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| Registros recuperados : 41 | |
| 10. |  | FEITOSA, H. O.; JUNQUEIRA, R. M.; GUERRA, J. G. M.; TEIXEIRA, M. G.; RESENDE, F. V.; CARVALHO, C. M. Produtividade do alho em diferentes regiões do Rio de Janeiro, sob sistema orgâncio de produção. Agropecuária Técnica, v. 30, n. 1, p. 13-20, 2009.| Biblioteca(s): Embrapa Agrobiologia. |
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| 14. | | JUNQUEIRA, R. M.; MOURA, A. F. G. de; BUSQUET, R. N. B.; RIBEIRO, R. de L. D.; ALMEIDA, D. L. de; GUERRA, J. G. M. Influência de diferentes tipos de cobertura de solo no desempenho de figueira (Ficus carica L.) em sistema orgânico de produção. In: CONGRESSO DE PESQUISA, 2., JORNADA DE INICIAÇÃO CIENTÍFICAS DA UFRural/RJ, 14., 2004, Seropédica, RJ. Anais... Seropédica: Universidade Federal Rural do Rio de Janeiro, 2004. v. 14. p. 191-193. CD ROM. Área de Agronomia. JIC 281.pdf.| Biblioteca(s): Embrapa Agrobiologia. |
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| 15. | | OLIVEIRA, F. L. de; RIBAS, R. G. T.; JUNQUEIRA, R. M.; PADOVAN, M. P.; ALMEIDA, D. L. de; RIBEIRO, R. de L. D.; GUERRA, J. G. M. Efeito da Adubação Verde no desempenho do consórcio repolho-rabanete sob manejo orgânico. Seropédica: Embrapa Agrobiologia, 2003. 6 p. Trabalho apresentado no 43 Congresso Brasileiro de Olericultura, Recife, 2003.| Biblioteca(s): Embrapa Agrobiologia. |
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| 16. | | OLIVEIRA, F. L. de; RIBAS, R. G. T.; JUNQUEIRA, R. M.; PADOVAN, M. P.; GUERRA, J. G. M.; ALMEIDA, D. L. de; RIBEIRO, R. de L. D. Efeito da adubação verde no desempenho do consórcio repolho-rabanete sob manejo orgânico. Horticultura Brasileira, Brasília, v. 21, n. 2, jul. 2003. Suplemento 2. Trabalho apresentado no 43º Congresso Brasileiro de Olericultura, 2003. Publicado também como resumo em: Horticultura Brasileira, Brasília, v. 21, n. 2, p. 268, jul. 2003. Suplemento 1.| Biblioteca(s): Embrapa Hortaliças. |
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| 17. | | OLIVEIRA, F. L. de; RIBAS, R. G. T.; JUNQUEIRA, R. M.; PADOVAN, M. P.; GUERRA, J. G. M.; ALMEIDA, D. L. de; RIBEIRO, R. de L. D. Efeito da adubação verde no desempenho do consórcio repolho-rabanete sob manejo orgânico. In: CONGRESSO BRASILEIRO DE OLERICULTURA, 43., 2003, Recife. Horticultura Brasileira, Brasília, v. 21, n. 2, p. 268, 2003. Resumo. Suplemento.| Biblioteca(s): Embrapa Agrobiologia. |
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| 18. | | OLIVEIRA, F. L. de; RIBAS, R. G. T.; JUNQUEIRA, R. M.; GUERRA, J. G. M.; ALMEIDA, D. L. de; RIBEIRO, R. de L. D. Efeito da cobertura com aveia preta e do cultivo consorciado com crotalária, na produção do Inhame (Taro) orgânico. Seropédica: Embrapa Agrobiologia, 2003. 6 p. Trabalho apresentado no 43 Congresso Brasileiro De Olericultura, Recife, 2003.| Biblioteca(s): Embrapa Agrobiologia. |
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| 19. | | OLIVEIRA, F. L. de; RIBAS, R. G. T.; JUNQUEIRA, R. M.; GUERRA, J. G. M.; ALMEIDA, D. L. de; RIBEIRO, R. de L. B. Efeito da cobertura morta com resíduos de aveia preta e do cultivo consorciado com crotalária na produção do inhame (taro) orgânico. Horticultura Brasileira, Brasília, v. 21, n. 2, jul. 2003. Suplemento 2. Trabalho apresentado no 43º Congresso Brasileiro de Olericultura, 2003. Publicado também como resumo em: Horticultura Brasileira, Brasília, v. 21, n. 2, p. 272, jul. 2003. Suplemento 1.| Biblioteca(s): Embrapa Hortaliças. |
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| 20. | | OLIVEIRA, F. L. de; RIBAS, R. G. T.; JUNQUEIRA, R. M.; GUERRA, J. G. M.; ALMEIDA, D. L. de; RIBEIRO, R. de L. D. Efeito da cobertura morta com resíduos de aveia preta e do cultivo consorciado com crotalária, na produção do inhame (Taro) orgânico. In: CONGRESSO BRASILEIRO DE OLERICULTURA, 43., 2003, Recife. Horticultura Brasileira, Brasília, v. 21, n. 2, p. 272, 2003. Resumo. Suplemento.| Biblioteca(s): Embrapa Agrobiologia. |
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| Registros recuperados : 41 | |
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Registro Completo
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Biblioteca(s): |
Embrapa Algodão; Embrapa Arroz e Feijão; Embrapa Recursos Genéticos e Biotecnologia. |
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Data corrente: |
15/09/2022 |
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Data da última atualização: |
16/09/2022 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Circulação/Nível: |
A - 1 |
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Autoria: |
FERREIRA, A. L.; FARIA, J. C. de; MOURA, M. da C.; ZAIDEM, A. L. de M.; PIZETTA, C. S. R.; FREITAS, E. de O.; COELHO, G. R. C.; SILVA, J. F. A. e; BARRIGOSSI, J. A. F.; HOFFMANN, L. V.; SOUZA, T. L. P. O. de; ARAGÃO, F. J. L.; PINHEIRO, P. V. |
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Afiliação: |
AMANDA LOPES FERREIRA, bolsista CNPAF; JOSIAS CORREA DE FARIA, CNPAF; MATHEUS DA COSTA MOURA, UNIVERSIDADE FEDERAL DE GOIÁS; ANTÔNIA LOPES DE MENDONÇA ZAIDEM, UNIVERSIDADE FEDERAL DE GOIÁS; CAROLINA SENHORINHO RAMALHO PIZETTA; ELÍNEA DE OLIVEIRA FREITAS; GESIMARIA RIBEIRO COSTA COELHO, CNPAF; JOSE FRANCISCO ARRUDA E SILVA, CNPAF; JOSE ALEXANDRE F BARRIGOSSI, CNPAF; LUCIA VIEIRA HOFFMANN, CNPA; THIAGO LIVIO PESSOA OLIV DE SOUZA, CNPAF; FRANCISCO JOSE LIMA ARAGAO, Cenargen; PATRICIA VALLE PINHEIRO, CNPAF. |
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Título: |
Whitefly-tolerant transgenic common bean (Phaseolus vulgaris) line. |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Frontiers in Plant Science, v. 13, 984804, Aug. 2022. |
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ISSN: |
1664-462X |
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DOI: |
https://doi.org/10.3389/fpls.2022.984804 |
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Idioma: |
Inglês |
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Conteúdo: |
Common bean (Phaseolus vulgaris L.) is a staple food in Brazil with both nutritional and socioeconomic importance. As an orphan crop, it has not received as much research attention as the commodity crops. Crop losses are strongly related to virus diseases transmitted by the whitefly Bemisia tabaci, one of the most important agricultural pests in the world. The main method of managing whitefly-transmitted viruses has been the application of insecticides to reduce vector populations. Compared to chemical vector control, a more sustainable strategy for managing insect-borne viruses is the development of resistant/tolerant cultivars. RNA interference has been applied to develop plant lines resistant to the whitefly in other species, such as tomato, lettuce and tobacco. Still, no whitefly-resistant plant has been made commercially available to date. Common bean is a recalcitrant species to in vitro regeneration; therefore, stable genetic transformation of this plant has been achieved only at low frequencies (<1%) using particle bombardment. In the present work, two transgenic common bean lines were obtained with an intron-hairpin construct to induce post-transcriptional gene silencing against the B. tabaci vATPase (Bt-vATPase) gene, with stable expression of siRNA. Northern blot analysis revealed the presence of bands of expected size for siRNA in leaf samples of the line Bt-22.5, while in the other line (11.5), the amount of siRNA produced was significantly smaller. Bioassays were conducted with both lines, but only the line Bt-22.5 was associated with significant mortality of adult insects (97% when insects were fed on detached leaves and 59% on the whole plant). The expression of the Bt-vATPase gene was 50% lower (p < 0.05) in insects that fed on the transgenic line Bt-22.5, when compared to non-transgenic controls. The transgenic line did not affect the virus transmission ability of the insects. Moreover, no effect was observe on the reproduction of non-target organisms, such as the black aphid Aphis craccivora, the leafminer Liriomyza sp. and the whitefly parasitoid Encarsia formosa. The results presented here serve as a basis for the development of whitefly-tolerant transgenic elite common bean cultivars, with potential to contribute to the management of the whitefly and virus diseases. MenosCommon bean (Phaseolus vulgaris L.) is a staple food in Brazil with both nutritional and socioeconomic importance. As an orphan crop, it has not received as much research attention as the commodity crops. Crop losses are strongly related to virus diseases transmitted by the whitefly Bemisia tabaci, one of the most important agricultural pests in the world. The main method of managing whitefly-transmitted viruses has been the application of insecticides to reduce vector populations. Compared to chemical vector control, a more sustainable strategy for managing insect-borne viruses is the development of resistant/tolerant cultivars. RNA interference has been applied to develop plant lines resistant to the whitefly in other species, such as tomato, lettuce and tobacco. Still, no whitefly-resistant plant has been made commercially available to date. Common bean is a recalcitrant species to in vitro regeneration; therefore, stable genetic transformation of this plant has been achieved only at low frequencies (<1%) using particle bombardment. In the present work, two transgenic common bean lines were obtained with an intron-hairpin construct to induce post-transcriptional gene silencing against the B. tabaci vATPase (Bt-vATPase) gene, with stable expression of siRNA. Northern blot analysis revealed the presence of bands of expected size for siRNA in leaf samples of the line Bt-22.5, while in the other line (11.5), the amount of siRNA produced was significantly smaller. Bioassays we... Mostrar Tudo |
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Palavras-Chave: |
Dry bean; Insect pest management; VATPase. |
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Thesagro: |
Bemisia Tabaci; Feijão; Mosca Branca; Phaseolus Vulgaris; Planta Transgênica. |
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Thesaurus NAL: |
Beans; RNA interference; Transgenic plants. |
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Categoria do assunto: |
--
X Pesquisa, Tecnologia e Engenharia |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1146488/1/fpls-984804.pdf
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Marc: |
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024 7 $ahttps://doi.org/10.3389/fpls.2022.984804$2DOI
100 1 $aFERREIRA, A. L.
245 $aWhitefly-tolerant transgenic common bean (Phaseolus vulgaris) line.$h[electronic resource]
260 $c2022
520 $aCommon bean (Phaseolus vulgaris L.) is a staple food in Brazil with both nutritional and socioeconomic importance. As an orphan crop, it has not received as much research attention as the commodity crops. Crop losses are strongly related to virus diseases transmitted by the whitefly Bemisia tabaci, one of the most important agricultural pests in the world. The main method of managing whitefly-transmitted viruses has been the application of insecticides to reduce vector populations. Compared to chemical vector control, a more sustainable strategy for managing insect-borne viruses is the development of resistant/tolerant cultivars. RNA interference has been applied to develop plant lines resistant to the whitefly in other species, such as tomato, lettuce and tobacco. Still, no whitefly-resistant plant has been made commercially available to date. Common bean is a recalcitrant species to in vitro regeneration; therefore, stable genetic transformation of this plant has been achieved only at low frequencies (<1%) using particle bombardment. In the present work, two transgenic common bean lines were obtained with an intron-hairpin construct to induce post-transcriptional gene silencing against the B. tabaci vATPase (Bt-vATPase) gene, with stable expression of siRNA. Northern blot analysis revealed the presence of bands of expected size for siRNA in leaf samples of the line Bt-22.5, while in the other line (11.5), the amount of siRNA produced was significantly smaller. Bioassays were conducted with both lines, but only the line Bt-22.5 was associated with significant mortality of adult insects (97% when insects were fed on detached leaves and 59% on the whole plant). The expression of the Bt-vATPase gene was 50% lower (p < 0.05) in insects that fed on the transgenic line Bt-22.5, when compared to non-transgenic controls. The transgenic line did not affect the virus transmission ability of the insects. Moreover, no effect was observe on the reproduction of non-target organisms, such as the black aphid Aphis craccivora, the leafminer Liriomyza sp. and the whitefly parasitoid Encarsia formosa. The results presented here serve as a basis for the development of whitefly-tolerant transgenic elite common bean cultivars, with potential to contribute to the management of the whitefly and virus diseases.
650 $aBeans
650 $aRNA interference
650 $aTransgenic plants
650 $aBemisia Tabaci
650 $aFeijão
650 $aMosca Branca
650 $aPhaseolus Vulgaris
650 $aPlanta Transgênica
653 $aDry bean
653 $aInsect pest management
653 $aVATPase
700 1 $aFARIA, J. C. de
700 1 $aMOURA, M. da C.
700 1 $aZAIDEM, A. L. de M.
700 1 $aPIZETTA, C. S. R.
700 1 $aFREITAS, E. de O.
700 1 $aCOELHO, G. R. C.
700 1 $aSILVA, J. F. A. e
700 1 $aBARRIGOSSI, J. A. F.
700 1 $aHOFFMANN, L. V.
700 1 $aSOUZA, T. L. P. O. de
700 1 $aARAGÃO, F. J. L.
700 1 $aPINHEIRO, P. V.
773 $tFrontiers in Plant Science$gv. 13, 984804, Aug. 2022.
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Registro original: |
Embrapa Arroz e Feijão (CNPAF) |
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