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Registro Completo |
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Biblioteca(s): |
Embrapa Arroz e Feijão. |
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Data corrente: |
16/02/2012 |
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Data da última atualização: |
16/02/2012 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
HERRADA, M. R.; GOMES, J. B.; MOREIRA, R. R. L. de S.; LEANDRO, W. M.; FERREIRA, E. P. de B. |
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Afiliação: |
MARISOL RIVERO HERRADA, doutorando UFG; JORDANA BORGES GOMES, estudante UFG; RANIERI RAMADHAM LINO DE SOUZA MOREIRA, estudante UFG; WILSON MOZENA LEANDRO, UFG; ENDERSON PETRONIO DE BRITO FERREIRA, CNPAF. |
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Título: |
Efeito de leguminosas isoladas e consorciadas com milheto no crescimento de feijoeiro orgânico em plantio direto. |
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Ano de publicação: |
2011 |
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Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 10., 2011, Goiânia. Anais... Goiânia: Embrapa Arroz e Feijão, 2011. |
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Descrição Física: |
1 CD-ROM. |
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Idioma: |
Português |
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Notas: |
CONAFE. |
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Conteúdo: |
O objetivo do presente trabalho foi de avaliar o efeito de leguminosas isoladas e consorciadas com milheto no crescimento do feijoeiro comum orgânico em sistemas de plantio convencional e direto. |
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Thesagro: |
Agricultura orgânica; Feijão; Phaseolus vulgaris; Plantio direto. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/54310/1/MC31.pdf
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Marc: |
LEADER 00946nam a2200229 a 4500
001 1915952
005 2012-02-16
008 2011 bl uuuu u00u1 u #d
100 1 $aHERRADA, M. R.
245 $aEfeito de leguminosas isoladas e consorciadas com milheto no crescimento de feijoeiro orgânico em plantio direto.
260 $aIn: CONGRESSO NACIONAL DE PESQUISA DE FEIJÃO, 10., 2011, Goiânia. Anais... Goiânia: Embrapa Arroz e Feijão$c2011
300 $c1 CD-ROM.
500 $aCONAFE.
520 $aO objetivo do presente trabalho foi de avaliar o efeito de leguminosas isoladas e consorciadas com milheto no crescimento do feijoeiro comum orgânico em sistemas de plantio convencional e direto.
650 $aAgricultura orgânica
650 $aFeijão
650 $aPhaseolus vulgaris
650 $aPlantio direto
700 1 $aGOMES, J. B.
700 1 $aMOREIRA, R. R. L. de S.
700 1 $aLEANDRO, W. M.
700 1 $aFERREIRA, E. P. de B.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Tipo/Formato |
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Cutter |
Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
22/02/2013 |
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Data da última atualização: |
07/03/2023 |
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Tipo da produção científica: |
Artigo em Anais de Congresso |
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Autoria: |
CARVALHO, J. O.; MICHALCZECHEN-LACERDA, V. A.; SARTORI, R.; RODRIGUES, F. C.; BRAVIM, O.; FRANCO, M. M.; DODE, M. A. N. |
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Afiliação: |
JOSE O. CARVALHO, UNIVERSIDADE DE SÃO PAULO; VALQUIRIA A. MICHALCZECHEN-LACERDA, UnB; ROBERTO SARTORI, UNIVERSIDADE DE SÃO PAULO; FERNANDA C. RODRIGUES; OTAVIO BRAVIM, UnB; MAURICIO MACHAIM FRANCO, CENARGEN; MARGOT ALVES NUNES DODE, CENARGEN. |
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Título: |
The methylation patterns of the IGF2 and IGF2R genes in bovine spermatozoa are not affected by flow-cytometric sex sorting. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Molecular Reproduction e Development, v. 79, p. 77-84, 2012. |
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Idioma: |
Inglês |
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Conteúdo: |
The objectives of this study were to investigate the effect of sexing by flow cytometry on the methylation patterns of the IGF2 and IGF2R genes. Frozen-thawed, unsorted, and sex-sorted spermsamples fromfour Nellore bulls were used. Each ejaculate was separated into three fractions: non-sexed (NS), sexed forX-sperm(SX), and sexed for Y-sperm (SY). Sperm were isolated from the extender, cryoprotectant, and other cell types by centrifugation on a 40:70%Percoll gradient, and spermpellets were used for genomic DNA isolation. DNA was used for analyses of the methylation patterns by bisul?te sequencing.Methylation status of the IGF2 and IGF2Rgeneswere evaluated by sequencing 195 and 147 individual clones, respectively. No global differences in DNAmethylation were found between NS, SX, and SY groups for the IGF2 (P¼0.09) or IGF2R genes (P¼0.38). Very speci?c methylation patterns were observed in the 25th and 26th CpG sites in the IGF2R gene. representing higher methylation in NS than in the SX and SY groups compared with the other CpG sites. Further, individual variation in methylation patterns was found among bulls. In conclusion, the sex-sorting procedure by flow cytometry did not affect the overall DNA methylation patterns of the IGF2 and IGF2R genes, although individual variation in their methyla-tion patterns among bulls was observed. |
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Palavras-Chave: |
Gene IGF2; Gene IGF2R; Sexagem por citometria. |
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Thesagro: |
Bovino. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/179337/1/Carvalho-et-al-2012-Molecular-Reproduction-and-Development.pdf
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Marc: |
LEADER 02074nam a2200229 a 4500
001 1950725
005 2023-03-07
008 2012 bl uuuu u00u1 u #d
100 1 $aCARVALHO, J. O.
245 $aThe methylation patterns of the IGF2 and IGF2R genes in bovine spermatozoa are not affected by flow-cytometric sex sorting.$h[electronic resource]
260 $aMolecular Reproduction e Development, v. 79, p. 77-84$c2012
520 $aThe objectives of this study were to investigate the effect of sexing by flow cytometry on the methylation patterns of the IGF2 and IGF2R genes. Frozen-thawed, unsorted, and sex-sorted spermsamples fromfour Nellore bulls were used. Each ejaculate was separated into three fractions: non-sexed (NS), sexed forX-sperm(SX), and sexed for Y-sperm (SY). Sperm were isolated from the extender, cryoprotectant, and other cell types by centrifugation on a 40:70%Percoll gradient, and spermpellets were used for genomic DNA isolation. DNA was used for analyses of the methylation patterns by bisul?te sequencing.Methylation status of the IGF2 and IGF2Rgeneswere evaluated by sequencing 195 and 147 individual clones, respectively. No global differences in DNAmethylation were found between NS, SX, and SY groups for the IGF2 (P¼0.09) or IGF2R genes (P¼0.38). Very speci?c methylation patterns were observed in the 25th and 26th CpG sites in the IGF2R gene. representing higher methylation in NS than in the SX and SY groups compared with the other CpG sites. Further, individual variation in methylation patterns was found among bulls. In conclusion, the sex-sorting procedure by flow cytometry did not affect the overall DNA methylation patterns of the IGF2 and IGF2R genes, although individual variation in their methyla-tion patterns among bulls was observed.
650 $aBovino
653 $aGene IGF2
653 $aGene IGF2R
653 $aSexagem por citometria
700 1 $aMICHALCZECHEN-LACERDA, V. A.
700 1 $aSARTORI, R.
700 1 $aRODRIGUES, F. C.
700 1 $aBRAVIM, O.
700 1 $aFRANCO, M. M.
700 1 $aDODE, M. A. N.
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Embrapa Recursos Genéticos e Biotecnologia (CENARGEN) |
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