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| Registros recuperados : 71 | |
| 8. |  | FIGUEIREDO, J. F.; PELLEGRIN, A. O.; LEITE, K. M.; LAGE, A. P. Comparacao entre diferentes preparacoes de antigenos de Campylobacter fetus subso. venerelais para utilizacao em um teste de ELISA IgA. In: CONGRESSO BRASILEIRO DE VETERIONARIA, 27.; CONGRESSO PAULISTA DE VETERINARIA, 5.; CONFERENCIA ANUAL DA SOCIEDADE PAULISTA DE MEDICINA VETERINARIA, 55.; EXPOVET, 6., 2000, Aguas de Lindoia. Resumos. Aguas de Lindoia: [s.n.], 2000. p.88.| Biblioteca(s): Embrapa Pantanal. |
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| 9. | | MIRANDA, K. L.; LAGE, A. P.; MACHADO, R. P.; FIGUEIREDO, J. F.; PELLEGRIN, A. O. Comparacao entre diferentes preparacoes de antigenos de Campylobacter fetus venerealis para utilizacao em um teste de ELISA IgA. In: SEMANA DO CONHECIMENTO DA UFMG, 1.; SEMANA DE INICIACAO CIENTIFICA, 9., 2000, Belo Horizonte. Resumos. Belo Horizonte: UFMG, 2000. p.30.| Biblioteca(s): Embrapa Pantanal. |
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| 10. | | PELEGRIN, A. O.; FIGUEIREDO, J. F.; BARBOSA, E. F.; LEITE, R. C.; LAGE, A. P. Immunoblotting para deteccao de imunoglobulina A anti-Campylobacter fetus em muco cervico-vaginal de femeas bovinas. In: CONGRESSO BRASILEIRO DE VETERIONARIA, 27.; CONGRESSO PAULISTA DE VETERINARIA, 5.; CONFERENCIA ANUAL DA SOCIEDADE PAULISTA DE MEDICINA VETERINARIA, 55.; EXPOVET, 6., 2000, Aguas de Lindoia. Resumos. Aguas de Lindoia: [s.n.], 2000. p.78.| Biblioteca(s): Embrapa Pantanal. |
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| 17. | | FÓSCOLO, C. B.; PELLEGRIN, A. O.; LEITE, R. C.; STYNEN, A. P. R.; LAGE, A. P. Vaccination of bulls against bovine genital campylobacteriosis: a therapeutic approach. Animal Reproduction, v. 2, n. 2, p.122-127, apr./jun., 2005.| Biblioteca(s): Embrapa Pantanal. |
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| 18. | | PELLEGRIN, A. O.; LEITE, R. C.; LAGE, A. P.; FIGUEIREDO, J.; STANCIOLI, E. F. B.; SERENO, J. R. B. Ensaio imunoenzimatico e "imunoblotting" para deteccao de imunoglobulinas a anti- Campylobacter fetus em muco cervico- vaginal de femeas bovinas. In: SEMANA DA POS-GRADUACAO DA UFMG, 3., 2002, Belo Horizonte. Anais... Belo Horizonte: UFMG, 2002. nao paginado.| Biblioteca(s): Embrapa Pantanal. |
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| 19. | | PELLEGRIN, A. O.; BARBOSA, E. F.; REINATO, A. P. R.; LEITE, R. C.; RAVAGLIA, E.; LAGE, A. P. Padronizacao de um ensaio imunoenzimatico para deteccao de IgA anti-Campylobacter fetus em muco cervico-vaginal de femeas bovinas. In: ENCONTRO DE PESQUISA DA ESCOLA DE VETERINARIA, 16., 1998, Belo Horizonte. Anais... Belo Horizonte: UFMG-Escola de Veterinaria, 1998. p.85| Biblioteca(s): Embrapa Pantanal. |
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| 20. | | RODRIGUES, R. O.; HERRMANN, G. P.; HEINEMANN, M. B.; LAGE, A. P.; LOPES, L. B.; MOREIRA, E. C. Comparação entre a imunidade induzida em bovinos vacinados com bacterinas polivalentes comerciais e uma monovalente experimental. Pesquisa Veterinária Brasileira, Rio de Janeiro, v. 31, n. 1, p. 10-16, 2011.| Biblioteca(s): Embrapa Agrossilvipastoril. |
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| Registros recuperados : 71 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
18/08/2021 |
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Data da última atualização: |
19/08/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
PEREZ, V. K. C.; CUSTODIO, D. A. C.; SILVA, E. M. M.; OLIVEIRA, J. de; GUIMARÃES, A. S.; BRITO, M. A. V. P.; SOUZA-FILHO, A. F.; HEINEMANN, M. B.; LAGE, A. P.; DORNELES, E. M. S. |
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Afiliação: |
VERÓNICA K. C. PÉREZ, Universidade Federal de Lavras, Lavras; DIRCÉIA A. C. CUSTÓDIO, Universidade Federal de Lavras; EDUARDA M. M. SILVA, Universidade Federal de Lavras; JULIA DE OLIVEIRA, Universidade Federal de Lavras; ALESSANDRO DE SA GUIMARAES, CNPGL; MARIA A. V. P. BRITO; ANTÔNIO F. SOUZA-FILHO, Universidade de São Paulo; MARCOS B. HEINEMANN, Universidade de São Paulo; ANDREY P. LAGE, Universidade Federal de Minas Gerais; ELAINE M. S. DORNELES, Universidade Federal de Lavras. |
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Título: |
Virulence factors and antimicrobial resistance in Staphylococcus aureus isolated from bovine mastitis in Brazil. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Brazilian Journal of Microbiology, v. 51, p. 2111-2122, 2020. |
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DOI: |
https://doi.org/10.1007/s42770-020-00363-5 |
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Idioma: |
Inglês |
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Conteúdo: |
This study aimed to evaluate virulence factors and genetic markers of antimicrobial resistance in 400 Staphylococcus aureus strains isolated from bovine mastitis in four Brazilian states, as well as to assess the association between these characteristics and field information. Virulence factors and drug resistance genes were identified by PCR screening. Biofilm-forming and hemolytic phenotype were detected using Congo red Tryptic Soy Broth and defibrinated sheep blood agar, respectively. Of all isolates, 83.5% were biofilm-forming and 98.5% strains exhibited biofilm gene icaAD, and a significant association between phenotype and genotype for biofilm was observed (P = 0.0005). Hemolysin genes were observed in 82.85% (hla+hlb+), 16.5% (hla+) and 0.75% (hlb+) isolates, whereas the hemolytic phenotype exhibited was complete and incomplete hemolysis in 64.25%, complete in 28.25%, incomplete in 4.75%, and negative in 2.75% of the strains. Virulence factors genes luk, seb, sec, sed, and tst were observed in 3.5%, 0.5%, 1%, 0.25%, and 0.74% isolates, respectively. The gene blaZ was detected in 82.03% of penicillin-resistant isolates, whereas tetK and aac(6′)-Ie?aph(2′)-Ia were observed in 33.87% and 45.15% of the tetracycline and aminoglycosides-resistant isolates, respectively. Fluoroquinolone resistance gene mepA was detected for the first time in S. aureus from bovine mastitis. Resistance genes tetM (3.22%), tetL (1.61%), ermA (14.29%), ermB (14.29%), ermC (33.3%), ermT (9.52%), ermY (4.76%), msrA (9.52%), and mphC (9.52%) were also detected among resistant isolates. No association between virulence factors or antimicrobialresistant genes and year of isolation, geographic origin, or antimicrobial resistance profile was observed. Our results showed that S. aureus strains isolated from bovine mastitis in the four Brazilian states sampled are mainly biofilmforming and hemolytic, whereas virulence genes associated with enterotoxins, luk and tst, were less frequently observed. Moreover, a wide variety of resistance genes that confer resistance to almost all classes of antimicrobial agents approved for use in animals and humans were found. Overall, the data point to a great pathogenic potential of S. aureus associated with bovine mastitis and to the non-negligible risks to public health of staphylococcal infections from animal origin. MenosThis study aimed to evaluate virulence factors and genetic markers of antimicrobial resistance in 400 Staphylococcus aureus strains isolated from bovine mastitis in four Brazilian states, as well as to assess the association between these characteristics and field information. Virulence factors and drug resistance genes were identified by PCR screening. Biofilm-forming and hemolytic phenotype were detected using Congo red Tryptic Soy Broth and defibrinated sheep blood agar, respectively. Of all isolates, 83.5% were biofilm-forming and 98.5% strains exhibited biofilm gene icaAD, and a significant association between phenotype and genotype for biofilm was observed (P = 0.0005). Hemolysin genes were observed in 82.85% (hla+hlb+), 16.5% (hla+) and 0.75% (hlb+) isolates, whereas the hemolytic phenotype exhibited was complete and incomplete hemolysis in 64.25%, complete in 28.25%, incomplete in 4.75%, and negative in 2.75% of the strains. Virulence factors genes luk, seb, sec, sed, and tst were observed in 3.5%, 0.5%, 1%, 0.25%, and 0.74% isolates, respectively. The gene blaZ was detected in 82.03% of penicillin-resistant isolates, whereas tetK and aac(6′)-Ie?aph(2′)-Ia were observed in 33.87% and 45.15% of the tetracycline and aminoglycosides-resistant isolates, respectively. Fluoroquinolone resistance gene mepA was detected for the first time in S. aureus from bovine mastitis. Resistance genes tetM (3.22%), tetL (1.61%), ermA (14.29%), ermB (14.29%), ermC (33.3%), er... Mostrar Tudo |
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Palavras-Chave: |
Hemolisina; Infecção intramamária; Intramammary infection; Mastite; Staphylococci. |
|
Thesagro: |
Biofilme; Bovino; Doença Animal; Estafilococo. |
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Thesaurus NAL: |
Biofilm; Hemolysins. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
|
Marc: |
LEADER 03482naa a2200373 a 4500
001 2133706
005 2021-08-19
008 2020 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s42770-020-00363-5$2DOI
100 1 $aPEREZ, V. K. C.
245 $aVirulence factors and antimicrobial resistance in Staphylococcus aureus isolated from bovine mastitis in Brazil.$h[electronic resource]
260 $c2020
520 $aThis study aimed to evaluate virulence factors and genetic markers of antimicrobial resistance in 400 Staphylococcus aureus strains isolated from bovine mastitis in four Brazilian states, as well as to assess the association between these characteristics and field information. Virulence factors and drug resistance genes were identified by PCR screening. Biofilm-forming and hemolytic phenotype were detected using Congo red Tryptic Soy Broth and defibrinated sheep blood agar, respectively. Of all isolates, 83.5% were biofilm-forming and 98.5% strains exhibited biofilm gene icaAD, and a significant association between phenotype and genotype for biofilm was observed (P = 0.0005). Hemolysin genes were observed in 82.85% (hla+hlb+), 16.5% (hla+) and 0.75% (hlb+) isolates, whereas the hemolytic phenotype exhibited was complete and incomplete hemolysis in 64.25%, complete in 28.25%, incomplete in 4.75%, and negative in 2.75% of the strains. Virulence factors genes luk, seb, sec, sed, and tst were observed in 3.5%, 0.5%, 1%, 0.25%, and 0.74% isolates, respectively. The gene blaZ was detected in 82.03% of penicillin-resistant isolates, whereas tetK and aac(6′)-Ie?aph(2′)-Ia were observed in 33.87% and 45.15% of the tetracycline and aminoglycosides-resistant isolates, respectively. Fluoroquinolone resistance gene mepA was detected for the first time in S. aureus from bovine mastitis. Resistance genes tetM (3.22%), tetL (1.61%), ermA (14.29%), ermB (14.29%), ermC (33.3%), ermT (9.52%), ermY (4.76%), msrA (9.52%), and mphC (9.52%) were also detected among resistant isolates. No association between virulence factors or antimicrobialresistant genes and year of isolation, geographic origin, or antimicrobial resistance profile was observed. Our results showed that S. aureus strains isolated from bovine mastitis in the four Brazilian states sampled are mainly biofilmforming and hemolytic, whereas virulence genes associated with enterotoxins, luk and tst, were less frequently observed. Moreover, a wide variety of resistance genes that confer resistance to almost all classes of antimicrobial agents approved for use in animals and humans were found. Overall, the data point to a great pathogenic potential of S. aureus associated with bovine mastitis and to the non-negligible risks to public health of staphylococcal infections from animal origin.
650 $aBiofilm
650 $aHemolysins
650 $aBiofilme
650 $aBovino
650 $aDoença Animal
650 $aEstafilococo
653 $aHemolisina
653 $aInfecção intramamária
653 $aIntramammary infection
653 $aMastite
653 $aStaphylococci
700 1 $aCUSTODIO, D. A. C.
700 1 $aSILVA, E. M. M.
700 1 $aOLIVEIRA, J. de
700 1 $aGUIMARÃES, A. S.
700 1 $aBRITO, M. A. V. P.
700 1 $aSOUZA-FILHO, A. F.
700 1 $aHEINEMANN, M. B.
700 1 $aLAGE, A. P.
700 1 $aDORNELES, E. M. S.
773 $tBrazilian Journal of Microbiology$gv. 51, p. 2111-2122, 2020.
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