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| Registros recuperados : 70 | |
| 8. |  | ALBUQUERQUE, T. C. S. de; LIMA, H. E. de; LIMA, A. O.; SILVA, L. M.; SILVA, P. L. Absorção de nitrogênio em acessos de cupuaçuzeiro com diferentes níveis de tolerância a vassoura-de-bruxa em Roraima. In: CONGRESSO BRASILEIRO DE FRUTICULTURA, 25., 2017; REUNIÃO ANUAL DA SOCIEDADE INTERAMERICANA DE HORTICULTURA TROPICAL, 62., 2017. Resumos... Porto Seguro, BA: SIHT, 2017.| Biblioteca(s): Embrapa Roraima. |
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| 9. | | LIMA, A. O. de S.; SCHWARTZ, G.; SILVA, A. R.; LUNZ, A. M.; MELLO, A. H. de; ALBINO, U. B. Adaptabilidade de clones de eucalipto à inoculação de fungos micorrízicos em solo arenoso em São Domingos do Araguaia - PA. Colloquium Agrariae, v. 15, n. 6, p. 1-10, nov./dez. 2019.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 10. | | KMIT, M. C. P.; LIMA, A. O. S.; FREITAS, R. C.; ROMAGNOLI, E. M.; ABDALLA, A. L.; MENDES, R. Exploring the sheep rumen shotgun sequencing for funcional analysis and lignocellulolitic enzyme discovery. In: CONGRESO LATINOAMERICANO DE MICROBIOLOGÍA, 23.; CONGRESO ARGENTINO DE MICROBIOLOGIA, 14.; 2016, Rosario. Libro de Resumenes... Rosario: Asociación Latinoamericana de Microbiología; Asociación Argentina de Microbiología, 2016. Ref. JU-1377.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 11. | | MENDES, R.; KMIT, M. C. P.; LIMA, A. O. S.; FREITAS, R. C.; ROMAGNOLI, E. M.; ABDALLA, A. L. Metagenomic analysis of sheep rumen microbiome for carbohidrate-active genes discovery. In: INTERNATIONAL SYMPOSIUM ON MICROBIAL ECOLOGY, 16., 2016, Montreal. Proceedings... Wageningen: The International Society for Microbial Ecology (ISME), 2016. p. 836.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 12. | | LIMA, A. O. de S.; SCHWARTZ, G.; SILVA, A. R.; LUNZ, A. M.; MELLO, A. H. de; ALBINO, U. B. Efeito de microrganismos indutores de crescimento e adubação química em paricá em solo arenoso. Brazilian Journal of Development, v. 6, n. 11, p. 91670-91683, nov. 2020.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 13. | | OLIVEIRA, K. S. de; LIMA, A. O. de; AFONSO, J.; MELO, A. L. de; REGITANO, L. C. de A. Variantes da região promotora do gene KCNJ11 em bovinos da raça Nelore. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 10., 2018, São Carlos, SP. Anais... São Carlos, SP: Embrapa Instrumentação; Embrapa Pecuária Sudeste, 2018. p. 26. (Embrapa Instrumentação. Documentos, 68). Editores técnicos: Daniel Souza Corrêa, Elaine Cristina Paris, Maria Alice Martins, Paulino Ribeiro Villas Boas, Wilson Tadeu Lopes da Silva.| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 14. | | OLIVEIRA, K. S. de; LIMA, A. O. de; AFONSO, J.; CARDOSO, T. F.; BRUSCADIN, J. J.; REGITANO, L. C. de A. Associação entre variantes cis regulatórias do gene KCNJ11e características econômicas em bovinos Nelore. In: JORNADA CIENTÍFICA DA EMBRAPA SÃO CARLOS, 11., 2019, São Carlos, SP. Anais... São Carlos: Embrapa Pecuária Sudeste: Embrapa Instrumentação, 2019. p.23| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 15. | | SERRA, V.; TIZIOTO, P. C.; OLIVEIRA, P. S. N. de; LIMA, A. O. de; GASPARIN, G.; COUTINHO, L. L.; MOURÃO, G. B.; REGITANO, L. C. de A. Análise de qualidade de RNA para estudo do perfil transcriptômico de animais extremos para eficiência alimentar da raça Nelore. In: JORNADA CIENTÍFICA - EMBRAPA SÃO CARLOS, 5., 2013, São Carlos, SP. Anais... São Carlos, SP: Embrapa Pecuária Sudeste: Embrapa Instrumentação , 2013. p. 12 (Embrapa Pecuária Sudeste. Documentos, 110). Editado por Ana Rita de Araújo nogueira, simone Cristina Méo Nicura| Biblioteca(s): Embrapa Pecuária Sudeste. |
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| 16. | | MOKRY, F. B.; LIMA, A. O. de; URBINATI, I.; TORRES JUNIOR, R. A. de A.; HIGA, R. H.; REGITANO, L. C. de A. Associação de SNPs com características de carcaça em uma população da raça Canchim. In: SIMPÓSIO BRASILEIRO DE MELHORAMENTO ANIMAL, 9., 2012, João Pessoa. Anais... João Pessoa: SBMA, 2012. Não paginado. SBMA 2012.| Biblioteca(s): Embrapa Agricultura Digital; Embrapa Gado de Corte; Embrapa Pecuária Sudeste. |
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| 17. | | LOPES, L. D.; LIMA, A. O. S.; SILVA, L. R. F.; ROMAGNOLI, E. M.; TAKETANI, R. G.; FERREIRA, C; ABDALLA, A. L.; MENDES, R. Identificação de enzimas lignocelulolíticas no microbioma do rúmen de ovinos usando metagenômica shotgun. In: CONGRESSO BRASILEIRO DE MICROBIOLOGIA, 27., 2013, Natal. Anais... Natal: Sociedade Brasileira de Microbiologia, 2013. Resumo 901-1| Biblioteca(s): Embrapa Meio Ambiente. |
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| 18. | | LOPES, L. D.; LIMA, A. O. S.; TAKETANI, R. G.; DARIAS, P.; SILVA, L. R. F.; ROMAGNOLI, E. M.; LOUVANDINI, H.; ABDALLA, A. L.; MENDES, R. Exploring the sheep rumen microbiome for carbohydrate-active enzymes. Antonie van Leeuwenhoek, Amsterdam, v. 108, n. 1, p. 15-30, 2015.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 19. | | SOARES JUNIOR, F. L.; DIAS, A. C. F.; FASANELLA, C. C.; TAKETANI, R. G.; LIMA, A. O. S.; MELO, I. S. de; ANDREOTE, F. D. Endo- and exoglucanase activities in bacteria from mangrove sediment. Brazilian Journal of Microbiology, Piracicaba, v. 44, n. 3, p. 969-976, 2013.| Biblioteca(s): Embrapa Meio Ambiente. |
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| 20. | | PEREIRA, R. M. de A.; ARAUJO FILHO, J. A. de; LIMA, R. V.; LIMA, A. O. N.; ARAUJO, Z. B. de. Estudos fenológicos de algumas espécies lenhosas e herbáceas da caatinga. Revista Ciência Agronômica, Fortaleza, v. 20, n. 1, p. 11-20, jun./dez., 1989.| Biblioteca(s): Embrapa Caprinos e Ovinos. |
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| Registros recuperados : 70 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
23/06/2017 |
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Data da última atualização: |
29/06/2017 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
KMIT, M. C. P.; LIMA, A. O. S.; FREITAS, R. C.; ROMAGNOLI, E. M.; ABDALLA, A. L.; MENDES, R. |
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Afiliação: |
M. C. P. KMIT, ESALQ/USP; A. O. S. LIMA, University of Vale do Itajaí; R. C. FREITAS, University of Vale do Itajaí; E. M. ROMAGNOLI, ESALQ/USP; A. L. ABDALLA, CENA, ESALQ/USP; RODRIGO MENDES, CNPMA. |
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Título: |
Exploring the sheep rumen shotgun sequencing for funcional analysis and lignocellulolitic enzyme discovery. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: CONGRESO LATINOAMERICANO DE MICROBIOLOGÍA, 23.; CONGRESO ARGENTINO DE MICROBIOLOGIA, 14.; 2016, Rosario. Libro de Resumenes... Rosario: Asociación Latinoamericana de Microbiología; Asociación Argentina de Microbiología, 2016. Ref. JU-1377. |
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Idioma: |
Inglês |
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Conteúdo: |
The rumen harbors complex microbial communities which participate in an efficient process to digest plant biomass. This ecosytem represents an untapped source of hydrolytic enzymes with potential application for second?generation biofuel production from lignocellulosic biomass. The search for new lignocellulolytic enzymes in microbial communities naturally evolved in the biomass degradation, in environments such as the rumen, using the exploration of the metagenome, is a promising strategy for the exploration of genes. In this context, this study aimed to describe the functions and explores the potential for lignocellulolitic enzyme in the sheep rumen microbiome. The rumen samples were collected from 6 fistulated animals (Ovis aries), divided into two groups and subjected to two diets: control and sugarcane bagasse, 60 days after the beginning of the experiment. Metagenomic DNA was extracted from the solid rumen contents and sequencing was performed in MiSeq Personal Sequencer platform (Illumina®). We analyzed, 4,68 GB of metagenomic DNA from microbes adherent to plant fiber using MGRAST metagenomics analysis server. The functional annotation was performed at MG?RAST for the total functional profile using the KEGG orthology level 2. The shotgun metagenomic reads of all animals samples was assigned to putative lignocellulolitic enzymes when considering nine protein databases at MG?RAST. The predictive functional profiling of the sheep rumen microbiome revealed that amino acid and carbohydrate metabolism, translation, DNA replication and repair, and membrane transport are dominant functions in the rumen microbiome. This functional pattern was similar across all animals. As expected, carbohydrate metabolism was highly represented in our data set, supporting the importance of the rumen microbiome for fiber degradation. Reads classification using nine databases resulted in 22 lignocellulases. For instance, the TrEMBL representing 76,77% out of a total 933639 protein abundance, followed by SwissProt representing 39,95 %, Seed 20,71%, PATRIC 5,92%, IMG 3,63%, KEEG 3,44%, GenBank 3,13%, RefSeq 3,09%, eggnog 1,48%. Based on Cazy search for glycosyl hydrolase (GH) families, more than 50 GH families were detected . The most abundant enzymes were ??glucosidase (GH1; GH30), Endo?1,4???xylanase (GH5; GH10; GH51) , ??N?arabinofuranosidase (GH7; GH51; GH54), ??galactosidase( GH27; GH31; GH36), Acetylesterase, Cellulase (GH5; GH9, GH7), Cellobiose phosphorylase (GH94), ??mannosidase(GH2; GH5), ??galactosidase(GH1; GH2; GH35). This results showing the sheep rumen microbiome as a promising source of new fibrolytic enzymes. MenosThe rumen harbors complex microbial communities which participate in an efficient process to digest plant biomass. This ecosytem represents an untapped source of hydrolytic enzymes with potential application for second?generation biofuel production from lignocellulosic biomass. The search for new lignocellulolytic enzymes in microbial communities naturally evolved in the biomass degradation, in environments such as the rumen, using the exploration of the metagenome, is a promising strategy for the exploration of genes. In this context, this study aimed to describe the functions and explores the potential for lignocellulolitic enzyme in the sheep rumen microbiome. The rumen samples were collected from 6 fistulated animals (Ovis aries), divided into two groups and subjected to two diets: control and sugarcane bagasse, 60 days after the beginning of the experiment. Metagenomic DNA was extracted from the solid rumen contents and sequencing was performed in MiSeq Personal Sequencer platform (Illumina®). We analyzed, 4,68 GB of metagenomic DNA from microbes adherent to plant fiber using MGRAST metagenomics analysis server. The functional annotation was performed at MG?RAST for the total functional profile using the KEGG orthology level 2. The shotgun metagenomic reads of all animals samples was assigned to putative lignocellulolitic enzymes when considering nine protein databases at MG?RAST. The predictive functional profiling of the sheep rumen microbiome revealed that amino acid... Mostrar Tudo |
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Thesagro: |
Enzima; Rúmen. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/161026/1/2016RA-086.pdf
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Marc: |
LEADER 03439nam a2200193 a 4500
001 2071445
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008 2016 bl uuuu u00u1 u #d
100 1 $aKMIT, M. C. P.
245 $aExploring the sheep rumen shotgun sequencing for funcional analysis and lignocellulolitic enzyme discovery.$h[electronic resource]
260 $aIn: CONGRESO LATINOAMERICANO DE MICROBIOLOGÍA, 23.; CONGRESO ARGENTINO DE MICROBIOLOGIA, 14.; 2016, Rosario. Libro de Resumenes... Rosario: Asociación Latinoamericana de Microbiología; Asociación Argentina de Microbiología, 2016. Ref. JU-1377.$c1377
520 $aThe rumen harbors complex microbial communities which participate in an efficient process to digest plant biomass. This ecosytem represents an untapped source of hydrolytic enzymes with potential application for second?generation biofuel production from lignocellulosic biomass. The search for new lignocellulolytic enzymes in microbial communities naturally evolved in the biomass degradation, in environments such as the rumen, using the exploration of the metagenome, is a promising strategy for the exploration of genes. In this context, this study aimed to describe the functions and explores the potential for lignocellulolitic enzyme in the sheep rumen microbiome. The rumen samples were collected from 6 fistulated animals (Ovis aries), divided into two groups and subjected to two diets: control and sugarcane bagasse, 60 days after the beginning of the experiment. Metagenomic DNA was extracted from the solid rumen contents and sequencing was performed in MiSeq Personal Sequencer platform (Illumina®). We analyzed, 4,68 GB of metagenomic DNA from microbes adherent to plant fiber using MGRAST metagenomics analysis server. The functional annotation was performed at MG?RAST for the total functional profile using the KEGG orthology level 2. The shotgun metagenomic reads of all animals samples was assigned to putative lignocellulolitic enzymes when considering nine protein databases at MG?RAST. The predictive functional profiling of the sheep rumen microbiome revealed that amino acid and carbohydrate metabolism, translation, DNA replication and repair, and membrane transport are dominant functions in the rumen microbiome. This functional pattern was similar across all animals. As expected, carbohydrate metabolism was highly represented in our data set, supporting the importance of the rumen microbiome for fiber degradation. Reads classification using nine databases resulted in 22 lignocellulases. For instance, the TrEMBL representing 76,77% out of a total 933639 protein abundance, followed by SwissProt representing 39,95 %, Seed 20,71%, PATRIC 5,92%, IMG 3,63%, KEEG 3,44%, GenBank 3,13%, RefSeq 3,09%, eggnog 1,48%. Based on Cazy search for glycosyl hydrolase (GH) families, more than 50 GH families were detected . The most abundant enzymes were ??glucosidase (GH1; GH30), Endo?1,4???xylanase (GH5; GH10; GH51) , ??N?arabinofuranosidase (GH7; GH51; GH54), ??galactosidase( GH27; GH31; GH36), Acetylesterase, Cellulase (GH5; GH9, GH7), Cellobiose phosphorylase (GH94), ??mannosidase(GH2; GH5), ??galactosidase(GH1; GH2; GH35). This results showing the sheep rumen microbiome as a promising source of new fibrolytic enzymes.
650 $aEnzima
650 $aRúmen
700 1 $aLIMA, A. O. S.
700 1 $aFREITAS, R. C.
700 1 $aROMAGNOLI, E. M.
700 1 $aABDALLA, A. L.
700 1 $aMENDES, R.
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