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| 166. |  | NOVELLI, V. M.; FREITAS-ASTÚA, J.; KITAJIMA, E. W.; GUIDOTTI, D. F. S.; MACHADO, M. A. Transmissão do vírus da leprose dos citros (CiLV-C) por populações apossimbióticas de B. phoenicis. Tropical Plant Pathology, Brasilia, DF, v. 33, supl., ago. 2008.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 167. | | KUBO, K. S.; ARENA, G. D.; KITAJIMA, E. W; MACHADO, M. A.; FREITAS-ASTUA, J. de. Transmission of citrus leprosis virus c (CiLV-C) to Arabidopsis thaliana by Brevipalpus phoenicis mites. In: CONFERENCE INTERNATIONAL ORGANIZATION CITRUS VIROLOGISTS, 18., Campinas, SP, 2010. Proceedings... Campinas: IOCV, 2010. 1 CD-ROM. 085 PS1
Publicado também em: Citrus Research & Technology, Cordeirópolis, v. 31, Suplemento, 2010| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 168. | | BOARI, A. J.; CARVALHO, T. P.; BONFIM, K.; KITAJIMA, E. W.; HAYASHI, E. E. Tomato chlorotic spot virus infecting Spilanthes oleracea in the state of Pará. In: CONGRESSO BRASILEIRO DE FITOPATOLOGIA, 46.; REUNIÃO BRASILEIRA DE CONTROLE BIOLÓGICO, 11., 2013, Ouro Preto. CBfito sustentável. Ouro Preto: UFV, 2013. 1 CD-ROM.| Biblioteca(s): Embrapa Amazônia Oriental. |
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| 169. | | RIBEIRO, S. G.; MELLO, L. V.; BOITEUX, L. S.; KITAJIMA, E. W.; FARIA, J. C. Tomato infection by a geminivirus in the Federal District, Brazil. Fitopatologia Brasileira, Brasilia, v.19, p.330, ago. 1994. Resumo. Suplemento.| Biblioteca(s): Embrapa Hortaliças. |
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| 173. | | RABELO, L. C.; PEDRAZZOLI, D. S.; NOVAES, Q. S.; NAGATA, T.; REZENDE, J. A. M.; KITAJIMA, E. W. Alta incidência de Tomato chlorotic spot virus em jiló no Estado de São Paulo. Fitopatologia Brasileira, Brasília, DF, v. 27, n. 1, p. 105, jan./fev. 2002.| Biblioteca(s): Embrapa Hortaliças. |
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| 174. | | KITAJIMA, E. W.; OLIVEIRA, F. C.; PINHEIRO, C. R. S.; SOARES, L. M.; PINHEIRO, K.; MADEIRA, M. C.; CHAGAS, M. Amarelo letal do mamoeiro solo no Estado do Rio Grande do Norte. Fitopatologia Brasileira, v.17, n.3, p.282-285, set. 1992. Artigo.| Biblioteca(s): Embrapa Tabuleiros Costeiros. |
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| 175. | | GARITA, L.; TASSI, A. D.; ASTUA, J. de F.; LOPES, J. R. S.; ALBERTI, G.; KITAJIMA, E. W. Mite vector/vírus relationships in the citrus leprosis pathosystem and an evaluation of experimental host range. In: Conference of the International Organization of Citrus Virologists 19. Mpumalanga, South Africa, 2013. Program & Abstracts... Mpumalanga, South, 2013.| Biblioteca(s): Embrapa Mandioca e Fruticultura. |
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| 176. | | COSTA, C. L.; LIN, M. T.; KITAJIMA, E. W.; SANTOS, A. A.; MESQUITA, R. C. M.; FREIRE FILHO, F. R. Cerotoma arcuata (Oliv.), um crisomelideo vector do mosaico da Vogna no Brasil. Fitopatologia Brasileira, v. 3, n. 1, p. 81-82, 1978.| Biblioteca(s): Embrapa Meio-Norte. |
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| 177. | | AGUIAR, C. L.; BAPTISTA, A. S.; WALDER, J. M. M.; TSAI, S. M.; CARRÃO-PANIZZI, M. C.; KITAJIMA, E. W. Changes in isoflavone profiles of soybean treated with gamma irradiation. International Journal of Food Sciences and Nutrition, Oxfordshire, v. 60, n. 5, p. 387-394, Aug. 2009.| Biblioteca(s): Embrapa Soja. |
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| 178. | | OLIVEIRA, A. S. de; MELO, F. L.; INOUE NAGATA, A. K.; NAGATA, T.; KITAJIMA, E. W.; RESENDE, R. O. Characterization of bean necrotic mosaic virus: a member of a novel evolutionary lineage within the genus Tospovirus. Plos One, San Francisco, v. 7, n. 6, p. 1-9, June 2012.| Biblioteca(s): Embrapa Hortaliças. |
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| 179. | | AVILA, A. C. de; HAAN, P. de; KITAJIMA, E. W.; RESENDE, R. de O.; GOLDBACH, R. W.; PETERS, D. Characterization of a distinct isolate of tomato spotted wilt virus (TSWV) from impatiens in the Netherlands. Fitopatologia Brasileira, Brasilia, v.16, n.2, p.25, jun. 1991. Resumo.| Biblioteca(s): Embrapa Hortaliças. |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
25/08/2020 |
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Data da última atualização: |
25/08/2020 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
LEASTRO, M. O.; CASTRO, D. Y. O.; ASTUA, J. de F.; KITAJIMA, E. W.; PALLÁS, V.; SÁNCHEZ-NAVARRO, J. Á. |
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Afiliação: |
MIKHAIL OLIVEIRA LEASTRO, Instituto Biológico; DEIBIS YORLENIS ORTEGA CASTRO, Universidad Politécnica de Valencia; JULIANA DE FREITAS ASTUA, CNPMF; ELLIOT WATANABE KITAJIMA, ESALQ; VICENTE PALLÁS, Universidad Politécnica de Valencia; JESÚS ÁNGEL SÁNCHEZ-NAVARRO, Universidad Politécnica de Valencia. |
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Título: |
Citrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity. |
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Ano de publicação: |
2020 |
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Fonte/Imprenta: |
Frontiers in Microbiology, June 2020. |
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ISSN: |
1664-302X |
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DOI: |
https://doi.org/10.3389/fmicb.2020.01231 |
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Idioma: |
Inglês |
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Conteúdo: |
Citrus leprosis virus C (CiLV-C) belongs to the genus Cilevirus, family Kitaviridae, and is considered the most devastating virus infecting citrus in Brazil, being the main viral pathogen responsible for citrus leprosis (CL), a severe disease that affects citrus orchards in Latin America. Here, proteins encoded by CiLV-C genomic RNA 1 and 2 were screened for potential RNA silencing suppressor (RSS) activity by five methods. Using the GFP-based reporter agroinfiltration assay, we have not found potential local suppressor activity for the five CiLV-C encoded proteins. However, when RSS activity was evaluated using the alfalfa mosaic virus (AMV) system, we found that the p29, p15, and p61 CiLV-C proteins triggered necrosis response and increased the AMV RNA 3 accumulation, suggesting a suppressive functionality. From the analysis of small interfering RNAs (siRNAs) accumulation, we observed that the ectopic expression of the p29, p15, and p61 reduced significantly the accumulation of GFP derived siRNAs. The use of the RSS defective turnip crinkle virus (TCV) system revealed that only the trans-expression of the p15 protein restored the cell-to-cell viral movement. Finally, the potato virus X (PVX) system revealed that the expression of p29, p15, and p61 increased the PVX RNA accumulation; in addition, the p29 and p15 enhanced the pathogenicity of PVX resulting in the death of tobacco plants. Furthermore, PVX-p61 infection resulted in a hypersensitive response (HR), suggesting that p61 could also activate a plant defense response mechanism. This is the first report describing the RSS activity for CiLV-C proteins and, moreover, for a member of the family Kitaviridae. MenosCitrus leprosis virus C (CiLV-C) belongs to the genus Cilevirus, family Kitaviridae, and is considered the most devastating virus infecting citrus in Brazil, being the main viral pathogen responsible for citrus leprosis (CL), a severe disease that affects citrus orchards in Latin America. Here, proteins encoded by CiLV-C genomic RNA 1 and 2 were screened for potential RNA silencing suppressor (RSS) activity by five methods. Using the GFP-based reporter agroinfiltration assay, we have not found potential local suppressor activity for the five CiLV-C encoded proteins. However, when RSS activity was evaluated using the alfalfa mosaic virus (AMV) system, we found that the p29, p15, and p61 CiLV-C proteins triggered necrosis response and increased the AMV RNA 3 accumulation, suggesting a suppressive functionality. From the analysis of small interfering RNAs (siRNAs) accumulation, we observed that the ectopic expression of the p29, p15, and p61 reduced significantly the accumulation of GFP derived siRNAs. The use of the RSS defective turnip crinkle virus (TCV) system revealed that only the trans-expression of the p15 protein restored the cell-to-cell viral movement. Finally, the potato virus X (PVX) system revealed that the expression of p29, p15, and p61 increased the PVX RNA accumulation; in addition, the p29 and p15 enhanced the pathogenicity of PVX resulting in the death of tobacco plants. Furthermore, PVX-p61 infection resulted in a hypersensitive response (HR), suggesting th... Mostrar Tudo |
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Doença de Planta; Fruta Cítrica. |
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024 7 $ahttps://doi.org/10.3389/fmicb.2020.01231$2DOI
100 1 $aLEASTRO, M. O.
245 $aCitrus Leprosis Virus C Encodes Three Proteins With Gene Silencing Suppression Activity.$h[electronic resource]
260 $c2020
520 $aCitrus leprosis virus C (CiLV-C) belongs to the genus Cilevirus, family Kitaviridae, and is considered the most devastating virus infecting citrus in Brazil, being the main viral pathogen responsible for citrus leprosis (CL), a severe disease that affects citrus orchards in Latin America. Here, proteins encoded by CiLV-C genomic RNA 1 and 2 were screened for potential RNA silencing suppressor (RSS) activity by five methods. Using the GFP-based reporter agroinfiltration assay, we have not found potential local suppressor activity for the five CiLV-C encoded proteins. However, when RSS activity was evaluated using the alfalfa mosaic virus (AMV) system, we found that the p29, p15, and p61 CiLV-C proteins triggered necrosis response and increased the AMV RNA 3 accumulation, suggesting a suppressive functionality. From the analysis of small interfering RNAs (siRNAs) accumulation, we observed that the ectopic expression of the p29, p15, and p61 reduced significantly the accumulation of GFP derived siRNAs. The use of the RSS defective turnip crinkle virus (TCV) system revealed that only the trans-expression of the p15 protein restored the cell-to-cell viral movement. Finally, the potato virus X (PVX) system revealed that the expression of p29, p15, and p61 increased the PVX RNA accumulation; in addition, the p29 and p15 enhanced the pathogenicity of PVX resulting in the death of tobacco plants. Furthermore, PVX-p61 infection resulted in a hypersensitive response (HR), suggesting that p61 could also activate a plant defense response mechanism. This is the first report describing the RSS activity for CiLV-C proteins and, moreover, for a member of the family Kitaviridae.
650 $aDoença de Planta
650 $aFruta Cítrica
700 1 $aCASTRO, D. Y. O.
700 1 $aASTUA, J. de F.
700 1 $aKITAJIMA, E. W.
700 1 $aPALLÁS, V.
700 1 $aSÁNCHEZ-NAVARRO, J. Á.
773 $tFrontiers in Microbiology, June 2020.
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