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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
12/01/2021 |
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Data da última atualização: |
12/01/2021 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
NASCIMENTO, T. R. de; SENA, P. T. S.; OLIVEIRA, G. S.; SILVA, T. R. da; DIAS, M. A. M.; FREITAS, A. D. S. de; MARTINS, L. M. V.; FERNANDES JUNIOR, P. I. |
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Afiliação: |
Tailane Ribeiro do Nascimento; Pâmella Thalita Souza Sena; Gilmar Silva Oliveira; Thaise Rosa da Silva; Marcos André Moura Dias; Ana Dolores Santiago de Freitas; Lindete Míria Vieira Martins; PAULO IVAN FERNANDES JUNIOR, CPATSA. |
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Título: |
Co-inoculation of two symbiotically efcient Bradyrhizobium strains improves cowpea development better than a single bacterium application. |
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Ano de publicação: |
2021 |
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Fonte/Imprenta: |
Biotech, v. 11, n. 1, jan. 2021. |
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DOI: |
https://doi.org/10.1007/s13205-020-02534-5 |
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Idioma: |
Inglês |
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Notas: |
Article: 4. |
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Conteúdo: |
The co-inoculation of Bradyrhizobium with other non-bradyrhizobial strains was already assessed on cowpea, but the co-inoculation of two Bradyrhizobium strains was not tested up to now. This study aimed to evaluate the cowpea growth, N accumulation, and Bradyrhizobium competitiveness of the elite strain B. pachyrhizi BR 3262 when co-inoculated with other efcient Bradyrhizobium from the Brazilian semiarid region. Three potted-plant experiments were carried out. In the frst assay, 35 efcient Bradyrhizobium isolates obtained from the semiarid region of Brazil were co-inoculated with the elite strains B. pachyrhizi BR 3262. The experiment was conducted in gnotobiotic conditions. The plant growth, nodulation, N nutritional variables, and nodular occupation were assessed. Under gnotobiotic and non-sterile soil conditions, ten selected bacteria plus the elite strain B. yuanmingense BR 3267 were used at the second and third experiments, respectively. The cowpea was inoculated with the 11 bacteria individually or co-inoculated with BR 3262. The plant growth and N nutritional variables were assessed. A double-layer medium spot method experiment was conducted to evaluate the interaction among the co-inoculated strains in standard and diluted YMA media. The co-inoculation treatments showed the best efciency when compared to the treatments inoculated solely with BR 3262. This strain occupied a low amount of cowpea nodules ranging from 5 to 67.5%. The treatments with lower BR 3262 nodule occupancy showed the best results for the shoot nitrogen accumulation. The culture experiment showed that four bacteria inhibited the growth of BR 3262. In contrast, seven strains from the soils of Brazilian semiarid region were benefted by the previous inoculation of this strain. In the second and third experiments, the results indicated that all 11 co-inoculated treatments were more efcient than the single inoculation, proofng the best performance of the dual inoculation of Bradyrhizobium on cowpea. MenosThe co-inoculation of Bradyrhizobium with other non-bradyrhizobial strains was already assessed on cowpea, but the co-inoculation of two Bradyrhizobium strains was not tested up to now. This study aimed to evaluate the cowpea growth, N accumulation, and Bradyrhizobium competitiveness of the elite strain B. pachyrhizi BR 3262 when co-inoculated with other efcient Bradyrhizobium from the Brazilian semiarid region. Three potted-plant experiments were carried out. In the frst assay, 35 efcient Bradyrhizobium isolates obtained from the semiarid region of Brazil were co-inoculated with the elite strains B. pachyrhizi BR 3262. The experiment was conducted in gnotobiotic conditions. The plant growth, nodulation, N nutritional variables, and nodular occupation were assessed. Under gnotobiotic and non-sterile soil conditions, ten selected bacteria plus the elite strain B. yuanmingense BR 3267 were used at the second and third experiments, respectively. The cowpea was inoculated with the 11 bacteria individually or co-inoculated with BR 3262. The plant growth and N nutritional variables were assessed. A double-layer medium spot method experiment was conducted to evaluate the interaction among the co-inoculated strains in standard and diluted YMA media. The co-inoculation treatments showed the best efciency when compared to the treatments inoculated solely with BR 3262. This strain occupied a low amount of cowpea nodules ranging from 5 to 67.5%. The treatments with lower BR 3262 nodule ... Mostrar Tudo |
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Palavras-Chave: |
Feijão caupi; Fxação biológica de nitrogênio; Inoculação de Dual; Seleção de cepas. |
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Thesagro: |
Feijão; Inoculante; Vigna Unguiculata. |
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Thesaurus Nal: |
Bradyrhizobium; Cowpeas. |
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Categoria do assunto: |
F Plantas e Produtos de Origem Vegetal |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/220209/1/Co8209inoculation-of-two-symbiotically-efcient-Bradyrhizobium-2020.pdf
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Marc: |
LEADER 03051naa a2200337 a 4500
001 2129244
005 2021-01-12
008 2021 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s13205-020-02534-5$2DOI
100 1 $aNASCIMENTO, T. R. de
245 $aCo-inoculation of two symbiotically efcient Bradyrhizobium strains improves cowpea development better than a single bacterium application.$h[electronic resource]
260 $c2021
500 $aArticle: 4.
520 $aThe co-inoculation of Bradyrhizobium with other non-bradyrhizobial strains was already assessed on cowpea, but the co-inoculation of two Bradyrhizobium strains was not tested up to now. This study aimed to evaluate the cowpea growth, N accumulation, and Bradyrhizobium competitiveness of the elite strain B. pachyrhizi BR 3262 when co-inoculated with other efcient Bradyrhizobium from the Brazilian semiarid region. Three potted-plant experiments were carried out. In the frst assay, 35 efcient Bradyrhizobium isolates obtained from the semiarid region of Brazil were co-inoculated with the elite strains B. pachyrhizi BR 3262. The experiment was conducted in gnotobiotic conditions. The plant growth, nodulation, N nutritional variables, and nodular occupation were assessed. Under gnotobiotic and non-sterile soil conditions, ten selected bacteria plus the elite strain B. yuanmingense BR 3267 were used at the second and third experiments, respectively. The cowpea was inoculated with the 11 bacteria individually or co-inoculated with BR 3262. The plant growth and N nutritional variables were assessed. A double-layer medium spot method experiment was conducted to evaluate the interaction among the co-inoculated strains in standard and diluted YMA media. The co-inoculation treatments showed the best efciency when compared to the treatments inoculated solely with BR 3262. This strain occupied a low amount of cowpea nodules ranging from 5 to 67.5%. The treatments with lower BR 3262 nodule occupancy showed the best results for the shoot nitrogen accumulation. The culture experiment showed that four bacteria inhibited the growth of BR 3262. In contrast, seven strains from the soils of Brazilian semiarid region were benefted by the previous inoculation of this strain. In the second and third experiments, the results indicated that all 11 co-inoculated treatments were more efcient than the single inoculation, proofng the best performance of the dual inoculation of Bradyrhizobium on cowpea.
650 $aBradyrhizobium
650 $aCowpeas
650 $aFeijão
650 $aInoculante
650 $aVigna Unguiculata
653 $aFeijão caupi
653 $aFxação biológica de nitrogênio
653 $aInoculação de Dual
653 $aSeleção de cepas
700 1 $aSENA, P. T. S.
700 1 $aOLIVEIRA, G. S.
700 1 $aSILVA, T. R. da
700 1 $aDIAS, M. A. M.
700 1 $aFREITAS, A. D. S. de
700 1 $aMARTINS, L. M. V.
700 1 $aFERNANDES JUNIOR, P. I.
773 $tBiotech$gv. 11, n. 1, jan. 2021.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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Registro Completo
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Biblioteca(s): |
Embrapa Gado de Corte. |
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Data corrente: |
01/07/2003 |
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Data da última atualização: |
04/07/2023 |
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Autoria: |
MADRUGA, C. R.; LEAL, C. R. B.; FERREIRA, A. M. T.; ARAUJO, F. R.; BONATO, A. L. V.; KESSLER, R. H.; SCHENK, M. A. M.; SOARES, C. O. |
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Afiliação: |
CLAUDIO ROBERTO MADRUGA, CNPGC; CÁSSIA R. B. LEAL, UNIVERSIDADE CATÓLICA DOM BOSCO; ALDA M. T. FERREIRA, UNIVERSIDADE CATÓLICA DOM BOSCO; FLABIO RIBEIRO DE ARAUJO, CNPGC; ANA LIDIA VARIANI BONATO, CNPGC; RAUL HENRIQUE KESSLER, CNPGC; MARIA APARECIDA MOREIRA SCHENK, CNPGC; CLEBER OLIVEIRA SOARES, CNPGC. |
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Título: |
Genetic and antigenic analysis of Babesia bigemina isolates from five geographical regions of Brazil. |
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Ano de publicação: |
2002 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, v. 22, n. 4, p. 153-160, out./dez. 2002. |
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DOI: |
https://doi.org/10.1590/S0100-736X2002000400005 |
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Idioma: |
Inglês |
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Conteúdo: |
A molecular epidemiological study was performed with Babesia bigemina isolates from five geographical regions of Brazil. The genetic analysis was done with random amplification of polymorphic DNA (RAPD), repetitive extragenic palindromic elements-polymerase chain reaction (REP-PCR) and enterobacterial repetitive intergenic consensus sequences-polymerase chain reaction (ERIC-PCR) that showed genetic polymorphism between these isolates and generated fingerprinting. In RAPD, ILO872 and ILO876 primers were able to detect at least one fingerprinting for each B. bigemina isolate. The amplification of B. bigemina DNA fragments by REP-PCR and ERIC-PCR gave evidence for the presence in this haemoprotozoan of the sequences described previously in microorganisms of the bacterial kingdom. For the first time it was demonstrated that both techniques can be used for genetic analysis of a protozoan parasite, although the ERIC-PCR was more discriminatory than REP-PCR. The dendogram with similarity coefficient among isolates showed two clusters and one subcluster. The Northeastern and Mid-Western isolates showed the greatest genetic diversity, while the Southeastern and Southern isolates were the closest. The antigenic analysis was done through indirect fluorescent antibody technique and Western blotting using a panel of monoclonal antibodies directed against epitopes on the merozoite membrane surface, rhoptries and membrane of infected erythrocytes. As expected, the merozoite variable surface antigens, major surface antigen (MSA)-1 and MSA-2 showed antigenic diversity. However, B cell epitopes on rhoptries and infected erythrocytes were conserved among all isolates studied. In this study it was possible to identify variable and conserved antigens, which had already been described as potential immunogens. Considering that an attenuated Babesia clone used as immunogen selected populations capable of evading the immunity induced by this vaccine, it is necessary to evaluate more deeply the cross-protection conferred by genetically more distant Brazilian B. bigemina isolates and make an evaluation of the polymorphism degree of variable antigens such as MSA-1 and MSA-2. MenosA molecular epidemiological study was performed with Babesia bigemina isolates from five geographical regions of Brazil. The genetic analysis was done with random amplification of polymorphic DNA (RAPD), repetitive extragenic palindromic elements-polymerase chain reaction (REP-PCR) and enterobacterial repetitive intergenic consensus sequences-polymerase chain reaction (ERIC-PCR) that showed genetic polymorphism between these isolates and generated fingerprinting. In RAPD, ILO872 and ILO876 primers were able to detect at least one fingerprinting for each B. bigemina isolate. The amplification of B. bigemina DNA fragments by REP-PCR and ERIC-PCR gave evidence for the presence in this haemoprotozoan of the sequences described previously in microorganisms of the bacterial kingdom. For the first time it was demonstrated that both techniques can be used for genetic analysis of a protozoan parasite, although the ERIC-PCR was more discriminatory than REP-PCR. The dendogram with similarity coefficient among isolates showed two clusters and one subcluster. The Northeastern and Mid-Western isolates showed the greatest genetic diversity, while the Southeastern and Southern isolates were the closest. The antigenic analysis was done through indirect fluorescent antibody technique and Western blotting using a panel of monoclonal antibodies directed against epitopes on the merozoite membrane surface, rhoptries and membrane of infected erythrocytes. As expected, the merozoite variable surfac... Mostrar Tudo |
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Thesagro: |
Antígeno; Babesia Bigemina; Polimorfismo Genético. |
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Thesaurus NAL: |
Antigens; Genetic polymorphism. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/253106/1/Genetic-antigenic-analysis-2002.pdf
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Marc: |
LEADER 03037naa a2200277 a 4500
001 1325288
005 2023-07-04
008 2002 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1590/S0100-736X2002000400005$2DOI
100 1 $aMADRUGA, C. R.
245 $aGenetic and antigenic analysis of Babesia bigemina isolates from five geographical regions of Brazil.
260 $c2002
520 $aA molecular epidemiological study was performed with Babesia bigemina isolates from five geographical regions of Brazil. The genetic analysis was done with random amplification of polymorphic DNA (RAPD), repetitive extragenic palindromic elements-polymerase chain reaction (REP-PCR) and enterobacterial repetitive intergenic consensus sequences-polymerase chain reaction (ERIC-PCR) that showed genetic polymorphism between these isolates and generated fingerprinting. In RAPD, ILO872 and ILO876 primers were able to detect at least one fingerprinting for each B. bigemina isolate. The amplification of B. bigemina DNA fragments by REP-PCR and ERIC-PCR gave evidence for the presence in this haemoprotozoan of the sequences described previously in microorganisms of the bacterial kingdom. For the first time it was demonstrated that both techniques can be used for genetic analysis of a protozoan parasite, although the ERIC-PCR was more discriminatory than REP-PCR. The dendogram with similarity coefficient among isolates showed two clusters and one subcluster. The Northeastern and Mid-Western isolates showed the greatest genetic diversity, while the Southeastern and Southern isolates were the closest. The antigenic analysis was done through indirect fluorescent antibody technique and Western blotting using a panel of monoclonal antibodies directed against epitopes on the merozoite membrane surface, rhoptries and membrane of infected erythrocytes. As expected, the merozoite variable surface antigens, major surface antigen (MSA)-1 and MSA-2 showed antigenic diversity. However, B cell epitopes on rhoptries and infected erythrocytes were conserved among all isolates studied. In this study it was possible to identify variable and conserved antigens, which had already been described as potential immunogens. Considering that an attenuated Babesia clone used as immunogen selected populations capable of evading the immunity induced by this vaccine, it is necessary to evaluate more deeply the cross-protection conferred by genetically more distant Brazilian B. bigemina isolates and make an evaluation of the polymorphism degree of variable antigens such as MSA-1 and MSA-2.
650 $aAntigens
650 $aGenetic polymorphism
650 $aAntígeno
650 $aBabesia Bigemina
650 $aPolimorfismo Genético
700 1 $aLEAL, C. R. B.
700 1 $aFERREIRA, A. M. T.
700 1 $aARAUJO, F. R.
700 1 $aBONATO, A. L. V.
700 1 $aKESSLER, R. H.
700 1 $aSCHENK, M. A. M.
700 1 $aSOARES, C. O.
773 $tPesquisa Veterinária Brasileira$gv. 22, n. 4, p. 153-160, out./dez. 2002.
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Embrapa Gado de Corte (CNPGC) |
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