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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
18/03/2019 |
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Data da última atualização: |
19/12/2023 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
SILVA, P. L. R.; MACEDO, L. L. P. de; SÁ, M. E. L. de; AMORIM. R. M. S. de; MORGANTE, C. V.; TESSUTTI, I. T. L.; BASSO, M. F.; GALBIERI, R.; SÁ, M. F. G. de. |
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Afiliação: |
PAOLO L. RODRIGUES SILVA; LEONARDO L. P. DE MACEDO; M. EUGENIA LISEI DE SÁ; REGINA, M. S. DE AMORIM; CAROLINA VIANNA MORGANTE, CPATSA; ISABELA T. LOURENÇO TESSUTTI; MARCOS F. BASSO; RAFAEL GALBIERI; MARIA F. GROSSI DE SÁ. |
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Título: |
Transgenic cotton applied to phytonematode control using in plant RNA interfering strategy. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
In: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec, 2018. |
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Idioma: |
Inglês |
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Conteúdo: |
Cotton (Gossypium hirsutum) is one of the most important commodities worldwide, and it is among the ten largest sources of wealth in the Brazilian agribusiness. However, its productivity is constantly threatened by biotic and abiotic factors. Plant-parasitic nematodes (PPN) are one of the most damaging pathogens, representing global losses of approximately US$ 100 bi/annual. More and more, biotechnology tools have contributed to overcoming these constraints through the development of tolerant or resistant plants. Interfering RNA (RNAi) technology has been successfully exploited in different plant species for achieving resistance against different pathogens. The cross-talk of these engineered RNAi produced in planthas been shown to be an efficient strategy to knockdown essential genes in PPN. Previous studies of our research team have identified several potential molecules from nematodes involved in nematode-plant interaction. The Mi-1, Mi-2 and Mi-3 genes are, respectively, an avirulence protein, a cysteine protease involved in plant parasitism, and a protein involved in the formation of egg mass matrix. Here, cotton GM plants constitutively overexpressing a hairpin-derived dsRNA targeting simultaneously these three genes of Meloidogyne incognita were obtained from biolistic-transformation. Transgenic plants were in vitro and ex vitro (leaf-painting) selected using herbicide Imazapyr and QuickStix kit for LibertyLink® (bar gene).Twelve PCR-positive events were characterized and plants from T1 and T2 generation were challenged with M. incognita, using root gall index and number of eggs to evaluate plant resistance/susceptibility. Data will be significant to the nematode management in cotton crops. MenosCotton (Gossypium hirsutum) is one of the most important commodities worldwide, and it is among the ten largest sources of wealth in the Brazilian agribusiness. However, its productivity is constantly threatened by biotic and abiotic factors. Plant-parasitic nematodes (PPN) are one of the most damaging pathogens, representing global losses of approximately US$ 100 bi/annual. More and more, biotechnology tools have contributed to overcoming these constraints through the development of tolerant or resistant plants. Interfering RNA (RNAi) technology has been successfully exploited in different plant species for achieving resistance against different pathogens. The cross-talk of these engineered RNAi produced in planthas been shown to be an efficient strategy to knockdown essential genes in PPN. Previous studies of our research team have identified several potential molecules from nematodes involved in nematode-plant interaction. The Mi-1, Mi-2 and Mi-3 genes are, respectively, an avirulence protein, a cysteine protease involved in plant parasitism, and a protein involved in the formation of egg mass matrix. Here, cotton GM plants constitutively overexpressing a hairpin-derived dsRNA targeting simultaneously these three genes of Meloidogyne incognita were obtained from biolistic-transformation. Transgenic plants were in vitro and ex vitro (leaf-painting) selected using herbicide Imazapyr and QuickStix kit for LibertyLink® (bar gene).Twelve PCR-positive events were characterized ... Mostrar Tudo |
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Palavras-Chave: |
Algodão transgenico; Fitonematode; Interferência de RNA; Knockdown de genes; Nemátodos parasitas de plantas. |
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Thesagro: |
Algodão; Gossypium Hirsutum; Nematóide. |
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Thesaurus Nal: |
Cotton. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/194386/1/Morgante.pdf
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Marc: |
LEADER 02755nam a2200313 a 4500
001 2107167
005 2023-12-19
008 2018 bl uuuu u00u1 u #d
100 1 $aSILVA, P. L. R.
245 $aTransgenic cotton applied to phytonematode control using in plant RNA interfering strategy.$h[electronic resource]
260 $aIn: BRAZILIAN BIOTECHNOLOGY CONGRESS, 7.; BIOTECHNOLOGY IBERO-AMERICAN CONGRESS, 2., 2018, Brasília, DF. Proceedings... Brasília, DF: SBBiotec$c2018
520 $aCotton (Gossypium hirsutum) is one of the most important commodities worldwide, and it is among the ten largest sources of wealth in the Brazilian agribusiness. However, its productivity is constantly threatened by biotic and abiotic factors. Plant-parasitic nematodes (PPN) are one of the most damaging pathogens, representing global losses of approximately US$ 100 bi/annual. More and more, biotechnology tools have contributed to overcoming these constraints through the development of tolerant or resistant plants. Interfering RNA (RNAi) technology has been successfully exploited in different plant species for achieving resistance against different pathogens. The cross-talk of these engineered RNAi produced in planthas been shown to be an efficient strategy to knockdown essential genes in PPN. Previous studies of our research team have identified several potential molecules from nematodes involved in nematode-plant interaction. The Mi-1, Mi-2 and Mi-3 genes are, respectively, an avirulence protein, a cysteine protease involved in plant parasitism, and a protein involved in the formation of egg mass matrix. Here, cotton GM plants constitutively overexpressing a hairpin-derived dsRNA targeting simultaneously these three genes of Meloidogyne incognita were obtained from biolistic-transformation. Transgenic plants were in vitro and ex vitro (leaf-painting) selected using herbicide Imazapyr and QuickStix kit for LibertyLink® (bar gene).Twelve PCR-positive events were characterized and plants from T1 and T2 generation were challenged with M. incognita, using root gall index and number of eggs to evaluate plant resistance/susceptibility. Data will be significant to the nematode management in cotton crops.
650 $aCotton
650 $aAlgodão
650 $aGossypium Hirsutum
650 $aNematóide
653 $aAlgodão transgenico
653 $aFitonematode
653 $aInterferência de RNA
653 $aKnockdown de genes
653 $aNemátodos parasitas de plantas
700 1 $aMACEDO, L. L. P. de
700 1 $aSÁ, M. E. L. de
700 1 $aAMORIM. R. M. S. de
700 1 $aMORGANTE, C. V.
700 1 $aTESSUTTI, I. T. L.
700 1 $aBASSO, M. F.
700 1 $aGALBIERI, R.
700 1 $aSÁ, M. F. G. de
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Registro original: |
Embrapa Semiárido (CPATSA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Tabuleiros Costeiros. Para informações adicionais entre em contato com cpatc.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Tabuleiros Costeiros. |
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Data corrente: |
29/02/1996 |
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Data da última atualização: |
29/02/1996 |
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Autoria: |
LEAL, E. C.; SANTOS, Z. G. dos; RAM, C.; WARWICK, D. R. N.; LEAL, M. L. da S.; RENARD, J. L. |
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Título: |
Efeito da adubacao mineral sobre a incidencia das lixas Sphaerodothis torrendiella e Sphaerodothis acrocomiae no coqueiro Cocos nucifera L. |
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Ano de publicação: |
1994 |
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Fonte/Imprenta: |
Oleagineux, v.49, n.5, p.213-220, 1994. |
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Idioma: |
Inglês |
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Conteúdo: |
As observacoes foram realizadas durante dois anos, no experimento de adubacao mineral isntalado em 1984, no municipio de Santa Luzia do Itanhy - Sergipe, Brasil para determinar o efeito do N,P,K e Mg sobre a incidencia das lixas pequenas e grande do coqueiro Cocos nucifera L. causadas por Sphaerodothis torrendiella e S. acrocomiae, respectivamente. |
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Palavras-Chave: |
Coconut; Coqueiro; Diseases; Doencas; Efeito; Lixa grande; Lixa pequena; Nutricao mineral; Sphaerodothis acrocomiae; Sphaerodothis torrendiella. |
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Thesagro: |
Cocos Nucifera. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 01232naa a2200313 a 4500
001 1353676
005 1996-02-29
008 1994 bl uuuu u00u1 u #d
100 1 $aLEAL, E. C.
245 $aEfeito da adubacao mineral sobre a incidencia das lixas Sphaerodothis torrendiella e Sphaerodothis acrocomiae no coqueiro Cocos nucifera L.
260 $c1994
520 $aAs observacoes foram realizadas durante dois anos, no experimento de adubacao mineral isntalado em 1984, no municipio de Santa Luzia do Itanhy - Sergipe, Brasil para determinar o efeito do N,P,K e Mg sobre a incidencia das lixas pequenas e grande do coqueiro Cocos nucifera L. causadas por Sphaerodothis torrendiella e S. acrocomiae, respectivamente.
650 $aCocos Nucifera
653 $aCoconut
653 $aCoqueiro
653 $aDiseases
653 $aDoencas
653 $aEfeito
653 $aLixa grande
653 $aLixa pequena
653 $aNutricao mineral
653 $aSphaerodothis acrocomiae
653 $aSphaerodothis torrendiella
700 1 $aSANTOS, Z. G. dos
700 1 $aRAM, C.
700 1 $aWARWICK, D. R. N.
700 1 $aLEAL, M. L. da S.
700 1 $aRENARD, J. L.
773 $tOleagineux$gv.49, n.5, p.213-220, 1994.
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