| |
|
|
| Registros recuperados : 82 | |
| 21. |  | PIRES, L. S.; SILVA, M. L. N.; CURI, N.; LEITE, F. P.; BRITO, L. de F. Erosão hídrica pós-plantio em florestas de eucalipto na região centro-leste de Minas Gerais. Pesquisa Agropecuária Brasileira, Brasília, DF, v. 41, n. 4, p. 687-695, abr. 2006 Título em inglês: Water erosion in post-planting eucalyptus forests at center-east region of Minas Gerais State, Brazil.| Biblioteca(s): Embrapa Unidades Centrais. |
|    |
| 23. | | SANTOS JUNIOR, A. G.; CUNHA, R. C.; ANDREOTTI, R.; LEITE, F. P. L. Avaliação de diferentes métodos de purificação da proteína bm86-cg expressa em pichia pastoris. In: CONGRESSO BRASILEIRO DE PARASITOLOGIA VETERINÁRIA, 16., 2010, Campo Grande, MS. [Anais...]. Campo Grande, MS: Colégio Brasileiro de Parasitologia Veterinária, 2010. 1 CD-ROM. 1 p.| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 25. | | OLIVEIRA, F. P. de; SILVA, M. L. N.; AVANZI, J. C.; CURI, N.; LEITE, F. P. Avaliação de perdas de solo em estradas florestais não pavimentadas no Vale do Rio Doce, Minas Gerais. Scientia Forestalis, Piracicaba, v. 38, n. 87, p. 331-339, set. 2010.| Biblioteca(s): Embrapa Florestas. |
| |
| 27. | | SOUZA, C. C.; XAVIER, A.; LEITE, F. P.; SANTANA, R. C.; PAIVA, A. N. de. Densidade de minicepas em minijardim clonal na produção de mudas de eucalipto. Pesquisa Florestal Brasileira, Colombo, v. 34, n. 77, p. 49-56, jan./mar. 2014.| Biblioteca(s): Embrapa Florestas. |
| |
| 28. | | SILVA, G. G. C. da; NEVES, J. C. L.; ALVAREZ, V. H.; LEITE, F. P. Nutritional diagnosis for eucalypt by DRIS, M-DRIS, and CNS. Scientia Agricola, Piracicaba, v. 61, n. 5, p. 507-515, Sept./Oct. 2004.| Biblioteca(s): Embrapa Florestas. |
| |
| 32. | | LEITE, F. P.; BARROS, N. F. de; SANS, L. M. A.; NOVAIS, R. F. de; FABRES, A. S. Regime de água do solo sob eucalipto, mata nativa e pastagem, na região de Guanhaes - MG. IN: CONGRESSO BRASILEIRO DE CIÊNÇIA DO SOLO, 25., 1995, Viçosa, MG. Resumos expandidos. Viçosa: UFV, 1995, v.2, p. 851-853.| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
| |
| 33. | | LEITE, F. P.; BARROS, N. F. de; SANS, L. M. A.; NOVAIS, R. F. de; FABRES, A. S. Regime de água do solo sob eucalipto, mata nativa e pastagem, na região de Guanhães - MG. In: CONGRESSO BRASILEIRO DE CIÊNCIA DO SOLO, 25., 1995, Viçosa, MG. O solo nos grandes domínios morfoclimáticos do Brasil e o desenvolvimento sustentado: resumos expandidos. Viçosa, MG: Sociedade Brasileira de Ciência do Solo: Universidade Federal de Viçosa, 1995. p. 851-853.| Biblioteca(s): Embrapa Milho e Sorgo. |
| |
| 34. | | PINHEIRO, A. F.; MADRI, I. M.; BORSUK, S.; ANDREOTTI, R.; BERME, M. E. A.; LEITE, F. P. L. The adjuvant immune modulation effect in experimental vaccine against Neospora caninum using rNP43 expressed in Pichia pastoris. In: INTERNATIONAL MEETING ON APICOMPLEXAN PARASITES IN FARM ANIMALS, 2012, Lisbon. Apicomplexa in farm animals: proceedings. Lisboa: Escola Superior de Tecnologia da Saúde de Lisboa , 2012. p.131 Poster 66, Apicowplexa2012| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 35. | | PINHEIRO, A. F.; ROLOFF, B. C.; MOREIRA, A. da S.; BERNE, M. E. A.; ANDREOTTI, R.; LEITE, F. P. L. Identification of suitable adjuvant for vaccine formulation with the Neospora caninum antigen NcSRS2. Vaccine, v. 36, n. 9, p. 1154-1159, February 2018| Biblioteca(s): Embrapa Gado de Corte. |
| |
| 39. | | RODY, Y. P.; RIBEIRO, A.; PEZZOPANE, J. E. M.; GLERIANI, J. M.; ALMEIDA, A. Q.; LEITE, F. P. Estimates of the leaf area index (lai) using lai-2000 and hemispherical photos in Eucalyptus plantations. Ciência Florestal, Santa Maria, v. 24, n. 4, p. 925-934, out./dez. 2014.| Biblioteca(s): Embrapa Florestas. |
| |
| 40. | | BRITO, L. de F.; SILVA, M. L. N.; LEITE, F. P.; FERREIRA, M. M.; PIRES, L. S. Erosão hídrica de latossolo vermelho muito argiloso relevo ondulado em área de pós-plantio de eucalipto no Vale do Rio Doce, região Centro Leste do Estado de Minas Gerais. Scientia Forestalis, Piracicaba, n. 67, p. 27-36, abr. 2005.| Biblioteca(s): Embrapa Florestas. |
| |
| Registros recuperados : 82 | |
|
|
|
Registro Completo
|
Biblioteca(s): |
Embrapa Gado de Corte. |
|
Data corrente: |
18/12/2018 |
|
Data da última atualização: |
18/12/2018 |
|
Tipo da produção científica: |
Artigo em Periódico Indexado |
|
Circulação/Nível: |
A - 1 |
|
Autoria: |
PINHEIRO, A. F.; ROLOFF, B. C.; MOREIRA, A. da S.; BERNE, M. E. A.; ANDREOTTI, R.; LEITE, F. P. L. |
|
Afiliação: |
Amanda Fernandes Pinheiro, Departamento de Microbiologia e Parasitologia/Instituto de Biologia/Universidade Federal de Pelotas - UFPel; Bárbara Couto Roloff, Núcleo de Tecnologia/Centro de Desenvolvimento Tecnológico/Universidade Federal de Pelotas - UFPel; Angelita da Silveira Moreira, Núcleo de Tecnologia/Centro de Desenvolvimento Tecnológico/Universidade Federal de Pelotas - UFPel; Maria Elisabeth Aires Berne, Departamento de Microbiologia e Parasitologia/Instituto de Biologia/Universidade Federal de Pelotas - UFPel; RENATO ANDREOTTI E SILVA, CNPGC; Fábio Pereira Leivas Leite, Departamento de Microbiologia e Parasitologia/Instituto de Biologia/Universidade Federal de Pelotas – UFPel. |
|
Título: |
Identification of suitable adjuvant for vaccine formulation with the Neospora caninum antigen NcSRS2. |
|
Ano de publicação: |
2018 |
|
Fonte/Imprenta: |
Vaccine, v. 36, n. 9, p. 1154-1159, February 2018 |
|
Idioma: |
Inglês |
|
Conteúdo: |
The parasite Neospora caninum is the main cause of abortion in cattle in many countries around the world, so a vaccine is a rational approach method for the control of the disease. An effective vaccine should be able to prevent both, the horizontal and vertical transmission of N. caninum. In this study, the immune vaccinal response of the recombinant protein rNcSRS2 of N. caninum expressed in Pichia pastoris and formulated with water-in-oil emulsion, xanthan gum, and alum hydroxide was assessed in an experimental murine model. Groups of 10 Balb/c mice were subcutaneously inoculated with two doses of prNcSRS2 twenty-one days apart. After the second immunization, four mice from each group were euthanized, and splenocytes were stimulated ex vivo with recombinant protein. The IgG dynamics were evaluated by indirect ELISA, and the splenocytes cytokines transcription by qPCR. All groups elicited specific antibodies against prNcSRS2, with the water-in-oil group showing significantly (p .05) elevated titers compared to the other groups. The prNcSRS2 protein alone did not induce a significant ex vivo splenic transcription level of IFN-c, TNF-a, IL-4, IL-10, and IL-12 cytokines, except for IL-17A, and the adjuvant associations with the prNcSRS2 protein induced different cytokine transcription profiles. The water-inoil emulsion modulated the expression of TNF-a; the xanthan gum modulated IL-4, IL-10, and IL-12; and alum hydroxide modulated IFN-c, TNF-a, IL-4, IL-10, and IL-12. In conclusion, it was found that the association of the recombinant prNcSRS2 protein with different adjuvants induced different levels of specific antibody, and a distinct splenic cytokine profile in an adjuvant-dependent manner. The mechanisms of adjuvancity activity is complex, so adjuvant formulation may help in the design of efficient vaccine to control Neosporosis. MenosThe parasite Neospora caninum is the main cause of abortion in cattle in many countries around the world, so a vaccine is a rational approach method for the control of the disease. An effective vaccine should be able to prevent both, the horizontal and vertical transmission of N. caninum. In this study, the immune vaccinal response of the recombinant protein rNcSRS2 of N. caninum expressed in Pichia pastoris and formulated with water-in-oil emulsion, xanthan gum, and alum hydroxide was assessed in an experimental murine model. Groups of 10 Balb/c mice were subcutaneously inoculated with two doses of prNcSRS2 twenty-one days apart. After the second immunization, four mice from each group were euthanized, and splenocytes were stimulated ex vivo with recombinant protein. The IgG dynamics were evaluated by indirect ELISA, and the splenocytes cytokines transcription by qPCR. All groups elicited specific antibodies against prNcSRS2, with the water-in-oil group showing significantly (p .05) elevated titers compared to the other groups. The prNcSRS2 protein alone did not induce a significant ex vivo splenic transcription level of IFN-c, TNF-a, IL-4, IL-10, and IL-12 cytokines, except for IL-17A, and the adjuvant associations with the prNcSRS2 protein induced different cytokine transcription profiles. The water-inoil emulsion modulated the expression of TNF-a; the xanthan gum modulated IL-4, IL-10, and IL-12; and alum hydroxide modulated IFN-c, TNF-a, IL-4, IL-10, and IL-12. In concl... Mostrar Tudo |
|
Thesagro: |
Neospora Caninum. |
|
Thesaurus NAL: |
Adjuvants; Cytokines; Proteins; Vaccines. |
|
Categoria do assunto: |
-- |
|
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/188808/1/Identification-of-suitable-adjuvant-for-vaccine.pdf
|
|
Marc: |
LEADER 02555naa a2200241 a 4500
001 2101958
005 2018-12-18
008 2018 bl uuuu u00u1 u #d
100 1 $aPINHEIRO, A. F.
245 $aIdentification of suitable adjuvant for vaccine formulation with the Neospora caninum antigen NcSRS2.$h[electronic resource]
260 $c2018
520 $aThe parasite Neospora caninum is the main cause of abortion in cattle in many countries around the world, so a vaccine is a rational approach method for the control of the disease. An effective vaccine should be able to prevent both, the horizontal and vertical transmission of N. caninum. In this study, the immune vaccinal response of the recombinant protein rNcSRS2 of N. caninum expressed in Pichia pastoris and formulated with water-in-oil emulsion, xanthan gum, and alum hydroxide was assessed in an experimental murine model. Groups of 10 Balb/c mice were subcutaneously inoculated with two doses of prNcSRS2 twenty-one days apart. After the second immunization, four mice from each group were euthanized, and splenocytes were stimulated ex vivo with recombinant protein. The IgG dynamics were evaluated by indirect ELISA, and the splenocytes cytokines transcription by qPCR. All groups elicited specific antibodies against prNcSRS2, with the water-in-oil group showing significantly (p .05) elevated titers compared to the other groups. The prNcSRS2 protein alone did not induce a significant ex vivo splenic transcription level of IFN-c, TNF-a, IL-4, IL-10, and IL-12 cytokines, except for IL-17A, and the adjuvant associations with the prNcSRS2 protein induced different cytokine transcription profiles. The water-inoil emulsion modulated the expression of TNF-a; the xanthan gum modulated IL-4, IL-10, and IL-12; and alum hydroxide modulated IFN-c, TNF-a, IL-4, IL-10, and IL-12. In conclusion, it was found that the association of the recombinant prNcSRS2 protein with different adjuvants induced different levels of specific antibody, and a distinct splenic cytokine profile in an adjuvant-dependent manner. The mechanisms of adjuvancity activity is complex, so adjuvant formulation may help in the design of efficient vaccine to control Neosporosis.
650 $aAdjuvants
650 $aCytokines
650 $aProteins
650 $aVaccines
650 $aNeospora Caninum
700 1 $aROLOFF, B. C.
700 1 $aMOREIRA, A. da S.
700 1 $aBERNE, M. E. A.
700 1 $aANDREOTTI, R.
700 1 $aLEITE, F. P. L.
773 $tVaccine$gv. 36, n. 9, p. 1154-1159, February 2018
Download
Esconder MarcMostrar Marc Completo |
|
Registro original: |
Embrapa Gado de Corte (CNPGC) |
|
|
Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
Fechar
|
| Nenhum registro encontrado para a expressão de busca informada. |
|
|
