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 | Acesso ao texto completo restrito à biblioteca da Embrapa Mandioca e Fruticultura. Para informações adicionais entre em contato com cnpmf.biblioteca@embrapa.br. |
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Registro Completo |
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Biblioteca(s): |
Embrapa Mandioca e Fruticultura. |
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Data corrente: |
21/03/2023 |
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Data da última atualização: |
01/06/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
DOCEMA, M. L.; MORAES, T. DE S.; ATTÍLIO, L. B.; MARQUES, J. P. R.; STIPP, L. C. L.; HARAKAVA, R.; ASTUA, J. de F.; BELASQUE JUNIOR, J.; AZEVEDO, R. A.; GAZIOLA, S. A.; MOURÃO FILHO, F. DE A. A. |
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Afiliação: |
MATHEUS LUÍS DOCEMA, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; TATIANA DE SOUZA MORAES, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; LÍSIA BORGES ATTÍLIO, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; JOÃO PAULO RODRIGUES MARQUES, UNIVERSIDADE DE SÃO PAULO; LILIANE CRISTINA LIBORIO STIPP, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; RICARDO HARAKAVA, INSTITUTO BIOLÓGICO; JULIANA DE FREITAS ASTUA, CNPMF; JOSÉ BELASQUE JUNIOR, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; RICARDO ANTUNES AZEVEDO, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; SALETE APARECIDA GAZIOLA, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ; FRANCISCO DE ASSIS ALVES MOURÃO FILHO, ESCOLA SUPERIOR DE AGRICULTURA LUIZ DE QUEIROZ. |
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Título: |
Transgenic 'Hamlin' sweet orange expressing csd1 or d4e1 genes exhibits decreased susceptibility to citrus canker disease. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Plant Cell, Tissue and Organ Culture, 2022. |
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DOI: |
https://doi.org/10.1007/s11240-022-02420-3 |
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Idioma: |
Inglês |
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Conteúdo: |
Currently, there are no resistant sweet orange cultivars to the citrus canker (cc) [Xanthomonas citri subsp. citri (Xcc)], and the production of transgenic plants resistant to cc can be an efficient solution in the search of improved cultivars. Several strategies can be applied in this scenario, including the expression of genes that code for antimicrobial peptides and resistance genes from Xcc-tolerant cultivars. We evaluated the reaction of 'Hamlin' sweet orange (Citrus sinensis L. Osbeck) transformed with a gene that codes for the D4E1 synthetic antimicrobial peptide, and ?Hamlin? sweet orange plants transformed with the gene that codes for a copper and zinc superoxide dismutase (csd1), isolated from Poncirus trifoliata, after inoculation with Xcc. Transgenic plants were propagated through grafting in ?Rangpur? lime (Citrus limonia Osbeck), and then inoculated with a Xcc suspension. Hydrogen peroxide production, activity of selected antioxidant enzymes and callose deposition were evaluated in the transgenic plants subjected to Xcc inoculation. The incidence and the severity of citrus canker were significantly reduced in several transgenic events, which also exhibited lower Xcc titers when compared with non-transgenic plants. Transgenic events which exhibited higher superoxide dismutase activities also showed increased accumulation of hydrogen peroxide in foliar tissues after Xcc inoculation. Epifluorescence microscopy analyses revealed a high callose deposition in the sieve tube elements of leaf tissues in some transgenic events. Callose accumulation is an important plant defense response to biotic stresses and, in csd1 transgenic lines, it was associated with lower Xcc populations. MenosCurrently, there are no resistant sweet orange cultivars to the citrus canker (cc) [Xanthomonas citri subsp. citri (Xcc)], and the production of transgenic plants resistant to cc can be an efficient solution in the search of improved cultivars. Several strategies can be applied in this scenario, including the expression of genes that code for antimicrobial peptides and resistance genes from Xcc-tolerant cultivars. We evaluated the reaction of 'Hamlin' sweet orange (Citrus sinensis L. Osbeck) transformed with a gene that codes for the D4E1 synthetic antimicrobial peptide, and ?Hamlin? sweet orange plants transformed with the gene that codes for a copper and zinc superoxide dismutase (csd1), isolated from Poncirus trifoliata, after inoculation with Xcc. Transgenic plants were propagated through grafting in ?Rangpur? lime (Citrus limonia Osbeck), and then inoculated with a Xcc suspension. Hydrogen peroxide production, activity of selected antioxidant enzymes and callose deposition were evaluated in the transgenic plants subjected to Xcc inoculation. The incidence and the severity of citrus canker were significantly reduced in several transgenic events, which also exhibited lower Xcc titers when compared with non-transgenic plants. Transgenic events which exhibited higher superoxide dismutase activities also showed increased accumulation of hydrogen peroxide in foliar tissues after Xcc inoculation. Epifluorescence microscopy analyses revealed a high callose deposition in the sie... Mostrar Tudo |
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Thesagro: |
Fruta Cítrica; Planta Transgênica. |
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Thesaurus Nal: |
Antimicrobial peptides; Citrus; Superoxide dismutase; Transgenic plants; Xanthomonas citri subsp. citri. |
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Categoria do assunto: |
G Melhoramento Genético |
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Marc: |
LEADER 02770naa a2200337 a 4500
001 2152545
005 2023-06-01
008 2022 bl uuuu u00u1 u #d
024 7 $ahttps://doi.org/10.1007/s11240-022-02420-3$2DOI
100 1 $aDOCEMA, M. L.
245 $aTransgenic 'Hamlin' sweet orange expressing csd1 or d4e1 genes exhibits decreased susceptibility to citrus canker disease.$h[electronic resource]
260 $c2022
520 $aCurrently, there are no resistant sweet orange cultivars to the citrus canker (cc) [Xanthomonas citri subsp. citri (Xcc)], and the production of transgenic plants resistant to cc can be an efficient solution in the search of improved cultivars. Several strategies can be applied in this scenario, including the expression of genes that code for antimicrobial peptides and resistance genes from Xcc-tolerant cultivars. We evaluated the reaction of 'Hamlin' sweet orange (Citrus sinensis L. Osbeck) transformed with a gene that codes for the D4E1 synthetic antimicrobial peptide, and ?Hamlin? sweet orange plants transformed with the gene that codes for a copper and zinc superoxide dismutase (csd1), isolated from Poncirus trifoliata, after inoculation with Xcc. Transgenic plants were propagated through grafting in ?Rangpur? lime (Citrus limonia Osbeck), and then inoculated with a Xcc suspension. Hydrogen peroxide production, activity of selected antioxidant enzymes and callose deposition were evaluated in the transgenic plants subjected to Xcc inoculation. The incidence and the severity of citrus canker were significantly reduced in several transgenic events, which also exhibited lower Xcc titers when compared with non-transgenic plants. Transgenic events which exhibited higher superoxide dismutase activities also showed increased accumulation of hydrogen peroxide in foliar tissues after Xcc inoculation. Epifluorescence microscopy analyses revealed a high callose deposition in the sieve tube elements of leaf tissues in some transgenic events. Callose accumulation is an important plant defense response to biotic stresses and, in csd1 transgenic lines, it was associated with lower Xcc populations.
650 $aAntimicrobial peptides
650 $aCitrus
650 $aSuperoxide dismutase
650 $aTransgenic plants
650 $aXanthomonas citri subsp. citri
650 $aFruta Cítrica
650 $aPlanta Transgênica
700 1 $aMORAES, T. DE S.
700 1 $aATTÍLIO, L. B.
700 1 $aMARQUES, J. P. R.
700 1 $aSTIPP, L. C. L.
700 1 $aHARAKAVA, R.
700 1 $aASTUA, J. de F.
700 1 $aBELASQUE JUNIOR, J.
700 1 $aAZEVEDO, R. A.
700 1 $aGAZIOLA, S. A.
700 1 $aMOURÃO FILHO, F. DE A. A.
773 $tPlant Cell, Tissue and Organ Culture, 2022.
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Registro original: |
Embrapa Mandioca e Fruticultura (CNPMF) |
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Biblioteca |
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Origem |
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Registro |
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Registro Completo
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Biblioteca(s): |
Embrapa Meio Ambiente. |
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Data corrente: |
17/04/2017 |
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Data da última atualização: |
29/06/2017 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
JESUS, K. R. E. de; MASSINI, K. C. |
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Afiliação: |
KATIA REGINA EVARISTO DE JESUS, CNPMA; K. C. MASSINI. |
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Título: |
Risk indicators of the nanoparticles-a decision making process aiming to support the nanomaterials development. |
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Ano de publicação: |
2016 |
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Fonte/Imprenta: |
In: ENCONTRO ANUAL DA SOCIEDADE BRASILEIRA DE PESQUISA EM MATERIAIS, 15., 2016, Campinas. [Abstracts...] Rio de Janeiro: Sociedade Brasileira de Pesquisas em Materiais, 2016. Resumo B62K. |
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Idioma: |
Inglês |
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Conteúdo: |
Nanotechnology allows us to develop high quality products with wide application in agriculture, environmental protection, among others. However, some questions arose about the safety of these products. Therefore, criteria to assess the safety of nanoproducts, especially for those that already are on the market has fundamental importance. The hazard or risk identification aims to identify potential adverse effects on the health or the environment that are associated with exposure to a biological, physical or chemical agent. Thus, this work presents some key indicators to assess the risk potential of nanoparticles available today. These indicators were developed by a multidisciplinary team of experts gathered research project coordinated and financed by Embrapa (Rede AgroNano) from technical variables formulated and grounded in the scientific literature and reports from international agencies. Risk indicators were validated remotely by experts from various fields of development and application of nanotechnology through a questionnaire developed according to the Delphi technique, which was made available on the website of Embrapa Environment. For the construction of this questionnaire was used Limesurvey, an open source software developed in PHP and using MySQL database. After statistical and technical analysis this information was validated by experts and stakeholders in technical workshops. According to them, these indicators represent the most critical aspects to the development of nanotechnology. These indicators are intended to help developers of these technologies to reassess the methodologies used for the development of nanotechnologies in order to mitigate a potential risk. MenosNanotechnology allows us to develop high quality products with wide application in agriculture, environmental protection, among others. However, some questions arose about the safety of these products. Therefore, criteria to assess the safety of nanoproducts, especially for those that already are on the market has fundamental importance. The hazard or risk identification aims to identify potential adverse effects on the health or the environment that are associated with exposure to a biological, physical or chemical agent. Thus, this work presents some key indicators to assess the risk potential of nanoparticles available today. These indicators were developed by a multidisciplinary team of experts gathered research project coordinated and financed by Embrapa (Rede AgroNano) from technical variables formulated and grounded in the scientific literature and reports from international agencies. Risk indicators were validated remotely by experts from various fields of development and application of nanotechnology through a questionnaire developed according to the Delphi technique, which was made available on the website of Embrapa Environment. For the construction of this questionnaire was used Limesurvey, an open source software developed in PHP and using MySQL database. After statistical and technical analysis this information was validated by experts and stakeholders in technical workshops. According to them, these indicators represent the most critical aspects to the develop... Mostrar Tudo |
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Thesagro: |
Risco. |
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Thesaurus NAL: |
Nanoparticles. |
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Categoria do assunto: |
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/161230/1/2016RA-059.pdf
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Marc: |
LEADER 02325nam a2200145 a 4500
001 2068546
005 2017-06-29
008 2016 bl uuuu u00u1 u #d
100 1 $aJESUS, K. R. E. de
245 $aRisk indicators of the nanoparticles-a decision making process aiming to support the nanomaterials development.$h[electronic resource]
260 $aIn: ENCONTRO ANUAL DA SOCIEDADE BRASILEIRA DE PESQUISA EM MATERIAIS, 15., 2016, Campinas. [Abstracts...] Rio de Janeiro: Sociedade Brasileira de Pesquisas em Materiais, 2016. Resumo B62K.$c2016
520 $aNanotechnology allows us to develop high quality products with wide application in agriculture, environmental protection, among others. However, some questions arose about the safety of these products. Therefore, criteria to assess the safety of nanoproducts, especially for those that already are on the market has fundamental importance. The hazard or risk identification aims to identify potential adverse effects on the health or the environment that are associated with exposure to a biological, physical or chemical agent. Thus, this work presents some key indicators to assess the risk potential of nanoparticles available today. These indicators were developed by a multidisciplinary team of experts gathered research project coordinated and financed by Embrapa (Rede AgroNano) from technical variables formulated and grounded in the scientific literature and reports from international agencies. Risk indicators were validated remotely by experts from various fields of development and application of nanotechnology through a questionnaire developed according to the Delphi technique, which was made available on the website of Embrapa Environment. For the construction of this questionnaire was used Limesurvey, an open source software developed in PHP and using MySQL database. After statistical and technical analysis this information was validated by experts and stakeholders in technical workshops. According to them, these indicators represent the most critical aspects to the development of nanotechnology. These indicators are intended to help developers of these technologies to reassess the methodologies used for the development of nanotechnologies in order to mitigate a potential risk.
650 $aNanoparticles
650 $aRisco
700 1 $aMASSINI, K. C.
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Embrapa Meio Ambiente (CNPMA) |
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