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Registro Completo |
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
28/10/2010 |
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Data da última atualização: |
28/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
ABREU, J. T.; MOURÃO, M. M.; SANTOS, C. E.; VELOSO, C. J. M.; RESENDE, J. S.; FLATSCHART, R. B.; FOLGUERAS-FLATSCHAR, A. V.; JUNIOR, S. N.; SANTORO, M. M.; MENDES, A. C. R.; FRANCO, G. R.; SILVA, A.; CAMPOS, A. B.; FERNANDEZ, S. |
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Afiliação: |
AUREA VALADARES FOLGUERAS FLATSCHAR, CNPMS. |
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Título: |
Molecular studies of the brazilian infectious bronchitis virus isolates. |
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Ano de publicação: |
2010 |
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Fonte/Imprenta: |
Revista Brasileira de Ciência Avícola, Campinas, v. 12, n. 2, p. 107-110, 2010. |
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DOI: |
10.1590/S1516-635X2010000200005 |
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Idioma: |
Inglês |
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Conteúdo: |
Avian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading to the conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais before the official approval of vaccination (1980). MenosAvian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading to the conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais bef... Mostrar Tudo |
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Thesagro: |
Genética; Virus. |
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Categoria do assunto: |
-- |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/32305/1/Molecular-studies.pdf
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Marc: |
LEADER 02461naa a2200313 a 4500
001 1865545
005 2018-05-28
008 2010 bl uuuu u00u1 u #d
024 7 $a10.1590/S1516-635X2010000200005$2DOI
100 1 $aABREU, J. T.
245 $aMolecular studies of the brazilian infectious bronchitis virus isolates.$h[electronic resource]
260 $c2010
520 $aAvian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading to the conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais before the official approval of vaccination (1980).
650 $aGenética
650 $aVirus
700 1 $aMOURÃO, M. M.
700 1 $aSANTOS, C. E.
700 1 $aVELOSO, C. J. M.
700 1 $aRESENDE, J. S.
700 1 $aFLATSCHART, R. B.
700 1 $aFOLGUERAS-FLATSCHAR, A. V.
700 1 $aJUNIOR, S. N.
700 1 $aSANTORO, M. M.
700 1 $aMENDES, A. C. R.
700 1 $aFRANCO, G. R.
700 1 $aSILVA, A.
700 1 $aCAMPOS, A. B.
700 1 $aFERNANDEZ, S.
773 $tRevista Brasileira de Ciência Avícola, Campinas$gv. 12, n. 2, p. 107-110, 2010.
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Registro original: |
Embrapa Milho e Sorgo (CNPMS) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Milho e Sorgo. Para informações adicionais entre em contato com cnpms.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Milho e Sorgo. |
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Data corrente: |
26/01/2006 |
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Data da última atualização: |
28/05/2018 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
CARNEIRO, N. P.; HUGHES, P. A.; LARKINS, B. A. |
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Afiliação: |
NEWTON PORTILHO CARNEIRO, CNPMS. |
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Título: |
The eEF1A gene family is differentially expressed in maize endosperm. |
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Ano de publicação: |
1999 |
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Fonte/Imprenta: |
Plant Molecular Biology, Dordrecht, v. 41, n. 6, p. 801-813, 1999. |
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Idioma: |
Inglês |
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Conteúdo: |
The elongation factor protein eEF1A appears to be a multifunctional protein in eukaryotes, where it serves as a protein synthesis factor as well as a cytoskeletal protein. In maize endosperm, the eEF1A concentration is highly correlated with lysine content, and eEF1A synthesis is increased in opaque2 mutants compared to wild type plants. To investigate the basis for the increased synthesis of eEF1A in opaque2, genes encoding this protein were characterized and their relative level of expression in endosperm and other tissues was investigated. Maize contains 10 to 15 eEF1A genes that are nearly identical in nucleotide and amino acid sequences. However, these genes can be distinguished based on their 3' non-coding sequences, which are less conserved. By screening endosperm and seedling cDNA libraries, it was shown that the relative level of transcripts of maize eEF1A genes varies in different tissues. At least five genes are transcribed in the endosperm, with 2 accounting for ca. 80% of the RNA transcripts. The expression of several genes is enhanced in opaque2 endosperm, although the significance of this is unclear. Nucleotide sequence data have been submitted to the EMBL/DDBJ/GenBank databases under the accession numbers AF136823 to AF136829 for eEF1Aa, eEF1Ab, eEF1Ac, eEF1Ad, eEF1Ae, eEF1Af and eEF1Ag, respectively.. |
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Palavras-Chave: |
Maize. |
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Thesagro: |
Milho; Zea mays. |
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Thesaurus NAL: |
endosperm. |
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Categoria do assunto: |
S Ciências Biológicas |
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Marc: |
LEADER 01879naa a2200193 a 4500
001 1489191
005 2018-05-28
008 1999 bl uuuu u00u1 u #d
100 1 $aCARNEIRO, N. P.
245 $aThe eEF1A gene family is differentially expressed in maize endosperm.$h[electronic resource]
260 $c1999
520 $aThe elongation factor protein eEF1A appears to be a multifunctional protein in eukaryotes, where it serves as a protein synthesis factor as well as a cytoskeletal protein. In maize endosperm, the eEF1A concentration is highly correlated with lysine content, and eEF1A synthesis is increased in opaque2 mutants compared to wild type plants. To investigate the basis for the increased synthesis of eEF1A in opaque2, genes encoding this protein were characterized and their relative level of expression in endosperm and other tissues was investigated. Maize contains 10 to 15 eEF1A genes that are nearly identical in nucleotide and amino acid sequences. However, these genes can be distinguished based on their 3' non-coding sequences, which are less conserved. By screening endosperm and seedling cDNA libraries, it was shown that the relative level of transcripts of maize eEF1A genes varies in different tissues. At least five genes are transcribed in the endosperm, with 2 accounting for ca. 80% of the RNA transcripts. The expression of several genes is enhanced in opaque2 endosperm, although the significance of this is unclear. Nucleotide sequence data have been submitted to the EMBL/DDBJ/GenBank databases under the accession numbers AF136823 to AF136829 for eEF1Aa, eEF1Ab, eEF1Ac, eEF1Ad, eEF1Ae, eEF1Af and eEF1Ag, respectively..
650 $aendosperm
650 $aMilho
650 $aZea mays
653 $aMaize
700 1 $aHUGHES, P. A.
700 1 $aLARKINS, B. A.
773 $tPlant Molecular Biology, Dordrecht$gv. 41, n. 6, p. 801-813, 1999.
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