be utilized for preliminary Gene Therapy studies.
650 $aArapaima gigas
650 $aHormones
650 $aMolecular models
650 $aHormônio Animal
650 $aPeixe
650 $aPirarucu
653 $aGrowth hormone
653 $aIn silico sequencing
700 1 $aFREIRE, R. P.
700 1 $aSUZUKI, M. F.
700 1 $aOLIVEIRA, J. E.
700 1 $aYOSIDAKI, V. L.
700 1 $aPERONI, C. N.
700 1 $aSEVILHANO, T.
700 1 $aZORZETO, M.
700 1 $aTORATI, L. S.
700 1 $aSOARES, C. R. J.
700 1 $aLIMA, I. D. de M.
700 1 $aKRONENBERGER, T.
700 1 $aMALTAROLLO, V. G.
700 1 $aBARTOLINI, P.
773 $tBiomolecules$gv. 13, n. 1, art. 158, 2023.
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Embrapa Pesca e Aquicultura (CNPASA) |
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