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Registro Completo |
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Biblioteca(s): |
Embrapa Algodão. |
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Data corrente: |
12/12/2012 |
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Data da última atualização: |
13/12/2012 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
JERÔNIMO, J. F.; FRANÇA, P. R. C. de; SILVA, O. R. R. F.; ALMEIDA, F. de A. C.; SOFIATTI, V. |
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Afiliação: |
Jeane Ferreira Jerônimo, Doutora em Engenharia Agrícola; Pablo Radamés Cabral de França, Bolsista da Embrapa Algodão, doutorando em Agronomia pelo PPGA/UFPB; ODILON RENY RIBEIRO FERREIRA SILVA, CNPA; Francisco de Assis Cardoso Almeida, Professor da UFCG, doutor em Agronomia; VALDINEI SOFIATTI, CNPA. |
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Título: |
Comparação dos descaroçadores de 25 e 50 serras na qualidade tecnológica da fibra de algodão. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
CONGRESSO BRASILEIRO DE MAMONA, 5.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 2.; FÓRUM CAPIXABA DE PINHÃO-MANSO, 1., 2012, Guarapari. Desafios e Oportunidades: anais. Campina Grande: Embrapa Algodão, 2012. |
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Páginas: |
p. 338 |
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Idioma: |
Português |
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Palavras-Chave: |
DESCAROÇADOR; FIBRAS. |
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Thesagro: |
Algodão; Gossypium Hirsutum. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/72145/1/MEC-009-P.166.pdf
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Marc: |
LEADER 00780nam a2200205 a 4500
001 1942294
005 2012-12-13
008 2012 bl uuuu u00u1 u #d
100 1 $aJERÔNIMO, J. F.
245 $aComparação dos descaroçadores de 25 e 50 serras na qualidade tecnológica da fibra de algodão.
260 $aCONGRESSO BRASILEIRO DE MAMONA, 5.; SIMPÓSIO INTERNACIONAL DE OLEAGINOSAS ENERGÉTICAS, 2.; FÓRUM CAPIXABA DE PINHÃO-MANSO, 1., 2012, Guarapari. Desafios e Oportunidades: anais. Campina Grande: Embrapa Algodão$c2012
300 $ap. 338
650 $aAlgodão
650 $aGossypium Hirsutum
653 $aDESCAROÇADOR
653 $aFIBRAS
700 1 $aFRANÇA, P. R. C. de
700 1 $aSILVA, O. R. R. F.
700 1 $aALMEIDA, F. de A. C.
700 1 $aSOFIATTI, V.
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Registro original: |
Embrapa Algodão (CNPA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Amazônia Ocidental. Para informações adicionais entre em contato com cpaa.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Amazônia Ocidental. |
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Data corrente: |
23/01/2013 |
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Data da última atualização: |
25/03/2015 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 2 |
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Autoria: |
MUNIZ, C. R.; FREIRE, F. das C. O.; VIANA, F. M. P.; CARDOSO, J. E.; CORREIA, D.; JALINK, H.; KEMA, G. H. J.; SILVA, G. F. da; GUEDES, M. I. F. |
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Afiliação: |
CELLI RODRIGUES MUNIZ, CNPAT; FRANCISCO DAS CHAGAS O FREIRE, CNPAT; FRANCISCO MARTO PINTO VIANA, CNPAT; JOSE EMILSON CARDOSO, CNPAT; DIVA CORREIA, CNPAT; WAGENINGEN UNIVERSITY GREENHOUSE HORTICULTURE; PLANT RESEARCH INTERNATIONAL; GILVAN FERREIRA DA SILVA, CPAA; UNIVERSIDADE ESTADUAL DO CEARÁ. |
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Título: |
Polyclonal antibody-based ELISA in combination with specific PCR amplification of internal transcribed spacer regions for the detection and quantitation of Lasiodiplodia theobromae, causal agent of gummosis in cashew nut plants. |
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Ano de publicação: |
2012 |
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Fonte/Imprenta: |
Annals of Applied Biology, v. 160, n. 3, p. 217-224, 2012. |
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ISSN: |
0003-4746 |
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DOI: |
10.1111/j.1744-7348.2012.00534.x |
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Idioma: |
Português |
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Conteúdo: |
Members of Botryosphaeriaceae family are associated with serious diseases in different plants across the world. In cashew nut plants (Anacardium occidentale), the fungus Lasiodiplodia theobromae causes a severe group of symptoms related to gummosis that results in decreased nut production. The aim of this work was to develop an indirect enzyme-linked immunosorbent assay (ELISA) with sufficient sensitivity and specificity to detect the fungus both in vitro and in planta (artificially and naturally infected) and to increase the detection specificity within the fungi group using primers specific for the internal transcribed spacer (ITS) sequences. A collection of L. theobromae isolates was obtained, and antisera against the fungus were raised in rabbits. Cross-reactivity against Neofusicoccum sp., Colletotrichum gloeosporioides, Phomopsis anacardii and Pestalotiopsis guepinii was examined. Naturally and artificially infected vegetal material were employed in the ELISAs. The fungi ITS sequences were determined, and single nucleotide polymorphisms were identified and used for primer design. For the naturally infected plants, there was an approximately fourfold variation in the absorbance values. Some positive readings for asymptomatic samples were detected. For the artificially infected samples, an ELISA-based weekly timecourse analysis was conducted, and the values for samples from 0 and 7 days were lower than the threshold value. Beginning on day 14, the infection could be detected, with rates varying from 40% on day 14 to 80% on day 21 and 100% by the end of the experiment. The ITS sequencing revealed few polymorphisms among the L. theobromae isolates, but for C. gloeosporioides, P. anacardii, P. guepinii and Neofusicoccum sp., the sequences were sufficient to permit reliable discrimination. The feasibility of ELISA as an early detection technique to assist in gummosis management was demonstrated. PCR amplification based on ITS regions increases and complements serological specificity. MenosMembers of Botryosphaeriaceae family are associated with serious diseases in different plants across the world. In cashew nut plants (Anacardium occidentale), the fungus Lasiodiplodia theobromae causes a severe group of symptoms related to gummosis that results in decreased nut production. The aim of this work was to develop an indirect enzyme-linked immunosorbent assay (ELISA) with sufficient sensitivity and specificity to detect the fungus both in vitro and in planta (artificially and naturally infected) and to increase the detection specificity within the fungi group using primers specific for the internal transcribed spacer (ITS) sequences. A collection of L. theobromae isolates was obtained, and antisera against the fungus were raised in rabbits. Cross-reactivity against Neofusicoccum sp., Colletotrichum gloeosporioides, Phomopsis anacardii and Pestalotiopsis guepinii was examined. Naturally and artificially infected vegetal material were employed in the ELISAs. The fungi ITS sequences were determined, and single nucleotide polymorphisms were identified and used for primer design. For the naturally infected plants, there was an approximately fourfold variation in the absorbance values. Some positive readings for asymptomatic samples were detected. For the artificially infected samples, an ELISA-based weekly timecourse analysis was conducted, and the values for samples from 0 and 7 days were lower than the threshold value. Beginning on day 14, the infection could be dete... Mostrar Tudo |
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Palavras-Chave: |
Anacardium occidentale L; Cashew agri-industry; Endophytic fungi; Immunodetection. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 03001naa a2200289 a 4500
001 1946100
005 2015-03-25
008 2012 bl uuuu u00u1 u #d
022 $a0003-4746
024 7 $a10.1111/j.1744-7348.2012.00534.x$2DOI
100 1 $aMUNIZ, C. R.
245 $aPolyclonal antibody-based ELISA in combination with specific PCR amplification of internal transcribed spacer regions for the detection and quantitation of Lasiodiplodia theobromae, causal agent of gummosis in cashew nut plants.
260 $c2012
520 $aMembers of Botryosphaeriaceae family are associated with serious diseases in different plants across the world. In cashew nut plants (Anacardium occidentale), the fungus Lasiodiplodia theobromae causes a severe group of symptoms related to gummosis that results in decreased nut production. The aim of this work was to develop an indirect enzyme-linked immunosorbent assay (ELISA) with sufficient sensitivity and specificity to detect the fungus both in vitro and in planta (artificially and naturally infected) and to increase the detection specificity within the fungi group using primers specific for the internal transcribed spacer (ITS) sequences. A collection of L. theobromae isolates was obtained, and antisera against the fungus were raised in rabbits. Cross-reactivity against Neofusicoccum sp., Colletotrichum gloeosporioides, Phomopsis anacardii and Pestalotiopsis guepinii was examined. Naturally and artificially infected vegetal material were employed in the ELISAs. The fungi ITS sequences were determined, and single nucleotide polymorphisms were identified and used for primer design. For the naturally infected plants, there was an approximately fourfold variation in the absorbance values. Some positive readings for asymptomatic samples were detected. For the artificially infected samples, an ELISA-based weekly timecourse analysis was conducted, and the values for samples from 0 and 7 days were lower than the threshold value. Beginning on day 14, the infection could be detected, with rates varying from 40% on day 14 to 80% on day 21 and 100% by the end of the experiment. The ITS sequencing revealed few polymorphisms among the L. theobromae isolates, but for C. gloeosporioides, P. anacardii, P. guepinii and Neofusicoccum sp., the sequences were sufficient to permit reliable discrimination. The feasibility of ELISA as an early detection technique to assist in gummosis management was demonstrated. PCR amplification based on ITS regions increases and complements serological specificity.
653 $aAnacardium occidentale L
653 $aCashew agri-industry
653 $aEndophytic fungi
653 $aImmunodetection
700 1 $aFREIRE, F. das C. O.
700 1 $aVIANA, F. M. P.
700 1 $aCARDOSO, J. E.
700 1 $aCORREIA, D.
700 1 $aJALINK, H.
700 1 $aKEMA, G. H. J.
700 1 $aSILVA, G. F. da
700 1 $aGUEDES, M. I. F.
773 $tAnnals of Applied Biology$gv. 160, n. 3, p. 217-224, 2012.
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