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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
23/01/2017 |
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Data da última atualização: |
23/01/2017 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CALVI, G. P.; AUD, F. F.; FERRAZ, I. D. K.; PRITCHARD, H. W.; KRANNER, I. |
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Afiliação: |
G. P. CALVI, INPA; FABIANA FERRAZ AUD, CNPMF; I. D. K. FERRAZ, INPA; H. W. PRITCHARD, Royal Botanic Gardens; I. KRANNER, Royal Botanic Gardens. |
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Título: |
Analyses of several seed viability markers in individual recalcitrant seeds of Eugenia stipitata McVaugh with totipotent germination. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Plant Biology, n. 19, p. 6-13, German Botanical Society; d The Royal Botanical Society of the Netherlands, 2017. |
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ISSN: |
1435-8603 |
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Idioma: |
Inglês |
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Conteúdo: |
The use of biochemical seed viability markers is often compromised by the unknown partitioning of analytes in bulk seed lots consisting of inseparable populations of viable and nonviable seeds. We took advantage of an unusual morphological syndrome found in the recalcitrant, undifferentiated seeds of Eugenia stipitata: one seed can be cut into several parts, each of which can germinate and develop into seedlings. We used four seed parts from one individual seed to analyse seed moisture content (MC), seed viability and the antioxidant glutathione (c-glutamyl-cysteinyl-glycine; GSH), glutathione disulphide (GSSG) and intermediates of glutathione synthesis and breakdown. Seeds were exposed to different environmental MC to induce various levels of desiccation stress. Upon storage at high seed MC, seed viability was maintained, while GSH concentration increased and the glutathione half-cell reduction potential (EGSSG/2GSH) was less negative than 215 mV, indicating GSH production and highly reducing conditions. Storage at low seed MC led to loss of GSH, resulting in a shift in EGSSG/2GSH, and seed death. In contrast, the cyst(e)ine half-cell reduction potential (ECySS/2CYS) could not distinguish between the viability categories. Previous studies on seed populations revealed that the probability for a seed being alive is 50% at EGSSG/2GSH values between 180 and 160 mV. The single seed approach revealed that the window in which seed viability was lost could be slightly shifted towards more negative values. We discuss the contribution of cellular pH to EGSSG/2GSH and recommend E. stipitata as a recalcitrant seed model to study stress response on a single seed basis. MenosThe use of biochemical seed viability markers is often compromised by the unknown partitioning of analytes in bulk seed lots consisting of inseparable populations of viable and nonviable seeds. We took advantage of an unusual morphological syndrome found in the recalcitrant, undifferentiated seeds of Eugenia stipitata: one seed can be cut into several parts, each of which can germinate and develop into seedlings. We used four seed parts from one individual seed to analyse seed moisture content (MC), seed viability and the antioxidant glutathione (c-glutamyl-cysteinyl-glycine; GSH), glutathione disulphide (GSSG) and intermediates of glutathione synthesis and breakdown. Seeds were exposed to different environmental MC to induce various levels of desiccation stress. Upon storage at high seed MC, seed viability was maintained, while GSH concentration increased and the glutathione half-cell reduction potential (EGSSG/2GSH) was less negative than 215 mV, indicating GSH production and highly reducing conditions. Storage at low seed MC led to loss of GSH, resulting in a shift in EGSSG/2GSH, and seed death. In contrast, the cyst(e)ine half-cell reduction potential (ECySS/2CYS) could not distinguish between the viability categories. Previous studies on seed populations revealed that the probability for a seed being alive is 50% at EGSSG/2GSH values between 180 and 160 mV. The single seed approach revealed that the window in which seed viability was lost could be slightly shifted towar... Mostrar Tudo |
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Thesagro: |
Semente. |
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Categoria do assunto: |
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Marc: |
LEADER 02347naa a2200193 a 4500
001 2061470
005 2017-01-23
008 2017 bl uuuu u00u1 u #d
022 $a1435-8603
100 1 $aCALVI, G. P.
245 $aAnalyses of several seed viability markers in individual recalcitrant seeds of Eugenia stipitata McVaugh with totipotent germination.$h[electronic resource]
260 $c2017
520 $aThe use of biochemical seed viability markers is often compromised by the unknown partitioning of analytes in bulk seed lots consisting of inseparable populations of viable and nonviable seeds. We took advantage of an unusual morphological syndrome found in the recalcitrant, undifferentiated seeds of Eugenia stipitata: one seed can be cut into several parts, each of which can germinate and develop into seedlings. We used four seed parts from one individual seed to analyse seed moisture content (MC), seed viability and the antioxidant glutathione (c-glutamyl-cysteinyl-glycine; GSH), glutathione disulphide (GSSG) and intermediates of glutathione synthesis and breakdown. Seeds were exposed to different environmental MC to induce various levels of desiccation stress. Upon storage at high seed MC, seed viability was maintained, while GSH concentration increased and the glutathione half-cell reduction potential (EGSSG/2GSH) was less negative than 215 mV, indicating GSH production and highly reducing conditions. Storage at low seed MC led to loss of GSH, resulting in a shift in EGSSG/2GSH, and seed death. In contrast, the cyst(e)ine half-cell reduction potential (ECySS/2CYS) could not distinguish between the viability categories. Previous studies on seed populations revealed that the probability for a seed being alive is 50% at EGSSG/2GSH values between 180 and 160 mV. The single seed approach revealed that the window in which seed viability was lost could be slightly shifted towards more negative values. We discuss the contribution of cellular pH to EGSSG/2GSH and recommend E. stipitata as a recalcitrant seed model to study stress response on a single seed basis.
650 $aSemente
700 1 $aAUD, F. F.
700 1 $aFERRAZ, I. D. K.
700 1 $aPRITCHARD, H. W.
700 1 $aKRANNER, I.
773 $tPlant Biology$gn. 19, p. 6-13, German Botanical Society; d The Royal Botanical Society of the Netherlands, 2017.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
ID |
Origem |
Tipo/Formato |
Classificação |
Cutter |
Registro |
Volume |
Status |
URL |
Voltar
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Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
09/08/2007 |
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Data da última atualização: |
06/01/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
Internacional - A |
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Autoria: |
STEINER, C.; TEIXEIRA, W. G.; LEHMANN, J.; NEHLS, T.; MACÊDO, J. L. V. de; BLUM, W. E. H.; ZECH, W. |
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Afiliação: |
Christoph Steiner, University of Bayreuth; Wenceslau Geraldes Teixeira, CPAA; Johannes Lehmann, Cornell University; Thomas Nehls, University of Bayreuth; Jeferson Luis Vasconcelos de Macêdo, CPAA; Winfried E.H. Blum, University of Natural Resources and Applied Life Sciences; Wolfgang Zech, University of Bayreuth. |
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Título: |
Long term effects of manure, charcoal and mineral fertilization on crop production and fertility on a highly weathered Central Amazonian upland soil. |
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Ano de publicação: |
2007 |
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Fonte/Imprenta: |
Plant and Soil, v. 291, n. 1-2, p. 275-290, 2007. |
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Idioma: |
Inglês |
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Conteúdo: |
Objectives: (1) quantify the effects of charcoal, organic, and inorganic fertilization on soil fertility and crop production; and (2) to evaluate the sustainability of charcoal additions in terms of maintaning high-SOM contents and nutrient availability with special emphasis on Terra firme Ferrasols near Manaus. |
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Palavras-Chave: |
Terra preta de índio. |
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Thesagro: |
Agricultura Orgânica; Fertilidade; Solo. |
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Thesaurus NAL: |
Amazonia. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/114931/1/art3A10.10072Fs11104-007-9193-9.pdf
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Marc: |
LEADER 01062naa a2200253 a 4500
001 1680986
005 2015-01-06
008 2007 bl uuuu u00u1 u #d
100 1 $aSTEINER, C.
245 $aLong term effects of manure, charcoal and mineral fertilization on crop production and fertility on a highly weathered Central Amazonian upland soil.
260 $c2007
520 $aObjectives: (1) quantify the effects of charcoal, organic, and inorganic fertilization on soil fertility and crop production; and (2) to evaluate the sustainability of charcoal additions in terms of maintaning high-SOM contents and nutrient availability with special emphasis on Terra firme Ferrasols near Manaus.
650 $aAmazonia
650 $aAgricultura Orgânica
650 $aFertilidade
650 $aSolo
653 $aTerra preta de índio
700 1 $aTEIXEIRA, W. G.
700 1 $aLEHMANN, J.
700 1 $aNEHLS, T.
700 1 $aMACÊDO, J. L. V. de
700 1 $aBLUM, W. E. H.
700 1 $aZECH, W.
773 $tPlant and Soil$gv. 291, n. 1-2, p. 275-290, 2007.
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Embrapa Amazônia Ocidental (CPAA) |
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