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Registro Completo |
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Biblioteca(s): |
Embrapa Caprinos e Ovinos. |
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Data corrente: |
20/09/1996 |
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Data da última atualização: |
07/08/2021 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
OLIVEIRA, E. R. de. |
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Afiliação: |
EDERLON RIBEIRO DE OLIVEIRA, CNPC. |
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Título: |
Nutrição de caprinos e ovinos no Nordeste do Brasil. |
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Ano de publicação: |
1990 |
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Fonte/Imprenta: |
In: SIMPÓSIO NORDESTINO DE ALIMENTAÇÃO DE RUMINANTES, 3., 1990, JoÃo Pessoa. Anais... JoÃo Pessoa: UFPB-CCA, 1990. p. 94-107. |
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Idioma: |
Português |
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Conteúdo: |
Introducao. Manejo extensivo; Manipulacao da vegetacao lenhosa; Composicao quimica e consumo de nutrientes; Requerimentos nutricionais de caprinos e ovinos em pastoreio. |
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Palavras-Chave: |
Brasil; Nordeste; Northest. |
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Thesagro: |
Caprino; Nutrição animal; Ovino. |
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Thesaurus Nal: |
Animal Nutrition; Brazil; Goats; Sheep. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 00843nam a2200229 a 4500
001 1514488
005 2021-08-07
008 1990 bl uuuu u01u1 u #d
100 1 $aOLIVEIRA, E. R. de
245 $aNutrição de caprinos e ovinos no Nordeste do Brasil.$h[electronic resource]
260 $aIn: SIMPÓSIO NORDESTINO DE ALIMENTAÇÃO DE RUMINANTES, 3., 1990, JoÃo Pessoa. Anais... JoÃo Pessoa: UFPB-CCA, 1990. p. 94-107.$c1990
520 $aIntroducao. Manejo extensivo; Manipulacao da vegetacao lenhosa; Composicao quimica e consumo de nutrientes; Requerimentos nutricionais de caprinos e ovinos em pastoreio.
650 $aAnimal Nutrition
650 $aBrazil
650 $aGoats
650 $aSheep
650 $aCaprino
650 $aNutrição animal
650 $aOvino
653 $aBrasil
653 $aNordeste
653 $aNorthest
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Registro original: |
Embrapa Caprinos e Ovinos (CNPC) |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia; Embrapa Rondônia. |
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Data corrente: |
05/06/2020 |
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Data da última atualização: |
15/09/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
RODRIGUES, S. A. D.; PONTELO, T. P.; KUSSANO, N. R.; KAWAMOTO, T. S.; LEME, L. O.; CAIXETA, F. M. C.; PFEIFER, L. F. M.; FRANCO, M. M.; DODE, M. A. N. |
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Afiliação: |
SARAH A. D. RODRIGUES, Universidade de Brasília; THAIS P. PONTELO, Universidade Federal de Lavras; NAYARA R. KUSSANO, Universidade de Brasília; TAYNAN S. KAWAMOTO, Universidade Federal de Uberlândia; LIGIANE O. LEME, Universidade Federal do Espírito Santo; FELIPPE M. C. CAIXETA, Universidade de Brasília; LUIZ FRANCISCO MACHADO PFEIFER, CPAF-RO; MAURICIO MACHAIM FRANCO, Cenargen; MARGOT ALVES NUNES DODE, Cenargen. |
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Título: |
Effects of Prostaglandins E2 and F2alpha on the in vitro maturation of bovine oocytes. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Domestic Animal Endocrinology, v. 72, article 106447, 2020. |
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ISSN: |
0739-7240 |
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DOI: |
https://doi.org/10.1016/j.domaniend.2020.106447 |
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Idioma: |
Inglês |
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Conteúdo: |
We aimed to elucidate the effects of PGE2 and PGF2a on the in vitro maturation (IVM) of bovine oocytes. First, cumulus-oocyte complexes were matured in the media supplemented with or without PGE2, PGF2a, or PGE2 plus PGF2a for the final 24, 12, or 6 h of culture. Then, the cumulus-oocyte complexes were matured in the absence or presence of a PG endoperoxide synthase 2 (PTGS2) enzyme inhibitor (NS398) supplemented with PGE2, PGF2a, or PGE2 plus PGF2a. Finally, the expression of genes associated with PGs activity in cumulus cells (PTGS2, PG E-synthase-1 [PTGES1], and aldo-keto reductase 1 [AKR1B1]) or oocytes (receptors for PGE2 [PTGER2] and PGF2a [PTGFR]) of different competencies was quantified. Supplementation of the IVM medium with PGs did not improve in vitro embryo production or embryo quality (P > 0.05). During maturation, the relative abundance of PTGS2 transcripts increased (P < 0.05) only in the less-competent group, whereas those of PTGES1 increased in the less-competent and in the more-competent groups. Conversely, AKR1B1 expression decreased only in the less-competent group (P < 0.05). Receptors for the PGE2 and PGF2a genes were very low or undetectable in oocytes. In conclusion, PGE2 and PGF2a are not recommended for media supplementation during maturation because they have no effect on embryo development. Although genes related to PGs activity are differentially expressed in cumulus cells of cumulus-oocyte complexes of different competence during maturation, the expression of PGE2 and PGF2a receptor genes was either not detectable or was detected at low levels in oocytes. MenosWe aimed to elucidate the effects of PGE2 and PGF2a on the in vitro maturation (IVM) of bovine oocytes. First, cumulus-oocyte complexes were matured in the media supplemented with or without PGE2, PGF2a, or PGE2 plus PGF2a for the final 24, 12, or 6 h of culture. Then, the cumulus-oocyte complexes were matured in the absence or presence of a PG endoperoxide synthase 2 (PTGS2) enzyme inhibitor (NS398) supplemented with PGE2, PGF2a, or PGE2 plus PGF2a. Finally, the expression of genes associated with PGs activity in cumulus cells (PTGS2, PG E-synthase-1 [PTGES1], and aldo-keto reductase 1 [AKR1B1]) or oocytes (receptors for PGE2 [PTGER2] and PGF2a [PTGFR]) of different competencies was quantified. Supplementation of the IVM medium with PGs did not improve in vitro embryo production or embryo quality (P > 0.05). During maturation, the relative abundance of PTGS2 transcripts increased (P < 0.05) only in the less-competent group, whereas those of PTGES1 increased in the less-competent and in the more-competent groups. Conversely, AKR1B1 expression decreased only in the less-competent group (P < 0.05). Receptors for the PGE2 and PGF2a genes were very low or undetectable in oocytes. In conclusion, PGE2 and PGF2a are not recommended for media supplementation during maturation because they have no effect on embryo development. Although genes related to PGs activity are differentially expressed in cumulus cells of cumulus-oocyte complexes of different competence during maturation, the... Mostrar Tudo |
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Palavras-Chave: |
E2; Embryonic development; F2a; Maturation; Oócito; Oocyte competence. |
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Thesagro: |
Bovinocultura; Cultura In Vitro; Embriogénese; Maturação; Ovulo; Prostaglandina; Reprodução Animal. |
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Thesaurus NAL: |
Animal reproduction; Cattle; Embryogenesis; Gene expression; In vitro studies; Oocytes; Prostaglandins. |
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Categoria do assunto: |
--
K Ciência Florestal e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/219006/1/cpafro-18481.pdf
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Marc: |
LEADER 02942naa a2200481 a 4500
001 2127907
005 2021-09-15
008 2020 bl uuuu u00u1 u #d
022 $a0739-7240
024 7 $ahttps://doi.org/10.1016/j.domaniend.2020.106447$2DOI
100 1 $aRODRIGUES, S. A. D.
245 $aEffects of Prostaglandins E2 and F2alpha on the in vitro maturation of bovine oocytes.$h[electronic resource]
260 $c2020
520 $aWe aimed to elucidate the effects of PGE2 and PGF2a on the in vitro maturation (IVM) of bovine oocytes. First, cumulus-oocyte complexes were matured in the media supplemented with or without PGE2, PGF2a, or PGE2 plus PGF2a for the final 24, 12, or 6 h of culture. Then, the cumulus-oocyte complexes were matured in the absence or presence of a PG endoperoxide synthase 2 (PTGS2) enzyme inhibitor (NS398) supplemented with PGE2, PGF2a, or PGE2 plus PGF2a. Finally, the expression of genes associated with PGs activity in cumulus cells (PTGS2, PG E-synthase-1 [PTGES1], and aldo-keto reductase 1 [AKR1B1]) or oocytes (receptors for PGE2 [PTGER2] and PGF2a [PTGFR]) of different competencies was quantified. Supplementation of the IVM medium with PGs did not improve in vitro embryo production or embryo quality (P > 0.05). During maturation, the relative abundance of PTGS2 transcripts increased (P < 0.05) only in the less-competent group, whereas those of PTGES1 increased in the less-competent and in the more-competent groups. Conversely, AKR1B1 expression decreased only in the less-competent group (P < 0.05). Receptors for the PGE2 and PGF2a genes were very low or undetectable in oocytes. In conclusion, PGE2 and PGF2a are not recommended for media supplementation during maturation because they have no effect on embryo development. Although genes related to PGs activity are differentially expressed in cumulus cells of cumulus-oocyte complexes of different competence during maturation, the expression of PGE2 and PGF2a receptor genes was either not detectable or was detected at low levels in oocytes.
650 $aAnimal reproduction
650 $aCattle
650 $aEmbryogenesis
650 $aGene expression
650 $aIn vitro studies
650 $aOocytes
650 $aProstaglandins
650 $aBovinocultura
650 $aCultura In Vitro
650 $aEmbriogénese
650 $aMaturação
650 $aOvulo
650 $aProstaglandina
650 $aReprodução Animal
653 $aE2
653 $aEmbryonic development
653 $aF2a
653 $aMaturation
653 $aOócito
653 $aOocyte competence
700 1 $aPONTELO, T. P.
700 1 $aKUSSANO, N. R.
700 1 $aKAWAMOTO, T. S.
700 1 $aLEME, L. O.
700 1 $aCAIXETA, F. M. C.
700 1 $aPFEIFER, L. F. M.
700 1 $aFRANCO, M. M.
700 1 $aDODE, M. A. N.
773 $tDomestic Animal Endocrinology$gv. 72, article 106447, 2020.
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Registro original: |
Embrapa Rondônia (CPAF-RO) |
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