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Registros recuperados : 226 | |
Registros recuperados : 226 | |
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Registro Completo
Biblioteca(s): |
Embrapa Meio Ambiente. |
Data corrente: |
02/12/2009 |
Data da última atualização: |
03/06/2016 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Circulação/Nível: |
B - 1 |
Autoria: |
JONSSON, C. M.; AOYAMA, H. |
Afiliação: |
CLAUDIO MARTIN JONSSON, CNPMA; HIROSHI AOYAMA, IB-UNICAMP. |
Título: |
Effect of copper on the activation of the acid phosphatase from the green algae Pseudokirchneriella subcapitata. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
Biometals, v. 23, n. 1, p. 93-98, Sep. 2010. |
DOI: |
10.1007/s10534-009-9270-z |
Idioma: |
Português |
Conteúdo: |
The in vitro activation effect of copper on the acid phosphatase of the green algae Pseudokirch- neriella subcapitata (formely Selenastrum capricor- nutum) under preincubation condition. Apparent Michaelis constant values of 1.21 and 0.37 mM, and activation energy values of 26.8 and 13.6 kJ mol -1 were determined in the absence and in the presence of 0.2 mM Cu2?, respectively. The dissociation constant value for Cu2? binding to the enzyme was determined to be 22.04 lM. The decrease of the apparent Michaelis constant (Km) and activation energy values in the presence of Cu2? correlates well with its activating effect on the acid phosphatase activity. This propriety could be used as a sensitive bioindicator for copper in environmental samples. |
Palavras-Chave: |
Ecotoxicologia. |
Categoria do assunto: |
W Química e Física |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/143926/1/2009AP-29.pdf
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Marc: |
LEADER 01259naa a2200157 a 4500 001 1577024 005 2016-06-03 008 2010 bl uuuu u00u1 u #d 024 7 $a10.1007/s10534-009-9270-z$2DOI 100 1 $aJONSSON, C. M. 245 $aEffect of copper on the activation of the acid phosphatase from the green algae Pseudokirchneriella subcapitata.$h[electronic resource] 260 $c2010 520 $aThe in vitro activation effect of copper on the acid phosphatase of the green algae Pseudokirch- neriella subcapitata (formely Selenastrum capricor- nutum) under preincubation condition. Apparent Michaelis constant values of 1.21 and 0.37 mM, and activation energy values of 26.8 and 13.6 kJ mol -1 were determined in the absence and in the presence of 0.2 mM Cu2?, respectively. The dissociation constant value for Cu2? binding to the enzyme was determined to be 22.04 lM. The decrease of the apparent Michaelis constant (Km) and activation energy values in the presence of Cu2? correlates well with its activating effect on the acid phosphatase activity. This propriety could be used as a sensitive bioindicator for copper in environmental samples. 653 $aEcotoxicologia 700 1 $aAOYAMA, H. 773 $tBiometals$gv. 23, n. 1, p. 93-98, Sep. 2010.
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Embrapa Meio Ambiente (CNPMA) |
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