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Registro Completo |
Biblioteca(s): |
Embrapa Agroenergia; Embrapa Milho e Sorgo. |
Data corrente: |
19/09/2017 |
Data da última atualização: |
15/02/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
SOUSA, C. A. F. de; PAIVA, D S. de; CASARI, R. A. das C. N.; MOLINARI, H. B. C.; OLIVEIRA, N. G. de; KOBAYASHI, A. K.; MAGALHAES, P. C.; GOMIDE, R. L.; SOUZA JUNIOR, M. T. |
Afiliação: |
CARLOS ANTONIO FERREIRA DE SOUSA, CNPAE; DAYANE SILVA DE PAIVA; RAPHAEL AUGUSTO DAS CHAGAS NOQUELI CASARI; HUGO BRUNO CORREA MOLINARI, CNPAE; NELSON GERALDO DE OLIVEIRA; ADILSON KENJI KOBAYASHI, CNPAE; PAULO CESAR MAGALHAES, CNPMS; REINALDO LUCIO GOMIDE, CNPMS; MANOEL TEIXEIRA SOUZA JUNIOR, CNPAE. |
Título: |
A procedure for maize genotypes discrimination to drought by chlorophyll fluorescence imaging rapid light curves. |
Ano de publicação: |
2017 |
Fonte/Imprenta: |
Plant Methods, v. 13, p. 1-17, 2017. |
DOI: |
10.1186/s13007-017-0209-z |
Idioma: |
Inglês |
Conteúdo: |
Background: Photosynthesis can be roughly separated into biochemical and photochemical processes. Both are affected by drought and can be assessed by non-invasive standard methods. Gas exchange, which mainly assesses the first process, has well-defined protocols. It is considered a standard method for evaluation of plant responses to drought. Under such stress, assessment of photochemical apparatus by chlorophyll fluorescence needs improvement to become faster and reproducible, especially in growing plants under field conditions. For this, we developed a protocol based on chlorophyll fluorescence imaging, using a rapid light curve approach. Results: Almost all parameters obtained by rapid light curves have shown statistical differences between control and drought stressed maize plants. However, most of them were affected by induction processes, relaxation rate, and/or differences in chlorophyll content; while they all were influenced by actinic light intensity on each light step of light curve. Only the normalized parameters related to photochemical and non-photochemical quenching were strongly correlated with data obtained by gas exchange, but only from the light step in which the linear electron flow reached saturation. Conclusions: The procedure developed in this study for discrimination of plant responses to water deficit stress proved to be as fast, efficient and reliable as the standard technique of gas exchange in order to discriminate the responses of maize genotypes to drought. However, unlike that, there is no need to perform daily and time consuming calibration routines. Moreover, plant acclimation to the dark is not required. The protocol can be applied to plants growing in both controlled conditions and full sunlight in the field. In addition, it generates parameters in a fast and accurate measurement process, which enables evaluating several plants in a short period of time. MenosBackground: Photosynthesis can be roughly separated into biochemical and photochemical processes. Both are affected by drought and can be assessed by non-invasive standard methods. Gas exchange, which mainly assesses the first process, has well-defined protocols. It is considered a standard method for evaluation of plant responses to drought. Under such stress, assessment of photochemical apparatus by chlorophyll fluorescence needs improvement to become faster and reproducible, especially in growing plants under field conditions. For this, we developed a protocol based on chlorophyll fluorescence imaging, using a rapid light curve approach. Results: Almost all parameters obtained by rapid light curves have shown statistical differences between control and drought stressed maize plants. However, most of them were affected by induction processes, relaxation rate, and/or differences in chlorophyll content; while they all were influenced by actinic light intensity on each light step of light curve. Only the normalized parameters related to photochemical and non-photochemical quenching were strongly correlated with data obtained by gas exchange, but only from the light step in which the linear electron flow reached saturation. Conclusions: The procedure developed in this study for discrimination of plant responses to water deficit stress proved to be as fast, efficient and reliable as the standard technique of gas exchange in order to discriminate the responses of maize genotypes... Mostrar Tudo |
Palavras-Chave: |
Phenotyping; Water deficit; Zea mays L. |
Thesaurus Nal: |
abiotic stress; corn; gas exchange; phenomics. |
Categoria do assunto: |
-- F Plantas e Produtos de Origem Vegetal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/168295/1/Procedure-maize.pdf
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Marc: |
LEADER 02834naa a2200313 a 4500 001 2081609 005 2018-02-15 008 2017 bl uuuu u00u1 u #d 024 7 $a10.1186/s13007-017-0209-z$2DOI 100 1 $aSOUSA, C. A. F. de 245 $aA procedure for maize genotypes discrimination to drought by chlorophyll fluorescence imaging rapid light curves.$h[electronic resource] 260 $c2017 520 $aBackground: Photosynthesis can be roughly separated into biochemical and photochemical processes. Both are affected by drought and can be assessed by non-invasive standard methods. Gas exchange, which mainly assesses the first process, has well-defined protocols. It is considered a standard method for evaluation of plant responses to drought. Under such stress, assessment of photochemical apparatus by chlorophyll fluorescence needs improvement to become faster and reproducible, especially in growing plants under field conditions. For this, we developed a protocol based on chlorophyll fluorescence imaging, using a rapid light curve approach. Results: Almost all parameters obtained by rapid light curves have shown statistical differences between control and drought stressed maize plants. However, most of them were affected by induction processes, relaxation rate, and/or differences in chlorophyll content; while they all were influenced by actinic light intensity on each light step of light curve. Only the normalized parameters related to photochemical and non-photochemical quenching were strongly correlated with data obtained by gas exchange, but only from the light step in which the linear electron flow reached saturation. Conclusions: The procedure developed in this study for discrimination of plant responses to water deficit stress proved to be as fast, efficient and reliable as the standard technique of gas exchange in order to discriminate the responses of maize genotypes to drought. However, unlike that, there is no need to perform daily and time consuming calibration routines. Moreover, plant acclimation to the dark is not required. The protocol can be applied to plants growing in both controlled conditions and full sunlight in the field. In addition, it generates parameters in a fast and accurate measurement process, which enables evaluating several plants in a short period of time. 650 $aabiotic stress 650 $acorn 650 $agas exchange 650 $aphenomics 653 $aPhenotyping 653 $aWater deficit 653 $aZea mays L 700 1 $aPAIVA, D S. de 700 1 $aCASARI, R. A. das C. N. 700 1 $aMOLINARI, H. B. C. 700 1 $aOLIVEIRA, N. G. de 700 1 $aKOBAYASHI, A. K. 700 1 $aMAGALHAES, P. C. 700 1 $aGOMIDE, R. L. 700 1 $aSOUZA JUNIOR, M. T. 773 $tPlant Methods$gv. 13, p. 1-17, 2017.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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Registro Completo
Biblioteca(s): |
Embrapa Recursos Genéticos e Biotecnologia. |
Data corrente: |
23/02/2015 |
Data da última atualização: |
26/04/2024 |
Tipo da produção científica: |
Resumo em Anais de Congresso |
Autoria: |
LIMA, E. A.; CARNEIRO, F. A.; COSTA, T. S.; RÊGO, E. C. S.; JORGE JÚNIOR, A.; FURLANETTO, C.; MARRACCINI, P.; CARNEIRO, R. M. D. G.; ANDRADE, A. C. |
Afiliação: |
E. A. LIMA, UNB; F. A. CARNEIRO; TANIA DA SILVEIRA AGOSTINI COSTA, CENARGEN; E. C. S. RÊGO; A. JORGE JÚNIOR; C. FURLANETTO, UNB; P. MARRACCINI, CIRAD UMR AGAP; REGINA MARIA DECHECHI G CARNEIRO, CENARGEN; ALAN CARVALHO ANDRADE, CENARGEN. |
Título: |
Molecular characterization of resistance responses of Coffea canephora 'clone 14' upon infection with Meloidogyne paranaensis. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Journal of Nematology, v. 46, n. 2, p. 194, June 2014. |
Idioma: |
Inglês |
Notas: |
Edição dos Proceedings do 6th International Congress of Nematology, Cape Town, South Africa, May 2014. |
Palavras-Chave: |
Meloidogyne paranaensis. |
Thesagro: |
Coffea Canephora. |
Categoria do assunto: |
-- |
Marc: |
LEADER 00845nam a2200229 a 4500 001 2009387 005 2024-04-26 008 2014 bl uuuu u00u1 u #d 100 1 $aLIMA, E. A. 245 $aMolecular characterization of resistance responses of Coffea canephora 'clone 14' upon infection with Meloidogyne paranaensis.$h[electronic resource] 260 $aJournal of Nematology, v. 46, n. 2, p. 194, June 2014.$c2014 500 $aEdição dos Proceedings do 6th International Congress of Nematology, Cape Town, South Africa, May 2014. 650 $aCoffea Canephora 653 $aMeloidogyne paranaensis 700 1 $aCARNEIRO, F. A. 700 1 $aCOSTA, T. S. 700 1 $aRÊGO, E. C. S. 700 1 $aJORGE JÚNIOR, A. 700 1 $aFURLANETTO, C. 700 1 $aMARRACCINI, P. 700 1 $aCARNEIRO, R. M. D. G. 700 1 $aANDRADE, A. C.
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