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| Registros recuperados : 14 | |
| 3. |  | ASCARI, I. J.; ALVES, N. G.; JASMIN, J.; LIMA, R. R.; QUINTAO, C. C. R.; OBERLENDER, G.; MORAES, E. A.; CAMARGO, L. S. de A. Addition of insulin-like growth factor I to the maturation medium of bovine oocytes subjected to heat shock: effects on the production of reactive oxygen species, mitochondrial activity and oocyte competence. Domestic Animal Endocrinology, v. 60, p. 50-60, 2017.| Biblioteca(s): Embrapa Gado de Leite. |
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| 4. | | ARAUJO, T. D.; JASMIN, J.; QUINTAO, C. C. R.; SOUZA, E. D.; VIANA, J. H. M.; CAMARGO, L. S. de A. Effect of DNA methylation inhibitor on heterochromatin in bovine embryos derived from heat-shocked oocytes In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 2016, Louisville. Reproduction, fertility and development: proceedings. Louisville: [s.n.], 2016.| Biblioteca(s): Embrapa Gado de Leite. |
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| 5. | | ASCARI, I. J.; ALVES, N. G.; CAMARGO, L. S. de A.; JASMIN, J.; QUINTAO, C. C. R.; OLIVEIRA, J. A. S.; ARAÚJO, T. D. Effect of melatonin on DNA fragmentation and in vitro maturation of bovine oocytes subjected to heat shock Animal Reproduction., v.12, n.3, Jul./Sept. 2015. Edição dos proceedings do 29º Annual Meeting of the Brazilian Embryo Technology Society, 2015, Gramado.| Biblioteca(s): Embrapa Gado de Leite. |
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| 6. | | JASMIN, J.; GONÇALVES, F. S.; ASCARI, I. J.; FONSECA, T. S.; CAMARGO, L. S. de A.; PETERS, V. M.; MENDEZ-OTERO, R. Optimization of in vitro development of mouse embryos using mesenchymal stem cells as feeder layers. Animal Reproduction, v. 11, n. 3, p. 463, 2014. Edição dos proceedings do 28º Annual Meeting of the Brazilian Embryo Technology Society, 2014, Natal.| Biblioteca(s): Embrapa Gado de Leite. |
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| 7. | | ALVES, N. G.; ASCARI, I. J.; CAMARGO, L. S. de A.; JASMIN, J.; QUINTAO, C. C. R.; OLIVEIRA, J. A. S.; SOUZA, E. D.; ASSUNÇÃO, C. M. A.; MENDES, V. R. A.; LIMA, R. R. Effect of melatonin on Embryo Quality of bovine oocytes subjected to heal shock. In: ANNUAL CONFERENCE OF THE INTERNATIONAL EMBRYO TRANSFER SOCIETY, 2016, Louisville. Reproduction, fertility and development: proceedings. Louisville: [s.n.], 2016.| Biblioteca(s): Embrapa Gado de Leite. |
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| 8. | | CARVALHEIRA, L. R.; JASMIN, J.; SANTOS, G. B.; GUIMARÃES, C. R.; ALVES, B. R. C.; MACHADO, F. S.; CAMPOS, M. M.; RODRIGUES, A. L. R.; NOGUEIRA, L. A. G.; CARVALHO, B. C. The effect of diets with different levels of degradable and undegradable protein in rumen on apoptosis in oocyte and in cumulus-oophurus cells of Girolando cows Animal Reproduction, v. 12, n. 3, 2015. Edição dos proceedings do 29º Annual Meeting of the Brazilian Embryo Technology Society, 2015, Gramado.| Biblioteca(s): Embrapa Gado de Leite. |
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| 9. | | ALVES, N. G.; ASCARI, I. J.; JASMIN, J.; QUINTAO, C. C. R.; SOUZA, J. F.; TORRES, G.; JUNQUEIRA, F. B.; SILVA, D. R.; CAMPOS, J. P. A.; FARIA, L. R.; PAULA, F. A. De P.; CAMARGO, L. S. de A. Effect of insulin-like growth factor-l on embryo quality of bovine oocytes subjected to theat shock In: INTERNATIONAL CONGRESS ON ANIMAL REPRODUCTION, 18., 2016, Tours. Abstracts... Tours: Society for Reproductive Biology, 2016. p. 247-248.| Biblioteca(s): Embrapa Gado de Leite. |
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| 10. | | ASCARI, I. J.; ALVES, N. G.; JASMIN, J.; QUINTAO, C. C. R.; SOUZA, E. D. De; SOUZA, J. F.; BATISTA, R. I. T. P.; MENDES, V. R. A.; JUNQUEIRA, F.; CAMARGO, L. S. de A. Efeito do IGF-I na maturação in vitro de oócitos bovinos submetidos ao choque técnico In: REUNIÃO ANUAL DA SOCIEDADE BRASILEIRA DE TECNOLOGIA DE EMBRIÕES, 30., 2016, Foz do Iguaçu. Anais... Foz do Iguaçu: Sociedade Brasileira de Tecnologia de Embriões, 2016. 1| Biblioteca(s): Embrapa Gado de Leite. |
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| 13. | | GAMA-RODRIGUES, A. C. da; BARROS, N. F. de; GAMA-RODRIGUES, E. F. da; FREITAS, M. S. M.; VIANA, A. P.; JASMIN, J. M.; MARCIANO, C. R.; CARNEIRO, J. G. de A. (ed.). Sistemas agroflorestais: bases científicas para o desenvolvimento sustentável. Campos dos Goytacazes, RJ: Universidade Estadual do Norte Fluminense Darcy Ribeiro, 2006. 365 p.| Biblioteca(s): Embrapa Acre; Embrapa Agrobiologia; Embrapa Agroindústria de Alimentos; Embrapa Agropecuária Oeste; Embrapa Algodão; Embrapa Amapá; Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Arroz e Feijão; Embrapa Caprinos e Ovinos; Embrapa Clima Temperado; Embrapa Gado de Corte; Embrapa Gado de Leite; Embrapa Hortaliças; Embrapa Instrumentação; Embrapa Mandioca e Fruticultura; Embrapa Meio-Norte; Embrapa Pantanal; Embrapa Pecuária Sudeste; Embrapa Pecuária Sul; Embrapa Rondônia; Embrapa Semiárido; Embrapa Soja; Embrapa Solos / UEP-Recife; Embrapa Suínos e Aves; Embrapa Tabuleiros Costeiros; Embrapa Unidades Centrais; Embrapa Uva e Vinho. MenosEmbrapa Acre; Embrapa Agrobiologia; Embrapa Agroindústria de Alimentos; Embrapa Agropecuária Oeste; Embrapa Algodão; Embrapa Amapá; Embrapa Amazônia Ocidental; Embrapa Amazônia Oriental; Embrapa Arroz e Feijão; Embrapa Caprinos e Ovinos... Mostrar Todas |
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| 14. | | ACARI, I. J.; ALVES, N. G.; JASMIN, J.; QUINTAO, C. C. R.; SOUZA, J. F.; TORRES, G.; GONÇALVES, F.; JUNQUEIRA, F. B.; SILVA, D. R.; CAMPOS, J. P. A.; FARIA, L. R.; PAULA, F. A. De P.; CAMARGO, L. S. de A. Antioxidant effects of IGF-I on bovine oocytes subjected to heat shock In: INTERNATIONAL CONGRESS ON ANIMAL REPRODUCTION, 18., 2016, Tours. Abstracts... Tours: Society for Reproductive Biology, 2016. p. 246-247.| Biblioteca(s): Embrapa Gado de Leite. |
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| Registros recuperados : 14 | |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Gado de Leite. Para informações adicionais entre em contato com cnpgl.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Gado de Leite. |
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Data corrente: |
26/12/2018 |
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Data da última atualização: |
24/01/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
ASCARI, I. J.; MARTINS, S. C.; CAMARGO, L. S. de A.; MENDEZ-OTERO, R.; JASMIN. |
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Afiliação: |
LUIZ SERGIO DE ALMEIDA CAMARGO, CNPGL. |
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Título: |
Development of bovine embryos in vitro in coculture with murine mesenchymal stem cells and embryonic fibroblasts. |
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Ano de publicação: |
2018 |
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Fonte/Imprenta: |
Molecular Biology Reports, v. 45, n. 6, p. 1827-1837, 2018. |
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DOI: |
10.1007/s11033-018-4329-y |
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Idioma: |
Inglês |
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Conteúdo: |
Abstract Despite the progress on development of new culture media, in vitro-produced embryos still display lower quality when compared to the in vivo-produced counterparts. Coculture has been reconsidered as an alternative to improve embryo quality. Mesenchymal stem cells (MSC) and murine embryonic fibroblasts (MEF) have been extensively used as feeder layers due to their capacity to release growth factors. In the present study we investigated the effect of these feeder layers in oocyte maturation and/or embryo development under in vitro conditions. Oocytes were matured in control (CTRL) conditions or in coculture with MSC or MEF. In vitro fertilization and embryo culture until fourth day were performed in CTRL condition for all groups. Embryos from fourth day on were then cultured until the eighth day in CTRL or in coculture system. No significant differences for metaphase II stage and apoptosis in oocytes were found among the groups. There was also no difference among the groups when we evaluated blastocyst formation on the seventh and eighth day, with exception of a higher hatched blastocyst rate in the group maturated and cultivated in CTRL condition when compared to the group matured and cocultured with MSC. Also no difference was observed in the number of cells in the whole embryos, in the inner cell mass, in the trophoblast and at apoptotic stage on the eighth day. We conclude that coculture with MSC or MEF during maturation and/or embryo development do not enhance the in vitro production of bovine embryos. MenosAbstract Despite the progress on development of new culture media, in vitro-produced embryos still display lower quality when compared to the in vivo-produced counterparts. Coculture has been reconsidered as an alternative to improve embryo quality. Mesenchymal stem cells (MSC) and murine embryonic fibroblasts (MEF) have been extensively used as feeder layers due to their capacity to release growth factors. In the present study we investigated the effect of these feeder layers in oocyte maturation and/or embryo development under in vitro conditions. Oocytes were matured in control (CTRL) conditions or in coculture with MSC or MEF. In vitro fertilization and embryo culture until fourth day were performed in CTRL condition for all groups. Embryos from fourth day on were then cultured until the eighth day in CTRL or in coculture system. No significant differences for metaphase II stage and apoptosis in oocytes were found among the groups. There was also no difference among the groups when we evaluated blastocyst formation on the seventh and eighth day, with exception of a higher hatched blastocyst rate in the group maturated and cultivated in CTRL condition when compared to the group matured and cocultured with MSC. Also no difference was observed in the number of cells in the whole embryos, in the inner cell mass, in the trophoblast and at apoptotic stage on the eighth day. We conclude that coculture with MSC or MEF during maturation and/or embryo development do not enhance th... Mostrar Tudo |
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Palavras-Chave: |
Bovine; In vitro embryonic development; Mesenchymal stem cells; Murine embryonic fibroblast. |
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Thesaurus NAL: |
Coculture. |
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Categoria do assunto: |
L Ciência Animal e Produtos de Origem Animal |
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Marc: |
LEADER 02308naa a2200241 a 4500
001 2102511
005 2023-01-24
008 2018 bl uuuu u00u1 u #d
024 7 $a10.1007/s11033-018-4329-y$2DOI
100 1 $aASCARI, I. J.
245 $aDevelopment of bovine embryos in vitro in coculture with murine mesenchymal stem cells and embryonic fibroblasts.$h[electronic resource]
260 $c2018
520 $aAbstract Despite the progress on development of new culture media, in vitro-produced embryos still display lower quality when compared to the in vivo-produced counterparts. Coculture has been reconsidered as an alternative to improve embryo quality. Mesenchymal stem cells (MSC) and murine embryonic fibroblasts (MEF) have been extensively used as feeder layers due to their capacity to release growth factors. In the present study we investigated the effect of these feeder layers in oocyte maturation and/or embryo development under in vitro conditions. Oocytes were matured in control (CTRL) conditions or in coculture with MSC or MEF. In vitro fertilization and embryo culture until fourth day were performed in CTRL condition for all groups. Embryos from fourth day on were then cultured until the eighth day in CTRL or in coculture system. No significant differences for metaphase II stage and apoptosis in oocytes were found among the groups. There was also no difference among the groups when we evaluated blastocyst formation on the seventh and eighth day, with exception of a higher hatched blastocyst rate in the group maturated and cultivated in CTRL condition when compared to the group matured and cocultured with MSC. Also no difference was observed in the number of cells in the whole embryos, in the inner cell mass, in the trophoblast and at apoptotic stage on the eighth day. We conclude that coculture with MSC or MEF during maturation and/or embryo development do not enhance the in vitro production of bovine embryos.
650 $aCoculture
653 $aBovine
653 $aIn vitro embryonic development
653 $aMesenchymal stem cells
653 $aMurine embryonic fibroblast
700 1 $aMARTINS, S. C.
700 1 $aCAMARGO, L. S. de A.
700 1 $aMENDEZ-OTERO, R.
700 1 $aJASMIN
773 $tMolecular Biology Reports$gv. 45, n. 6, p. 1827-1837, 2018.
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