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| 2. |  | SANTOS, C. S. dos; JESUS, V. L. T. de; McINTOSH, D.; BERTO, B. P.; LOPES, C. W. G. Co-infection by Tritrichomonas foetus and Pentatrichomonas hominis in asymptomatic cats. Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 12, p. 980-988, dez. 2015.| Biblioteca(s): Embrapa Unidades Centrais. |
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| 3. | | OLIVEIRA, V. M.; FONSECA, A. H.; PEREIRA, M. J. S.; CARNEIRO, A. V.; JESUS, V. L. T.; ALVES, P. A. M. Análise retrospectiva dos fatores associados à distribuição da tuberculose bovina no Estado do Rio de Janeiro. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, Belo Horizonte, v. 60, n. 3, p. 574-579, 2008.| Biblioteca(s): Embrapa Gado de Leite. |
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| 4. | | NASCIMENTO, M. G. F. do; CUNHA, C. P. da; JESUS, V. L. T. de; LIGNON, G. B.; NASCIMENTO, E. R. do. Levantamento de Brucella abortus em queijos minas frescal comercializados no Estado do Rio de Janeiro. Higiene Alimentar, São Paulo, v. 16, n. 101, p. 63-66, 2002.| Biblioteca(s): Embrapa Agroindústria de Alimentos. |
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Registro Completo
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Biblioteca(s): |
Embrapa Unidades Centrais. |
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Data corrente: |
25/04/2016 |
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Data da última atualização: |
25/04/2016 |
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Autoria: |
SANTOS, C. S. dos; JESUS, V. L. T. de; McINTOSH, D.; BERTO, B. P.; LOPES, C. W. G. |
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Afiliação: |
CAROLINE SPITZ DOS SANTOS, UFRRJ; VERA LÚCIA TEIXEIRA DE JESUS, UFRRJ; DOUGLAS McINTOSH, UFRRJ; BRUNO PEREIRA BERTO, UFRRJ; CARLOS WILSON GOMES LOPES, UFRRJ. |
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Título: |
Co-infection by Tritrichomonas foetus and Pentatrichomonas hominis in asymptomatic cats. |
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Ano de publicação: |
2015 |
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Fonte/Imprenta: |
Pesquisa Veterinária Brasileira, Brasília, DF, v. 35, n. 12, p. 980-988, dez. 2015. |
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Idioma: |
Inglês |
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Conteúdo: |
Tritrichomonas foetus, a parasite well known for its significance as a venereally transmitted pathogen in cattle, has been identified as a cause of chronic large bowel diarrhea in domestic cats in many countries of the world. In Brazil, several studies on the diagnosis of bovine trichomoniasis have been performed, but until now, no study was made regarding feline trichomoniasis. Thus, this is the first study to report the occurrence of T. foetus and Pentatrichomonas hominis in cats using morphological and molecular analysis. Feces from 77 cats were examined, four of which (5.2%) were positive for the presence of parabasalids. Morphological analysis of stained smears revealed piriform trophozoites showing the three anterior flagella, elongated nucleus and axostyle endingabruptly in fillet, characteristic of T. foetus. In scanning and transmission electron microscopy, identification characters similar to those previously reported for T. foetus were observed. The cultures containing trophozoites were submitted for molecular analysis, which resulted positive for T. foetus DNA using specific primers (TFR3 and TFR4), and all samples were positive and subjected to sequencing in which they showed 99.7-100% similarity with another isolate sequencing of T. foetus (JX960422). Although no trophozoite with consistent morphology of P. hominis has been visualized in the samples, differential diagnosis was performed using specific primers for P. hominis (TH3 and TH5) amplicon. In three of the four samples (3.89%) sequencing revealed 100% similarity when compared with another sequence of P. hominis deposited in Genbank (KC623939). Therefore, the present study revealed through the diagnostic techniques employed the simultaneous infection by T. foetus and P. hominis in the feces of cats. However, it was necessary to use more than one technique for the diagnosis of the co-infection. These results demonstrate the importance of a correct diagnosis to allow an appropriate treatment by the veterinarian. MenosTritrichomonas foetus, a parasite well known for its significance as a venereally transmitted pathogen in cattle, has been identified as a cause of chronic large bowel diarrhea in domestic cats in many countries of the world. In Brazil, several studies on the diagnosis of bovine trichomoniasis have been performed, but until now, no study was made regarding feline trichomoniasis. Thus, this is the first study to report the occurrence of T. foetus and Pentatrichomonas hominis in cats using morphological and molecular analysis. Feces from 77 cats were examined, four of which (5.2%) were positive for the presence of parabasalids. Morphological analysis of stained smears revealed piriform trophozoites showing the three anterior flagella, elongated nucleus and axostyle endingabruptly in fillet, characteristic of T. foetus. In scanning and transmission electron microscopy, identification characters similar to those previously reported for T. foetus were observed. The cultures containing trophozoites were submitted for molecular analysis, which resulted positive for T. foetus DNA using specific primers (TFR3 and TFR4), and all samples were positive and subjected to sequencing in which they showed 99.7-100% similarity with another isolate sequencing of T. foetus (JX960422). Although no trophozoite with consistent morphology of P. hominis has been visualized in the samples, differential diagnosis was performed using specific primers for P. hominis (TH3 and TH5) amplicon. In three of t... Mostrar Tudo |
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Palavras-Chave: |
Domestic cats; Gato doméstico. |
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Thesagro: |
Tritrichomonas Foetus. |
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Thesaurus NAL: |
Mixed infection; Pentatrichomonas hominis. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/142564/1/Co-infection-by-tritrichnomonas.pdf
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Marc: |
LEADER 02713naa a2200229 a 4500
001 2043968
005 2016-04-25
008 2015 bl uuuu u00u1 u #d
100 1 $aSANTOS, C. S. dos
245 $aCo-infection by Tritrichomonas foetus and Pentatrichomonas hominis in asymptomatic cats.
260 $c2015
520 $aTritrichomonas foetus, a parasite well known for its significance as a venereally transmitted pathogen in cattle, has been identified as a cause of chronic large bowel diarrhea in domestic cats in many countries of the world. In Brazil, several studies on the diagnosis of bovine trichomoniasis have been performed, but until now, no study was made regarding feline trichomoniasis. Thus, this is the first study to report the occurrence of T. foetus and Pentatrichomonas hominis in cats using morphological and molecular analysis. Feces from 77 cats were examined, four of which (5.2%) were positive for the presence of parabasalids. Morphological analysis of stained smears revealed piriform trophozoites showing the three anterior flagella, elongated nucleus and axostyle endingabruptly in fillet, characteristic of T. foetus. In scanning and transmission electron microscopy, identification characters similar to those previously reported for T. foetus were observed. The cultures containing trophozoites were submitted for molecular analysis, which resulted positive for T. foetus DNA using specific primers (TFR3 and TFR4), and all samples were positive and subjected to sequencing in which they showed 99.7-100% similarity with another isolate sequencing of T. foetus (JX960422). Although no trophozoite with consistent morphology of P. hominis has been visualized in the samples, differential diagnosis was performed using specific primers for P. hominis (TH3 and TH5) amplicon. In three of the four samples (3.89%) sequencing revealed 100% similarity when compared with another sequence of P. hominis deposited in Genbank (KC623939). Therefore, the present study revealed through the diagnostic techniques employed the simultaneous infection by T. foetus and P. hominis in the feces of cats. However, it was necessary to use more than one technique for the diagnosis of the co-infection. These results demonstrate the importance of a correct diagnosis to allow an appropriate treatment by the veterinarian.
650 $aMixed infection
650 $aPentatrichomonas hominis
650 $aTritrichomonas Foetus
653 $aDomestic cats
653 $aGato doméstico
700 1 $aJESUS, V. L. T. de
700 1 $aMcINTOSH, D.
700 1 $aBERTO, B. P.
700 1 $aLOPES, C. W. G.
773 $tPesquisa Veterinária Brasileira, Brasília, DF$gv. 35, n. 12, p. 980-988, dez. 2015.
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