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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
17/02/2017 |
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Data da última atualização: |
30/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
GIRARDINI, L. K.; PAIM, D. S.; AUSANI, THAIS C.; LOPES, G. V.; PELLEGRINI, D. C. P.; BRITO, M. A. V. P. e; CARDOSO, M. |
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Afiliação: |
Lilian K. Girardini, UNIV. OESTE DANTA CATARINA; Daniel S. Paim, UFRGS; Thais C. Ausani, UFRGS; Graciela V. Lopes, UFRGS; Debora C.P. Pellegrini, UNIV. FEDERAL DO PAMPA; MARIA APARECIDA V PAIVA E BRITO, CNPGL; Marisa Cardoso, UFRGS. |
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Título: |
Antimicrobial resistance profiles of Staphylococcus aureus clusters on small dairy farms in southern Brazil. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, v. 36, n. 10, p. 951-956, 2016. |
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Idioma: |
Inglês |
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Conteúdo: |
In intensive dairy farming, persistent intramammary infection has been associated with specific Staphylococcus (S.) aureus strains, and these strains may be resistant to antimicrobials. The objective of this study was to evaluate the antimicrobial resistance phenotypes of S. aureus isolates and to assess the distribution and the persistence of clonal groups in small dairy herds of southern Brazil. Milk samples were collected from all lactating cows from 21 dairy farms over a two-year period, totaling 1,060 samples. S. aureus isolates were tested for susceptibility to thirteen antimicrobials using the disk diffusion method. The total DNA of the isolates was subjected to SmaI digestion followed by pulsed-field gel electrophoresis (PFGE). Banding patterns differing by ≤4 bands were considered members of a single PFGE cluster. The frequency of S. aureus isolation ranged from 3.45% to 70.59% among the 17 S. aureus-positive herds. Most S. aureus isolates (87.1%) were susceptible to all antimicrobials; resistance to penicillin (18.2%) was the most frequently observed. The 122 isolates subjected to macrorestriction analysis were classified into 30 PFGE-clusters. Among them, only 10 clusters were intermittent or persistent over the two-year period. The majority (93.6%) of isolates belonging to persistent and intermittent clusters were susceptible to all tested antimicrobials. S. aureus intramammary colonization in small dairy farms of southern Brazil is most frequently caused by sporadic PFGE clusters, although some persistent clusters can arise over time. Both sporadic and persistent isolates were highly susceptible to antimicrobials. MenosIn intensive dairy farming, persistent intramammary infection has been associated with specific Staphylococcus (S.) aureus strains, and these strains may be resistant to antimicrobials. The objective of this study was to evaluate the antimicrobial resistance phenotypes of S. aureus isolates and to assess the distribution and the persistence of clonal groups in small dairy herds of southern Brazil. Milk samples were collected from all lactating cows from 21 dairy farms over a two-year period, totaling 1,060 samples. S. aureus isolates were tested for susceptibility to thirteen antimicrobials using the disk diffusion method. The total DNA of the isolates was subjected to SmaI digestion followed by pulsed-field gel electrophoresis (PFGE). Banding patterns differing by ≤4 bands were considered members of a single PFGE cluster. The frequency of S. aureus isolation ranged from 3.45% to 70.59% among the 17 S. aureus-positive herds. Most S. aureus isolates (87.1%) were susceptible to all antimicrobials; resistance to penicillin (18.2%) was the most frequently observed. The 122 isolates subjected to macrorestriction analysis were classified into 30 PFGE-clusters. Among them, only 10 clusters were intermittent or persistent over the two-year period. The majority (93.6%) of isolates belonging to persistent and intermittent clusters were susceptible to all tested antimicrobials. S. aureus intramammary colonization in small dairy farms of southern Brazil is most frequently caused b... Mostrar Tudo |
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Palavras-Chave: |
MRSA; PFGE clusters. |
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Thesaurus Nal: |
mastitis; methicillin-resistant Staphylococcus aureus. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/156043/1/Cnpgl-2016-PesqVetBras-Antimicrobial.pdf
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Marc: |
LEADER 02411naa a2200241 a 4500
001 2064660
005 2023-01-30
008 2016 bl uuuu u00u1 u #d
100 1 $aGIRARDINI, L. K.
245 $aAntimicrobial resistance profiles of Staphylococcus aureus clusters on small dairy farms in southern Brazil.$h[electronic resource]
260 $c2016
520 $aIn intensive dairy farming, persistent intramammary infection has been associated with specific Staphylococcus (S.) aureus strains, and these strains may be resistant to antimicrobials. The objective of this study was to evaluate the antimicrobial resistance phenotypes of S. aureus isolates and to assess the distribution and the persistence of clonal groups in small dairy herds of southern Brazil. Milk samples were collected from all lactating cows from 21 dairy farms over a two-year period, totaling 1,060 samples. S. aureus isolates were tested for susceptibility to thirteen antimicrobials using the disk diffusion method. The total DNA of the isolates was subjected to SmaI digestion followed by pulsed-field gel electrophoresis (PFGE). Banding patterns differing by ≤4 bands were considered members of a single PFGE cluster. The frequency of S. aureus isolation ranged from 3.45% to 70.59% among the 17 S. aureus-positive herds. Most S. aureus isolates (87.1%) were susceptible to all antimicrobials; resistance to penicillin (18.2%) was the most frequently observed. The 122 isolates subjected to macrorestriction analysis were classified into 30 PFGE-clusters. Among them, only 10 clusters were intermittent or persistent over the two-year period. The majority (93.6%) of isolates belonging to persistent and intermittent clusters were susceptible to all tested antimicrobials. S. aureus intramammary colonization in small dairy farms of southern Brazil is most frequently caused by sporadic PFGE clusters, although some persistent clusters can arise over time. Both sporadic and persistent isolates were highly susceptible to antimicrobials.
650 $amastitis
650 $amethicillin-resistant Staphylococcus aureus
653 $aMRSA
653 $aPFGE clusters
700 1 $aPAIM, D. S.
700 1 $aAUSANI, THAIS C.
700 1 $aLOPES, G. V.
700 1 $aPELLEGRINI, D. C. P.
700 1 $aBRITO, M. A. V. P. e
700 1 $aCARDOSO, M.
773 $tPesquisa Veterinária Brasileira$gv. 36, n. 10, p. 951-956, 2016.
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Registro original: |
Embrapa Gado de Leite (CNPGL) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
01/06/2000 |
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Data da última atualização: |
12/06/2013 |
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Autoria: |
LIMA, A. de A. |
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Afiliação: |
ADELISE DE ALMEIDA LIMA, CNPMF. |
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Título: |
Propagação do maracujazeiro. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Cruz das Almas: Embrapa Mandioca e Fruticultura Tropical, 1999. |
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Páginas: |
2p. |
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Série: |
(Embrapa Mandioca e Fruticultura Tropical. Maracujá em foco, 40). |
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Idioma: |
Português |
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Conteúdo: |
Para o estabelecimento de plantios comerciais a propagação do maracujazeiro é normalmente feita através de sementes. As sementes utilizadas para propagação devem ser retiradas de plantas previamente selecionadas, vigorosas, produtivas, precoce, com bons hábitos de crescimento, resistentes a doenças e pragas, originárias de frutos grandes, maduros e com grandes percetagem de suco boa qualidade. As sementes podem secar no interior do frutos, ou serem colhidas e colocadas em um recipiente de vidro ou louça para fermentar. |
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Palavras-Chave: |
Mracujazeiro; Propagacao; Propagation. |
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Thesagro: |
Maracujá; Passiflora Edulis; Semente. |
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Thesaurus NAL: |
passion fruits; seeds. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01154nam a2200229 a 4500
001 1639418
005 2013-06-12
008 1999 bl uuuu u0uu1 u #d
100 1 $aLIMA, A. de A.
245 $aPropagação do maracujazeiro.
260 $aCruz das Almas: Embrapa Mandioca e Fruticultura Tropical$c1999
300 $a2p.
490 $a(Embrapa Mandioca e Fruticultura Tropical. Maracujá em foco, 40).
520 $aPara o estabelecimento de plantios comerciais a propagação do maracujazeiro é normalmente feita através de sementes. As sementes utilizadas para propagação devem ser retiradas de plantas previamente selecionadas, vigorosas, produtivas, precoce, com bons hábitos de crescimento, resistentes a doenças e pragas, originárias de frutos grandes, maduros e com grandes percetagem de suco boa qualidade. As sementes podem secar no interior do frutos, ou serem colhidas e colocadas em um recipiente de vidro ou louça para fermentar.
650 $apassion fruits
650 $aseeds
650 $aMaracujá
650 $aPassiflora Edulis
650 $aSemente
653 $aMracujazeiro
653 $aPropagacao
653 $aPropagation
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Embrapa Mandioca e Fruticultura (CNPMF) |
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