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Registro Completo |
Biblioteca(s): |
Embrapa Arroz e Feijão; Embrapa Hortaliças; Embrapa Meio-Norte. |
Data corrente: |
20/05/1994 |
Data da última atualização: |
24/08/2006 |
Autoria: |
MORAES, G. J. de. |
Título: |
Aspectos entomológicos das culturas do feijão, tomate, melancia e banana. |
Ano de publicação: |
1979 |
Fonte/Imprenta: |
Petrolina: EMBRAPA-CPATSA, 1979. |
Páginas: |
28 p. |
Idioma: |
Português |
Notas: |
Trabalho apresentado no Treinamento oferecido aos técnicos do Programa de Pesquisa do Projeto Sertanejo, de 11 a 25 de setembro de 1979. |
Conteúdo: |
A importância de cada inseto ou ácaro que ataca as culturas do feijão, tomate, melancia e banana, o seu controle para que a cultura produza satisfatoriamente. |
Palavras-Chave: |
Bean; Caupi; Control; Controle; Pest insects; Watermelon. |
Thesagro: |
Banana; Citrullus Lanatus; Doença; Feijão; Inseto; Lycopersicon Esculentum; Melancia; Phaseolus Vulgaris; Praga; Tomate; Vigna Unguiculata. |
Thesaurus Nal: |
tomatoes. |
Categoria do assunto: |
-- |
Marc: |
LEADER 01129nam a2200349 a 4500 001 1195561 005 2006-08-24 008 1979 bl uuuu u0uu1 u #d 100 1 $aMORAES, G. J. de 245 $aAspectos entomológicos das culturas do feijão, tomate, melancia e banana. 260 $aPetrolina: EMBRAPA-CPATSA$c1979 300 $a28 p. 500 $aTrabalho apresentado no Treinamento oferecido aos técnicos do Programa de Pesquisa do Projeto Sertanejo, de 11 a 25 de setembro de 1979. 520 $aA importância de cada inseto ou ácaro que ataca as culturas do feijão, tomate, melancia e banana, o seu controle para que a cultura produza satisfatoriamente. 650 $atomatoes 650 $aBanana 650 $aCitrullus Lanatus 650 $aDoença 650 $aFeijão 650 $aInseto 650 $aLycopersicon Esculentum 650 $aMelancia 650 $aPhaseolus Vulgaris 650 $aPraga 650 $aTomate 650 $aVigna Unguiculata 653 $aBean 653 $aCaupi 653 $aControl 653 $aControle 653 $aPest insects 653 $aWatermelon
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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Registro Completo
Biblioteca(s): |
Embrapa Gado de Corte; Embrapa Gado de Leite. |
Data corrente: |
17/03/2014 |
Data da última atualização: |
05/02/2024 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
A - 1 |
Autoria: |
ARAUJO, C. P.; OSÓRIO, A. L. A. R.; JORGE, K. S. G.; RAMOS, C. A. N.; S. FILHO, A. F.; VIDAL, C. E. S.; ROXO, E.; NISHIBE, c.; ALMEIDA, N. F.; F. JUNIOR, A. A.; SILVA, M. R.; B. NETO, J. D.; CERQUEIRA, V. D.; ZUMÁRRAGA, M.; ARAUJO, F. R. |
Afiliação: |
MARCIO ROBERTO SILVA, CNPGL. |
Título: |
Detection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1. |
Ano de publicação: |
2014 |
Fonte/Imprenta: |
Plos One, v. 9, n. 3, p. 1-6, 2014 |
Idioma: |
Inglês Português |
Conteúdo: |
In the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with NVL. However, nested-PCR detected a significantly higher number of positive animals than the culture in the group of animals exhibiting LCT with no previous records of CITT. The use of the nested-PCR assay to detect M. bovis in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis. MenosIn the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with ... Mostrar Tudo |
Palavras-Chave: |
Bovine and bubaline tuberculosis; Nested-PCR system. |
Thesaurus NAL: |
Mycobacterium abscessus. |
Categoria do assunto: |
-- L Ciência Animal e Produtos de Origem Animal |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/119848/1/ARAUJO-C.-P..pdf
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/99300/1/pone.0091023-1..6-2014-Nested-PCR.pdf
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Marc: |
LEADER 02739naa a2200325 a 4500 001 2010358 005 2024-02-05 008 2014 bl uuuu u00u1 u #d 100 1 $aARAUJO, C. P. 245 $aDetection of Mycobacterium bovis in Bovine and Bubaline Tissues Using Nested-PCR for TbD1.$h[electronic resource] 260 $c2014 520 $aIn the present study, a nested-PCR system, targeting the TbD1 region, involving the performance of conventional PCR followed by real-time PCR, was developed to detect Mycobacterium bovis in bovine/bubaline tissue homogenates. The sensitivity and specificity of the reactions were assessed with DNA samples extracted from tuberculous and non-tuberculous mycobacteria, as well as other actinomycetales species and DNA samples extracted directly from bovine and bubaline tissue homogenates. In terms of analytical sensitivity, the DNA of M. bovis AN5 was detected up to 1.56 ng with conventional PCR, 97.6 pg with real-time PCR, and 1.53 pg with nested-PCR in the reaction mixture. The nested-PCR exhibited 100% analytical specificity for M. bovis when tested with the DNA of reference strains of environmental mycobacteria and closely-related Actinomycetales. A clinical sensitivity value of 76.0% was detected with tissue samples from animals that exhibited positive results in the comparative intradermal tuberculin test (CITT), as well as from those with lesions compatible with tuberculosis (LCT) that rendered positive cultures. A clinical specificity value of 100% was detected with tissue samples from animals with CITT- results, with no visible lesions (NVL) and negative cultures. No significant differences were found between the nested-PCR and culture in terms of detecting CITT+ animals with LCT or with NVL. No significant differences were recorded in the detection of CITT- animals with NVL. However, nested-PCR detected a significantly higher number of positive animals than the culture in the group of animals exhibiting LCT with no previous records of CITT. The use of the nested-PCR assay to detect M. bovis in tissue homogenates provided a rapid diagnosis of bovine and bubaline tuberculosis. 650 $aMycobacterium abscessus 653 $aBovine and bubaline tuberculosis 653 $aNested-PCR system 700 1 $aOSÓRIO, A. L. A. R. 700 1 $aJORGE, K. S. G. 700 1 $aRAMOS, C. A. N. 700 1 $aS. FILHO, A. F. 700 1 $aVIDAL, C. E. S. 700 1 $aROXO, E. 700 1 $aNISHIBE, c. 700 1 $aALMEIDA, N. F. 700 1 $aF. JUNIOR, A. A. 700 1 $aSILVA, M. R. 700 1 $aB. NETO, J. D. 700 1 $aCERQUEIRA, V. D. 700 1 $aZUMÁRRAGA, M. 700 1 $aARAUJO, F. R. 773 $tPlos One$gv. 9, n. 3, p. 1-6, 2014
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Embrapa Gado de Leite (CNPGL) |
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