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Registro Completo |
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
19/03/2013 |
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Data da última atualização: |
19/03/2013 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
BARBOSA-FERREIRA, M; VALLE, C. B. do; BRUM, K. B.; OLIVEIRA, N. M. R.; FERREIRA, V. B. N.; GARCEZ, V. S.; RIET-CORREA, F.; LEMOS, R. A. A. de. |
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Afiliação: |
MARCOS BARBOSA-FERREIRA, Professor, Universidade Anhanguera-Uniderp R. Alexandre Herculano, 1400, Jardim Veraneio, Campo Grande MS; CACILDA BORGES DO VALLE, CNPGC; KARINE BONUCIELLI BRUM, UFMS, Centro de Ciências Biológicas e da Saúde; Nara Mírcea Rodrigues Oliveira, Faculdade de Medicina Veterinária da UFMS, bolsista de Iniciação Científica CNPq.; VALQUIRIA BARBOSA NANTES FERREIRA, Bolsista de Desenvolvimento Tecnológico Industrial do INCT, Faculdade de Química, UFMS, Campo Grande, MS.; VALMIR SILVA GARCEZ, Faculdade de Química, UFMS, Campo Grande, MS; FLANKLIN RIET-CORREA, Hospital Veterinário, CSTR, UFCG, Patos, PB; RICARDO ANTONIO AMARAL DE LEMOS, Faculdade de Medicina Veterinária e Zootecnia, UFMS, Campo Grande, MS. |
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Título: |
Antinutritional factors in plants. Steroidal saponin (Protodioscin) in Brachiraria spp. Preliminary data. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: INTERNATIONAL SYMPOSIUM ON FORAGE BREEDING, 3., 2011, Bonito, MS. Breeding forages for climate change adaptation and mitigation - eco-efficient animal production: proceedings. [Campo Grande, MS: Embrapa Gado de Corte], 2011. 1 CD-ROM. III SIMF. p 322-332. |
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Páginas: |
11 p. |
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Descrição Física: |
1 CD-ROM |
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Idioma: |
Português |
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Notas: |
III SIMF |
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Conteúdo: |
Outbreaks of hepatogenous photosensitization have been reported in ruminants grazing Brachiraria spp. Saponins are secondary metabolites present in that grass, and these substances are
related as toxic causing the disease. The objective of this study was to identify and quantify the variations of the saponin protodioscin content in green leaves of different Brachiraria
species/ecotypes. Samples of leaves were collected from 10 ecotypes of Brachiraria spp., from August 2009 to August 2010. Following the procedure of extracting saponins from the dried and
crushed leaves, the analysis was performed using HPLC-ELSD for identification and quantification of protodioscin. It was observed that the species B. decumbens has the highest concentration of
protodioscin (2.33% ± 0.54), B. brizantha var. Arapoty has the highest concentration (1.24% ± 0.44) between the B. brizantha ecotypes. Finally B. humidicola cultivars have the lowest concentrations ranging from 0.11 to 0.9%. These data confirm the observations of other researchers who cite B. decumbens as responsible for most cases of hepatogenous photosensitization. It can be concluded that the varieties of B. decumbens are those with the highest concentrations of protodioscin, followed by varieties of B. brizantha and varieties of B. humidicola. |
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Palavras-Chave: |
Hepatic disease; Secondary metabolite. |
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Thesaurus Nal: |
poisoning; sheep. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 02303naa a2200277 a 4500
001 1953404
005 2013-03-19
008 2011 bl uuuu u00u1 u #d
100 1 $aBARBOSA-FERREIRA, M
245 $aAntinutritional factors in plants. Steroidal saponin (Protodioscin) in Brachiraria spp. Preliminary data.
260 $c2011
300 $a11 p.$c1 CD-ROM
500 $aIII SIMF
520 $aOutbreaks of hepatogenous photosensitization have been reported in ruminants grazing Brachiraria spp. Saponins are secondary metabolites present in that grass, and these substances are related as toxic causing the disease. The objective of this study was to identify and quantify the variations of the saponin protodioscin content in green leaves of different Brachiraria species/ecotypes. Samples of leaves were collected from 10 ecotypes of Brachiraria spp., from August 2009 to August 2010. Following the procedure of extracting saponins from the dried and crushed leaves, the analysis was performed using HPLC-ELSD for identification and quantification of protodioscin. It was observed that the species B. decumbens has the highest concentration of protodioscin (2.33% ± 0.54), B. brizantha var. Arapoty has the highest concentration (1.24% ± 0.44) between the B. brizantha ecotypes. Finally B. humidicola cultivars have the lowest concentrations ranging from 0.11 to 0.9%. These data confirm the observations of other researchers who cite B. decumbens as responsible for most cases of hepatogenous photosensitization. It can be concluded that the varieties of B. decumbens are those with the highest concentrations of protodioscin, followed by varieties of B. brizantha and varieties of B. humidicola.
650 $apoisoning
650 $asheep
653 $aHepatic disease
653 $aSecondary metabolite
700 1 $aVALLE, C. B. do
700 1 $aBRUM, K. B.
700 1 $aOLIVEIRA, N. M. R.
700 1 $aFERREIRA, V. B. N.
700 1 $aGARCEZ, V. S.
700 1 $aRIET-CORREA, F.
700 1 $aLEMOS, R. A. A. de
773 $tIn: INTERNATIONAL SYMPOSIUM ON FORAGE BREEDING, 3., 2011, Bonito, MS. Breeding forages for climate change adaptation and mitigation - eco-efficient animal production: proceedings. [Campo Grande, MS: Embrapa Gado de Corte], 2011. 1 CD-ROM. III SIMF. p 322-332.
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Registro original: |
Embrapa Gado de Corte (CNPGC) |
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
28/06/2017 |
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Data da última atualização: |
29/12/2017 |
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Autoria: |
CAMPOS, L. L.; LANDIM-ALVARENGA, F. C.; IKEDA, T. L.; MONTEIRO, B. A.; MAIA, L.; FREITAS-Dell'AQUA, C. P.; De VITA, B. |
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Afiliação: |
Loreta L. Campos, Departamento de Reprodução Animal e Radiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp); Fernanda C. Landim-Alvarenga, Departamento de Reprodução Animal e Radiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp); Tatícia L. Ikeda, Departamento de Reprodução Animal e Radiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp); Bianca A. Monteiro, Departamento de Reprodução Animal e Radiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp); Leandro Maia, Departamento de Reprodução Animal e Radiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp); Camila P. Freitas-Dell?Aqua, Departamento de Reprodução Animal e Radiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp); Bruna De Vita, Departamento de Reprodução Animal e Radiologia Veterinária, Faculdade de Medicina Veterinária e Zootecnia, Universidade Estadual Paulista Júlio de Mesquita Filho (Unesp). |
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Título: |
Isolation, culture, characterization and cryopreservation of stem cells derived from amniotic mesenchymal layer and umbilical cord tissue of bovine fetuses. |
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Ano de publicação: |
2017 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 37, n. 3, p. 278-286, março 2017. |
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Idioma: |
Inglês |
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Notas: |
Título em português: Isolamento, cultura, caracterização e criopreservação de células tronco derivadas de camada amniótica mesenquimal e de tecido do cordão umbilical de fetuses bovinos. |
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Conteúdo: |
Stem cells are undifferentiated cells with a high proliferation potential. These cells canbe characterized by their in vivo ability to self-renew and to differentiate into specialized cell lines. The most used stem cell types, in both human and veterinary fields, are the mesenchymal stem cells (MSC) derived from bone marrow and adipose tissue. Nowadays, there is a great interest in using stem cells derived from fetal tissues, such as amniotic membrane (AM) and umbilical cord tissue (UCT), which can be obtained non-invasively at delivery time. Due to the scarcity of studies in bovine species, the aim of this study was to isolate, characterize, differentiate and cryopreserve MSC derived from the mesenchymal layer of amniotic membrane (AM), for the first time, and umbilical cord tissue (UCT) of dairy cow neonates after assisted delivery (AD) and from fetus at initial third of pregnancy (IT) obtained in slaughterhouse. Cells were isolated by enzymatic digestion of the tissue fragments with 0.1% collagenase solution. Six samples of AM and UCT at delivery time and six samples of AM and UCT at first trimester of pregnancy were subjected to morphology evaluation, imunophenotype characterization, in vitro osteogenic, adipogenic and chondrogenic differentiation and viability analysis after cryopreservation. All samples showed adherence to plastic and fibroblast-like morphology. Immunocytochemistry revealed expression of CD 44, NANOG and OCT-4 and lack of expression of MHC II in MSC from all samples. Flow cytometry demonstrated that cells from all samples expressed CD 44, did not or low expressed CD 34 (AM: IT-0.3%a, AD-3.4%b; UCT: 0.4%, 1.4%) and MHC II (AM: IT-1.05%a, AD-9.7%b; UCT: IT-0.7%a, AD-5.7%b). They were also capable of trilineage mesenchymal differentiation and showed 80% viability after cryopreservation. According to the results, bovine AM and UCT-derived cells, either obtained at delivery time or from slaughterhouse, are a painless and non-invasive source of MSC and can be used for stem cell banking. MenosStem cells are undifferentiated cells with a high proliferation potential. These cells canbe characterized by their in vivo ability to self-renew and to differentiate into specialized cell lines. The most used stem cell types, in both human and veterinary fields, are the mesenchymal stem cells (MSC) derived from bone marrow and adipose tissue. Nowadays, there is a great interest in using stem cells derived from fetal tissues, such as amniotic membrane (AM) and umbilical cord tissue (UCT), which can be obtained non-invasively at delivery time. Due to the scarcity of studies in bovine species, the aim of this study was to isolate, characterize, differentiate and cryopreserve MSC derived from the mesenchymal layer of amniotic membrane (AM), for the first time, and umbilical cord tissue (UCT) of dairy cow neonates after assisted delivery (AD) and from fetus at initial third of pregnancy (IT) obtained in slaughterhouse. Cells were isolated by enzymatic digestion of the tissue fragments with 0.1% collagenase solution. Six samples of AM and UCT at delivery time and six samples of AM and UCT at first trimester of pregnancy were subjected to morphology evaluation, imunophenotype characterization, in vitro osteogenic, adipogenic and chondrogenic differentiation and viability analysis after cryopreservation. All samples showed adherence to plastic and fibroblast-like morphology. Immunocytochemistry revealed expression of CD 44, NANOG and OCT-4 and lack of expression of MHC II in MSC fr... Mostrar Tudo |
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Palavras-Chave: |
Anexo fetal; Cell banking; Célula tronco mesenquimal; Fetal annexes; Holstein cow; Immunophetotype; Imunofenótipo; MHC class II; Vaca holandesa. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/161149/1/Isolation-culture-characterization.pdf
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Marc: |
LEADER 03217naa a2200313 a 4500
001 2071637
005 2017-12-29
008 2017 bl uuuu u00u1 u #d
100 1 $aCAMPOS, L. L.
245 $aIsolation, culture, characterization and cryopreservation of stem cells derived from amniotic mesenchymal layer and umbilical cord tissue of bovine fetuses.$h[electronic resource]
260 $c2017
500 $aTítulo em português: Isolamento, cultura, caracterização e criopreservação de células tronco derivadas de camada amniótica mesenquimal e de tecido do cordão umbilical de fetuses bovinos.
520 $aStem cells are undifferentiated cells with a high proliferation potential. These cells canbe characterized by their in vivo ability to self-renew and to differentiate into specialized cell lines. The most used stem cell types, in both human and veterinary fields, are the mesenchymal stem cells (MSC) derived from bone marrow and adipose tissue. Nowadays, there is a great interest in using stem cells derived from fetal tissues, such as amniotic membrane (AM) and umbilical cord tissue (UCT), which can be obtained non-invasively at delivery time. Due to the scarcity of studies in bovine species, the aim of this study was to isolate, characterize, differentiate and cryopreserve MSC derived from the mesenchymal layer of amniotic membrane (AM), for the first time, and umbilical cord tissue (UCT) of dairy cow neonates after assisted delivery (AD) and from fetus at initial third of pregnancy (IT) obtained in slaughterhouse. Cells were isolated by enzymatic digestion of the tissue fragments with 0.1% collagenase solution. Six samples of AM and UCT at delivery time and six samples of AM and UCT at first trimester of pregnancy were subjected to morphology evaluation, imunophenotype characterization, in vitro osteogenic, adipogenic and chondrogenic differentiation and viability analysis after cryopreservation. All samples showed adherence to plastic and fibroblast-like morphology. Immunocytochemistry revealed expression of CD 44, NANOG and OCT-4 and lack of expression of MHC II in MSC from all samples. Flow cytometry demonstrated that cells from all samples expressed CD 44, did not or low expressed CD 34 (AM: IT-0.3%a, AD-3.4%b; UCT: 0.4%, 1.4%) and MHC II (AM: IT-1.05%a, AD-9.7%b; UCT: IT-0.7%a, AD-5.7%b). They were also capable of trilineage mesenchymal differentiation and showed 80% viability after cryopreservation. According to the results, bovine AM and UCT-derived cells, either obtained at delivery time or from slaughterhouse, are a painless and non-invasive source of MSC and can be used for stem cell banking.
653 $aAnexo fetal
653 $aCell banking
653 $aCélula tronco mesenquimal
653 $aFetal annexes
653 $aHolstein cow
653 $aImmunophetotype
653 $aImunofenótipo
653 $aMHC class II
653 $aVaca holandesa
700 1 $aLANDIM-ALVARENGA, F. C.
700 1 $aIKEDA, T. L.
700 1 $aMONTEIRO, B. A.
700 1 $aMAIA, L.
700 1 $aFREITAS-Dell'AQUA, C. P.
700 1 $aDe VITA, B.
773 $tPesquisa Veterinária Brasileira, Rio de Janeiro$gv. 37, n. 3, p. 278-286, março 2017.
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