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| Registros recuperados : 4 | |
| 1. |  | LACORTE, G. A.; MACHADO, M. A.; CAMPOS, A. L.; SILVA, R. S. da; CARVALHO, M. R. S.; FONSECA, C. G da. Caracterização do poliformismo DGAT1 VNTR em raças bovinas brasileiras. In: CONGRESSO BRASILEIRO DE GENÉTICA, 53., 2007, Águas de Lindóia. Resumos... Ribeirão Preto: SBG, 2007. p. 123.| Biblioteca(s): Embrapa Gado de Leite. |
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| 2. | | SILVA, R. S. da; PEREIRA, L.; LACORTE, G. A.; MACHADO, M. A.; PEIXOTO, M. G. C. D.; VERNEQUE, R. da S.; CARVALHO, M. R. S. Novo método, simples e econômico para detecção do polimorfismo K232A no gene DGAT1 baseado em tetra-primer ARMS. In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE ZOOTECNIA. 45., 2008, Lavras, MG. Anais... Lavras: SBZ / UFLA, 2008. 1 CD.| Biblioteca(s): Embrapa Gado de Leite. |
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| 3. | | STEINBERG, R. S.; PEREIRA, L.; LACORTE, G. A.; PEIXOTO, M. G. C. D.; VERNEQUE, R. da S.; TEODORO, R. L.; MACHADO, M. A.; FONSECA, C. G.; CARVALHO, M. R. S. A new and cost-effective method for detection of the bovine acyl-CoA:diacylglycerol acyltransferase 1 K232A polymorphism in cattle. Journal of Dairy Science, v. 92, p. 773-776, 2009. 92 Nota Técnica| Biblioteca(s): Embrapa Gado de Leite. |
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| 4. | | STEINBERG, R. S.; PEREIRA, L.; LACORTE, G. A.; PEIXOTO, M. G. C. D.; VERNEQUE, R. da S.; TEODORO, R. L.; MACHADO, M. A.; FONSECA, C. G.; CARVALHO, M. R. S. Technical note: A new and cost-effective method for detection of the bovine acyl-CoA: diacyglycerol acyltransferase 1 K232A polymorphism in cattle. Journal of Dairy Science, v. 92, n. 2, p. 773-776, 2009.| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 4 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
26/04/2010 |
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Data da última atualização: |
16/02/2024 |
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Tipo da produção científica: |
Nota Técnica/Nota Científica |
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Autoria: |
STEINBERG, R. S.; PEREIRA, L.; LACORTE, G. A.; PEIXOTO, M. G. C. D.; VERNEQUE, R. da S.; TEODORO, R. L.; MACHADO, M. A.; FONSECA, C. G.; CARVALHO, M. R. S. |
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Afiliação: |
R. S. STEINBERG, UFMG; L. PEREIRA, UFMG; G. A. LACORTE, UFMG; MARIA GABRIELA CAMPOLINA D PEIXOTO, CNPGL; RUI DA SILVA VERNEQUE, CNPGL; ROBERTO LUIZ TEODORO, Pesquisador aposentado do CNPGL; MARCO ANTONIO MACHADO, CNPGL; C. G. FONSECA, UFMG; M. R. S. CARVALHO, UFMG. |
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Título: |
Technical note: A new and cost-effective method for detection of the bovine acyl-CoA: diacyglycerol acyltransferase 1 K232A polymorphism in cattle. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
Journal of Dairy Science, v. 92, n. 2, p. 773-776, 2009. |
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Idioma: |
Inglês |
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Conteúdo: |
A new, quick, and inexpensive method for detecting the bovine acyl-CoA:diacylglycerol acyltransferase1 (DGAT1) polymorphism (K232A) through tetra-primer amplification refractory mutation system by PCR (ARMS-PCR) was developed in the present investigation. The DGAT1 gene was recently identified as underlying variation in milk production traits. To date, PCR techniques such as PCR-RFLP have been used for detecting the DGAT1 K232A polymorphism, despite being expensive and laborious. The method proposed here, a tetra-primer ARMS-PCR, showed 100% sensitivity and specificity when compared with PCR-RFLP results obtained in a sample of 80 animals tested in a double-blind system. Our results suggest that the use of tetra-primer ARMS-PCR for DGAT1 K232A polymorphism genotyping could greatly reduce costs providing information for both research purposes and for dairy cattle breeders who perform DGAT1 genotyping for gene-assisted selection. |
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Palavras-Chave: |
DGAT1. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/711762/1/Technical-note-A-new-and-cost-effective-method-for-detection-of-the-bovine-acyl-CoA.pdf
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Marc: |
LEADER 01675naa a2200229 a 4500
001 1711762
005 2024-02-16
008 2009 bl uuuu u00u1 u #d
100 1 $aSTEINBERG, R. S.
245 $aTechnical note$bA new and cost-effective method for detection of the bovine acyl-CoA: diacyglycerol acyltransferase 1 K232A polymorphism in cattle.$h[electronic resource]
260 $c2009
520 $aA new, quick, and inexpensive method for detecting the bovine acyl-CoA:diacylglycerol acyltransferase1 (DGAT1) polymorphism (K232A) through tetra-primer amplification refractory mutation system by PCR (ARMS-PCR) was developed in the present investigation. The DGAT1 gene was recently identified as underlying variation in milk production traits. To date, PCR techniques such as PCR-RFLP have been used for detecting the DGAT1 K232A polymorphism, despite being expensive and laborious. The method proposed here, a tetra-primer ARMS-PCR, showed 100% sensitivity and specificity when compared with PCR-RFLP results obtained in a sample of 80 animals tested in a double-blind system. Our results suggest that the use of tetra-primer ARMS-PCR for DGAT1 K232A polymorphism genotyping could greatly reduce costs providing information for both research purposes and for dairy cattle breeders who perform DGAT1 genotyping for gene-assisted selection.
653 $aDGAT1
700 1 $aPEREIRA, L.
700 1 $aLACORTE, G. A.
700 1 $aPEIXOTO, M. G. C. D.
700 1 $aVERNEQUE, R. da S.
700 1 $aTEODORO, R. L.
700 1 $aMACHADO, M. A.
700 1 $aFONSECA, C. G.
700 1 $aCARVALHO, M. R. S.
773 $tJournal of Dairy Science$gv. 92, n. 2, p. 773-776, 2009.
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Embrapa Gado de Leite (CNPGL) |
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