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1. | | COUTINHO, H. L. da C.; KAY, H. E.; MANFIO, G. P.; NEVES, M. C. P.; RIBEIRO, J. R. A.; RUMJANEK, N. G.; BERINGER, J. E. Molecular evidence for shifts in polysaccharide composition associated with adaptation of soybean Bradyrhizobium strains to the Brazilian Cerrado soils Environmental Microbiology, v. 1, n. 5, p. 401-408, Sept. 1999. Biblioteca(s): Embrapa Solos. |
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Registros recuperados : 1 | |
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| Acesso ao texto completo restrito à biblioteca da Embrapa Solos. Para informações adicionais entre em contato com cnps.biblioteca@embrapa.br. |
Registro Completo
Biblioteca(s): |
Embrapa Solos. |
Data corrente: |
22/11/2002 |
Data da última atualização: |
20/09/2021 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
Internacional - A |
Autoria: |
COUTINHO, H. L. da C.; KAY, H. E.; MANFIO, G. P.; NEVES, M. C. P.; RIBEIRO, J. R. A.; RUMJANEK, N. G.; BERINGER, J. E. |
Afiliação: |
HEITOR LUIZ DA COSTA COUTINHO, CNPS; HAZEL E. KAY, UNIVERSITY OF BRISTOL; GILSON PAULO MANFIO, UNIVERSITY OF NEWCASTLE; MARIA CRISTINA PRATA NEVES, CNPAB; JOSÉ ROBERTO A. RIBEIRO, CNPAB; NORMA GOUVEA RUMJANEK, CNPAB; JOHN E. BERINGER, UNIVERSITY OF BRISTOL. |
Título: |
Molecular evidence for shifts in polysaccharide composition associated with adaptation of soybean Bradyrhizobium strains to the Brazilian Cerrado soils |
Ano de publicação: |
1999 |
Fonte/Imprenta: |
Environmental Microbiology, v. 1, n. 5, p. 401-408, Sept. 1999. |
DOI: |
https://doi.org/10.1046/j.1462-2920.1999.00047.x |
Idioma: |
Inglês |
Conteúdo: |
Pyrolysis mass spectrometry (PyMS) and DNA fingerprinting (RAPD and RSa hybridization) were used to characterize soybean inoculant strains and root nodule isolates of bradyrhizobia from the Brazilian Cerrado soils. Most isolates were shown to be derived from the inoculant strains on the basis of genotype comparisons by DNA fingerprinting. Phenotypic analysis (using PyMS) of the strains and separately of the polysaccharides derived from them showed that the nodule isolates differed from the parental strains, suggesting adaptation to the Cerrado soil environment. The extent of the differences between the derivatives and inoculant strains was similar for comparisons made on the basis of whole-cell preparations or from the isolated polysaccharides, indicating that the adaptation was caused by changes in the composition of the polysaccharides produced. |
Thesagro: |
Cerrado; Soja; Solo. |
Thesaurus NAL: |
DNA fingerprinting; Mass spectrometry; Pyrolysis. |
Categoria do assunto: |
P Recursos Naturais, Ciências Ambientais e da Terra |
Marc: |
LEADER 01746naa a2200277 a 4500 001 1336379 005 2021-09-20 008 1999 bl uuuu u00u1 u #d 024 7 $ahttps://doi.org/10.1046/j.1462-2920.1999.00047.x$2DOI 100 1 $aCOUTINHO, H. L. da C. 245 $aMolecular evidence for shifts in polysaccharide composition associated with adaptation of soybean Bradyrhizobium strains to the Brazilian Cerrado soils$h[electronic resource] 260 $c1999 520 $aPyrolysis mass spectrometry (PyMS) and DNA fingerprinting (RAPD and RSa hybridization) were used to characterize soybean inoculant strains and root nodule isolates of bradyrhizobia from the Brazilian Cerrado soils. Most isolates were shown to be derived from the inoculant strains on the basis of genotype comparisons by DNA fingerprinting. Phenotypic analysis (using PyMS) of the strains and separately of the polysaccharides derived from them showed that the nodule isolates differed from the parental strains, suggesting adaptation to the Cerrado soil environment. The extent of the differences between the derivatives and inoculant strains was similar for comparisons made on the basis of whole-cell preparations or from the isolated polysaccharides, indicating that the adaptation was caused by changes in the composition of the polysaccharides produced. 650 $aDNA fingerprinting 650 $aMass spectrometry 650 $aPyrolysis 650 $aCerrado 650 $aSoja 650 $aSolo 700 1 $aKAY, H. E. 700 1 $aMANFIO, G. P. 700 1 $aNEVES, M. C. P. 700 1 $aRIBEIRO, J. R. A. 700 1 $aRUMJANEK, N. G. 700 1 $aBERINGER, J. E. 773 $tEnvironmental Microbiology$gv. 1, n. 5, p. 401-408, Sept. 1999.
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